Objective To explore the application value of non coherent diffusion weighted imaging(IVIM) model in magnetic resonance body element for differentiating benign and malignant breast cancer.Methods Compar-ison of dynamic enhanced magnetic resonance (DCE -MRI)was conducted in 60 patients with breast occupying lesions,all patients were not treated with biopsy or needle biopsy before MRI scan.The lesions were obtained by oper-ation or biopsy after MRI scan,confirmed by pathology,39 benign lesions(benign group),28 malignant lesions(malig-nant group).IVIM-DWI parameters of two lesions group(D value,D* value and F value)were compared,analyzed IVIMDWI model parameters(D value,D* value and F value)diagnostic efficiency of diagnosis of benign and malig-nant breast lesions.Results Compared with two lesions groups,in the IVIM DWI model parameters,the D value of the malignant group was (0.95 ±0.19)×10 -3 s/mm2 ,which was significantly lower than (1.33 ±0.25)×10 -3 s/mm2 of the benign group (t =3.89,P <0.05),the F value of the malignant group was (8.97 ±2.01)%,which was significantly higher than (7.01 ±2.13)% of the benign group (t =3.93,P <0.05),there was no significant differ-ence in D* values between the two groups (P >0.05).IVIM DWI model in differential diagnosis of breast lesions had high diagnostic efficiency.Conclusion IVIMDWI model in the diagnosis of benign and malignant breast cancer is highly effective,and it has high clinical value.

Objective To establish a protein preparation process for growth differentiation factor 5(GDF5),and explore its role in cartilage injury through in vivo and in vitro experiments.Methods The gdf5 gene was ligated with the pCZN1 vector to construct the recombinant plasmid pCZN1-hGDF5,which was then transformed into E.coli BL21(DE3) pLysS host bacteria.Single colonies were picked to screen for high-expression strains.The strains were induced by IPTG,and the protein expression form was detected by SDS-PAGE.A method for inclusion body washing,denaturation and dilution renaturation was established,and the protein was purified by Ni affinity column chromatography and DEAE ion exchange column chromatography.ATDC5 cells were used as the detection cell line,and the biological activity of rhGDF5 protein in promoting proliferation was determined by MTT method.A model of cell injury induced by interleukin-1β(IL-1β) was established.The expression of cartilage-related genes and proteins was detected by RT-qPCR and Western blot,and the effect of rhGDF5protein on chondrocyte differentiation was analyzed by Alcian blue staining and alkaline phosphatase(ALP) activity determination.Poloxamer 407(P407) and 188(P188) thermo sensitive gels were prepared by cold dissolution method,and detected for their rheological properties by a rheometer.A male C57BL/6J mouse model of osteoarthritis(OA) induced by destabilization of medial meniscus(DMM) was established.The mice were administered by intra-articular injection once a week for four consecutive weeks.The mice were sacrificed and their knee joint tissues were collected.After decalcification,paraffin embedding and sectioning,they were stained with safranin O and fast green and detected by immunohistochemistry,and the OARSI score was determined.Results The results of double enzyme digestion(Nco Ⅰ/BamH Ⅰ) showed that the recombinant plasmid pCZN1-hGDF5 was correctly constructed.The protein expression level of the selected rhGDF5 high-expression strain accounted for 35% of the total protein.The expressed protein mainly existed in the form of inclusion bodies.After refolding and column chromatography,an rhGDF5 homodimer protein with a relative molecular mass of approximately 25 000and an electrophoretic purity of over 95% was obtained.The rhGDF5 protein exhibited a good proliferation-promoting effect on ATDC5 cells within the dose range of 25 to 800 ng/mL,and no cytotoxicity was observed at high doses.The rhGDF5protein could effectively promote the increase in chondrocyte number and proteoglycan secretion,and significantly inhibit the expression of ALP in ATDC5 cells.The results of RT-qPCR and Western blot demonstrated that compared with the IL-1βgroup,the administration of rhGDF5 protein could effectively reverse the inhibition of collagen type 2(COL-2) and SRY-box transcription factor 9(SOX9)(COL-2:t=4.899 and 9.799,respectively,each P <0.05;SOX9:t=4.950 and 10.73,respectively,each P <0.05),and significantly inhibit the expression of matrix metalloproteinase 13(MMP13)(t=6.500 and23.51,respectively,each P <0.05).The poloxamer thermosensitive hydrogel containing 22% P407 and 5% P188 with rhGDF5 protein prepared by cold dissolution method showed the gelation temperature of(30.06±0.16)℃ and the gelation time of 92.67 s.The results of the DMM mouse OA model experiment showed that compared with the control group(0 ng/mL),the injection of rhGDF5 protein-loaded thermo sensitive hydrogel into the joint cavity effectively repaired the damaged joint,with the high-dose group having the best repair effect,with an OARSI score of 1.The medium-dose group had an OARSI score of 1.67,while the low-dose group had no significant effect,with an OARSI score of 5.33.The immunohistochemical results also showed that compared with the control group,the treatment with rhGDF5 protein increased the distribution of COL-2.Conclusion A simple and efficient preparation process for protein renaturation,dimerization promotion and purification of rhGDF5 inclusion body was established,and its effective effect on cartilage was proved through in vivo and in vitro experiments,which provides technical support for further development of OA treatment products.

