It was found that the Lao Shan polsaccharide P, one of the chemical components of polystictus versicolor increased the number of acetylated low density lipoprotein ( acLDL ) receptorsin the macrophages and stimulated the binding, internalization and degradation of (125I ) acLDL by the macrophages in vitro. The effect on cell degradation of ( 125I ) acLDL was dose-effect. At 100?g/ ml of the Lao Shan polysaccharide P, the effect was maximum and the degradation of ( 125I ) acLDL increased 77% ( P

Objective Find out the self-rated health of college students majoring in physical education and the influential factors, so as to offering some evidences which can help adopting adopt some measures to improve health condition. Methods Adopt method of random sampling to investigate the application ofamong 300 college students majoring physical education. Results The total condition of self-rated health of college students majoring physical education is good. The scores of self-rated physiology, psychology, social healthy sub-table are from 56. 6 to 95.00. While the lowest score is the self-rated of psychological health. Male students got higher scores than female in all aspects except the ability of daily life and social support and positive emotion. And the Differences in psychological health, physical symptom and function are significant (P＜0.05) .Comparison of college students self-rated health condition with other areas, it found that the students in Sichuan are in middle level of all healthy aspects. Conclusions The total condition of self-rated health about college students majoring physical education is good. More attention should put on to their psychology,besides regular diet, rest, and tired in physiology health.

Objective To compare biomechanical performances of parallel plate fixation and vertical plate fixation for better clinical treatment of humeral intercondylar comminuted fractures (HICF).Methods Models of T-shaped fracture were created using an osteotome at the distal end of humerus in 6 specimens of upper limb in 3 human adult cadavers.The 2 fracture models of left and right humeri in the same cadaver were respectively fixated with parallel plating and vertical plating.On a biomaterial-testing machine,a vertical load was applied onto the fixated distal humerus at a speed of 5 N/s till it reached 2000 N.The loading was repeated 3 times.Vertical compression displacement,intercondylar separating displacement,deformation of medial and lateral condyles and vertical compression stiffness were documented for statistic analyses. Results Under the same load,parallel plating led to significantly less compression displacement,separating displacement and deformation of condyles( P ＜ 0.05) but significantly greater axial compression stiffness( P ＜ 0.05) than vertical plating. Conclusion In biomechanics,parallel plate fixation has better performance than vertical plate fixation for HICF.

Objective To analyze the clinical characteristics and prognostic factors of endometrial carcinoma in senile women, explore the optimized therapeutic modality. Method The clinic pathological characteristics and survival outcome of 268 patients of endometrial carcinoma in senile women were reviewed retrospectively, and prognostic factors affected the survival of endometrial carcinoma were evaluated. Results According to univariate analysis, therapeutic modality, surgical-pathological classification and pathological grade were closely correlated with prognosis of endometrial carcinoma in senile women(P < 0.01 ). According to COX multivariate analysis,surgical-pathological and pathological grade were independent prognostic associated-factor (P = 0.002,0.000). Conclusions There are closely correlations between prognosis and surgical-pathological classification,therapeutic modality and pathological grade,surgical-pathological classification and pathological grade are independent prognostic associated-factor. The therapy on endometrial carcinoma in senile women should connect with high risk factors and adopt integrating and individual therapeutic modality.

Objective To investigate the effect of ginsenoside-Rgl on paraurethral fascia fibroblastsmultiplication and the expression of proliferation cell nuclear antigen (PCNA) of stress urinary incontinence(SUI) women in vitro. Methods Specimens of human paraurethral fascia were obtained from 4 SUI womenduring tension-free vaginal tape (TVT) or tension-free vaginal tape-obturator (TVT-O) procedure.Fibroblasts were isolated and cultured by outgrowth technique. After reaching confluency fibroblasts weresubcultured every 5 days and cells after passage number 3 to 5 were used for assessment. The paraurethralfascia fibroblasts were treated with ginsenoside-Rgl at different concentrations (5, 10, 20 μmol/L) andfibroblnsts without Rgl were used as controL The multiplication conditions of paraurethral fascia fibroblastswere respectively detected by methyl thiazolyl tetrazolium (MTr) assay and the expression of PCNA byhistochemistry. Results ( 1 ) Compared with the control group, the growth rate of cells treated with differentconcentrations of Rgl after 72h [ (29±5 )%, (40±5 )%, (26±4)% respectively ] was significantly higher(P<0.01). (2)Compared with the control group, the stimulatory effect of Rgl on fibroblast growth wassignificant at 24 h (P<0.01), and peaked at 72 hi (29±5)% ,(40±5)%, (26±4)% respectively, P<0.01]. (3)Compared with the control group(28.77% ), there was a significant increase of PCNA-positivecells (P<0.01) after 48 h treatment with different concentrations of Rgl (49.24%, 83.48%, 54.50%respectively). Conclusion The results indicate that, at least in vitro, fibroblasts from paraurethral fasciataken from women suffering from SUI are able to proliferate after

