1.Pathological development of gastric mucosa in Helicobacter pylori related diseases
Tianshu LIU ; Jiyao WANG ; Shiyao CHEN
Chinese Journal of Digestion 2001;0(01):-
Objective To study relationship of Helicobacter pylori (H.pylori) eradication and pathological development of gastric mucosa among H.pylori related diseases. Methods One hundred and ninty one H.pylori infected patients were randomly given anti H.pylori or non anti H.pylori medications, and endoscopic examination was repeated one year after the treatment. Pathological classification was followed the Sydney system. Results Among 191 patients, degree of chronic inflammation of gastric mucosa had ameliorated ( P
2.Latent problems and thinking of the standardization of syndrome differentiation based on the diseases of Western Medicine(WM)
Maoxin LIANG ; Xuefeng WANG ; Tianshu GAO
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(03):-
The standardization of syndrome differentiation based on the diseases of WM has become an important academic guidance,which,however,has induced lots of problems.The logical contradiction was analyzed between the results of standardization of syndrome differentiation based on the diseases of TCM and WM,between the results of two large-scale standardizations of syndrome differentiation based on WM and between the results of syndrome differentiation of related diseases of WM.In this paper,the author studied on the origin of symptoms and signs of syndrome differentiation of the diseases of WM and the effect of standardization of syndrome differentiation of the diseases of WM on the nature and location of disease in TCM,and pointed out some important unresolved academic problems.
3.The expression of survivin gene in gastric cancerous tissue and its relationship with gastric cancer prognosis
Shiyao CHEN ; Quanhong WANG ; Tianshu LIU
Chinese Journal of Digestion 1996;0(05):-
Objective To explore the expression of survivin gene in gastric cancerous tissue and its relationship with gastric cancer prognosis. Methods All the samples were obtained from patients with gastric cancer who underwent surgery in Zhongshan Hospital from July 1995 to June 1996.“ Envision two steps”of immunochemistry was used to detect survivin expression in the resected gastric cancerous tissue.TNM stages were determined by pathological diagnosis and clinical status.All the cases were followed up at least 5 year or until death.The discrepancy of survivin expression was compared in different pathological types and TNM stages.Survivial curves were compared in the two groups with or without survivin expression. Results Totally 96 cases(male/female:59/37;age:29-84 years old,mean age 56) were recruited and survivin expression rate was 70.8%(68/96).Among pathological types,78 cases were adenocarcinoma,18 were non-adenocarcinoma.Survivin expression was similar in different pathological types (69.7% in adenocarcinoma vs.60.0% in non-adenocarcinoma,P=0.369).Among adenocarcinoma,survivin expression rate was higher in poorly differentiated group than that in well differentiated one(82.1% vs. 62.5%,P=0.053).As the infiltration denoted in adenocarcinoma,the expression rates of survivin were 33.3% in cases limited to mucosa and submucosa,81.3% in cases limited to muscularis layer and 75.9% in cases infiltrated to whole layers (P= 0.020).Expression rate of survivin was not related with lymph node metastasis(68.1% vs.70.8%,P= 0.771).Five-year survival rate in 68 survivin positive cases was 42.93%,lower than 52.93% of 28 survivin negative cases,but no statistical difference was observed. Conclusions Survivin is highly expressed in gastric cancer.Survivin expression is closely related with differentiation status of adenocarcinoma and degree of infiltration.Further studies are needed to evaluate its role in the prognosis of gastric cancer.
4.Optimize the extraction process of Qingrejiedu oral liquid by multi-index comprehensive evaluation
Tianshu GAO ; Huili WANG ; Yihong ZHAO
Chinese Journal of Biochemical Pharmaceutics 2015;37(7):141-144
Objective To optimize the extraction techique for Qingrejiedu oral liquid.Methods The amount of water, extraction time , extraction times and alcohol content were used as inspection factor, 3 levels per factor, with baicalin, chlorogenic acid , geniposide transfer rate and extraction rate as indexes,carries on Weighted comprehensive evaluation,the optimal extractin were screened by L9 (34 ) orthogonal test.ResuIts The optimal extraction condition of Qingrejiedu oral liquid were as follow:extraction for 3 times and 1h each time with 8-fold amount of water and 70% alcohol. ConcIusion Through the orthogonal test, the extraction process of Qingrejiedu oral liquid is designed reasonably, the result is reliable, and the extraction effect is good, and has higher application value.
5.Cardiac troponin I expression in human umbilical cord mesenchymal stem cells after 5-azacytidine induction in vitro
Xin TANG ; Nini WANG ; Haibo YI ; Yan WANG ; Tianshu PANG
Chinese Journal of Tissue Engineering Research 2013;(23):4212-4215
10.3969/j.issn.2095-4344.2013.23.005
6.Expression of specific genes of cardiomyocytes differentiated from human umbilical cord mesenchymal stem cells
Xin TANG ; Yan WANG ; Haibo YI ; Tianshu PANG
Chinese Journal of Tissue Engineering Research 2013;(27):4988-4991
BACKGROUND: Intramyocardial transplantation of autologous umbilical cord-derived mesenchymal stem cells for repair of myocardial tissue damage is paid increasing attention in the cardiovascular field. OBJECTIVE: Human umbilical cord mesenchymal stem cells were isolated and cultured. Passage 2 human umbilical cord mesenchymal stem cells were treated with various concentratins of 5-azacytidine (2.5, 5, 10, 20, 40, 80 μmol/L) for 24 hours . After removal of 5-azacytidine, cells were cultured for another 4 weeks. RESULTS AND CONCLUSION: Before 5-azacytidine treatment, filament-like structures or particles were not observed in the cells, but the amount of cytoplasm was less and uniform, nuclear/cytoplasm ratio was high, cells exhibited typical fusiform appearance and grew in a swirl-like manner, and nucleolus was obvious. After treatment with 5-azacytidine for 24 hours, some cells died in each group, and typical fusiform appearance turned into stick-like or column-like appearance, especial y in the 40 and 80 μmol/L 5-azacytidine groups. Reverse transcription-PCR results showed that atrial natriuretic peptide and α-skeletal actin gene expression levels were detected on human umbilical cord mesenchymal stem cells after treatment with 2.5 or 40 μmol/L 5-azacytidine for 4 weeks or with 5, 10, 20 μmol/L 5-azacytidine for 1, 2, 3 and 4 weeks. These findings suggest that 5-azacytidine-induced human umbilical cord mesenchymal stem cells express the specific gene of myocardiocytes.
