OBJECTIVE:To evaluate therapeutic efficacy and safety of chemotherapy combined with Xiaoaiping injection in the treatment of elderly patients with advanced gastric cancer. METHODS:60 elderly patients with advanced gastric cancer were collected from our department during 2012-2014,and then divided into union group and single group with 30 cases in each group. Single group was given irinotecan(CPT-11)150 mg/m2 added into 0.9%Sodium Chloride injection,and union group was addition-ally given Xiaoaiping injection 80 ml,qd,on the basis of single group. A treatment course lasted for 14 days,and both groups re-ceived 4 courses of treatment. The tumor markers,disease single rate,progression-free survival (PFS) and the incidence of ADR were compared between 2 groups. RESULTS:After treatment,the levels of CEA,CA199 and CA242 in 2 groups were significant-ly lower than before;the union group was significantly lower than the single group,with statistical significance(P<0.05). The dis-ease single rate of union group was 70.00%,which was significantly higher than that of single group (33.33%). PFS of union group was(11.23±2.17)months,which was significantly longer that of single group [(9.36±0.84)months],with statistical signif-icance(P<0.05). The incidence of leucopenia,alanine aminotransferase elevation,diarrhea,stomatitis and hand-foot syndrome in union group was significantly lower than in single group,with statistical significance(P<0.05). CONCLUSIONS:Xiaoaiping in-jection combined with irinotecan chemotherapy for advanced gastric cancer has obvious effect with a low incidence of ADR.

Objective To discuss the application value of the Nickel-Titanium patella concentrator(NT-PC) in surgical treatment of patellar fracture.Methods A retrospective analysis was made on clinical data of 32 cases of patellar fracture treated with the NT-PC in this hospital from January 2001 to December 2003.Results Re-examination of radiography at 12 weeks after surgery showed bone union was achieved in all the cases,with a smooth patellofemoral articular surface.The patella was preserved completely in all the comminuted fracture cases.According to the Lu's criteria,excellent and good outcomes were achieved in 26 and 6 cases,respectively,the rate of excellent or good results being 100%.The NT-PC was removed at 4 months ~ 2 years(mean,14 months) postoperatively without complications.Conclusions Application of NT-PC in the treatment of patellar fracture can preserve the comminuted patella completely,facilitate the reduction and fixation of the patellofemoral articular surface,and make early postoperative motions available.It is worthy of being recommended.

Objective To study basically the toxicity of Yangling surface water. Methods Research the toxicities of the water samples through seedling cultivation experiment and the micronucleus test of Vicia faba root tip cell with original wastewater, diluted by 50% and diluted by 25%. Results The results showed that the water had mutagenesis ability, meanwhile, there was a significant dose-response relationship between the amount of micronucleus and water concentration. And the results showed that Houhe pollution is the worst,then Gaogan River and Weihui River were next, and Wei River is the last. Conclusion The surface water of the investigated area has some potential adverse effects on the health of local people and land irrigation,so more attention should paid to the control and management of contamination.

This paper reviews the research progress in relations between indoor air pollution and human health for the last 20 years,and focuses on: the studies on environmental risk assessment methods; the major health effects caused by indoor air pollution; the minor and limited health effects caused by indoor air pollution; other key issues concerned.

In recent years,with the rapid development of minimally invasive spine surgery,lumbar disc herniation of minimally invasive treatment is an important progress.The operative injury is small,the duration is short and the effect is satisfactory.Correct recognition of microinvasive surgery,strictly grasp of its indication and perfect operative techniques are the keys to get good therapeutic efficacy.In this review,various methods of minimally invasive techniques and their clinical applications are summarized.

Objective To investigate the clinical significance of routine application of choledochoscope during operation of biliary duct.Methods The clinical data of 136 patients with the routine application of intraoperative choledochoscope dealing with bile duct diseases in this hospital from October 2003 to July 2009 were analyzed and summarized.Results Intraoperative choledochoscope inspection,taking stones and targeted surgery were performed in 116 cases with extrahepatic and (or) intrahepatic bile duct stones.The taking out rate of extrahepatic bile duct stones was 100% (85/85),the residual stone rates of extrahepatic and intrahepatic bile duct were 0 (0/85) and 22.6% (7/31),respectively,with the total residual stone rate was 6.0% (7/116).By using intraoperative choledochoscope,benign intrahepatic bile duct stricture was found in 13 cases,malignant extrahepatic and intrahepatic bile duct stricture in 8 cases.Four cases of hilar cholangiocarcinoma and 4 cases of common bile duct cancer,2 cases of lower segment of common bile duct polyp,3 cases of hepatolithiasis with the left hepatic bile duct carcinoma were diagnosed by biopsy via choledochoscope.Causes were confirmed by applying choledochoscope in 16 patients with obstructive jaundice.The use of choledochoscope with surgical treatment enabled benign and malignant bile duct stricture to achieve good results,without serious complications such as bile duct dilaceration,subphrenic abscess or acute cholangitis.Conclusions Using choledochoscope can tremendously reduce the residual rate of stone in biliary duct surgery,increase the definite diagnosis rate of biliary duct diseases and play a role of reasonable instruction in its treatment options.It has unique advantages in identifying causes of jaundice.It is remarkable that the role of applying choledochoscope to diagnose and cure biliary duct diseases.It should be widely used.