The expression of C-myc, K-ras and H-ras oncoproteins in lymphocytesand monocytes, and NK subsets were studied in 21 patients with Behcet's syndrome (BS)and 40 healthy individuals. CD16~+,57~- cells of NK from BS patients were much decreasedwhen compared to the control. The positive percentages of three kinds of oncoprotein in BS lymphocytes and C-myc and K-ras oncoproteins in BS monocytes were significantly higher thanthat of the control. Positive correlations were found not only between C-myc expression oflymphocytes and serum ?-globulin%, IgM and IgG levels in BS, but also between C-mycexpression of monocytes and ESR. Expression of K-and H-ras oncoproteins in lympho-cytes and monocytes from BS patients were found to be negatively correlated with serumIgA level. Negative correlation was also found between C-myc expression in monocytesand CD16~+,57~- subset of NK. The significance of these results and the possible correlationsbetween BS and autoimmunity were discussed. It was suggeted that autoimmunity mightbe an important mechanism underlying BS.

Objective To evaluate the effect of lidocaine on delayed neuroal death after global cerebral ischemia. Methods Global cerebral ischemia was induced by the occlusions of bilateral commom carotid and vertebral arteries . Twenty-five rabbits were randomly divided into three groups:sham operative group (SH group,n=5) without the four-vessel occlusion; ischemia group(IS group,n=10) with intravenous normal saline of 15 ml followed by the four-vessel occlusion for 5 min; lidocaine group (LI group,n=10) with intravenous lidocaine of 10mg/kg followed by the four-vessel occlusion for 5 min. Three days later, every cerebrum was resected and stained using terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) and hematoxylin-eosin (HE) methods,to count the amount of the positive stained cells of hippocampus. Results The positive cell amount of HE staining (ischemic change cells) was correlated with that of TUNEL staining (apoptosis cells) (r=0.915).Compared with that in SH group, the amount of apoptosis cells increased significantly in IS and LI groups (P

Objective To introduce the new nomenclature scheme of the International Working Group (1995) on hepatic nodules, and summarize the imaging features of various hepatic nodules in light of their pathological characteristics, and evaluate the diagnostic values of various imaging facilities.Methods Ultrasound, computed tomography(CT), magnetic resonance imaging(MRI), and angiographic CT were reviewed and introduced.Results Many of these types of hepatic nodules play a role in the de novo and stepwise carcinogenesis of hepatocellular carcinoma(HCC) in the following steps: regenerative nodule, low grade dysplastic nodule, high grade dysplastic nodule, small HCC, and large HCC. Accompanying such transformations, there are significant alterations in the blood supply and perfusion of these hepatic nodules.Conclusion Modern state of the art medical imaging facilities can not only delineate and depict these hepatic nodules, but also provide important clues for the characterization of focal hepatic lesions in most cases, thus facilitating the early detection, diagnosis and management of HCC in its early stage.

Objective To introduce the technique of three dimensional portal venography of multi detector row spiral CT and its clinical application in the evaluation of the portosystemic collateral shunts of liver cirrhosis. Methods All relevant literatures were retrospectively reviewed on the application of two dimensional and three dimensional reconstruction techniques such as MIP, SSD, VRT of multi detector row spiral CT to demonstrate the collateral vessels of liver cirrhosis.Results The distribution, pathway and anatomy of portosystemic collateral vessels were well shown by multi detector row spiral CT portal venography. Conclusion Multi detector row spiral CT portal venography provides excellent depiction of the anatomic characteristics of the collateral shunts and enables the continuous tracing of vascular structures, thus it is very helpful in the imaging evaluation of the collateral vessels of liver cirrhosis.