Objective To investigate the effects of different concentrations of glutnine(Gln) on the procfion of nitric oxide (NO)and reduction in mitochondrial membrane potential in rat heputocytesactivated in by interleukin(IL)-1β.Method The primary cultured rat hepatocytes with high-purity Wfffe isolated from 6-week-old male Sprague-Dawley raL, by flirt in situ circulatory collagenase peffusion method.After incubation for hours.hepatocytes were stimulated by saline,or IL-l(1 nmol/L)or IL-I(1 mnol/L)combined with gIne in concentrations rangiIfrom 2 to 10 mmol/L.The culture muln and hepatocytes were collected at 24 hours after stimulation.The concentrations of alanine aminotransferase(ALT)and NO in the lnllln were detected by biochemical methods.The mitochondrial membrane potential of the hepatocytes was detected with flow cytometry after incubated with fluorescem probe JC-1.Statistic package ofSPSS 11.5 was used for the data analysand significant differences between mean8 were evaluated byQ、4k analysis.Results The average concentration ofand NOinthe culturemedium afterIL-Istimulationwas 38.2U/L and 72.7tmaol/L,respectively,whichwere sis,cantly big,herthanthose ofcontrol group(7.4 U/L and41.7nol/L,respectively,P<0.01).1hemitochondrial membrane potential ofhepatocytes in IL-lgroup was much lowerthan that in control group(30.O%vs.62.8%.P<0.01).Gin inhibited NO production induced byⅡ,lp,Jeleasing and reduction in mitochondrial II brahetential ofcuhured rat hepatocytes in a dose-dependent HmIll.Conclusions Ghtamine,the most abundant free amino acid in the body,call attenuate hepatocye injury mechateel in vitro by pro-inflammatory cytokine mediated.nlis protective effectmay be associatedwiththeinhibition of NO production and thereby amelioration of mitochondrialfunction.

Cytokine-induced killer (CIK) cells therapy plays an important role in cancer adjuvant treatment.Researches show that the number of peripheral blood immunocytes will change if patients with cancer accept the treatment of CIK cells.The changes of T cell subsets,regulatory T cells are relatively obvious,which may be one of the standards that can evaluate the curative effect of CIK cells.

Objective:To evaluate the results of repairing furcation and root perforation using Mineral Trioxide Aggregate(MTA).Methods:Cases with furcation perforations were divided into two groups randomly,MTA and IRM(control group)methods were adopted in present study.Cases that had root perforations were treated with MTA.Pretreatment,immediate posttreatment,and 1year follow-up radiographs were evaluated in a double-blind manner to determine the presence or absence of any pathologic changes adjacent to the perforation site.Results:21 cases were involved.The healing rate of furcation perforation in MTA group was higher(80%)when compared with that in IRM group(75%).However,statistical analysis showed no significant difference in success rates between both groups(P=0.722).The healing rate of root perforation using MTA was 100%.Conclusion:MTA provides an effective seal of furcation /root perforations,and promises in improving the prognosis of perforated teeth.

Objective To investigate the effects of iodine on the pathogenesis of Graves′ disease (GD). Methods The thyroid epithelium cells (TEC) from GD patients and para adenoma normal tissue from thyroid adenoma patients wereculturedrespectivelywithdifferentNaⅠconcentrations from 0 to 1 mg/L. The HLA DR expression of TEC was detected with Western blot. Meanwhile, the morphological change of TEC was observed after treatment of NaⅠ by scanning electronmicroscope (SEM). Results (1) When the concentration of NaⅠ in culture medium was over 100 ?g/L, the amount of HLA DR expression of TEC increased in GD group (P

Intracerebral hemorrhage (ICH) is the second most common type of stroke,and its fatality is high.The baseline hematoma volume and hematoma growth are the predictive factors for the poor outcome of the patients.Previous studies have shown that surgical evacuation of hematoma can reduce the hematoma volume and improve the outcome.However,several recent randomized controlled trials of craniotomy hematoma evacuation for ICH and a Meta-analysis have shown that it is no more beneficial than conservative treatment.The previous evidence of minimally invasive evacuation of hematoma for the treatment of ICH has suggested that it has potential benefits for patients with ICH; however,further research is needed to confirm it.

