1.Research in operating room nursing technology level-division admittance management system
Ronghuo WU ; Dan ZHU ; Zhicong ZHU
Chinese Journal of Practical Nursing 2011;27(33):7-11
Objective To construct the operating room nursing technology level-division admittance management system.Methods Adopting on-the-spot investigation,iterature research method and expert consultation method to construct the operating room nursing technology level-division admittance management system.Results The enthusiasm coefficient of selected experts was 94.7%,the average authority coefficient was 0.85,six types including 23 items of operating room nursing technology were established.The average difficulty value of this index was 5.36,the highest average difficulty value of class A surgery was 8.25,while the lowest environmental assessment of surgery was 3.21.According to the degree of difficulty,the operating room nursing technology was divided into three classes,the personnel qualifications,training content and capacity requirements of each categories of surgery was confirmed through expert consultation.Conclusions The establishment of the operating room nursing management system can enrich the connotation of operating room nursing technology,increase the nursing safety of the operating room and provide reference basis for admittance management system.At the same time,it can speed up the professionalization process of operating room nursing.
2.Microsurgical management of premalignant and malignant lesions of the conjunctiva
Huasheng YANG ; Zhongyao WU ; Siming AI ; Yuxiang MA ; Zhicong CHEN
Chinese Journal of Microsurgery 1998;0(01):-
Objective To evaluate the methods and efficacy of microsurgical treatment for the premalignant and malignant lesions of the conjunctiva. Methods Forty-seven patients witn premalignant and malignant lesions of the conjuncitva were managed by microsurgical, these patients include 12 melanoma, 26 squamous cell carcinoma, 6 Bowen’ diseases, 3 primary acquired melanosis. The surgical method differs with limbal tumors, extralimbal tumors, and primary acquired melanosis. Results In all 47 patients, the tumor was completely removed in in one procedure. After follow-up for 4~60 months(mean 17 months), these were no tumor recurrence. Conclusion It is effective methods that premalignant and malignant lesions of the conjunctiva are managed by microsurgical resection, alcohol application, and supplemental cryotherapy. Careful propeirativa clinical evaluation of patient with a conjunctiva neoplasm is important in making the correct diagnosis and planning the surgical approach.
3.Study on Immunological Components and Their Contents in Subcutaneous Exudate Induced by Cutaneous Scraping Method
Zhicong WU ; Shiya LIU ; Lianghui LI ; Jiejing LAI ; Lixian HUANG ; Hua CHEN ; Zaoyuan KUANG ; Biaoyan DU
Journal of Guangzhou University of Traditional Chinese Medicine 2017;34(2):209-212
Objective To investigate the changes of the immunological factors in subcutaneous exudate and blood components of the rats receiving cutaneous scraping method,and to compare the changes of skin histopathological features before and after cutaneous scraping under microscope.Methods SD rats were randomly divided into two groups,cutaneous-scraping group and non-cutaneous-scraping group.And then each group was divided into three subgroups.The observation indexes included the levels of interleukin (IL)-1β,IL-6 and interferon gamma (IFN-γ) in the blood and the skin,routine blood examination,and skin histopathological features.Results In cutaneous-scraping group,the number of white blood cells in the blood and the levels of IL-1 β and IFN-γ in skin tissues were increased (P < 0.05),the hemolysis rate was increased (P < 0.05).However,the levels of IL-1β,IL-6 and IFN-γin the blood showed no obvious changes.Under the microscope,severe skin edema,vascular congestion and dilatation,and infiltration of inflammatory cells were found in the skin after cutaneous scraping.Conclusion The cutaneous scraping method can activate the immune response rapidly,and the immunological components of the subcutaneous exudate after cutaneous scraping are helpful to the disease treatment.
