At present,research of genes and signal transduction pathway for etiology and pathogenesy of diseases is a hot topic.STAT3 signal transduction plays an important role in regulating cell proliferation,differentiation,survival and apoptosis.As a point of convergence for numerous oneogenie signaling pathways,STAT3 is constitutively activated in diverse human cancer cells.Current study suggest that activation of STAT3 signal transduction is closely related to the currence,development and malignant biological behaviors of tumor.STAT3 has been identified as a promising target for cancer treatment.

Books ; Medicine, Chinese Traditional ; Translations

Purpose]The purpose of this thesis is to briefly study how to translate different types of anadiplosis in Yellow Emperor's Canon of Internal Medicine, so as to evoke other translators'attention in this field. [Methods] There are different types of anadiplosis in Yellow Emperor's Canon of Internal Medicine, thus this thesis first summarizes the categories of anadiplosis structure in Yellow Emperor's Canon of Internal Medicine, then studies the respective use and mixed use of domestication and foreignization strategy in translation of the three types of anadiplosis structures. Furthermore, it also analyzes the advantage and disadvantage of domestication and foreignization strategy and the scope of their application. [Results] Through research, the thesis discovered that both domestication and foreignization strategy have their own advantage and disadvantage in translating anadiplosis in Yellow Emperor's Canon of Internal Medicine. On one aspect, translators should spread as much traditional medical culture as possible; on the other hand, translators should also take readers' response into accounts. Although domestication is a major trend nowadays, with the deepening of culture exchange, foreignization will play a more important role in TCM classics translation. [Conclusion] Domestication and foreignization strategy complement each other in the translation of anadiplosis in the Yellow Emperor's Canon of Internal Medicine. However, because of the requirement to spread traditional Chinese medicine, foreignization strategy will be used more frequently than before.

Objective To detect supernatant of SD rat bone marrow mesenchymal stem cells(BMSC)and liver cells,and explore the role of cytokines,and provide a theoretical basis for the biological artificial liver and clinical treatment of liver failure.Methods BMSCs and primary hepatocytes of SD rat were isolated.BMSCs with its third generation and liver cells by the ratio of 1∶10 were co-cultured.Hepatocytes and BMSC were treated as control group.Observation the cells′survival and morphology and analysis of cytokine after 48 h were made by RayBiotech.Results BMSC-hepatocyte in the co-culture group grow and proliferated rapidly,and hepatocyte could survive up to 14 days;while hepatocyte cultured alone,it grow slowly and survived only 9 days.Expression of BM-SC-liver cells supernatant changed obviously:interleukin 1,interleukin 6,interleukin 10 were higher than those in the control group,above 2 times,which showed significant difference (P<0.01).Conclusion Co-culture of BMSC-liver cell could induce BM-SC secrete paracrine and autocrine:IL-1 and IL-6 and IL-10,which could promote the growth of hepatocyte and extend the liver cell survival time.In-vitro BMSC-liver cells could provide biological artificial liver cell sources,as well as provide theory basis for using cytokine treatment for liver failure.

Objective To research the effect of GANT61 on epithelial‐mesenchymal transition (EMT) in human lung cancer H1703 and A549 cells lines ,and to preliminarily investigate its action mechanism .Methods DMSO was used as the control(DMSO group) .After treating H1703 and A549 cells with GANT61 for 24 h ,the gene changes of Gli‐1 ,Gli‐2 ,E‐cadherin and Vimentin were detected by using the real time fluorescence quantitative PCR method .The influence of GANT61 on the expression of E‐cad‐herin and Vimentin protein after acting on H1703 and A549 cells was observed by using the Western blotting assay .The scratch healing test was performed to evaluate the effect of GANT 61 on the tumor cell invasion ability after acting on H1703 and A549 cells .Results The real time fluorescence quantitative PCR showed that ,compared with the DMSO group ,GANT61 down‐regulated the mRNA expression of Gli‐1 ,Gli‐2 and Vimentin mRNA of H1703 and A549 cell lines and elevated the expression of E‐cadherin protein(P<0 .01);the Western blotting showed that GANT61 down‐regulated the expression of Vimentin of H1703 and A549 cell lines ,and elevated the expression of E‐cadherin(P<0 .01);the scratch healing test revealed the invasion ability of H 1703 and A549 cells in the GANT61 treatment group was significantly decreased (P<0 .01) .Conclusion EMT in lung cancer is related with aber‐rant activations of Gli1 and Gli2 in Hedgehog signal transduction pathway ,GANT61 could influence the EMT ability in lung cancer cells by down‐regulating the expression of Gli‐1 and Gli‐2 .Gli could become a new molecular target for inhibiting the lung cancer cell metastasis.