ObjectiveTo investigate the risk factors for acute renal injury (AKI) in patients with ischemic stroke.MethodsPatients with ischemic stroke were enrolled retrospectively.The general clinical data, vascular risk factors, drug use, stroke etiological typing, stroke severity, and baseline biochemical indices were collected.They were divided into either an AKI group or a control group according to whether AKI occurred or not.Multivariable logistic regression analysis was used to analyze the independent risk factors for occurring AKI in patients with ischemic stroke.ResultsA total of 214 patients with ischemic stroke were enrolled, including 32 (14.95%) had AKI and 182 (85.05%) did not have AKI.The proportions of patients in heart failure (62.50% vs.41.21%;χ2=4.998, P=0.025), mannitol use (87.50% vs.43.96%;χ2=20.643, P<0.001), furosemide use (87.50% vs.43.96%;χ2=20.643, P<0.001), contrast agent use (37.50% vs.19.23%;χ2=5.300, P=0.021), and contrast dosage >200 ml (28.13% vs.9.89%;χ2=6.637, P=0.010), as well as NIHSS score (18.0±4.5)vs.8.0±3.2;t=15.249, P<0.001), diastolic blood pressure (89.98±9.1 mmHg vs.80.56±8.19 mmHg, 1 mmHg=0.133 kPa;t=5.898, P<0.001), fasting blood-glucose (10.54±4.31 mmol/L vs.6.32±1.32 mmol/L;t=5.898, P<0.001), blood urea nitrogen level (11.21±2.13 mmol/L vs.7.98±2.34 mmol/L;t=7.293, P<0.001), and arterial lactate concentration (3.98±0.12 mmol/L vs.0.91±0.25 mmol/L;t=68.003, P<0.001) in the AKI group were significantly higher than those in the non-AKI group.Multivariate logistic regression analysis showed that after adjusting various confounding factors, higher NIHSS score (odds ratio [OR] 1.910,95% confidence interval[CI] 1.517-6.012;P=0.024), higher diastolic pressure (OR 1.816, 95% CI 1.652-3.876;P=0.018), arterial lactate concentration (OR 1.553, 95% CI 1.256-1.763;P=0.019), mannitol use (OR 3.765, 95% CI 2.081-9.658;P=0.017), furosemide use (OR 5.329, 95% CI 3.085-8.763 P=0.010), contrast agent use (OR 2.097, 95% CI 1.364-2.456;P=0.031), and contrast dosage >200 ml (OR 3.294, 95% CI 1.464-2.786;P=0.021) were the independent risk factors for AKI in patients with acute ischemic stroke.ConclusionsThe NIHSS score, diastolic blood pressure, arterial lactate concentration,mannitol use, furosemide use, contrast agent use and contrast dosage >200 ml were associated with AKI in patients with ischemic stroke.

BACKGROUND:There is controversy on the treatment of old femoral neck fracture with hemiarthroplasty.
OBJECTIVE:To observe the clinical effect of cementless hemiarthroplasty in the treatment of old femoral neck fracture, and to compare with total hip arthroplasty.
METHODS:A retrospective analysis was performed on the clinical data of 23 old femoral neck fracture patients treated by artificial joint replacement from January 2009 to June 2010. Among the 23 patients, 11 cases were treated with cementless hemiarthroplasty, and 12 cases were treated with total hip arthroplasty. The time for off-bed activity, Harris score and the incidence of perioperative complications were compared between cementless total hip arthroplasty and hemiarthroplasty.
RESULTS AND CONCLUSION:Al the patients were fol owed-up for 12-18 months. The active straight leg raising angle, time for off-bed activity, incidence of early postoperative complications and Harris score at 1 week after treatment of the cementless hemiarthroplasty group were better than those of the total hip arthroplasty group;there were no significant differences in Harris score at 6 weeks, 3 and 6 months between two groups;the incidence of forward hip pain of the cementless hemiarthroplasty group was higher than that of the total hip arthroplasty group. So, we general y think that cementless hemiarthroplasty has better short-term effect in the treatment of old femoral neck fracture, but the long-term integrated efficacy needs to be further identified.

Using the WISC R to judge two groups childrens IQ and the structure of intelligence. The test group included 47 children studied in special educational primary school (24 girls, 23 boys, aged from 8-11 years). The control group included 50 normal children (25 girls, 25 boys, aged from 8-11 years). All children were required to perform CATEGORY TEST. The result showed that there were significant differences in IQ between two groups. The IQ in the test group ranged from 46 69. So they could be diagnosed as Mental Retardation (MR). 14 MR children were out of balance between VIQ and PIQ. One child's PIQ was over 2SD higer than his VIQ. But the intelligence structures were very similar in two groups. There were siginificant differences on Category Test between two groups, and the result of Category Test was highly related to IQ (r=0.65). This indicated that Category Test could be used to test the thinking development of children.

Calcifying epithelial odontogenic tumor (CEOT) is a rare benign epithelial tumor of odontogenic origin. CEOT is a benign but a locally infiltrative tumor. CEOT has two clinical variants: intraosseous (central) CEOT and extraosseous (peripheral) CEOT. The peripheral type is rare. In this paper, we report two cases of CEOT. The diagnoses of the cases were verified by histopathology. This study aims to explore the clinical and imaging appearances of CEOT and improve the understanding of the disease.
Humans ; Odontogenic Tumors ; Skin Neoplasms

Objective To investigate the cognition and care needs of the patients with acute stage fracture thus providing reference for the construction of intermediate care model. Methods The data of 504 patients with acute stage fracture from December 2014 to April 2015 were investigated through questionnaire, and analyzed. The issues included gender, age, marital status, cultural level, degree of understanding of intermediate care, care needs, etc. Results 63.29%(319/504) of the patients didn′t know intermediate care before they filled in the questionnaire,only 3.97%(20/504) of the patients understood the conception of intermediate care; different stage of age had different cognition towards intermediate care, the differences were statistically significant( χ2=148.771, P<0.01); different stage of age, sex, marital status, cultural level had different care needs, the differences were statistically significant (H/u=9.692-129.097, P<0.05). Conclusions At present, the majority of patients have no conception about intermediate care, so it is necessary to build the model of intermediate care. As nursing staff we should provide different help according to different type people, thus we can promote patients' physical and mental health, improve patient satisfaction to the greatest extent.