4.Inhibitory effects of nm23-H1 gene on proliferation and invasion of A549 cell line
Qiuying LIU ; Zhicong WU ; Hongmei HU ; Sheng XIONG ; Meiying ZHANG ; Yin YUAN ; Meili LIU ; Yifei WANG
Chinese Journal of Pathophysiology 2000;0(07):-
AIM: To study the inhibitory effects of nm23-H1 gene on proliferation and invasion of human lung adenocarcinoma A549 cell line. METHODS: Recombinant eukaryotic expression vector pcDNA3.1-nm23-H1 containing full length of human nm23-H1 cDNA was constructed and transfected into a human lung adenocarcinoma A549 cell line by lipofectamine. Cell strain that expressed nm23-H1 stably was screened out by G418 and named pcDNA-nm23-A549. Expression of nm23-H1 was identified by RT-PCR and immunohistochemistry. Growth curves were drawn to detect the inhibitory effects on cell proliferation. Cell cycle of pcDNA-nm23-A549 was examined by flow cytometry. Atomic force microscopy was used to observe the filopodia on the surface of the cells. RESULTS: Introduction of nm23-H1 obviously inhibited the proliferation of A549. Expression of nm23-H1 did not induce apotosis in A549 cells but increased the percentage of phase G_1 cells and decreased phase S cells. Meanwhile, phase G_1 to phase S transition was restrained. Filopodia in the cell surface was much fewer and its structure changed in cells transformed. CONCLUSION: nm23-H1 is capable of inhibiting A549 proliferation and decreasing its metastatic ability, probably by interfering with cell cycle and cell surface structure.
5.Chemical constituents from aerial parts of Fallopia dumetosum.
Zhicong WU ; Liwei GE ; Chaofeng ZHANG ; Mian ZHANG
China Journal of Chinese Materia Medica 2011;36(13):1776-1778
OBJECTIVETo study the chemical constituents from aerial parts of Fallopia dumetosum.
METHODThe 80% ethanol extract was separated by means of silica gel and Sephadex LH-20 column chromatography. The compounds isolated from the plant were identified by physicochemical properties and spectroscopic evidence.
RESULTTen compounds were obtained and identified as emodin (1), physcion (2), quercetin (3), luteolin (4), 5"- methoxy-hydnocarpin (5), ethylparaben (6), protocatechuic acid (7), (2S,3S, 4R) -sphinganine-(2'R) -delta5'6' (E) -2'-hydroxyl tetracosanoylamino (8), beta-sitosterol (9), and daucosterol (10).
CONCLUSIONAnthraquinones and flavones were the major constituents of this plant. All compounds were isolated firstly from this plant, and compounds 5, 7, and 8 were isolated from the genus Fallopia for the first time.
Anthraquinones ; chemistry ; isolation & purification ; Flavones ; chemistry ; isolation & purification ; Plant Components, Aerial ; chemistry ; Plant Extracts ; chemistry ; Plants, Medicinal ; chemistry ; Polygonaceae ; chemistry
6.Epidemiological survey and analysis on an outbreak of gastroenteritis due to water contamination.
ZhiCong YANG ; XinWei WU ; TieGang LI ; MeiXia LI ; Yi ZHONG ; YuFei LIU ; Zhiai DENG ; Biao DI ; Cong HUANG ; HuiYing LIANG ; Ming WANG
Biomedical and Environmental Sciences 2011;24(3):275-283
OBJECTIVETo document the investigation and control of an outbreak of gastroenteritis in City G, South China, and provide a reference for preventing future outbreaks.
METHODSAn ambispective cohort study was designed. Attack rate (AR) and relative risks (RR) were calculated to identify the causes of gastroenteritis. Investigations using questionnaires included personal interviews with patients and doctors, reviews of medical records, laboratory examinations of fecal specimens and continuous hygiene monitoring of water samples from the waterworks.
RESULTSOverall, 427/71534 (AR=5.97%) cases were identified between October 31 and November 12 2010. Geographic distribution was highly localized, with 80% of cases occurring in the areas supplied by waterworks-A. Consumption of water provided solely by waterworks-A was found to be associated with illness (RR=8.20, 95 CI%:6.12-10.99) compared with that from waterworks-B. Microbiological analyses confirmed the presence of Norovirus in six of eight fecal samples from symptomatic patients, two water samples from waterworks-A and two sewage samples. After taking effective measures, the hygienic indices of waterworks-A met health criteria again on November 9 and no cases were reported 3 days later.
CONCLUSIONThe outbreak reported here was caused by drinking tap water contaminated with sewage at the source. Early identification of possible contamination sources and awareness of changes that might negatively impact water quality are important preventive measures to protect public health.
