Carotid stenosis is an important modifiable risk factor in ischemic stroke.The com prehensive diagnosis of carotid stenosis,including the comprehensive understanding of stenosis degree,plaque stability,and head and neck vessels,which has important guiding significance for the prevention and treatment of ischemic stroke.This article reviews the diagnostic values of digital subtraction angiography,sonography,magnetic resonance angiography and CT angiorgaphy in carotid stenosis.

Rapid prototyping technology, a kind of novel digital prototyping technology developed in mid-1980s based on a principle of discrete and accumulation, which fabricates a prototype by accurate accumulation of material according to the CAD models or CT data under the control of the computer without any other device. In recent years, it has shown a great application prospect in the field of orthopedics combined with three-dimensional image reconstruction of spiral CT and MRL In this article, review is given on the application development of rapid prototyping technology in orthopedics, such as organ model preparation and surgical planning, customized implant and bone tissue engineering porosity scaffold fabrication.

Liver transplantation is a valid treatment option for select patients with Hepatocellular Carcinoma HCC and end-stage liver disease. However, in approximately 20% of patients, recurrent HCC is the rate-limiting factor for longterm survival. Despite identification of clinical parameters that may stratify patients at high risk and exhaustive preoperative staging, cancer recurrence is likely the result of microscopic extrahepatic disease. With a desperate donor organ shortage, locoregional ablation techniques and resection are being employed in patients on the waiting list to serve as a bridge to OLT. Further more, some have advocated aggressive surgical resection of isolated metastasis in both the liver and extrahepatic viscera. Whether these creative strategies confer a survival advantage is unknown, requiring longterm follow-up to determine their efficacy.

Objective To summarize the method and experience of pharmaceutical care for a patient with insulin resistant. Methods The rationality of the treatment plan was analyzed by the clinical pharmacist, and the entire pharmaceutical care process was implemented to the patient. Results Doctors took the advice of clinical pharmacists, perfected the treatment plan. The treatment was safe and effective. The patient was discharged with a better health condition. Conclusion Clinical pharmacist integrates in the treatment team,and to promote the rational use of drugs.

AIM: To study the expression level of gene SNC66 in gastric cancer tissues, and investigate the relationship between the expression of SNC66 and the pathogenesis of gastric cancer.METHODS: Paired normal and cancerous paraffin-embeded tissues from 20 gastric cancer patients were tested by in situ hybridization (ISH) and in situ reverse transcription and polymerase chain reaction (ISRT-PCR)using ?-actin as a control.RESULTS: ISH and ISRT-PCR both showed that SNC66 were all strong positive expressed in 20 normal gastric mucosa.But in 20 gastric cancer tissues,the ISH showed that 8 were negative,8 were weak positive and 4 were strong positive. The ISRT-PCR showed that 6 were negative,9 were weak positive and 5 were strong positive when the PCR cycled 10 times;4 were negative and 16 were strong positive when the PCR cycled 25 times. CONCLUSION: There was significant expression reduction or defect of SNC66 in gastric cancer tissues.SNC66 should be further investigated as a gastric cancer negative relative gene.

Objective To investigate the risk factors in the outcome of neonatal pulmonary hemorrhage. Methods A total of 69 cases of neonatal pulmonary hemorrhage from January 2005 to December 2011 were studied. They were divided into 2 groups according to clinical outcome (death or alive). The data of the two groups were compared using single factor analysis. The risk factors were analyzed using multi-factor analysis. Results The death of neonates with pulmonary hemorrhage was correlated with aspiration pneumonia, coagulation abnormalities, DIC, heart failure and MPV. Multi-factor analysis showed that DIC (OR=6.90, 95%CI:1.514-31.419), heart failure (OR=9.62, 95%CI:1.710-54.150) and MPV<11 prior to pulmonary hemorrhage (OR=7.01, 95%CI:1.475-33.312) were the independent risk factors of neonatal pulmonary hemorrhage. Conclusions For the neonatal pulmonary hemorrhage with DIC, heart failure and low MPV, active intervention should be implemented.

Objective To establish a liquid chromatographic-mass spectrometric method ( LC-MS/MS method ) for determination of dextrorphan in human liver microsome. Methods LC-MS/MS was adopted with carbamazepine serving as an internal standard.The separation was performed on Agilent ZORBAX XDB-C18 column (2.1 mm×50 mm, 3.5μm), with mobile phase consisting of 0. 05% formic acid methanol-0. 05% formic acid in gradient elution. Dextrorphan and carbamazepine were detected on multiple reaction monitoring(MRM)mode by transitions from precursor to production(m/z 258.1→199.1, 237.1→194.1). Results The linear range of dextrorphan concentration was 19.22-768 960 ng.L-1(r=0.999 8), and the lowest quantification limit was 19.22 ng.L-1.The relative recoveries were 94.02%-98.74%, and the RSDs of intra-day and inter-day were within 10%.IC50 of psoralen on CYP2D6 was 0.6μmol.L-1. Conclusion The LC-MS/MS method is proved to be rapid, sensitive and reproducible, psoralen is a strong inhibitor of CYP2D6.

The military portable ECG for triage is a kind of minitype monitoring equipment for life information for the purpose that the ambulanceman carry through first aids in frontline. Life information of the wounded will be acquired exactly and quickly applying with this equipment, then quickening the triage and reducing the casualties. The system is based on C8051F310 single chip microcomputer, applying with high accuracy, low offset drift instrumentation amplifier AD620 and ultra low power LCM LMS019 to display real-time and accurate ECG signal sampled. The equipment is easy to use and inexpensive, suitable for mass requirement of our field army.

As an essential element of tissue engineering,the shape,pore size,and porosity of the porous scaffolds have direct influence on the attachment,proliferation,and differentiation of seeding cell as well as their properties.So it is important to be able to prepare an ideal porous scaffold which is able to provide the cells with an excellent micrcenvirenment in order to support their growth and differentiation.In this article,we reviewed some kinds of the preparation technologies of the porous scaffolds for tissue engineering,including fiber bonding,emulsion freeze drying,solvent casting/particulate leaching,high-pressuro processing,gas foaming/particulate leaching,thermally induced phase separation,and electro-spinning,with focus on rapid prototyping.It is our belief that the combination of the basic technologies and the rapid prototyping technology which is able to control the properties of the scaffolds and could be applied for individualized scaffold construction would be the major focus of research in the future.

OBJECTIVE To discuss the necessity of physical examination and laboratory diagnosis before operation of patients with anorectal diseases,and analyze the occupational harm of the staff.METHODS The results of physical examination and laboratory diagnosis before operation in 320 cases were reviewed,and summerized the various kinds of infectious diseases.RESULTS Forty-eight(15.00%) cases accompanied with variety classes of infectious diseases,and 39(12.19%)cases of them accompanied with transfusion-transmitted disease.CONCLUSIONS It is important to think highly of the current situation of anorectal diseases accompanied with infectious diseases,evaluate reasonably the occupational diagnosis to avoid occupational harm.