Objective To investigate the correlation between the expression of P-SAPK/JNK and neuronal apoptosis in the striatum during permanent middle cerebral artery occlusion (pMCAO) in rats.Methods Fifty-four male Sprague-Dawley rats were randomly divided into sham operation group and pMCAO 1-,3-,6-,12-,and 24-hour groups (n =9 in each group).Apoptotic neurons in the striatum during cerebral ischemia were detected by TUNEL assay,the nuclear translocation of P-SAPK/JNK in the striatum by immunohistochemical staining expressions of P-SAPK/JNK protein by Western blot.Results The numbers TUNEL- and P-SAPK/JNK-positive cells in the striatum at 1 hour after pMCAO increased significantly (P =0.000 1),and reached the peak at 6 hours.The numbers of TUNEL-positive cells decreased at 12 hours,however,it still higher than the sham operation group (P =0.000 2).Western blot analysis showed that the expression of P-SAPK/JNK after pMCAO increased significantly,and the time-course change was in accord with the result of immunohistochemical staining Neuronal apoptosis in the striatum was significantly positively correlated with the expression of P-SAPK/ JNK (r =0.984,P =0.000 4).Conclusions Cerebral ischemia may induce neuronal apoptosis in the striatum through the activation of P-SAPK/JNK.

Antirheumatic Agents ; administration & dosage ; therapeutic use ; Biological Products ; administration & dosage ; therapeutic use ; Drug Therapy, Combination ; Evidence-Based Medicine ; Glucocorticoids ; administration & dosage ; therapeutic use ; Humans ; Immunosuppressive Agents ; administration & dosage ; therapeutic use ; Lupus Erythematosus, Systemic ; drug therapy ; pathology ; Lupus Nephritis ; drug therapy ; pathology ; Severity of Illness Index

Objective To investigate the effect of artesunate on the expression of heat shock protein 47(Hsp47)in pulmonary fi-brosis in rats.Methods Thirty SD male rats were randomly divided into control group,model group and artesunate intervention group (intervention group).Rats in control group were intrachacheally instilled with saline (0.4 mL),while rats in other two groups 5 mg/kg bleomycin.Rats in both control group and model group were intraperitoneal injected with 1 mL saline per day sub-sequently.And the rats in intervented group were intraperitoneal injected with 10 mg/100 g artesunate per day.The body weight of day 0,day 14 and day 28 were recorded.All the rats were sacrificed on day 28.The pulmonary coefficient and the hydroxyproline content were observed.The expression of Hsp47mRNA and CollagenⅠmRNA were evaluated by RT-PCR.The Hsp47 expression was also detected by Western Blot.The pathological changes were analyzed by hamatoxylin-eosin (HE)stain and Masson stain.Re-sults (1)The body weight of control group grew much faster than other groups (P <0.05).(2)Pulmonary coefficient:model group (12.31±1.89)mg/g>intervention group (8.54±0.67)mg/g>control group(4.81±0.38)mg/g (P <0.05).(3)Ashcroft Scores:model group(5.70±1.09 )>control group (3.08±0.56),model group(5.70± 1.09)>intervention group (4.01 ± 1.25).There was no significant difference between control group and intervention group(P >0.05).(4)Hydroxyproline content:model group (620.33±66.16)μg/g>control group(379.00±35.51)μg/g,model group (620.33±66.16)μg/g >intervention group(429.00± 36.51)μg/g (P <0.05),there was no significant difference between control group and intervention group.(5)Hsp47mRNA expres-sion:model group and intervention group > control group (P <0.05),there was no difference between the first two groups(P >0.05).CollagenⅠmRNA:model group > intervention group>control group (P <0.05).(6)Western blot showed that Hsp47 ex-pression of model group was the highest in all three groups.Conclusion These results indicate that artesunate might inhibit pulmo-nary fibrosis and depress CollagenⅠproduction,and it′s probably a result of Hsp47 down regulation.