Adolescent ; Adult ; Aged ; Child ; Child, Preschool ; Disease Outbreaks ; Female ; Gastroenteritis ; epidemiology ; etiology ; Humans ; Infant ; Male ; Middle Aged ; Water Pollution ; adverse effects ; Young Adult
7.Clinical characteristics and risk factors for falls within two years after stroke in elderly patients
Yuqiu LUO ; Xiaoqing DENG ; Caikui WU ; Lixiang ZHANG ; Yanju FENG ; Zhicong CHEN ; Lihua HUANG ; Lixin XU ; Chunqiong LING ; Baojuan SHI ; Cailan WEI
Chinese Journal of Geriatrics 2018;37(9):978-983
Objective To examine the incidence ,clinical characteristics ,and risk factors for falls within two years after stroke in elderly patients. Methods A total of 365 elderly stroke patients from the Department of Neurology at the 8th Nanning People's Hospital were recruited from June 1 , 2013 to December 31 ,2014. They were divided into a fall group and a non-fall group and were followed up for two years. The incidence and clinic characteristics of falls were analyzed. The risk factors for falls were analyzed by multiple Logistic regression analysis. Results Of the 365 stroke patients included in this study ,falls were observed in 146(40.2% )patients. The interval between the stroke and the first fall :72(49.3% )patients had the first fall within 3 months;22(15.1% )occurred between 4 and 6 months;20 (13.7% )between 7 and 12 months ;17 (11.6% )between 13 and 18 months ;and 15 (10.3% )between 19 and 24 months.A hundred and five(71.9% )patients fell during daytime and 41 (28.1% )patients during night.Eighteen(12.3% )patients had one fall ;65(44.5% )patients fell 2 to 4 times ;60(41.1% )patients fell 5 to 10 times ;and 3(2.1% )patients fell over 10 times.A total of 709 falls were observed.Places of falls :102(69.9% )falls happened indoors and 44(30.1% )falls occurred outdoors.Circumstances of falls :27 (18.5% )patients fell when turning over ;23 (15.8% )fell when rising from a seating position ;4(2.7% )patients fell when showering ;15(10.3% )patients fell while standing ;9(6.8% )fell when turning around ;56(38.3% )fell while walking ;and 12(8.2% )fell while climbing the stairs or running.The severity of falls :52(35.6% )patients had no injury ;78(53.2% ) suffered soft tissue injury ;16 (11.0% )had fractures ;and 78 (53.2% )had fear of falling.Multiple Logistic regression analysis showed that age(OR=2.41 ;95% CI :1.69-3.05) ,history of falls(OR =2.85 ;95% CI :1.46-3.81) ,history of stroke(OR=1.87 ;95% CI :1.12-2.79) ,right hemiplegia(OR=2.37 ;95% CI :1.62-4.59) ,left hemiplegia(OR= 2.47 ;95% CI :1.46-4.78) ,paraplegia(OR= 2.55 ;95% CI :1.57-4.98) ,visual impairment(OR=2.35 ;95% CI :1.35-6.62) ,apraxia(OR=2.53 ;95% CI :1.42-5.63) ,unilateral spatial neglect (OR=3.34 ;95% CI :2.82-6.34) ,use of psychotropic medications (OR= 1.76 ;95% CI :1.11-1.98) ,impaired physical mobility (OR = 1.58 ;95% CI :1.82-2.91) ,low MMSE scale(OR = 3.42 ;95% CI :1.38-7.41) ,low Barthel Index score(OR = 2.83 ;95% CI :0.97-4.68) ,BBS scale<45(OR=2.48 ;95% CI :1.27-4.18) ,TUG>15seconds(OR=3.56 ;95% CI :1.91-5.23) ,and lack of rehabilitation therapy (OR=3.42 ;95% CI :1.38-7.41)were independent predictors for falls(all P<0.05). Conclusions Falls are common among elderly patients within two years after stroke.Most falls happen indoors ,during daytime and while moving.Age ,history of falls ,history of stroke ,hemiplegia ,visual impairment ,apraxia ,unilateral spatial neglect ,use of psychotropic medications ,walk with a walker ,low MMSE scale ,low Barthel Index score ,BBS scale<45 ,TUG>15 seconds ,and lack of rehabilitation therapy are independent risk factors for falls after stroke.
