0.05). In both RLC and CLC Group, postoperative levels of CRP, IL-1, IL-6, and TNF were significantly elevated as compared with preoperative levels (P

Objective:To investigate the retinal toxicity of chitosan and gentamicin- chitosan gel. Methods:Sixteen albino New Zealand rabbits (weighted 1. 5-2.5 kg) were used in this study. The rabbits were divided into 4 groups randomly;each group was injected with 0.1 ml PBS, gentamicin 2 mg/ml,3% Chitosan gel, and gentamicin-chitosan gel (2 mg/ml) respectively. ①Ophtahlmoscopic examinations were done. Retina was observed prior to injection , and on 1st, 3 rd, 7 th, 14 th d and 28 th d after injection with direct ophthalmolscope observations. ②Electroretinographs (ERGs) were recorded prior to injection, and then on 3 rd,7 th,14 th d and 28 th d after injection were carried out. ③Light microscope and transmission electronicmicroscope examinations were performed four weeks after the injection. All eyes were enucleated, the retina examples were observed under light microscope and transmission electronic microscope. Results:Intravitreous injection with 3% chitosan was free of retina toxicity. The retina toxicity effects of gentamicin 200 ?g could be found with transmission electron microscope, but gentamicin-chitosan gel with the same dosage could decrease the damage. Conclusion:The result shows that chitosan can be used in the intravitreous injection.

In addition to Routine Remedy for Angina Pectoris for all the patients. Refined Xue Fu Capsules and Xue Fu Capsules were prescribed for the first group (N = 30) and the second group (N = 34). The third group (N = 27) received only routine remedy as control in this single - blinded clinical trial. The total effective rate in these three groups were 96. 6%, 73. 5% and 70. 4%. The effect in the first group was significantly higher than the rest two groups (P

目的了解住院精神病患者并发躯体疾病的特点及解决办法。方法对住院期间并发躯体疾病的精神病患者进行调查。结果和结论住院精神病患者所患躯体疾病常涉及多个系统和器官;患者及家属对检查配合度不高,躯体疾病与精神疾病的治疗可能存在矛盾,导致住院期间并发躯体疾病精神病患者诊断、治疗困难。

Objective To investigate the development and dynamic changes of host immune response in DBA/2 mice infected with Plasmodium yoelii 17XL. Methods Female DBA/2 mice were infected by intraperitoneal ( i. p. ) injection of 106 P. yoelii 17XL parasitized erythrocytes ( PRBC). Levels of IL-12, IFN-γ, IL-4, IL-10 and P. yoelii 17XL-specific antibody in sera were measured by ELISA. Concentrations of NO in cell supernatants were measured by the Griess reaction. Parasitemia,percentage of mononuclear-macrophages of individual mice were monitored daily, and phagocytosis of mononuclear macrophages was also observed. Results Primary parasitemia in vein blood was developed on day 3 postinfection, which peaked with a level of 46. 9% on day 9. Most mice cleared the infection and survived by day 20 postinfection. From day 6 to day 16, the phagocytosis of PRBC by rodent macrophages was observed on the blood smear. Infected mice had a continuously increased level of IL-12 in serum from day 1 postinfection. Accordingly, high level of IFN-γ was also detected in sera from day 1 postinfection,which peaked on day 6. Infected mice produced higher level of IL-4 and IL-10 in serum on day 6 postinfection, which peaked on day 9 and day 15 postinfection respectively. In addition, splenocytes from infected mice produced significantly higher level of NO on day 6 and 20 postinfection. Level of P. yoelii 17XL-specific IgG was determined in the sera of infected mice with a steadily increased trend after infection, which peaked on day 70 postinfection. Conclusions Effective polarizing of Thl cells is significant in inhibition of parasitemia and eventual clearance of the Plasmodium parasites. Activated mononuclear-macrophages play a key role in inhibiting parasitemia in the early phase of infection with P. yoelii 17XL.

Objective To analyze expression of miR-324-5p in plasma of CHD patients by real-time PCR and identification of early diagnosis,for CHD.Methods In 2012 June to 2013 February 76 patients and 13 normal controls who were included in the study had measurement of plasma expression levels of miR-324-5p by real-time PCR.MedCalc 13.0 software were used for statistical analysis and comprehensive evaluation of miR-324-5p in CHD disease for diagnosis.Results Analysis of re-ceiver operating curve (ROC)showed that area under the curve (AUC)was 0.731 and best diagnostic threshold was 0.116 1.The sensitivity was 64.5% and specificity of 84.6%.Conclusion Circulating miR-324-5p as a biomarker for early diagno-sis of CHD has some-extent clinical value,but needs combined with other medical indicators.

