Objective To study of vascular false as the clinical effect and safety of acromastitis were treated by com -pound anisodine treatment , to provide reference for the treatment of vascular pseudo optic .Methods From June 2013 to June 2015, 132 cases of patients with vascular pseudo optic papilla in our hospital were selected as the observation objects , and were divided into observation group and control group each 66 cases according to the treatment method .The control group was treated with routine drug therapy , and the observation group in the control group on the basis of daily injections of Com -pound Anisodine Hydrobromide Injection .Two groups before and after the treatment of vision changes , and the treatment of safety and efficacy were compared .Results The curative effect:The two groups of patients after treatment were improved , but the observation group was significantly higher than the control group after treatment ;The cure rate and total effective rate of the observation group were 39.51%and 88.89%respectively, which was significantly higher than the control group of 24. 36%and 73.08%, and had better curative effect in observation group .Security aspect:The total incidence of adverse reac-tions in the observation group and the treatment group was 10.61%, significantly less than 24.24% of the control group, higher safety .Conclusion In the treatment of vascular pseudoaneurysms as papillitis drugs , the curative effect of compound anisodine is much better with better security , which can significantly improve the visual acuity of the patients .

Epidermal growth factor receptor(EGFR) family plays an important role in the development of gastric cancer. EGFR-targeted therapeutic agents include extracellular monoclonal antibodies and intracellular tyrosine kinase inhibitors. Many new agents such as trastuzumab, cetuximab, panitumumab and lapatinib, have been tested in randomized phase Ⅲ clinical trials. Results from ToGA trial may shed some light on the treatment of advanced gastric cancer.

Objective:To study the effect of exogenous hyaluronidase on proliferation of human breast cancer cells in vitro.Methods:The proliferation of human breast cancer cell line ZR-75-30 were detected by MTT-assay and flow cytometry.Results:The absorbency value(A) of ZR-75-30 in study group treated with Hyase (0.674?0.221) was significantly higher than that in control group(0.563?0.046).The percentage of phase S cells in study group(18.36?0.65) was higher than that in control group(2.19?0.10);the percentage of phase G0/G1 cells in study group(44.49?4.33) was lower than that in control group(62.14?26.97).The absorbency value(A) of MDA-MB-435 in study group (0.674?0.221) was significantly higher than that in control group(0.563?0.046).The absorbency valve(A) of MDA-MB-231 in study group (0.674?0.221) was significantly higher than that in control group(0.563?0.046).Conclusion:Exogenous hyaluronidase may promote the proliferation of breast cancer cell lines in vitro.

Tumor progression is often associated with immune suppression or the ability of the tumors to avoid immune surveillance.Immunotherapy improves the ability of the immune system to recognize and clear tumor cells with a little influence on the normal tissues.Immunotherapy is a hot spot in the research of advanced esophageal cancer.Innnunotherapy of esophageal cancer includes immune checkpoint inhibitors,adoptive cellular immunotherapy,tumor vaccines and antibody therapy.At present,a large number of clinical trials are underway to evaluate the role of immunotherapy in esophageal cancer.Checkpoint inhibitors represented by Pembrolizumab and Nivolumab,has achieved initial success in the treatment of advanced esophageal cancer to improve the prognosis and life quality of esophageal cancer patients.In the future,further studies are needed to have a research on the effects of tumor heterogeneity,prediction of therapeutic targets,and immune tolerance.

Latrodectus tredecimguttatus (commonly known as black widow spiders) have toxins not only in their venom glands, but also in other parts of their body, in their eggs and even in the newborn spiderlings. The study on the toxins in venom and materials outside the venom glands of the spiders to elucidate their differences and similarities, evolutional relationship and biological functions is of important theoretical and applicable significance. The development of modern protein chemistry and proteomics techniques has provided efficient means for the study of protein and peptide toxins of L. tredecimguttatus. By using such techniques, the molecular base and action mechanism of the toxins can be revealed at the levels of both single purified proteins and omics. Up to now, although protein chemistry and proteomics study on L. tredecimguttatus toxins have achieved a certain progress, the relevant work particularly that on the toxins in the materials outside the venom glands has to be further deepened.
Animals ; Arthropod Proteins ; chemistry ; Black Widow Spider ; chemistry ; Proteomics ; Venoms ; chemistry

