Xi Jinping,Secretary General has recently called on scientists mastering knowledge and proving in practice,to write papers in the motherland and in the realization of modernization.Up to the beginning of this century,ancestors and predecessors in Keshan disease prevention and control have made great achievements,including therapeutic methods,prevention of Keshan disease by supplementing selenium and compiling health standards,and these achievements have played an important technical supporting role in effectively controlling the epidemic of Keshan disease and guiding the practice of national wide control of Keshan disease.In the past 10 years,we have continued improving the technical norms to guide the work of national prevention and control of Keshan disease,and conducting translational research in response to the challenges of national Keshan disease prevention and control emerged in the new era.To realize the China dream of Keshan disease prevention and control,we must strive to achieve fully and sustainable elimination of Keshan disease,and to summarize the experiences in Keshan disease elimination for generalizing in other disease control and elimination,to continue to write the big article of the China dream of Keshan disease prevention and control.

BACKGROUND: Fms-like tyrosine kinase-3 (FLT3) gene is one of the receptors for growth factors in early hematogenesis. By binding to its ligand (FL), FLT3 regulates the proliferation and differentiation of hematopoietic stem/pregenitor cells via cell signal transduction pathway. FLT3 juxtamembrane internal tandem duplication (ITD)mutation is correlated with leukemia onset, development and act as an independent factor for poor prognosis.OBJECTIVE: To explore the relationship between Flt-3/ITD mutation and acute leukemia (AL), and analyze its clinical implication.DESIGN: A repetitive measurement.SETTING: Department of Biochemistry and Molecular Biology, Shenyang Medical College.PARTICIPANTS: Totally 147 samples of leukemia patients were diagnosed by morphological bone marrow biopsy in Shenyang Central Hospitals affiliated to Shenyang Medical College from January 1999 to October 2004. They were 95 males and 52 females with the mean age of 14.5 years old (5-23 years old). According to FAB standard, there were 62 patients with acute leukemia [including 33 cases of acute myelocytic leukemia (AMLs, M1, M2), 3 cases of acute myelo-monocytic leukemia (AMMOLs, M4), 18 cases of acute monocytic leukemia (AMOLs, M5), 8 cases of acute megakaryocytic leukemia (AMKLs, M7)], 43 patients with acute lymphoblastic leukemia (ALLs), 13 patietns with juvenile chronic myelocytic leukemia (JCMLs) and 29 patients with myelodysplastic syndrome (MDSs). Informed contents were obtained from all the participants.METHOD: Flt-3/ITD was detected with polymerase chain reaction (PCR) sequence in the 147 leukemia patients.MArN OUTCOME MEASURES: The clinical manifestations and prognosis were observed in the ALL patients with Flt-3/ITD;The Flt-3 gene expression, Flt-3/ITD positive rate, duplication region and length were detected in all kinds of leukemia.to Flt-3 gene products, including 3 cases of AMLs (M1, M2), 1 case of AMMOL (M4), and 1 case of AMOL (M5). DNA sequencing and blast alignment revealed that ITDs existed in all samples with unusual products within exon 11, in various region and length (33-72 bp). Expression of Flt-3 in different levels was found in 43 cases of ALL, 29 cases of Flt-3/ITD all died short after the diagnosis, and the mean life span of was only 10.8 months.

With regard to the nature of meridians, the authors have presented the hypothesis of dual reflex in 1977. This means that acupunture can invoke some effects not only through CNS (long reflex), but through the connection of peripheral nerve terminals to transmit messages (short reflex). In this experiment, rats were anaesthetized by rethane. The superficial peroneal nerve (SPN) and the deep peroneal nerve (DPN) were found out and cut off in order to break off their connection to CNS. With electrical stimulation of SPN, the action potential (AP) on the DPN was recorded at the same time. AP was induced only in a few cases (5.7%). But, after electroacupuncture was reapplied to Zusanli (ST-39) point for 30 rain, we stimulated SPN with electricity. This showed that APs were recorded on DPN in 17 among 27 rats (58.6%). Since some tissues around Zusanli point involve both SPN and DPN, the results suggest that messages are transmitted between nerve terminals under certain condition and that impluses on a nerve stem may stimulate another nerve terminal. This might be the essence of propagated sensation along the channels.

Alzheimer disease(AD) is the most common cau se of dementia today. Th e characteristic histopathologic changes include neurofibrillary tangles, neurit ic plaques, neuronal loss, and amyloid angiopathy. The noted Alzheimer symptom is the dysfunction of learning a nd memory. Potassium channels play a key role in it. A 113-pS tetraethylammoniu m-sensitive potassium channel was consistently absent from AD fibroblasts and o lfactory neuroblasts. Cp20, a memory-associated protein, amyloid precuror prote in and presenilin which are all tightly associated with genetic Alzheimer diseas e can regulate the activities of potassium channels. The changes of potassium ch annels subtype need further study. Potassium channels are maybe the important dr ug targets in the treatment of Alzheimer disease.