Inflammatory bowel disease( IBD),comprising Crohn’s disease( CD)and ulcerative colitis( UC),is a chronic intestinal inflammatory disease and its etiology has not yet been clarified. Studies showed that intestinal microbiota disorder is closely associated with the development and progression of IBD. Currently,fecal microbiota transplantation (FMT)is considered as a special organ transplantation and used in treatment of IBD,Clostridium difficile infection, irritable bowel syndrome,metabolic syndrome,etc. This article reviewed the relationship between FMT and IBD.

Objective:To evaluate the role of IL-12 and CpG as adjuvants to promote the differentiation of antigen-specific CD4~+T cells in vivo.Methods:Naive OVA-TCR-Tg CD4~+T cells were isolated from spleens and lymph nodes of DO11.10 mice.The cells were labeled with CFSE in vitro and then were adoptively transferred into normal mice.One day after transfer,mice were immunized i.v.either with OVA,OVA+IL-12 or OVA+CpG.3 days post transfer,single cell suspensions were prepared from spleens,lymph nodes and lungs.The proliferation and IFN-? expression of transferred CD4~+T cells in different tissues were assessed by FACS.Results:No proliferation of antigen-specific CD4~+T cells was observed in the absence of immunization.After immunization with OVA for 3 days,cells were proliferated.The frequency of IFN-? producing cells in spleens,lymph nodes and lungs was at a range of 0.93%～2.17%.IL-12 promoted IFN-?~+ cells(4.53%～26.79%);Moreover,CpG enhanced the frequency of IFN-?-producing cells(3.84%) by the cells only from lymph nodes.In consistent with the results reported previously,cells divided 3-5 times revealed higher frequency of IFN-?-producing cells than the rest of them.Conclusion:IL-12 and CpG as adjuvants can promote the differentiation and expression of IFN-? by antigen-specific CD4~+T cells,and thus mediate cellular immune responses.

Objective: To explore the relationship between individual's self-esteem structure and mental health. Methods: 136 middle school students were tested with Rosenberg Self-esteem Scale(SES), Implicit Association Test(IAT), Adolescent Self-rating Life Events Checklist(ASLEC) and Mental Health Test-CR(MHT-CR). Results: Compared with implicit self-esteem, explicit self-esteem correlates moderately to mental health. Results of hierarchical multiple regression with metal health as the dependent variables showed that explicit self-esteem, negative life events, explicit self-esteem?implicit self-esteem interaction, implicit self-esteem?negative life events interaction, and explicit self-esteem?implicit self-esteem?negative life events interaction is significant. Conclusion: This research supports the view that implicit and explicit self-esteem are distinct mental structures. Individual's self-esteem structure affects the relationship between negative life events and mental health.

Objective To establish the quality standards for Shufeng Zhike Extract;To improve the controll ability of the extract and ensure medicine efficacy. Methods To establish a HPLC method for simultaneous differentiation of Paeoniae Radix Alba, Saposhnikoviae Radix, Polygoni Cuspidati Rhizomaet Radix, Glycyrrhizae Radix et Rhizoma, Belamcandae Rhizoma and Bupleurum in Shufeng Zhike Extract. Phenomenex Kintex C18 column (4.6 mm×100 mm, 2.6 μm) was used. Acetonitrile-0.05%phosphoric acid was as the mobile phase in gradient elution (0–25 min, 10%acetonitrile; 25–26 min, 10%→14% acetonitrile; 26–35 min, 14% acetonitrile; 35–36 min, 14%→34% acetonitrile;36–55 min, 34%acetonitrile) at flow rate of 0.8 mL/min, and column temperature was 40 ℃. The detection wavelength was 250 nm. To establish a HPLC method for simultaneous determination of Paeoniflorin and Prim-O-glucosylcimifugin in Shufeng Zhike Extract, Phenomenex Luna C18 column (4.6 mm×150 mm, 5 μm) was used; acetonitrile-0.05%phosphoric acid (12:88) was as the mobile phase at flow rate of 1 mL/min; column temperature was 40 ℃; the detection wavelength was 250 nm. Results The chromatographic peak separation with HPLC method for simultaneous differentiate of Paeoniae Radix Alba, Saposhnikoviae Radix, Polygoni Cuspidati Rhizoma et Radix, Glycyrrhizae Radix et Rhizoma, Belamcandae Rhizoma and Bupleurumin in Shufeng Zhike Extract was clear and negative control had no interference. The linear range of Paeoniflorin was 0.035 93–2.514 8 μg (r=0.999 5), and the average recovery was 100.54%(n=6). The linear range of Prim-O-glucosylcimifugin was 0.006 7–0.67 μg (r=0.999 5), and the average recovery was 100.39%(n=6). Conclusion The established quality standards are simple, reliable, and accurate. It can rapidly identificate Paeoniae Radix Alba, Saposhnikoviae Radix, Polygoni Cuspidati Rhizomaet Radix, Glycyrrhizae Radix et Rhizoma, Belamcandae Rhizoma and Bupleurum, and suitable for rapid determination of Paeoniflorin and Prim-O-glucosylcimifugin, which can provide the basis for the quality inspection of Shufeng Zhike Extract.