ObjectiveTo explore the relationship between β2-adrenergic receptor (β2-AR) gene Arg16Gly polymorphism and essential hypertension (EH) in elder (≥60 years old).Methods150 old healthy controls and 115 old patients with EH (all of them without kin relation) were selected to test genotype of β2-AR by the technique of polymerase chain reaction-restriction fragment length polymorphism (PCR-PFLP).ResultsThe frequencies of three genotypes (Arg/Arg, Arg/Gly and Gly/Gly) were 22.81%, 53.51% and 23.68% in the EH group and 27.33%, 67.33% and 5.33% in the controls respectively. The frequencies of Arg and Gly allele were 49.56 % and 50.44% in the EH group, and 61.00% and 39.00% in the controls. There was a significant difference in distribution of alleles/genotypes between EH group and controls ( P<0.05).Conclusionβ2-AR Arg16Gly gene polymorphism is possibly associated with hypertension in this study population.

AIM:To observe the effects of cornus iridoid glycosides(CIG)on inflammatory reaction especially the inflammatory cytokines in the brain after traumatic brain injury,and to explore the possible mechanisms of its neuroprotective effect.METHODS:SD rats were intragastrically administered with different doses of CIG(30,60 and 120 mg?kg-1?d-1)for 7 d.The traumatic brain injury rat model was induced by improved Feeney's fall weight method,and the brains were taken out 24 h and 72 h after brain injury,respectively.The morphological changes were observed by HE staining in the cerebral cortex.The expressions of inflammatory cytokine tumor necrosis factor-?(TNF-?)and interleukin-1?(IL-1?)were detected by immunohistochemical method.The image processing and statistical analysis were used to measure the number and the area of immunoreactive cells.RESULTS:HE staining showed the pathological changes were serious in the cerebral cortex of model group,and compared with the model group,the pathological changes were obviously reduced in CIG group.The positive immunoreactive cells of TNF-? and IL-1? were mainly distributed around the foci of contusion,the expressions of TNF-? and IL-1? in the model group were significantly higher than those in sham operated group,and the high expressions were sustained from 24 h to 72 h after brain injury.Compared with the model group,the levels of TNF-? and IL-1? in the brain of CIG treatment groups were obviously decreased in a dose-dependent manner and the inhibitory effects of TNF-? and IL-1? were more significant at 72 h after brain injury.CONCLUSION:CIG may have neuroprotective effect on traumatic brain injury through inhibiting the expression of inflammatory cytokines and reducing the inflammatory reaction.

Objective To investigate the effects of siRNA silencing of inhibitor of apoptosis protein gene on the proliferation of airway smooth muscle in bronchial asthma mice .Methods Tweenty female BALB/c mice were randomly divided into experimen‐tal group and control group .Mice in the experimental group were constructed asthma models .The trachea and bronchi were collect‐ed for cell culture .Respectively ,the cultured airway smooth muscle cells in the experimental group and the control group were grouped:PBS control group (added to PBS solution) ,missense chain control group (transfected with missense chain) and siRNA transfection group (transfected with siRNA Survivin) .The expressions of Survivin mRNA ,Survivin protein and caspase‐9 protein in airway smooth muscle cells were detected .The levels of IL‐6 and CCL5 in cell supernatant were detected .Results RT‐PCR re‐sults showed that ,the relative expression levels of Survivin RNA of airway smooth muscle cells in the siRNA transfection group of the experimental group were lower than the PBS control group and missense chain group ,the differences were statistically signifi‐cant (P<0 .05) .Western blot results showed that ,the expressions of Survivin proteins in the siRNA transfection group of the ex‐perimental group were lower than the PBS control group and missense chain control group ,while the caspase‐9 protein were higher , In the experimental group ,the synthesis and secretion levels of IL‐6 and CCL5 of airway smooth muscle cells in the siRNA transfec‐tion group were lower than the PBS control group and missense chain control group ,the cell proliferations at 2-4 d in the siRNA transfection group were lower than the PBS control group and missense chain control group ,the proportion of G0/G1 phase in the siRNA transfection group were higher than the PBS control group and missense chain control group ,but the proportion of S/G2 phase were lower than the PBS control group and missense chain control group ,cells apoptosis rates of the siRNA transfection group were lower than the PBS control group and missense chain control group ,the differences were statistically significant (P<0 .05) .Conclusion siRNA specific silencing Survivin gene could accelerate the airway smooth muscle cell apoptosis ,and inhibit cell abnormal proliferation and secretion .

OBJECTIVE:To discuss the problem in Chinese Traditional Medicine market and provide some countermeasures for its supervision. METHODS: We analyzed the status quo of Chinese traditional medicine market and the reasons accountable for the problem existing in Chinese traditional medicine market. RESULTS & CONCLUSION: The quality of Chinese traditional medicine and Chinese medicinal decoction pieces in Chinese traditional medicine market is far from perfect, it is urgent to tighten supervision on the quality of Chinese traditional medicine.

Medical science is the science about human.Humanistic attribute is one of the essence attributes of medicine.Since modern times,under the effect of the western biomedical modes,people have gradually neglected humanistic attribute,social responsibilities of medical workers and their humanistic care for patients,which results in a series of problems in current medical research and medical practice.The return of humanistic spirit is not only the essential requirement of the essence attribute of medicine,but also the calling from the development of modern medical science.Reconstruction of the humanistic spirit is the common responsibility for contemporary medical workers.Meanwhile,the cultivation of the medical students who have great humanistic quality should be the significant mission for the contemporary medical education.