To summarize the latest study results about anti-angiogenic therapy for colorectal cancer (CRC) reported in the 13th world congress on gastrointestinal cancer, and review the latest progress of bevacizumab in treating colorectal carcinoma combined with related literatures.From the internal medicine point of view, bevacizumab is emphasized that to be applied earlier would gain benefit as soon as possible,and to be applied continuously would gain more benefit.The curative effect of second-line therapy has been confirmed renewedly.In the surgery point, bevacizumab neoadjuvant treating liver metastases in metastatic colorectal carcinoma (mCRC) can improve the disease-free rate and the operable rate significantly, and has favorable tolerance.In addition,bevacizumab can decrease the hepatic injury induced by chemotherapy safely and effectively.

Objective: To develop a method in the determination of somatostatin level in blood for clinical medication. Methods: A method for the determination of somatostatin(Octreotide) in serum by high performance liquid chromatography with diode-array detector(DAD) was developed.After ultrafiltration,the pH values of blood samples were adjusted to 4,and samples were analyzed by Kromasil C18 column(4.6 mm? 250 mm,5 ?m i.d.) with a large pore(300) by acetonitrile/water/trifluoroacetic acid(20/80/0.01%,v/v) as mobile phase. Results:The recovery rate of Octreotide was 85%,and the calibration curve showed good linearity in the range of 0.006-0.5 mg/mL. Conclusion: The method is suitable for the detection of Octreotide content by liquid chromatography.

In this paper,the author preliminarily discusses significance for strengthening the standard work of ethnic crude drugs in China,and summarizes overall achievements and insufficiency of this work.Further,the authors puts forward his own points about the scope or conditions of the crude drugs recorded in national or local drug standards,the main principle of quality standards formulation,as well as several problems that should be paid attention to in the actual research.Moreover,the author puts forward some beneficial suggestions about strengthening the standardization building of ethnic crude drugs in China.

AIM: We developed the formulated medicine WuJi Capsules into new Chinese patent medicine. METHODS: To use the pharmacodynamic test for relieving pain, stanching bleeding anti-inflammation, uters level and smooth muscle. To part from body's uterus of rat contrack, “anti-weak negative” and a long-term toxicity reaction. the maximal tolerate dose. etc. RESULTS: Wu Ji Capsules's high does group. mliddle dose group and low dosc group in the tcsts of rclicving pain stanching bleeding had extraordinary notable difference. In the part of anti-inflammation mouse's. High dose group(5.0 g?kg -1)had extraordinary notable difference and others dose groups all had notable difference. Rat's high dose group(4.0 g?kg -1)and low dose group(2.0 g?kg -1)had extraorainary notable differernce in the 1st hour from the 5th and the 24th hours. CONCLUSION: Both Wu Ji Capsules and Wu Ji Oral Liquid have significent function of anti-inflammation. For the womb of rat has contracted function, the fregwencg (P

High mobility group N (HMGN),one member of the high mobility group superfamily,is expressed ubiquitously in living cells.HMGN can bind directly to nucleosomes and modulate the structure of the chromatin fiber,which affect the cellular transcription profile and cellular differentiation and the ability to repair damaged DNA.The occurence of tumor is closely related to the abnormal transcription caused by accumulation of mutations.Abnormal transcription can prompt the tumor cells to escape from the tight regulation of cell cycle progression.Studies show that HMGN plays an important role in cancer progression by involving in the regulation of tumor cell cycle and affecting the biological behavior of tumor cells.

Objective To evaluate the role of genetic factors in the pathogenesis of vitiligo. Methods A questionnaire survey was performed to collect clinical data on 1005 cases of vitiligo in Beijing Hospital from September 1997 to March 2009. Data analysis was carried out by SPSS 13.0 software. Results There were 206 (20.5%) patients with positive family history among the 1005 cases of vitiligo. The mean age at onset was younger and bilateral vitiligo lesions were more frequently observed in patients with positive family history than those without (24.45 ± 15.87 years vs 28.12 ± 16.88 years, 71.3% vs 60.8%, both P ＜ 0.05). Blood type B predominated among patients with positive family history (19.9%), while blood type O among those without ( 16.8% ), and no statistical difference was noted in the distribution of blood type between the two groups of patients (P ＞ 0.05). Conclusions ①The incidence of vitiligo accords with the rules of polygenic inheritance and shows a familial aggregation, and the closer the blood relationship, the higher the incidence of vitiligo.②The incidence of vitiligo seems unrelated to sex. ③The mean age at onset is younger in patients with positive family history than those without, but appears unaffected by paternal or maternal inheritance. ④Patients with positive family history show a higher frequency of bilateral distribution of vitiligo lesions, but no difference in other clinical manifestions from patients without.