Objective To compare the therapeutic efficacy between conventional nursing and conventional nursing plus electroacupuncture in treating gastrointestinal dysfunction after abdominal operation, and their effects in recovering gastrointestinal function. Method Sixty patients with gastrointestinal dysfunction after abdominal operation were randomized into an electroacupuncture group and a control group according to their operation time, 30 cases in each group. The electroacupuncture group was intervened by conventional postoperative nursing plus electroacupuncture, by selecting bilateral Zusanli (ST36), Shangjuxu (ST37), Xiajuxu (ST39), Neiguan (PC6) and Taichong (LR3), connected to HANS electroacupuncture device, with frequency at 2 Hz and a tolerable intensity. The treatment was given once a day, for 5 d in total, till the recovery of gastrointestinal function. The control group was intervened by conventional postoperative nursing. The therapeutic efficacy was evaluated at the end of the intervention. The time of first bowel sound, first exhaust time, and first bowel movement time were observed. The gastrointestinal symptom score (GIS) was adopted to evaluate the effects of the two methods in improving gastrointestinal function at the end of the intervention. Result The general therapeutic efficacy of electroacupuncture group was better than that of the control group(P<0.05). In the electroacupuncture group, time taken for the first bowel sound, first exhaust and first bowel movement after the operation was shorter(P<0.05). The GIS dropped in both groups after the treatment, and the improvement was more significant in the electroacupuncture group(P<0.05). Conclusion Electroacupuncture helps promote the recovery of gastrointestinal function in patients with postoperative gastrointestinal dysfunction, and can produce a more significant improvement.

Objective To study the effect of ganoderma lucidum polysaccharides on T cell subsets and AQP1, AQP3 expression of bladder cancer T24 cell line bearing mice.Methods 60 BALB/C nude mice were selection as experimental animals, bladder tumor bearing animal models were made by bladder cancer T24 cells subcutaneous injection, and were randomly divided into control group, cisplatin group, ganoderma lucidum polysaccharides and cisplatin group, cisplatin group given 25 mg/kgcisplatin intraperitoneal injection, cisplatin combined ganoderma lucidum polysaccharide group given 200 mg/kg ganoderma lucidum polysaccharide lavage, 25 mg/kg cisplatin intraperitoneal injection, the control group given quite a volume normal saline lavage.Then tumor volume and weight, peripheral blood T cell subsets and AQP1, AQP3, Caspase-3, Bax mRNA content in tumor tissue were determined.Results Cisplatin group, cisplatin combined ganoderma lucidum polysaccharide tumor volume and mass were significantly lower than the control group (P<0.05), cisplatin and ganoderma lucidum polysaccharide tumor volume and mass were significantly lower than that cisplatin group ( P<0.05 ); Cisplatin group, cisplatin combined ganoderma lucidum polysaccharides group CD4 + T cells, CD8 +T cells , CD4 +/CD8 + were significantly higher than that of control group (P<0.05), cisplatin combined ganoderma lucidum polysaccharide group CD4 +T cells, CD4 +/CD8 +were significantly higher than that of cisplatin group (P<0.05); Cisplatin group, cisplatin combined ganoderma lucidum polysaccharide group mices tumor tissues Bax, Caspase 3 mRNA content were significantly lower than the control group (P<0.05), cisplatin combined ganoderma lucidum polysaccharide group Bax,caspase 3 mRNA content were significantly lower than that of cisplatin group (P<0.05); Cisplatin group, cisplatin combined ganoderma lucidum polysaccharide group mice tumor tissue AQP1, AQP3 mRNA content were significantly lower than the control group (P<0.05); Cisplatin combined ganoderma lucidum polysaccharide group AQP1, AQP3 mRNA content were significantly lower than cisplatin group (P<0.05 ). Conclusion ganoderma lucidum polysaccharide can inhibit tumor growth and enhance cellular immune function of bladder cancer T24 cell line bearing mice, and adjust the expression of pro-apoptotic genes, AQP1 and AQP3.

