Objective To establish a method for the determination of Ca2 + cations in the nutritive food by ion chromatography.Methods After being digested by the mixture of nitric acid and perchloric acid,the Ca2+ cations in the nutritive food were determined by ion chromatography(IC).Results The optimum chromatographic conditions were studied thoroughly,the linear range was 0.10-500 mg/L,r=0.9998.The lowest detection limit of Ca2+ cations in nutritive food was 0.02 mg/L,RSDs were 0.50%-4.00%,the rates of recovery tested by the standard addition method were in the range of 95.0%-103.0%.Conclusion This method is simple,fast,sensitive and accurate.

BACKGROUND: Balance disorder of the two neurotransmitters-dopamine and glutamic acid in striatum, participates in the pathogenic process of Parkinson disease. Whether or not electroacupuncutre can regulate the content of glutamic acid in striatum in the treatment of Parkinson disease so as to recover the balance of the two neurotransmitters is needed further investigation.OBJECTIVE: To observe the changes of content of excitatory amino acid (glutamic acid) in striatum of rat with Parkinson disease and the effects of electroacupuncture intervention on these changes.DESIGN: Randomized controlled trial.SETTING: Teaching and Research Section of Acupuncture and Moxibustion, Hubei University of Traditional Chinese Medicine.MATERIALS: The experiment was completed in Laboratory for Important Subject (Acupuncture and Moxibustion) of Hubei University of Traditional Chinese Medicine from February to August 2002. A total of 40 healthy SD rats were selected and randomly divided into 4 groups, namely, normal group, model group, sham operation group and electroacupuncutre group with 10 in each group.METHODS: Model group: Rotation model of rat with Parkinson disease was established by method of chemical damage on right substantia nigra.Sham operation group: Method of sham operation was the same as model group, and 3.4 μL physiological saline was injected. Electroacupuncture group: Electroacupuncture treatment was given after model establishment;took Atlas of Experimental Animal Acupoints as reference, selected the acupoints of Taichong and Fengfu, pierced with needles which were connected with G6805 electroacupuncture therapeutic apparatus under the condition of continuous waves and frequency of 2 Hz for 30 minutes, once a day for consecutive 7 days, then detected the content of glutamic acid.MAIN OUTCOME MEASURES: Content of glutamic acid in bilateral striatum of rat in normal group, sham operation group, model group and electroacupuncture group, which was detected by automatic amino acid analyzer.RESULTS: The content of glutamic acid in right striatum of rat in model group was significantly higher than that in contralateral striatum, normal group and sham operation group [the contents of glutamic acid in model group, contralateral striatum, normal group and sham operation group were (101.6±7.49), (71.4±5.65), (61.8±4.38), (62.6±5.21) μg/g respectively,P ＜ 0.01]. The content of glutamic acid in right striatum in electroacupuncture group was significantly lower than that in model group [the contents of glutamic acid in electroacupuncture group and model group were (83.7±5.68), (101.6±7.49) μg/g respectively, P ＜ 0.05], but still higher than that in normal group and sham operation group (P ＜ 0.05). There was no significant difference between the contents of glutamic acid in left striatum in each group (P ＞ 0.05).CONCLUSION: Excitatory amino acid participates in the pathogenic process of Parkinson disease. Therefore, one of the approaches of electroacupuncture treatnent for Parkinsonism is reducing the release of excitatory amino acid.

OBJECTIVE: To investigate laryngopharyngeal reflux effect on expression of COX-2mRNA in glottis carcinoma lesion mucosa. METHOD: Forty cases with glottic laryngeal cancer were examined by electronic naspharyngeal laryngoscope and scored by the reflux symptom index (RSI) and the reflux finding score(RFS). Based on the scores, they were divided into two groups:glottic laryngeal cancer with positive reflux(20 cases) and glottic laryn geal cancer with negative reflux (20 cases). Ten cases with adjacent normal membrane were used as control group. The mRNA expression of CoX-2 from 40 patients was examined by reverse transcription polymerase chain reaction (RT-PCR). RESULT: The expression of COX 2mRNA in tumor samples was significantly higher than that in normal tissues (P < 0.05); the expression of COX-2mRNA in glottic laryngeal cancer with positive reflux was significantly higher than that in glottic laryngeal cancer with negative reflux (P < 0.05). CONCLUSION Laryngopharyngeal reflux factors may increased expression glottic carcinoma of COX-2mRNA by tissue injury, inflammation and cell malig-
Cyclooxygenase 2 ; genetics ; metabolism ; Female ; Glottis ; Humans ; Laryngeal Neoplasms ; complications ; metabolism ; Laryngopharyngeal Reflux ; complications ; metabolism ; Male ; Mucous Membrane ; metabolism ; RNA, Messenger ; metabolism ; Reverse Transcriptase Polymerase Chain Reaction

OBJECTIVE To observe the sterilization effect of nanophotocatalytic air disinfector(NPD) on the indoor air of emergency room.METHODS Under the static and active circumstances,the on the-spot sterilization was adopted and observed.RESULTS In the static circumstances,after the machine had properly functioned over 60 minutes,the elimination rate of natural bacteria in debridement room and dressing change area reached 100.00% and 90.44%,respectively;in the active,after the machine had properly functioned over 60 minutes,the elimination rate of natural bacteria in debridement room and dressing change area reached 95.99% and 86.68%,respectively.The bacteria amount in the air decreased as the sterilization time was extended.CONCLUSIONS The NPD has good sterilization effect on indoor air.It can be adopted for the air sterilization in the emergency room under active circumstances.