8.The incidence and risk factors for hip fractures in elderly patients within two years after stroke onset
Xiaoqing DENG ; Yuqiu LUO ; Caikui WU ; Lixiang ZHANG ; Fang FANG ; Yanju FENG ; Zhicong CHEN ; Lihua HUANG ; Lixin XU ; Chunqiong LING ; Baojuan SHI ; Cailan WEI
Chinese Journal of Geriatrics 2020;39(2):159-163
Objective:To investigate the incidence, clinical characteristics and risk factors for hip fractures in patients within two years after stroke onset.Methods:A total of 332 persons with first-onset stroke from the neurology department of our hospital between 1 June 2013 and 31 December 2014 were recruited and were divided into the hip fracture group and the non-hip fracture group.Clinical characteristics were recorded.Vision was tested as normal or impaired.Patients were accessed by the National Institutes of Health Stroke Scale(NIHSS), Behavioral Inattention Test, Baking Tray Task, Mini-Mental State Examination(MMSE), Birgitta Lindmark(BL)motor assessment scale, Berg Balance Scale(BBS), Timed Up & Go(TUG)Scale, and Stops Walking When Talking(SWWT)Scale.The clinic characteristics and risk factors for hip fractures were compared between the two groups after a 2-year follow-up.The accuracy of risk factors for fracture prediction was assessed by the sensitivity, specificity, and positive and negative predictive values.Results:Of 332 patients with stroke, 16 cases fractured their hips within two years after stroke onset, which corresponded to an incidence of 33‰/year(95% CI: 15‰/year-50‰/year). The 2-year mortality rate was 44%(95% CI: 25%-60%)and 48%(95% CI: 42%-54%)in patients with and without hip fractures respectively( χ2=0.036, P=0.724). The mean survival time for patients with and without hip fracture was 2.72 years(95% CI: 1.45-2.79)and 2.21 years(95% CI: 1.48-2.34)respectively.The proportions of patients with previous fractures history( χ2=16.780, P=0.041)and impaired vision( χ2=11.210, P=0.027), MMSE scale score( U=14.220, P=0.031), TUG ≥ 15 s( χ2=18.560, P=0.000)were higher, and SWWT( χ2=20.340, P=0.000)was lower in the hip fracture group than in the non-hip fracture group.The negative predictive values of previous fractures history, impaired vision, TUG and SWWT were higher than their positive predictive value.The specificities of previous fractures history, impaired vision, and SWWT were higher than their sensitivities.And the sensitivity of TUG was higher than its specificity. Conclusions:Hip fractures after stroke are common in elderly patients.Fractures often occur during daytime at home in daily activities.The previous fractures history, visual and cognitive dysfunction and impaired functional mobility are risk factors for hip fractures.We should take measures to prevent falls according to the relevant factors.Among the test scales, the timed up & go(TUG)scale could much more accurately identify patients at high risk for hip fractures.
9.Research advances in the therapeutic potential of xanthine oxidoreductase inhibitors for periodontitis
LI Yongshan ; WU Zhicong ; WANG Zixing ; YU Xihuang ; LIU Xi ; YU Ting
Journal of Prevention and Treatment for Stomatological Diseases 2023;31(12):901-906
Periodontitis is associated with abnormal purine metabolism, which is manifested by increased uric acid in host blood and increased expression of the purine-degrading enzyme, xanthine oxidoreductase (XOR), in periodontal tissues. Both XOR and uric acid are pro-oxidative and pro-inflammatory mediators under pathological conditions. Animal studies have found that injection of uric acid promotes the progression of periodontitis and that febuxostat (an XOR inhibitor) improves tissue destruction in periodontitis. Therefore, blocking the source of uric acid may be a therapeutic strategy to control the progression of periodontitis. In this article, the rationality of XOR inhibitors as potential therapeutic drugs for periodontitis is reviewed. The literature review results suggest that XOR inhibitors show antioxidative, anti-inflammatory, and anti-osteoclastic effects, and XOR inhibitors show clinical efficacy in the treatment of infectious, inflammatory and osteolytic diseases. Although there is no direct evidence to support the finding that XOR inhibitors can ameliorate periodontal microecological dysbiosis, these drugs can modulate intestinal microflora dysbiosis, and there is indirect evidence to support a beneficial effect of XOR inhibitors on periodontal microecological dysbiosis. In conclusion, XOR inhibitors may be used as immunomodulators for the adjuvant treatment of periodontitis by inhibiting inflammation, oxidative stress and anti-osteoclast effects.