Epilepsy is a common chronic brain dysfunction in children.Studies have showed that antiepileptic drugs (AED) affect the hydroxylation of vitamin D in liver and kidney and inhibit the calcification of bone,mainly by up-regulating cytochrome oxidase P450 and promoting the resolution of vitamin D,and then increasing serum parathyroid hormone compensatory.Long-term use can cause children metabolic bone disease occurrence,clinical manifestations include bone pain,short stature,rickets,teeth agenesis,fracture,etc.AED firstly affect the hormones related to bone metabolism in vivo,so that the present researches focus on biochemical tests and measurement of sclerotin and bone density.In the children's long-term use of antiepileptic drug treatment at the same time,the application of Vitamin D,calcium and exercise can prevent the occurrence of metabolic bone disease.

Objective To study the level of brain natriuretic peptide of acute myocardial infarction(AMI)patients given percutaneous coronary intervention(PCI).Methods 200 patients with AMI were collected and randomly divided into observation group given PCI and control group given thrombolytic therapy.Then level of BNP 6h,12h,24h and 48h after treatment was detected and cardiac function indexes were observed.Results BNP level of observation group began to reduce from 6h(t=5.382,9.493,12.482,all P＜0.05),control group began to reduce from 12h(t=7.831,12.184,all P＜0.05);ST segment elevation of BNP＜100ng/L group was significantly lower than that of ≥ 100ng/L group(t=9.472,P＜0.05);LVEDD,LVEF were significantly higher than those of ≥ 100ng/L group(t=6.932,5.532,all P＜0.05).Conclusion PCI can decrease level of BNP and improve heart function in a relatively short time and has positive therapeutic significance.

Acute lymphoblastic leukemia (ALL) is a malignant clonal disease deriving from B or T lineage lymphoid precursors.ALL characterized by its high malignancy,poor curative effect and low remission rate occurs most frequently in pediatrics,but also accounts for 20 ％ of leukemia in adult.The further study of the biological feature and pharmacology may play a significant role in the improvement of the survival rate and life quality for ALL patients.The advent of continuous human ALL cell lines provides irreplacable cell-model for the occurrence,evolvement mechanism and drugs research in ALL.Besides,ALL cell lines established at present have covered most of the immunophenotyping and specificity chromosome abnormality,which promote the progress of genetics diagnosis and target therapy.This paper will review the advances in ALL cell lines,especially the cell lines with Ph chromosome and MLL gene alterations.

Long non-coding RNAs (lncRNA) are a class of non-coding RNAs (ncRNA) that contain over 200 nucleotides.Asthma is one of the most common diseases in children and the main characteristics including airway inflammation,airway hyperresponsiveness and airway remodeling.Studies have shown that some lncRNAs participate in the identification of steroid-resistant asthma and have an important role in the treatment of glucocorticoid hormone,while some lncRNAs have a link with microRNAs (miRNA),inflammation,epigenetic modification and airway smooth muscle cells,lncRNAs maybe closely related to the development of asthma.This review will discuss the association between lncRNAs and asthma.

Objective:To establish normal Delaire Architectural Analysis database of the Han nationality adults with normal occlusion in Chongqing.and to explore their cranio-maxillo-facial structure features.Methods:Seventy-eight Han adults with normal occlusion(male 37,femal 41)aged from 18 to 26 years were included.All adults were with normal and well-balanced faces,did not receive orthodontic or orthonathic treanment.The lateral cephalogram of each normal adults were traced,digitized, measured by Winceph8.0 software.Statistical calculation performed cephalometric values was established,compared with their craniofacial structure characteristics with Korean's.Conclusion:To establish the normal database of cranio-maxillo-facial structure of Chongqing adults with Delaire architectural analysis.

Necrotizing enterocolitis (NEC) is one of the common critical disease in preterm infants,not only the mortality is high,but part of the surviviors suffer from complications,such as intestinal stenosis,intestinal obstruction,malnutrition,parenteral nutrition associated cholestasis,and even short bowel syndrome,intestinal function failure and neurological dysfunction.The etiology of NEC is complex and the pathogenesis has not yet entirely clear.Therefore,early diagnosis of NEC is more difficult.In recent years,premature birth,unreasonable feeding,Toll-like receptors expression,bacterial colonization,hypoxia ischemia and hemodynamic changes are considered to be the risk factors of NEC.However,breast feeding and probiotics can reduce the incidence of NEC in premature infants.

