Objective:To investigate the efficacy of low-dose thyroid hormone treatment and the change in levels of thyroid hormone in patients with chronic kidney disease. Methods: 92 children with chronic kidney disease were randomly divided into thyroxine treatment group and control group, with 46 children in each group. All patients received conventional treatment, the treatment group served with additional Levothyroxine of 25 ~ 50?g/d. At least 1 month follow-up was taken to compare the clinical efficacy, RIA detection level of thyroxine. Results: In control group ,urinary protein, urine occult blood, Hb and albumin showed no changes at 1w-4w after therapy, and the treatment group also showed this trend. There were no difference between two groups. However, 1 week after treatment, SCr and BUN began to decline, till 4th week(P

Objective To investigate the relationship of the occurrence of sinusitis with nasal septum devia-tion.Methods 102 patients with nasal septum deviation confirmed by spiral CT and sinus endoscopy were included. The coronal CT images of all cases were observed.The incidence of sinusitis in different type of nasal septum deviation was calculated and the correlation between sinusitis with nasal septum deviation was analyzed.Results Among 102 patients with nasal septum deviation,deviated septum at high position in 71 cases,deviated septum beyond high position in 31 cases.Sinusitis was detected in 67 cases,accounted for 65.6%,of which unilateral involving in 38 cases,bilateral involving in 29 cases.Main lesions in the nasal septum deviation concave side in 46 cases (68.7%),in nasal septum convex side in 12 cases (17.9%).Conclusion Deviated septum is an important factor in onset.Upper septum deviation and deviated concave side more likely arise sinusitis.

Objective To analyze the use of narcotic drugs and the first class of psychotropic drugs in inpatient pharmacy of Wuhan Red Cross Hospital from 2011 to 2015.Methods Using the defined daily dose (DDD) which recommended by WHO as an index, and the dispensatory and clinical routine dose recommended by Chinese Pharmacopoeia and New Materia Medica as references.Adopting DUI as the statistic index, the drug's variety, quantity, sums and use frequency of the narcotic drugs and the first psychotropic drugs in inpatient pharmacy of our hospital from 2011 to 2015 were analyzed.Results According to the results, there was an increasing trend in sales amount from 2011 to 2015,among them,4 kinds of injections, 2 kinds of oral tablets,1 kind of transdermal patch and 1 kind of the sustained-release tablets.The sales amount of narcotic drugs showed an upward trend year by year.Conclusion In the past 5 years,the use of narcotic drugs and the first class of psychotropic drugs in inpatient pharmacy of the hospital are basically rreasonable, but it still needs to continuously strengthen the knowledge management of narcotic drugs and the related training, so as to ensure safe and effective use of medication in patients.

As hepatic fibrosis and cirrhosis have chronic progressive process,the assessment of fibrosis is of great significance for making appropriate treatment.It is divided into two categories,which includes invasive and noninvasive assessing methods.As a noninvasive method,magnetic resonance shows prospect in many aspects.This article is a review of magnetic resonance imaging in the assessment of hepatic fibrosis and cirrhosis.

OBJECTIVE： To develop a method of thin－ layer chromatographic scanning to determine the content of puerarin in Jiangtang pill METHODS： Silica GF254 thin－ layer was used， and the mobile phase consisted of chloroform－ methanol－ water(7∶ 2 5∶ 0 25) with the detection wave－ length of 254nm and the control wave－ length of 370nm RESULTS： The linear range of puerarin was 0 4～ 2 0μ g The mean recovery was 99 40％ (n=5) with RSD of 1 37％  CONCLUSION： This method is accurate， reliable and can be used for the determination of puerarin in Jiangtang pill

Objective To interpret genetic variation and population structure of Anopheles dirus A and D from China by molecular marker. Methods Samples included An. dirus A of Hainan laboratory colony (n=13), and field specimen from Mengla (n=17) and Jiangcheng (n=17) in Yunnan Province. The specimens were identified by PCR assay before study. mtDNA-COⅠ region was amplified and sequenced. Genetic variation and population structure was estimated according to sequence data. Results The mtDNA-COⅠ gene with a length of 959 bp was analyzed. There were three haplotypes in An. dirus A and six haplotypes in An. dirus D. The above haplotypes distributed in three populations unif-ormly. The average number of pairwise differences within Mengla population (7.441 2) was greater than that of Jiangcheng (1.279 4) and Hainan (1.051 3) populations, which suggested that the level of genetic divergence was the highest within Mengla population. The result of hierarchical AMOVA estimation showed a limited geneflow (Fst=0.799 9), therefore the variation level in a population (20.01%) was smaller than among the populations (79.99%). Conclusion The inter-specific genetic variation between An. dirus A and D in China was small and the level of divergence among individuals was high.