Objective To investigate the side effects of interferonαin treatment of chronic hepatitis B,and the analysis of its clinical significance. Methods 164 cases of patients with chronic hepatitis B were selected as research objects,who received medical treatment in Hospital of Jilin Agricultural University from December 2010 to December 2011.Clinical data of these patients was retrospectively analyzed.Adefovir dipivoxil was applied to 78 patients(group A),and peginterferon-α(PEG)was used in 86 patients(group B).Then efficacy and side effects of two interferon drug were observed.Results The HBsAg and HBV DNA negative rates of patients in group B was 4.65% and 59.30%,respectively.The negative rates of group A were 2.56% and 43.03%.HBV DNA negative rate of both groups had a significant difference by statistics(P<0.05 ),and improving effect of liver function of group B was also better than group A.After receiving IFN-αtreatment,patients of both groups all appeared side effects,including fever, leukopenia,neutropenia,depression and insomnia.The side effects incidence of both groups didn't show significant difference.Conclusion IFN-αis an effective antiviral drugs for chronic hepatitis B.long-acting interferon has a better efficacy,but this drug also lead to side effect easily.Therefore,it is essential to strengthen the relevant prevention and care in the clinical treatment.

Objective To realize high efficient and modern management of hospital library.Methods PowerBuilder programming language and SQL Server2000 database were used to construct the system.Results The system provided a convenient management platform with high efficiency,and preliminarily realized the information-based management of library.Conclusion The use of this system accelerates the information-based construction of our hospital library,and offers new approach and idea.

OBJECTIVETo explore the possible neurophysiologic mechanisms of propofol and N-methyl-D- aspartate (NMDA) receptor antagonist against learning-memory impairment of depressed rats without olfactory bulbs.

METHODSModels of depressed rats without olfactory bulbs were established. For the factorial design in analysis of variance, two intervention factors were included: electroconvulsive shock groups (with and without a course of electroconvulsive shock) and drug intervention groups [intraperotoneal (ip) injection of saline, NMDA receptor antagonist MK-801 and propofol. A total of 60 adult depressed rats without olfactory bulbs were randomly divided into 6 experimental groups (n=10 per group): ip injection of 5 ml saline; ip injection of 5 ml of 10 mg/kg MK-801; ip injection of 5 ml of 10 mg/kg MK-801 and a course of electroconvulsive shock; ip injection of 5 ml of 200 mg/kg propofol; ip injection of 5 ml of 200 mg/kg propofol and a course of electroconvulsive shock; and ip injection of 5 ml saline and a course of electroconvulsive shock. The learning-memory abilities of the rats was evaluated by the Morris water maze test. The content of glutamic acid in the hippocampus was detected by high-performance liquid chromatography. The expressions of p-AT8Ser202 in the hippocampus were determined by Western blot analysis.

RESULTSPropofol, MK-801 or electroconvulsive shock alone induced learning-memory impairment in depressed rats, as proven by extended evasive latency time and shortened space probe time. Glutamic acid content in the hippocampus of depressed rats was significantly up-regulated by electroconvulsive shock and down-regulated by propofol, but MK-801 had no significant effect on glutamic acid content. Levels of phosphorylated Tau protein p-AT8Ser202 in the hippocampus was up-regulated by electroconvulsive shock but was reduced by propofol and MK-801 alone. Propofol prevented learning-memory impairment and reduced glutamic acid content and p-AT8Ser202 levels induced by electroconvulsive shock.

CONCLUSIONElectroconvulsive shock might reduce learning-memory impairment caused by protein Tau hyperphosphorylation in depressed rats by down-regulating glutamate content.

Animals ; Depression ; psychology ; Dizocilpine Maleate ; pharmacology ; Electroshock ; Glutamic Acid ; analysis ; Learning Disorders ; prevention & control ; Male ; Memory Disorders ; prevention & control ; Phosphorylation ; Propofol ; pharmacology ; Rats ; Rats, Sprague-Dawley ; tau Proteins ; metabolism

AIM: To compare the efficacy of different locations scleral tunnel incision in phacoemulsification cataract on preoperative corneal astigmatism.
METHODS:Totally 90 patients (94 eyes) in our hospital who had undergone the phaco-surgery from March 2013 to October 2014 were divided into two groups. The group A was those with 3. 2mm scleral tunnel incision at the direction of 10:00 ~11:00 points. The group B was those with 3.2mm scleral tunnel incision at the steepest corneal meridian. Incision was not sutured. Corneal astigmatism status of preoperative and postoperative at different times were detected by corneal refractive. The impact of different surgical incision on postoperative corneal astigmatism was compared. In the two groups, patients with preoperative corneal astigmatism<1. 00D, 1. 00 ~2.00D and > 2. 00D were compared respectively. The changes of the uncorrected visual acuity and corneal astigmatism at postoperative 1wk, 1 and 3mo were observed.
RESULTS:The uncorrected visual acuity of the patients who were in the group B preoperative corneal astigmatism <1. 00D and 1. 00 ~ 2. 00D was better than that in the group A. The postoperative corneal astigmatism of the patients who were in the group B preoperative corneal astigmatism <1. 00D was lower than that in the group A.
CONCLUSION: On the basis of preoperative corneal astigmatism, 3. 2mm scleral tunnel incision at the steepest corneal meridian to some extent can correct preoperative corneal astigmatism < 1. 00D, and accordingly improve the uncorrected visual acuity.