To investigate the clinical efficacy of cyclocryotherapy combined trabeculectomy and single cyclocryotherapy for neovascular glaucoma (NVG).
●METHODS:Totally 82 patients with NVG from February 2012 to June 2013 in our hospital were selected and randomly divided into observation group and control group, each 41 cases. Patients in control group were given single cyclocrytherapy, and patients in observation group were given cyclocryotherapy combined trabeculectomy. The visual acuity, intraocular pressure and postoperative complications of two groups were observed.
●RESULTS: After postoperative six months, the improvement rate of visual acuity in observation group was 61. 0%, which was significantly higher than that of control group ( 41. 5%), the difference was statistically significant (χ2 = 3. 843, P = 0. 018). lntraocular pressure of observation group was significantly lower than that of control group, the difference was statistically significant (P<0. 05). The complication rates ( eye pain, hyphema, and corneal edema) of observation group were 9. 8%, 12. 2%, and 9. 8%, which were significantly lower than that of control group (51. 2%, 60. 9%, and 48. 8%), the difference was statistically significant (χ2 = 7. 594,P= 0. 007;χ2 = 9. 276,P= 0. 000;χ2 = 6. 835,P = 0. 013). The difference of two groups was not statistically significant on complications of conjunctival edema, anterior chamber exudation, shallow anterior chamber, and eyeball atrophy (P>0. 05).
●CONCLUSlON: Compared with single cyclocryotherapy, cyclocryotherapy combined trabeculectomy has advantages of low intraocular pressure, less postoperative complications, and high success rate, so it is worthy of promotion.

For the research of peripheral nerve injury, peripheral nerve were injuried with crushing, cutting or pulling and so on. The way of injury should meet the needs of the clinical practice. This article summarized a list of the literature of related animal experiment of peripheral nerve injury.

AIM To investigate the effects of the novel compound pivanampeta on the fatty liver induced by cholesterol-feeding in rabbits and quails. METHODS ①24 male rabbits were divided into four groups randomly as following: control group, model group, pivanampet 1 and 5 mg?kg -1 groups. The serum levels of total cholesterol were determined after the rabbits were cholesterol-fed for 12 weeks. The morphological changes of liver were observed. The levels of cholesterol and malonal-dialdehyde and the activities of glutatione peroxidase in the homogenate of liver were also measured.②181 male quails were divided into six groups randomly:control group, model group, Simvastatin 5 mg?kg -1 group, pivanampet 3,6 and 9 mg?kg -1 groups. The serum levels of total cholesterol were determined after the quails had been fed with cholesterol diets for 11 weeks. After 14 weeks part of the animals were killed, the morphological changes of liver were observed. The hepatic coefficient were determined after the remaining quails were cholesterol-feeding for 18 weeks. RESULTS Pivanampet alleviated the liver Steatosis induced by cholesterol-feeding in rabbits and quals. It decreased total cholesterol levels, elevated the activity of glutatione peroxidase in the rabbits liver, and reversed the increasing hepatic coefficient in rabbits and quails. CONCLISION The novel compound Pivanampet can delay the formation of fatty liver by cholesterol-feeding.

Objective: To study the effect of esomeprazole in treating elderly patients with NSAIDs correlated peptic ulcer bleeding. Methods: Patients with NSAIDs correlated peptic ulcer bleeding in our hospital from January, 2010-December, 2012 were enrolled and randomly divided into observation group given esomeprazole and control group given omeprazole. Then the baseline data were surveyed and effect of ulcer bleeding, condition of abdominal pain alleviation and ulcer ulcer, adverse reaction cases were observed. Results: (1)there were no significant difference between the baseline data of two group;(P>0.05);(2)in observation group, excellence rate was 42/70(60%), effective rate was 24/70(34.3%), the overall treatment effect was better than that of control group, the difference had statistical significant(P<0.05);(3)there were no significant difference between two group of abdominal pain and ulcer healing;(4) during treatment, there were no serious adverse reactions in the two groups. Conclusion:Compared with omeprazole, esomeprazole has equal effect on abdominal pain relief and ulcer healing, and it is much more effective in treating elderly patients with NSAIDs correlated peptic ulcer bleeding.