Objective To investigate p38 MAPK signal transduction pathway of cytokine release in co-culture of BEAS-2B cells and eosinophils.Methods Eosinophils in human peripheral blood were isolated using anti-CD16 magnetic micro beads.Co-culture of BEAS-2B cells and eosinophils was taken as experimental model to investigate the inhibitive effect of SB 203580,a selective inhibitor of p38 MAPK,on release of IL-6,IL-8,IP-10 and MIG in culture supernatant.IL-6 was determined by ELISA kit and IL-8,IP-10 and MIG were measured using cytometric bead array(CBA)kit.Results SB 203580 could inhibit IL-6 and IL-8 release from BEAS-2B cells(P

Obsjective To investigate the effect of signal transduction pathway of p38 mitogen-activated protein kinase(p38MAPK) in epithelial cells activated by eosinophils on the expression and release of inflammatory mediators during co-culturing of two types of cells.Methods Eosinophils were isolated from human peripheral blood with magnetic micro beads conjugated CD16 antibody,LS separation column and magnetic cell sorting system(MCSS);The proteins of phospho-p38 MAPK and phospho-ATF-2 in BEAS-2B cells were analyzed by Western blot and enzyme-linked immuno sorbent assay(ELISA);The activity of phospho-p38MAPK was assayed by micro-bead Immunoprecipitatioin assay and Western blot.Results The interaction of eosinophils and BEAS-2B cells in co-culturing was found to elevate the protein expression and the activity of phospho-p38 MAPK in BEAS-2B cells;Phosphorylation of ATF-2 protein,a transcription factor that could regulate genes to express in cells and a substrate of phospho-p38 MAPK in the cascades of p38 MAPK signal transduction pathway was increased and it could be effectively suppressed by SB 203580,a selective inhibitor of p38 MAPK;eosinophils fixed with paraformaldehyde could also activate BEAS-2B cells to increase the phosphorylation of p38 MAPK protein.Conclusion To summarize the above results,it is showed that BEAS-2B cells activated by co-culturing with eosinophils to express and release inflammatory mediators are,at least partly,regulated by p38 MAPK signal transduction pathway.