Objective To explore the curative effect of loop electrosurgical excision procedure (LEEP) on cervical intra-epithelial neoplasia(CIN) infected with high risk human papilloma virus (HR-HPV).Methods 80 CIN patients with HPV infection underwent LEEP were selected.HR-HPV DNA load was detected before operation and 3 months after operation,and at the same time,vaginal examination and cervical directed biopsy were conducted.Results Before LEEP operation,the differences between different levels of CIN HPV load were not significant(P ＞0.05,F =0.45).3 months after operation,CIN Ⅰ grade 19 cases (86.4％) turned to chronic inflammation,CIN Ⅱgrade 36 cases (83.7％) turned to chronic inflammation,CINⅢ grade 13 patients (86.6％) were chronic inflammation.Cervical HPV load postoperatively negative rate was 85％ and the lower HPV load,the higher postoperatively negative rate.Conclusion LEEP is the effective treatment for cervical lesion and can significantly decrease the HPV-DNA loading at cervix.

Objective To evaluate and compare the clinical applications of Disposcope (DS)en-doscope and GlideScope (GS)video laryngoscope in double-lumen endobronchial tube (DLT)intuba-tion of the patients with difficult airway.Methods Forty patients scheduled for elective thoracic sur-gery after failure to place the DLT with modified general laryngoscope 2 attempts were randomly dev-ided into 2 groups,20 cases in each group.Patients underwent DLT with DS (group DS)or GS (group GS)intubation after failure to intubation.The causes of the failure of intubation,the intuba-tion of the video laryngoscope,the time of intubation and the location of left double lumen tube were recorded.And the postoperative injury of oral mucosa,teeth and respiratory tract were observed. Results The causes of the difficulty for DLT intubation included:long,high arched palate,large epi-glottis,reduced jaw opening,protruding or loose incisors,over bite,reduced neck extension.The in-tubating achievement ratio was significantly higher in group DS than in group GS (P < 0.05 ).But there was no obvious difference between the two groups in the location success rate and intubation time.The postoperative incidence of tooth loss and oral mucosal injury was significantly higher in group GS than in group DS (P <0.05).Conclusion Both DS and GS were great helpful to intubation and location of DLT in the patients with difficult airway.But DS was more superior than that of GS in the difficult airway of reduced jaw opening,protruding or loose incisors,over bite and reduced neck extension.

A total of 446 hyperuricemic patients were divided randomly by community-based cluster sampling into study (n =228) and comparison (n =218) groups.After 12-month health interventions,the Knowledge-Attitude-Performance (KAP) questionnaire [(19.0±4.7) points],lifestyle (balanced diet,modest physical exercises & regular monitoring) self-report questionnaire [attitudes prevention awareness:(8.3 ± 6.1) points,disease self-management behaviors:(13.2 ± 9.8) points],uricacid index [(395 ± 46)mmol/L] and body mass index (BMI) [(23.1 ± 2.3)kg/m2] were better than those at pre-intervention in study group [(9.3 ± 2.7),(4.6 ± 3.9),(7.4 ± 6.4)],[(420 ± 44)mmol/L,(24.9 ± 1.6) kg/m2] (P ＜ 0.05).Also the inter-group difference had statistical significance (P ＜ 0.05).It suggests that health intervention management is both feasible and effective in improving the KAP of patients,modulating lifestyles and lowering the levels of uric acid index and BMI.

Objective:To explore the relationship between glycosylated hemoglobin (HbA1c)and carotid artery atheroscle-rosis in aged patients with normal glucose tolerance.Methods:A total of 100 aged patients with normal glucose tolerance were selected.Their bilateral carotid arteries were measured by color Doppler ultrasonography.They were grouped accord-ing to carotid intima-media thickness (C-IMT,normal,thicken and atherosclerosis)and plaque form (no plaque,hard plaque and soft plaque);blood pressure,blood lipids and HbA1c etc.were compared among groups.Results: (1)Along with CIMT rose,there was significant increase in HbA1c level [(4.98 ± 0.55)% vs.(5.51 ± 0.42)% vs.(5.92 ± 0.39)%],and there was significant difference between any two groups (P<0.01 all);(2)HbA1c level was (5.36±0.51)%,(5.89±0.44)% and (5.97±0.2)% in no plaque group,hard plaque group and soft plaque group respectively,and there was significant difference among hard plaque group,soft plaque group and no plaque group (P <0.01 all).Conclu-sion:Glycosylated hemoglobin may become an important index assessing carotid atherosclerosis in aged people.

