Epidermal growth factor receptor(EGFR) family plays an important role in the development of gastric cancer. EGFR-targeted therapeutic agents include extracellular monoclonal antibodies and intracellular tyrosine kinase inhibitors. Many new agents such as trastuzumab, cetuximab, panitumumab and lapatinib, have been tested in randomized phase Ⅲ clinical trials. Results from ToGA trial may shed some light on the treatment of advanced gastric cancer.

Objective To study of vascular false as the clinical effect and safety of acromastitis were treated by com -pound anisodine treatment , to provide reference for the treatment of vascular pseudo optic .Methods From June 2013 to June 2015, 132 cases of patients with vascular pseudo optic papilla in our hospital were selected as the observation objects , and were divided into observation group and control group each 66 cases according to the treatment method .The control group was treated with routine drug therapy , and the observation group in the control group on the basis of daily injections of Com -pound Anisodine Hydrobromide Injection .Two groups before and after the treatment of vision changes , and the treatment of safety and efficacy were compared .Results The curative effect:The two groups of patients after treatment were improved , but the observation group was significantly higher than the control group after treatment ;The cure rate and total effective rate of the observation group were 39.51%and 88.89%respectively, which was significantly higher than the control group of 24. 36%and 73.08%, and had better curative effect in observation group .Security aspect:The total incidence of adverse reac-tions in the observation group and the treatment group was 10.61%, significantly less than 24.24% of the control group, higher safety .Conclusion In the treatment of vascular pseudoaneurysms as papillitis drugs , the curative effect of compound anisodine is much better with better security , which can significantly improve the visual acuity of the patients .

Tumor progression is often associated with immune suppression or the ability of the tumors to avoid immune surveillance.Immunotherapy improves the ability of the immune system to recognize and clear tumor cells with a little influence on the normal tissues.Immunotherapy is a hot spot in the research of advanced esophageal cancer.Innnunotherapy of esophageal cancer includes immune checkpoint inhibitors,adoptive cellular immunotherapy,tumor vaccines and antibody therapy.At present,a large number of clinical trials are underway to evaluate the role of immunotherapy in esophageal cancer.Checkpoint inhibitors represented by Pembrolizumab and Nivolumab,has achieved initial success in the treatment of advanced esophageal cancer to improve the prognosis and life quality of esophageal cancer patients.In the future,further studies are needed to have a research on the effects of tumor heterogeneity,prediction of therapeutic targets,and immune tolerance.

Objective:To study the effect of exogenous hyaluronidase on proliferation of human breast cancer cells in vitro.Methods:The proliferation of human breast cancer cell line ZR-75-30 were detected by MTT-assay and flow cytometry.Results:The absorbency value(A) of ZR-75-30 in study group treated with Hyase (0.674?0.221) was significantly higher than that in control group(0.563?0.046).The percentage of phase S cells in study group(18.36?0.65) was higher than that in control group(2.19?0.10);the percentage of phase G0/G1 cells in study group(44.49?4.33) was lower than that in control group(62.14?26.97).The absorbency value(A) of MDA-MB-435 in study group (0.674?0.221) was significantly higher than that in control group(0.563?0.046).The absorbency valve(A) of MDA-MB-231 in study group (0.674?0.221) was significantly higher than that in control group(0.563?0.046).Conclusion:Exogenous hyaluronidase may promote the proliferation of breast cancer cell lines in vitro.

Arginine ; therapeutic use ; Burns ; therapy ; Humans ; Nutrition Therapy ; Wound Healing

Burns ; Periodicals as Topic ; Surgery, Plastic

Objective To investigate the roles of Thl7 cells in the immunological pathogenesis of vitiligo. Methods Forty patients with progressive or stable vitiligo and 20 normal human controls were included in this study. Enzyme-linked immunosorbent assay (ELISA) was performed to measure the level of serum IL-17, and SYBR Green I real-time RT-PCR to detect the mRNA expression of RORγt in peripheral blood mononuclear cells (PBMCs) from these patients. The correlation of the above parameters with disease activity was assessed. Results The levels of serum IL-17 and ROR-yt mRNA in PBMCs were significantly higher in patients with progressive and stable vitiligo than in normal human controls (all P< 0.01). Conclusion Thl7 cells may be closely associated with the pathogenesis of vitiligo.