Objective To explore the therapeutical effect of somatostatin(SST) on the damaged bowel barrier of 7-day-old SD rats with hypoxic-ischemic encephalopathy(HIE). Methods Total 7-day-old SD rats were randomly divided into untreated control group(n=6), sham-operated group(n = 6), HIE model group(n = 8) and SST-treated group(n = 8). After 6 hours of operation, abdominal aorta blood of the rats was collected to measure the level of D-lactic acid, the ileum was taken for pathological analysis and immunohistochemistry staining of SP. Results The level of D-lactic acid in HIE model group was obviously increased[(10.30 ± 1.70)mg/L], and there were significant differences compared to untreated control group, sham-operated group as well as SST-treated group respectively(P < 0.05, respectively). The cillia of ileum became widen and shorten, and the number of it decreased. At the same time, in the stratum mucosa and the neurofibrillary tangle, the expression of SP was increased (average optical density: 12.67 ± 5.46), and there were significant differences compared to untreated control group, sham-operated group as well as SST-treated group raspectively (P < 0.05,respectively).Compared with HIE model group,the level of D-lactic aeid decreased obviously[(7.35 ± 1.55) mg/L] in SST-treated group,and there were no differences compared to untreated control group and sham-operated group respectively. The intestinal villi became thinner and higher in the SST-treated group, even the number increased. Besides that, the expression of SP decreased(average optical density: 0.73 ± 0.09), and there were no differences compared to untreated control group and sham-operated group respectively. Conclusion The therapeutical effect of SST on the bowel barrier damage of 7-day-old SD rats with HIE is clear.

Objective To explore CT and MRI appearances of intracranial germinoma.Methods 19 patients with intracranial germinoma confirmed histologically and radiotherapy were selected as the object of this study.CT and MR findings of germinoma were analysed retrospectively.Results Of 19 cases,the tumors were located at pineal region in 12,the basal ganglia and thalamus in 3 and the sellar in 4.Germinoma of pineal region showed low signal intensity on T1WI,iso or hyper-signal intensity on T2WI,isointensity or slightly hyperintensity on CT with definite margin,the pineal calculus were embedded frequently by tumors.After administration of contrast material,the tumors showed marked and uniformity enhancement.Thickness of pituitary stalk and absence of the hyperintensity of the posterior pituitary on T1WI were found in sellar germinoma.Tumors showed iso-signal intensity on T1WI,but variable signal intensity was exhibited on T2WI.Calcification was absent in all cases,but the solid components of tumors were hyperdense on plain CT,and they were obvious enhancement on contrast-enhanced CT scan.Germinoma located at basal ganglia and thalamus showed diffuse growth,larger volum of tumors,inhomogeneous signal intensity and density with indefinite margin,hemorrhage seen in most of case.After administration of contrast material,the tumors showed patch or chaplet-like enhancement.Conclusion It is possible to make the preoperative diagnosis for intracranial germinoma according to sex,age and CT,MR imaging characteristics.

Sodium salicylate-borate is a complex salt easily prepared by the reaction of salicylic acid and borax at 40-50℃. A three-color point with Rf values of 0.72, 0.58, and 0.48 respectively was obtained on the thin layer chromatography. The maxium absorbent peak of this complex salt (20?g/ml) was at 300 nm. determined with the ultraviolet spectrophotometer scanner.The mycostatic effect of this complex salt varies with its concentrations. It is effective against the superficial infections with the concentration of over 1250 ?g/ml; and 2500?g/ml is necessary for the treatment of deep fungus infections.

Objective To provide patients and medical personnel with quality, highly effcient, and reasonably priced logistical services by forming specialized entities of logistical services and following the path of specialization, intensity and market mechanisms. Methods The principles of overall planning, phase-by-phase implementation and steady promotion were adhered to and the functions of logistical management and services were separated in accordance with the ideas of separating management and operation and the goals of outsourcing logistical services. Results The traditional models of "being small but complete" and "a hospital setting up a society" were transformed and new models of logistical management and services were established. Conclusion There has emerged a new pattern of outsourcing logistical services, with both sides sharing resources and complementing each other's advantages.

OBJECTIVE:Inhaled glucocorticoids(GC) are the first-line medicine for bronchial asthma during relieve phase, thereby this study was designed to explore if patient tends to suffer osteoporosis due to long term inhalation of glucocorticoids. DATA SOURCES:Literatures about GC and osteoporosis were computer-searched in Medline and Embase from January 1980 to December 2003, with the key word of " asthma, bone density, bone metabolism and inhaled glucocorticoids" and language limited to English.Meanwhile it was also computer-searched in CBM, CBM disc and manually searched in Chinese Journal of Clinical Rehabilitation between January 1990 and April 2004 with language limited to Chinese. DATA SELECTION: Literatures about the comparison of bone density and bone metabolic change between inhaled GC group and control group were consorted deleting randomized controlled trial(RCT) so as to look up the whole content of healthy controls;While patients in the treatment group received GC inhalation.Exclusive standards:obvious non-RCT. Quality assessment is mainly focused on the reality of data,the strictness of design and implementation,and the rationality of statistical management. DATA EXTRACTION:Totally 30 randomized and nonrandomized studies about the influence of inhaled GC on the bone density and bone metabolism of patients with asthma were referred to amongst which 18 studies were admitted with the other 12 excluded for similarity in content in 8 and Meta-analysis in 4. DATA SYNTHESIS:A total of 1 153 asthmatic patients in 18 studies were divided into GC inhalation group and non-inhalation control group for comparing the difference of bone density and/or bone metabolism between them. CONCLUSION:Although the results of studies on the influence of long-term inhaled GC on bone quantity were different, but there was still not enough evidence to demonstrate that osteoporosis was due to long-term GC inhalation.

Today,more and more patients with diabetes are requiring surgical intervention.Not only the metabolic disturbance of carbohydrate will be aggravated by surgical stress,but also the risk of perioperative complications and mortality have been greatly increased in patients with diabetes.Therefore,a complete preoperative assessment and blood glucose management of preoperative,intraoperative,and postoperative periods are needed to get full assurance for diabetic patients to undergo surgery and to pass through the perioperative period smoothly.