Objective:To evaluate the influence of sodium hypochlorite (NaOCl) solution used during root canal therapy on dentin bond strength.Methods:In the study,15 freshly extracted human third molars with complete dental crowns,caries and filling-free were selected.The occlusal enamel was removed perpendicular to the long axis of the tooth to expose middle flat surfaces of sound dentin.The occlusal dentin surfaces were then polished using 600-grit silicon papers for 1 min and rinsed with deionized water for 1 min.The teeth were randomly divided into three groups according to the treatment received:group A (negative control group),the samples were immersed in deionized water for 20 min;group B,the dentin surfaces were immersed in 2.50％ NaOCl solution for 20 min,with the solution being renewed every 5 min;group C,the dentin surfaces were immersed in 5.25％ NaOCl solution for 20 min,with the solution being renewed every 5 min.All the treated dentin surfaces were bonded using a self-etching adhesive system (SE bond) with a 5 mm in height resin composite (AP-X).After storage in deionized water at 37 ℃ for 24 h,the adhesive samples were sectioned longitudinally to produce 1.0 mm ± 1.0 mm stick specimens (n =45) for micro-tensile bond strength testing (MPa).Failure modes (adhesive failure,cohesive failure or mixed failure) at the dentin-resin interface were observed using a stereomicroscope.The micro-tensile bond strength data among the three groups were analyzed by a one-way ANOVA,then the Post-hoc test(LSD) was employed for pairwise comparison.The distribution of failure modes among the groups were analyzed by chi square test.Results:Significant decreased bond strength values were found for the 2.50％ NaOCl-treated group (26.04 ± 5.74) MPa and 5.25％ NaOCl-treated group (24.46 ± 3.77) MPa when compared with the strength of negative control group (48.71 ± 7.77) MPa,P =0.000.Compared with the negative control group,themicro-tensile bond strength of the 2.50％ NaOCl-treated group and 5.25％ NaOCl-treated grouphad dropped by 46.5％ and 50.2％.However,there was no significant difference of bond strength between the 2 NaOCl-treated groups (P =0.214).The distribution of failure modes showed significant difference in all the three groups (2 =56.324,P =0.000).The mixed failure (68.9％) was the most mode of fracture in the negative control group,followed by adhesive failure (24.4％),and the cohesive failure was leas t (6.7％).The proportion of adhesive failure mode was higher in NaOCl-treated groups than in negative control group (P =0.000).There was no significant difference of the distribution of failure modes between the 2.50％ NaOCl-treated group and 5.25％ NaOCl-treated group(P =0.197),and there was no cohesive failure mode detected in the two groups.Conclusion:The micro-tensile bond strength of dentin to composite resin was lower after exposure to NaOCl solution.

BACKGROUND:Human amniotic mesenchymal stem cel s have the potential to differentiate into insulin-secreting cel s and have immunomodulatory effects. Additional y, it has been clinical y proved that Gegen Qin Lian Tang can lower blood sugar. OBJECTIVE:To investigate the effects of Gegen Qin Lian Tang combined with human amniotic mesenchymal stem cel transplantation on rat early diabetic retinopathy, by detecting pathological changes, levels of blood indicators and expressions of vascular endothelial growth factor in retinal tissues. METHODS:By intraperitoneal injection of streptozotocin, 42 Wistar rat models with diabetes mel itus were prepared and then randomly divided into model group, human amniotic mesenchymal stem cel transplantation group (cel transplantation group) and combination of human amniotic mesenchymal stem cel s and Gegen Qin Lian Tang group (combination group). At 4 weeks after transplantation, levels of blood sugar and serum insulin in diabetic rats were detected;pathological changes of the retina in diabetic rats were observed by hematoxylin-eosin staining. Besides, expressions of CD45 and vascular endothelial growth factor mRNA were detected using immunohistochemistry staining and RT-PCR technology, respectively. RESULTS AND CONCLUSION:Compared with the model group, the levels of blood glucose and serum insulin in both cel transplantation and combination groups were significantly decreased and increased, respectively, and these changes were even more significant in the combination group (P<0.05). In the model group, the retinal edema could be found, accompanied by structural disorder and irregular cel arrangement, while these retinal lesions were relatively milder in the cel transplantation group and significantly improved in the combination group. In addition, the CD45 expression and in the retina was highest in the model group subsequently fol owed by the cel transplantation group (P<0.05) and lowest in the combination group (P<0.05). Furthermore, compared with the model group, the expression of vascular endothelial growth factor mRNA was also significantly lower in the other two groups, especial y in the combination group (P<0.05). These data show that Gegen Qin Lian Tang combined with human amniotic mesenchymal stem cel transplantation improves the severity of diabetic retinopathy by inhibiting expressions of CD45 and vascular endothelial growth factor in the retinal tissues.

OBJECTIVE: To optimize the macroporous absorbing resin which were of best action in adsorption and desorption on the active components in Naomaitong granules. METHODS: UV spectrophotometry and HPLC was employed to determine the adsorbability and desorption capacity of different macroporous absorbing resins on total anthraquinones, total ginsenosides, total alkaloids and Puerarin. RESULTS: There were differences in adsorption and desorption capacity on active components in Naomaitong granules among different macroporous absorbing resins. Considering the general adsorbability and desorption capacity of different macroporous absorbing resins, AB-8 turned out to be of the best purification effect on Naomaitong granules. CONCLUSION: The results serve as a theoretical basis for the production of Naomaitong granules.