Objective:This study aims to investigate the expression pattern of miR-512-3p in breast cancer and noncancerous paired specimens as well as the effects of miR-512-3p on the proliferation, apoptosis, cell cycle, and cloning of MD-MBA-231 breast cancer cells. The study also aims to identify the miR-512-3p target gene. Methods:Reverse transcription-polymerase chain reaction (RT-PCR) was conducted to quantify the miR-512-3p expression in breast cancer and noncancerous paired specimens. Methylthiazol tetrazolium (MTT) assay, flow cytometry, and clone formation assay were used to characterize the function of miR-512-3p in breast cancer. Target prediction was performed using the TargetScan, PicTar, and miRanda software. The results were validated using RT-PCR and western blot target validation. Results:The relative expression of miR-512-3p in breast cancer specimens was significantly lower than those in normal breast specimens (P<0.05). MTT assay revealed that 48 h after transfection, miR-512-3p significantly repressed the proliferation of MD-MBA-231 cells at a suppression rate of 45.38%and at a concentration of 100 nmol/L. MiR-512-3p increased the percentage of early apoptotic cells in the treatment groups (9.32 ± 0.41)%compared with those in the blank controls (3.1 ± 0.54)%and in the negative controls (2.9 ± 0.39)%(P<0.05). Significant differences were found in the percentages of the G0/G1-and G2/M-phase cells after miR-512-3p transfection compared with those in the controls (P<0.05). In the cloning assay, clone formation was inhibited in the miR-512-3p-transfected groups compared with those in the control groups. RT-PCR and western blot results indicate that miR-512-3p significantly inhibited the c-FLIP mRNA and protein expression. Conclusion:MiR-512-3p expression is relatively decreased in breast cancer specimens compared with those in the normal samples. The negative effect of miR-512-3p on cell proliferation and clone formation and its positive effect on early apoptosis through c-FLIP targeting suggest that miR-512-3p acts as a tumor suppressor gene in breast cancer. Therefore, miR-512-3p may be a new target for the diagnosis and treatment of breast cancer in the future.

Objective:To evaluate the influence of sodium hypochlorite (NaOCl) solution used during root canal therapy on dentin bond strength.Methods:In the study,15 freshly extracted human third molars with complete dental crowns,caries and filling-free were selected.The occlusal enamel was removed perpendicular to the long axis of the tooth to expose middle flat surfaces of sound dentin.The occlusal dentin surfaces were then polished using 600-grit silicon papers for 1 min and rinsed with deionized water for 1 min.The teeth were randomly divided into three groups according to the treatment received:group A (negative control group),the samples were immersed in deionized water for 20 min;group B,the dentin surfaces were immersed in 2.50％ NaOCl solution for 20 min,with the solution being renewed every 5 min;group C,the dentin surfaces were immersed in 5.25％ NaOCl solution for 20 min,with the solution being renewed every 5 min.All the treated dentin surfaces were bonded using a self-etching adhesive system (SE bond) with a 5 mm in height resin composite (AP-X).After storage in deionized water at 37 ℃ for 24 h,the adhesive samples were sectioned longitudinally to produce 1.0 mm ± 1.0 mm stick specimens (n =45) for micro-tensile bond strength testing (MPa).Failure modes (adhesive failure,cohesive failure or mixed failure) at the dentin-resin interface were observed using a stereomicroscope.The micro-tensile bond strength data among the three groups were analyzed by a one-way ANOVA,then the Post-hoc test(LSD) was employed for pairwise comparison.The distribution of failure modes among the groups were analyzed by chi square test.Results:Significant decreased bond strength values were found for the 2.50％ NaOCl-treated group (26.04 ± 5.74) MPa and 5.25％ NaOCl-treated group (24.46 ± 3.77) MPa when compared with the strength of negative control group (48.71 ± 7.77) MPa,P =0.000.Compared with the negative control group,themicro-tensile bond strength of the 2.50％ NaOCl-treated group and 5.25％ NaOCl-treated grouphad dropped by 46.5％ and 50.2％.However,there was no significant difference of bond strength between the 2 NaOCl-treated groups (P =0.214).The distribution of failure modes showed significant difference in all the three groups (2 =56.324,P =0.000).The mixed failure (68.9％) was the most mode of fracture in the negative control group,followed by adhesive failure (24.4％),and the cohesive failure was leas t (6.7％).The proportion of adhesive failure mode was higher in NaOCl-treated groups than in negative control group (P =0.000).There was no significant difference of the distribution of failure modes between the 2.50％ NaOCl-treated group and 5.25％ NaOCl-treated group(P =0.197),and there was no cohesive failure mode detected in the two groups.Conclusion:The micro-tensile bond strength of dentin to composite resin was lower after exposure to NaOCl solution.

Objective To study the neuroelectrophysiological responsibility, mechanism and clinical application of auditory event-related potential (AERP) generated in bilateral auditory centers with ipsilateral and contralateral recordings. Methods 14 normal young adults were served as subjects. AERPs, including the responsibility, latencies, amplitudes and waveforms, were simultaneously recorded on ipsilateral and contralateral vertexes and analyzed. Results The latencies and amplitudes of AERPs did not show significicant difference statislically between ipsilateral and contralateral vertexes recordings. However, the responsibility of AERP recorded on ipsilateral vertex was more satisfied than that on contralateral vertex, which exhibited smoothing waves, discriminative wave peaks and little hetero-wave. Conclusion The origins of AERP in bilateral auditory centers were essentially symmetrical. Nevertheless, the responsibility of AERP recorded on ipsilateral vertex was more satisfied than that on contralateral vertex, which was related to the components of auditory subcortex, transmission of auditory nerve to bilateral centers and the contralateral inhibiting effect of medial olivo-cochlear system. The effects of multi-generation and multi-component of AERPs on the clinical application should be considered.