Objective:To observe the occlusal changes of patients with chronic periodontitis(CP)after initial periodontal therapy an-alysed by T-scan Ш occlusal analysis system.Methods:26 patients with CP were included.The patients were treated by initial perio-dontal therapy,including scaling,subgingival curettage,root planning and oral hygiene instruction.The occlusion time(OT),disoclu-sion time(DT),center of force trajectory(COFT),the percentage of bite force for maximum movie force at maximum area frame (MABF /MMF)and the bite force distribution balance between the left and right sides (BFDB)were measured by T-scan III occlusal analysis system before and six weeks after the treatment.All the data were statistically analyzed.Results:After initial periodontal treatment,the OT and DT extended for (0.20 ±0.14)s and (0.11 ±0.08)s respectively(P <0.05),the COFT increased by (0.81 ±1.19)mm(P <0.05).Moreover,the MABF /MMF and BFDB decreased by(7.20 ±4.41)% and (8.90 ±7.71)% respectively (P <0.05).Conclusion:Initial periodontal treatment may improve the occlusion of patients with chronic periodontitis.

Objective To evaluate the value of narrow band imaging(NBI) in the diagnosis of early squamous esophageal cancer and precancerous lesions.Methods Four thousand and fifty-six patients were examined by routine endoscopy,NBI and iodine chromoscopy,one hundred and fourteen lesions in 82 patients were screened.Of all lesions were detected by NBI with magnification and targeted biopsy.The differences among routine endoscopy,NBI and iodine chromoscopy,and the consistency between IPCL and histological findings were assessed.Results Compared to NBI and iodine chromoscopy,especially flat lesions,there was a high missed diagnosis rate in diagnosis of early squamous esophageal cancer and precancerous lesions by routine endoscopy.With NBI and iodine chromoscopy,the incidence rate of lesions was 78.0% and 79.8%,respectively.For iodine staining,85.7% Grade Ⅰ was high grade intraepithelial neoplasia(HGIN),and 66.0% Grade Ⅱ/Ⅲ was low grade intraepithelial neoplasia(LGIN).For NBI,80.4% Grade Ⅰ was HGIN,but there was no specificity in diagnosis LGIN.In appearance of IPCL,92.9% Type Ⅳ/Ⅴ was HGIN,89.4% Type Ⅱ/Ⅲ was LGIN,and it has a relatively better consistency in IPCL with histological findings.Conclusion There is a high detection rate in diagnosis of early squamous esophageal cancer and precancerous lesions by Lugoul's iodine staining and NBI endoscopy.It's probably that IPCL patterns by NBI with magnification can provide scientific basis for both the endoscopic theraphy of early esophageal cancer and the omen of postoperative recurrence.

Objective To investigate the effect of pioglitazone on oxazolone-induced colitis and to clarify the mechanism of apoptosis by Fas/Fas ligand(FasL). Methods Eighteen BALb/c mice of ulcerative colitis induced by oxazolone enema were equally allocated into 3 groups. The mice in group 1 were sacrificed 3 days after enema,lamina propria mononuclear cells(LPMC) were isolated from freshly obtained colonic specimens and treated in vitro with pioglitazone (40 ?mol/L),the annexin-V-FITC was used for detection of apoptosis,and Fas/FasL expression was assayed by flow cytometry. Meanwhile,untreated mice were served as normal controls. Mice in group 2 (control group) and 3 (experiment group) were treated with methylcellulose or pioglitazone(20 mg?kg -1 ?d -1 ) 3 days after enema and were administrated for consecutive 7 days. The colonic inflammation including disease activity index (DAI),macroscopic and histological changes,myeloperoxidase(MPO) activity and levels of interleukin (IL)-4,IL-5 were evaluated. Results Apoptosis of LPMC,expression of Fas and FasL in normal and colitis mucosa were 12.89?1.23,70.63?6.24,8.59?5.47 and 4.25?0.84,62.60?5.85,23.75?10.23,respectively. After treated with pioglitazone,both apoptosis of LPMC and expression of Fas increased,while FasL expression decreased (40.58?10.32,83.98?11.38 and 10.04?5.21 respectively). In group 2 and group 3,the macroscopic and microscopic score,MPO activity,IL-4 and IL-5 level were 2.50?0.55,8.83?0.75, 3.81?0.17,216.46?34.32,102.28?25.74 and 0.33 ?0.52,4.00?0.63,1.25?0.16,179.36?18.15,61.65?17.45,respectively. Conclusion The results indicate that pioglitazone treatment can significantly attenuate colonic inflammation,and it might be related to the apoptosis of LPMC through Fas and FasL.

0.05).While compared with the control,the permeation enhancement effects of 5% concentration of Lecithin,Oleic acid,Menthol,Limonene,and Azone were 1.37,2.25,3.71,6.75,and 10.15 times,respectively.CONCLUSION: Compared with Simomenine Hydrochloride,Simomenine free base had excellent transdermal characteristics,and was more suitable for the development of new transdermal preparation.