7.Experience of nursing scheduling in the Ebola treatment center observation ward
Lihui WANG ; Ning LI ; Deli ZOU ; Tianshu LU
Chinese Journal of Practical Nursing 2016;32(4):255-257
Objective To investigate the characteristics of the diagnosis and treatment center for Ebola observing ward and reduce infection risk according to allocation scheduling method of nursing staff. To provide reference for other similar tasks. Methods We used the method of grouping fixed group scheduling method and fixed working hours to identify and refine the content of nursing work. Results A total of 65 cases of suspected Ebola including 5 cases diagnosed as EVD patients were cured. No case of infection occurred in nursing staff. Conclusions During the infectious disease nursing work, reasonable personnel allocation and scheduling method can make the nursing staff work with full energy and strength and ensure the realization ofzero infectiontargets.
8.Attenuated and Synergistic Effect of Meteorism Tablets in Treatment of HBeAg-positive Chronic Hepatitis B
Huili WANG ; Tianshu GAO ; Xiaoping YANG ; Yuyao ZHAO
Chinese Journal of Information on Traditional Chinese Medicine 2013;(11):8-10
Objective To observe the attenuated and synergistic effect of meteorism tablets in treating HBeAg-positive chronic hepatitis B patients. Methods Eighty patients of HBeAg-positive chronic hepatitis B were randomly divided into treatment group (meteorism tablets combined with telbivudine) and control group (telbivudine), and treated for one year. The changes of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), the negative rate of HBV-DNA, the negative rate of HBeAg, HBeAg/HbeAb serological conversion rate, creatine kinase (CK) before and after treatment, and safety were observed. Results After treatment, biochemical response, virological response and HBeAg serological response was more significant in the treatment group than that in the control group (P<0.05). The percetage of patients with elevated CK in the treatment group was significantly lower than that in the control group (32.5% vs 60%, P<0.05). Conclusion Meteorism tablets combined with telbivudine has attenuated and synergistic effect in treating HBeAg-positive chronic hepatitis B patients.
9.Significance and implication of the changes of serum interlukin-11 level in patients with hematological malignancy post-chemotherapy
Kaitai WANG ; Hongjuan LU ; Tianshu WANG ; Jun SHEN ; Haiyan YUAN ; Lili ZHOU ; Xiaofen YANG
Journal of Leukemia & Lymphoma 2009;18(5):287-289
Objective To explore the relationship between plasma interleukin-11(sIL-11) level and platelet count post-chemotherapy with hematological malignancy patients and analyse a sIL-11 level which may maintain a safe platelet count so as to guide the treatment. Methods Blood samples were collected from the patients with hematological malignancy at certain time point of pre-and post-chemotherapy, and serum level of sIL-11 and platelet count were determined separately. Different statistical methods were applied to test the relationship between sIL-11 level and platelet changes. Results 99 cases finished this study. The findings are: the sIL-11 level went up and reached the peak on day 6 post-chemotherapy, while the platelet count kept dropping to the lowest on day 10, the sIL-11 peak occurred before the lowest platelet count, patients with faster sIL-11 increase may maintain a comparatively higher plateled count. 99 eases were grouped according to the lowest platelet count and compared: the group with higher platelet count tend to have higher peak sIL-11, more cases with higher peak sIL-11, with faster daily average sIL-11 increase, the lowest platelet count occurred later. Logistic regression analysis showed the factors contributed to lower platelet includes slower daily average sIL-11 increase and sIL-11 level less than 2000 pg/ml on Day4 post-chemotherapy. Conclusion There were correlation between the serum sIL-11 level and platelet counts, the platelet count change may be predicted by determining the plasma sIL-11 level post-chemotherapy. Patients with sIL-11 level less than 2000 pg/ml on Day4 post-chemotherapy may be endangered with severe thrombocytopenia, rhIL-11 or platelet transfusion treatment should be considered.
10.Current Situation and Prospect of Chinese Medicine Pharmacology on Autoimmune Thyroiditis
Xiao YANG ; Guoxin LI ; Zhimin WANG ; Xiyu ZHOU ; Jinqiao QU ; Tianshu GAO
World Science and Technology-Modernization of Traditional Chinese Medicine 2017;19(5):865-869
As the most common organ-specific autoimmune disease,autoimmune thyroiditis (AIT) is characterized by intrathyroidal lymphocyte infiltration and specificthyroid autoantibodies.Modern medicine is short of effective etiological treatment at present,and has no specific medicine foreuthyroidism of AIT patients,who have been followed-up passively.Chinese medicine treatmenthas the characteristics of multiple-targets,multiple-links and multiple-pathways,which plays a special superiorityrole in the prevention and treatment of AIT.The purpose of this article was to sort out and set forthmore Chinese medicine pharmacology on AIT recently,which provided evidence for further clinical apply.