Objective To observe the changes of serum high-sensitivity C-reactive protein (hs-CRP) level before and after stent-assisted angioplasty in patients with intracranial and extracranial arteriestenosis. Methods The level of serum hs-CRP were determined in 25 patients treated with cerebral artery stenting before stent implantation, 3 days after stent implantation and during 6~12 months of follow-up. The serum levels of hs-CRP in non-restenosis group and restenosis group 6~12 months after stent implantation were also compared. Results The levels of serum hs-CRP 3 days after stent implantation and during 6~12 months of follow-up were higher than those before operation (all P0.05). The level of serum hs-CRP in restenosis group was higher than that in non-restenosis group during 6~12 months of follow-up (P

Objective To screen a cell line which stably suppresses DAPK expression and to observe the growth characters.Methods Four pairs of shRNA were designed,synthesized and inserted into the pDsRed1-N1-U6 vector.The recombinant plasmids were purified and transfected into PC12 cell.Meanwhile,a pDsRed1-N1-U6 vector was transfected as control.The cell clones were screened by G418,and the stable PC12 cell line was established.DAPK expression was detected by Western blot.MTT method and Flow Cytometry(FCM) assay were used to assess the growth characters of the cell line.Results The shRNAs were transfected into PC12 cell and the cell clones were successfully screened out.Of the four recombinant plasmids,the F2 was the best interfering shRNA.Beyond our expectation,the F1 recombinant plasmid had an enhanced effect on DAPK expression.Conclusion A stable PC12 cell line with stable inhibition of DAPK expression by was established using siRNA expression vectors.

Objective To better understand the pathogenicity and evolution of Yersinia pestis, we carried out the whole genome sequencing of human-avirulent Yersinia pestis strain 91001, which was isolated from a species of rodent-Microtus brandti. Methods We utilized “whole genome shotgun” approach to get the genome sequence of 91001. Based on the finished and annotated genome sequence of 91001, as well as the previously published genome sequences of CO92 and KIM, we performed detailed comparative genomics analysis on their chromosomes and plasmids. Results The genome of 91001 consisted of one chromosome and four plasmids (pPCP1, pCD1, pMT1 and pCRY). The pPCP1 plasmid of 9 609bp was almost identical with its counterparts from reference strains, which possessed 10 CDS. Plasmid pCD1 was found to be a plasmid of the type III secretory apparatus, and its length was 70 159bp. Although its CDS are quite similar to those of the reference plasmids, there were obvious rearrangements which produced certain differences in structure among them. Another plasmid was pMT1, a 106 642bp plasmid, which showed slightly different architecture compared with the reference ones. There was no mutation in virulent-related genes of pMT1 and pMT1 of 91001, which seemed to have retained more fragments of an ancestor plasmid. pCRY was a novel plasmid discovered in this work. It was 21 742bp long and harbored a group of gene encoding type IV secretory system. pCRY seemed to be able to replicate. The length of chromosome of 91001 was 4 595 065bp, and among its 4 037 predicted CDS (coding sequences), 141 were possibly pseudogenes. There were many IS in the chromosome. Due to the rearrangments mediated by IS, the structure of 91001 chromosome showed significant differences compared with CO92 and KIM. According to the results of comparative genomics analysis, we deduced the genetic mechanisms of nitrate reduction, glycerol fermentation, arabinose and milibiose utilization in 91001. Conclusion According to the analysis of plasmids structure, pseudogenes distribution, nitrate reduction negative mechanism, gene comparison and chromosome architecture, we conclude that 91001 and other strains isolated from Microtus brandti and Microtus fuscus evolved from ancestor Y. pestis and then developed into a different lineage. The deletion of large genome fragments from 91001 chromosome and pseuogenes might contribute to its unqiue pathogenicity and host-specificity.

Objective To establish reliable proteomics analysis models for Yersinia pestis and obtain the basic proteomics data of this pathogen. Methods The human-avirulent Y. pestis strain 91001 was cultured as an experimental strain, and the proteins of which were extracted and divided into three parts fractionally according to their solubility. Then three different methods (Shotgun-LC-MS-MS, 1D-LC-MS-MS and 2D-MS) were used to analyze the extracted proteins. The obtained data were compared with the theoretical protein database of strain 91001 so as to identify the expressed protein of Y.pestis strain 91001 in this study. Results 971 proteins were identified by shotgun-LC-MS-MS method, accounting for 23.4% of the predicted proteins of strain 91001 (971/4 143). 915 proteins were identified by 1D-LC-MS-MS method, accounting for 22.1% of the predicted proteins of strain 91001 (915/4 143). However, with 2D-MS only 5.62% of the predicted proteins (233/4 143) were identified. Altogether 1 193 proteins were identified when the results of the 3 methods added together, accounting for 28.7% of all the predicted CDS in 91001. Conclusion The kind and quantity of proteins identified by various proteomics methods differ from each other dramatically, therefore it is necessary to utilize multiple methods to get more reliable protein profiles of Y. pestis.