母体胚胎亮氨酸拉链激酶（maternal embryo leucine zipper kinase ,MELK）在多数恶性肿瘤中高表达，其通过与各种类 型蛋白相互作用和使其磷酸化后调节靶蛋白的生物活性，影响细胞周期调控和细胞增殖、凋亡、侵袭与转移等，促进癌细胞增殖 和肿瘤形成，因此，MELK功能失调成为癌细胞逃避人体正常免疫监视的重要机制。深入了解MELK在肿瘤中的表达和作用机 制，为完善肿瘤诊断分子标志物体系、肿瘤靶向治疗方案具有一定的临床意义。本文就MELK的生物学功能、对恶性肿瘤的治疗 情况及其作为候选靶向分子的潜质进行综述。

Objective:To study the feasibility for transumbilical single-incision laparoscopic appendectomy (SILA) with conventional laparoscopic instruments, and compare SILA with the traditional three-port laparoscopic appendicectomy (LA).Methods:The clinical data of 113 patients with acute appendicitis from January 2018 to August 2020 in the Second Hospital of Jiaxing City were retrospectively analyzed. Among them, 61 patients received traditional three-port LA (three-port LA group), 52 patients received transumbilical SILA (SILA group). The operative time, intra-operative blood loss, surgical complications, length of hospital stay and hospitalization costs were recorded. Pain score 6 h after operation was assessed by visual analogue scale. C-reactive protein (CRP) on the first day after operation was detected. All patients were followed up for 1 month.Results:In 2 groups, all patients did not suffered from the conversion to open operation or multi-port method, massive bleeding and accessory injury during the operation. Moreover, severe pain, severe incision infection, residual abdominal abscess and incisional hernia did not occur. Patients in SILA group had more hidden abdominal scars. there were no statistical differences in operative time, intra-operative blood loss and CRP on the first day after operation between 2 groups ( P>0.05); the rate of moderate pain degree 6 h after operation, length of hospital stay and hospitalization costs in SILA group were significantly lower than those in three-port LA group: 15.38% (8/52) vs. 34.43% (21/61), (3.63 ± 1.22) d vs. (4.31 ± 1.38) d and (8 802 ± 1 466) yuan vs. (9 559 ± 1 617) yuan, and there were statistical differences ( P<0.05 or <0.01). Conclusions:The transumbilical SILA with conventional laparoscopic instruments is safe and feasible, the scar is more difficult to see, the cosmetic effect is much clearer, and the length of hospital stay is shorter.