Objective To investigate the possible mechanism and re-ablation of recurrent atrial arrhythmias after atrial fibrillation(AF) ablation by utilizing the Carto system and double LASSO technique.Methods A total of 77 AF patients were enrolled and among them,56 with paroxysmal AF and 21 with persistent AF.Eight-F Swartz long sheaths were positioned in the left atrium by transseptal technique.One to two LASSO catheters were placed in the ipsilateral pulmonary veins(PV).The anatomy of the left atrium and PV was established using the Carto system.PV selective venography was carried out to identify the PV ostium.Continuous circular lesions(CCL) were made at the left atrium at 0.5 to 1.0 cm outside the PV ostium.Irrigated radiofrequecy energy was delivered in all the patients.The end point of the CCL was defined as absence of all PV spikes documented with LASSO catheters within the ipsilateral PVs.Results A repeat ablation was performed in 14 patients who experienced highly symptomatic atrial tachyarrhythmia.During the repeat procedures,conduction gaps in the previous CCL were found in 13 out of the 14 patients,and a typical atrial flutter was found in the other patient which was eliminated by ablation of the tricuspid isthmus.During the second procedure,pulmonary vein tachycardia was demonstrated in 7 patients.The pulmonary vein tachycardia activated the LA via the conduction gaps.All conduction gaps were successfully ablated by irrigated RF applications.After the second procedure,12 out of the 14 patients were free of AF during 3 to 30 months of follow-up.Conclusion Continuous circular lesions(CCL) in the left atrium utilizing the Carto system and double LASSO technique had a high success.In patients with recurrent atrial tachyarrhythmia after CCLs,recovered PV conduction is a main cause for the recurrence.

Objective To investigate the effect of taurine(Tau) on the myocardial fibrosis and its mechanism.Methods The rat model of myocardial fibrosis was built by subcutaneous injection of isoprel(Iso).And the rats were divided into control group,model group and taurine group(80,160,320 mg?kg-1?d-1).Hydroxyproline measurment was carried out for detecting collagen quantification in myocardium tissue.Histopathology changes were detected by HE staining.In vitro,the cultured neonatal rat cardiac fibroblasts(CFb) were divided into control group,model group and taurine group(40,80,160mmol?L-1).The proliferation of CFb was induced by angiotensin Ⅱ(AngⅡ) and detected by the thiazoleblue(MTT)colorimetric assay.TGF-?1 level was determined by ELISA technique.The expression of cyclin dependent kinase inhibitor p27 was assessed with flow cytometry.Results In vivo,the contents of hydroxyproline were decreased in Tau(160,320 mg?kg-1?d-1) groups as compared with model group(P

A near infrared (NIR) method for the detection of illegal addition of sildenafil citrate in capsules of Chinese traditional medicine was constructed. The diffuse reflectance near infrared spectra were recorded using integrated sphere and fiber optics respectively. Using the spectra of the sildenafil citrate standard as Reference spectra, the threshold of the correlation coefficient was set up based on the minimum effective concentration of the sildenafil citrate. By comparing the threshold with the correlation coefficient between the tested spectra and the Reference spectra in the selected spectral range, the tested sample contains sildenafil citrate is determined. For the integrated sphere method, the spectral range of 6070-5800 cm~(-1) and 4170-4070 cm~(-1) were selected, and 70% was set as threshold. For the fiber method, 6070-5800 cm~(-1) and 65% were used. 79 and 247 batches of the Chinese traditional medicines contained sildenafil citrate collected from the Chinese market were used to validate the two methods separately. The accuracy of the integrated sphere method was 91.14%, and that of f iber method was 87.45%.

AIM: To assess the effect of indomethacin on tumor invasion in a human laryngeal cancer Hep-2 cell line in vitro. METHODS: Hep-2 cells were exposed to indomethacin at different concentrations for 48 h. Then cell growth rate, the colony formation in soft agar medium and cell mobility were examined, and monolayer invasion assay was performed to assess cell invasion index. RESULTS: Preteatment with indomethacin inhibited the colony formation of Hep-2 cells and the cell mobility, and decreased the invasion index. CONCLUSION: Indomethacin can inhibit the invasion of Hep-2 cells.

AIM: To evaluate the significance of cytokeratin19 (CK19) expression in diagnosing micrometastases in lymph nodes in patients with laryngeal carcinoma. METHODS: Forty cases of laryngeal carcinoma together with 163 lymph nodes were studied by the staining with hematoxylin and eosin(HE)and immunostaining with antibody against cytokeratin 19 (CK19) on histological sections of the primary tumor and regional lymph nodes. RESULTS: In all cases, CK19 was positively expressed in the primary tumor. Among 163 lymph nodes, metastases were confirmed by HE in 23 lymph nodes, and in 42 lymph nodes staining with immunohistochemistry, micrometastases were found in 19 lymph nodes. Micrometastases in the lymphonodes were significantly related to the T staging. CONCLUSION: Immunohistochemical staining is a valuable method for the detection of node micrometastases in patients with laryngeal carcinoma.

Objective To investigate the changes in atrial tissue and atrial myocytes as a result of chronic atrial fibrillation (AF) in humans. Methods Twenty patients with rheumatic heart disease were divided into chronic atrial fibrillation group (n=10) and non-fibrillation group (n=10). Tissue specimens harvested from the atrial appendage were studied with light and electron microscopy. Results The content of atrial connective tissue in the fibrillation group (26.7?7.2) was significantly higher than that in non-fibrillation group (12.4?5.9) (P