Glucose Intolerance ; diagnosis ; metabolism ; Humans ; Hypertension ; diagnosis ; metabolism ; Medicine, Chinese Traditional

Objective: To investigate the constituents of indole alkaloids in Ervatamia yunnanensis and their antiaddictive bioactivities. Methods: The preliminary alkaloids (TEYA) were extracted by 95 % EtOH from the stems of Ervatamia yunnanensis. TEYA was then purified by silica gel and Sephadex LH20 column chromatography repeatedly and its structure was identified with spectral analysis. The antiaddictive effects of these indole alkaloids were evaluated by Conditioned Place Preference (CPP) experiments. Results: Nine indole alkaloids were obtained and identified as: coronaridine (1) , voacangine (2), 3-R-ethoxycoronaridine (3), 3-S-ethoxycoronaridine (4), 19-epi-heyneanine (5), heyneanine (6), 19-epi-voacristine (7), coronaridine hydroxyindolenine (8) and 12-methoxyl-voaphylline (9). Among them voacangine (2) and 19-epi-voacristine (7) were effective in prevention and treatment of addiction induced by morphine in rats (n=8). Conclusion: Compounds 3, 4, 8, and 9 have been isolated from Ervatamia yunnanensis for the first time and coronaridine-type indole alkaloids are the main antiaddictive constituents of E. yunnanensis.

Objective To investigate the change of P300 of the attention - deficit/hyperactivity disorder children before and after they took litalin, according to this objective subject to guide the clinical treatment of ADHD children. Methods Using the looking se-ducible electricity stimulates 22 ADHD children, and check the change of latency and the rate of the wave before and after they took litalin. Results After ADHD children took litalin, their latency of P300 has decreased clearly, and the amplitude had no change. Conclusion The changes of P300 latency in ADHD children after they took litalin can be adopted as the guidelines of clinical treatment for the ADHD children.

Silent information regulator 1 (SIRT1) is a NAD+-dependent histone deacetylase,which plays an important role in cerebral ischemia.It mainly stabilizes cerebral vascular endothelium,prevents vascular stenosis and the anti-inflammatory and anti-oxidative stress effects after cerebral ischemia.This article reviews the protective effects of STRT1 and its related mechanisms in cerebral ischemia.

Cervical cancer is women's common malignant tumor.With the extensive application of screening and early surgery,cervical cancer incidence declines year by year,but no decrease mortality trend.The patient died of cancer metastasis and recurrence.E-cadherin is the central link of intercellular adhesion,and junction complex has been confirmed by a large number of literature and closely associates cervical cancer development.This review summarizes the relevant research between E-cadherin and the occurrence and development of cervical cancers.

Objective To investigate the effects of matrine on proliferation and apoptosis of human rhabdomyosarcoma(RMS) RD cells line in vitro,to study the regulatory mechanism of Wnt/β-catenin pathway-influenced apoptosis of RD cells line by detecting the expressions of β-catenin protein,Bcl-2 protein and caspase-3 protein,and to explore Wnt/β-catenin mechanism during the process of RMS.Methods The human RMS RD cells line was treated with matrine of different concentrations (0.5,1.0,1.5,2.0 g/L)for 48 hours respectively,and the proliferation inhibition rates of different concentrations of matrine on RD cells were detected by methyl thiazolyl tetrazolium assay,while the apoptosis rates by flow cytometry (FCM) and the expressions of β-catenin,Bcl-2 and caspase-3 by Western blot.Results The proliferation inhibition rates between control group and different concentrations of matrine groups were(13.70 ±0.25)％,(33.16 ±0.11)％,(42.96 ±0.90)％,(56.26 ±0.79)％ and (67.89 ±0.63)％,respectively.The apoptosis rates were (5.49 ± 0.96) ％,(17.23 ± 5.03) ％,(25.84 ± 4.17) ％,(36.08 ± 3.68) ％and (47.79 ± 4.82) ％,respectively.The highest expression of β-catenin and Bcl-2 proteins and the minimum amount of caspase-3 protein were found in the control group.After intervention of matrine,the expressions of β-catenin and Bcl-2 reduced while the amount of caspase-3 rose significantly,which was concentration-dependent obviously.Differences were found between every concentration of matrine group with control group according to statistics (all P ＜ 0.05).Conclusions Matrine can inhibit proliferation and induce apoptosis of RD cells.Matrine can down-regulate the expression of β-catenin and Bcl-2 proteins in RD cells,while the amount of caspase-3 protein rises.Wnt/β-catenin signal pathway plays an important role in the apoptosis of RD cell induced by matrine,and its downstream proteins Bcl-2 and caspase-3 are also involved in the regulation of this process.