Objective To compare the postoperative changes in immune function of patients undergoing robot-assisted laparoscopic cholecystectomy(RLC) and conventional laparoscopic cholecystectomy(CLC).Methods The clinical data of 52 cases of gallbladder stones and gallbladder polyps treated with RLC(23 cases),and with LC(29 cases) was compared.Blood samples from both groups were taken to measure immune markers(T cells,IgA,IgM,IgG,C3,C4) before operation and in the first postoperative morning.Results The postoperative levels of CD4/CD8 and IgM decreased from 1.56?0.67,1.42 ?0.58 to 1.30?0.53,1.26?0.46 respectively after RLC(all P0.05).Conclusions RLC and CLC showed no significant postoperative effect on cellular immune function,but both had some effect on humoral immune function.

Objective The aim of this study was to investigate which inflammatory markers allow an accurate differentiation of septic and gouty arthritis.Methods In 2013 January to 2014 January 33 patients with septic arthritis and 29 patients with gouty arthritis.Detected white blood cells,C-reactive protein and uric acid of inflammatory markers in plasma and tested lac-tate,glucose,uric acid,lactate dehydrogenase and white blood cell count inflammatory markers in the synovial fluid.MedCalc 13.0 software were used for statistical analysis.Results There were no significantly different between serum C-reaction protein and WBC counts with two groups.Synovial lactate showed the greatest diagnostic potential (AUC=0.898,sensitivi-ty=96.9%,specificity=72.4%)followed by serum uric acid (AUC=0.818)and synovial uric acid (AUC=0.808).Con-clusion Lactate in the synovial fluid has excellent diagnostic potential to differ septic arthritis from gouty arthritis.Synovial lactate levers above 1.7 mmol/L almost proofed septic arthritis.

Objective To research the relationship between anaemia and inflammatory in patients with heart failure .Methods 284 cases of patients with heart failure were enrolled and divided into 2 groups (anaemia group and non‐anaemia group) .The serum levels of of brain natriuretic peptide (BNP) ,high‐sensitivity C‐reactive protein (hs‐CRP) ,blood urine nitrogen (BUN) and creati‐nine (Crea) were measured ,and the results were analyzed .Results The levels of BNP ,hs‐CRP and Crea of anaemia group were sig‐nificantly higher than those of non‐anaemic group (P<0 .01) .The results of logistic regression demonstrated that hs‐CRP was in‐dependently associated with anaemia (P=0 .021) .Conclusion The occurrence and development of inflammation are independently associated with anaemia in the patients with heart failure .

Objective To study the effects of traditional Chinese medicine of Jianpizhidong decoction on dopamine D2 receptor(DAD2R) mRNA in tourette syndrome (TS) model mice brain striatum,and provide the proof of neural biochemical for Jianpizhidong decoction in curing children TS.Methods 32 male ICR mice were divided randomly into control group,model group,Jianpizhidong decoction group and Tiapride group.Except control group,all mice in model group,Jianpizhidong decoction group and Tiapride group were made to TS models by intraperitoneal injection of 3,3'-iminodipropionitrile.The control gronp and model group were given normal saline,Jianpizhidong decoction group and Tiapride group were given corresponding drugs by intragastric administration respectively.Results The striatum DATmRNA expression of model group,Tiapride group,and traditional Chinese medicine group decreased(respectively(0.139 ± 0.013),(0.15 ± 0.021),(0.141 ± 0.019)) than that of control group (0.180 ± 0.028),and with 14.1％,3.0％ and 3.9％ down respectively.In model group DATmRNA expression was lower than control group (P ＜ 0.01).In model group DATmRNA expression was lower than Tiapride group (P＜ 0.01).In model group DATmRNA expression was lower than traditional Chinese medicine group (P＜0.01).In Tiapride group DATmRNA expression was lower than traditional Chinese medicine group (P ＜ 0.01).Conclusion The function of improving symptoms of TS by Jianpizhidongtang may be relate to inhibite DAD2R hypersensitization to enhance the activity of substantia nigra-striatum.