Child ; Child, Preschool ; China ; Drug Therapy ; standards ; Drug-Related Side Effects and Adverse Reactions ; Humans ; Infant ; Pediatrics ; Pharmacology, Clinical ; Pharmacovigilance ; Societies, Pharmaceutical

Decorin( DCN) is one of the leucine-rich proteoglycan family of small molecules.It is recog-nized as a tumor suppressor gene.DCN can inhibit tumor cell proliferation and metastasis by binding and inactiva-tion of transforming growth factor beta( TGF-β) and inhibiting the expression of vascular endothelial growth fac-tor or by activating the signaling pathway that promotes cell proliferation by EGFR/MAPK/p21.it plays an impor-tant effect on tumor development,angiogenesis and metastasis.DCN is expressed in various malignant tumors,By up or down regulation,DCN can exert its anti tumor activity,as well as reducing or delaying the occurrence and development of many kinds of malignant tumor.DCN gene may be a potential target for the treatment of many kinds of malignant tumors.

OBJECTIVE:To investigate the characteristics and rules of leukocytopenia induced by essential antipsychotic medi-cines in psychotic in our hospital,and provide reference for the clinical treatment. METHODS:1 345 psychotics received essential antipsychotic medicines from Nov. 2012 to Nov. 2014 in our hospital were collected,and retrospective analysis was adopted to sta-tistically analyze the age,sex and the drug type,dose and treatment time that caused leukocytopenia,symptom duration,white blood cell(WBC)count,clinical manifestation and prognosis. RESULTS:In the 1 345 patients,there were 205 patients with WBC sustained reduction in the process of medication,accounting for 15.24%. The incidence rate of leukocytopenia in patients older than 60 years old reached up to 54.15%;57 patients with leukocytopenia was induced by combination medication,accounting for 20.14% in the total cases of combination medication,and it was significantly higher than the incidence rate of single medication (13.93%),the difference was statistically significant(P<0.05);incidence rate of leukocytopenia induced by the first generation of antipsychotic medicines was 63.36%,which was significantly higher than the second generation(36.64%),the difference was sta-tistically significant(P<0.05);there were 113 patients with the WBC count of 3.0×109-3.9×109 L-1,accounting for 54.63%,com-pared with other WBC count groups,there were significant differences(P<0.05);most of the patients had no discomfort and obvi-ous clinical symptoms,and all patients were improved or recovered after symptomatic treatment. CONCLUSIONS:The incidence rate of antipsychotic in elderly patients with essential antipsychotic medicines is relatively high,and the incidence of antipsychotic is more likely to be caused by combination medication and the first generation antipsychotic medicines. Although leukocytopenia can be cured,it may increase the risk of infection,so blood routine should be regularly checked and paid great attention during treatment.

Objective To investigate the clinical significance of detection of serum IL -6,IL -17, GM-CSF and TNF -αlevels in patients with rheumatoid arthritis.Methods A retrospective research was made in 79 cases with rheumatoid arthritis,and they were divided into stable period and active period,and another 40 healthy persons were chosen as control group.IL -6,IL -17,GM-CSF and TNF -αlevels were compared,and the relevance was investigated.Results CRP,ESR and DAS28 of active period [39.14 (35.81 )mg/L,41.92 (33.27)mg/L, 4.85(2.74)]were higher than those of the stable period[21.07(16.53)mg/L,29.57(25.14)mg/L,2.88(1.76)] and control group[5.92(2.80)mg/L,3.19(2.24)mg/L],the differences were statistically significant (F =7.013, 6.224,2.136,all P <0.05).Serum levels of GM-CSF,IL -6 and TNF -αof active period[33.81(26.97)pg/mL, 47.23(42.81 )pg/mL,75.35 (41.69 )pg/mL]were higher than those of control group [6.19 (5.11 )pg/mL, 4.68(5.21)pg/mL,33.18 (20.94)pg/mL]and stable period [12.81 (9.76 )pg/mL,8.46 (10.37 )pg/mL, 43.05(38.14)pg/mL],the differences were statistically significant (F =5.328,6.117,8.016,all P <0.05).And there was no significant difference in IL -17 level between the groups (F =0.776,P >0.05).No correlation was observed between IL17 and DAS28,GM-CSF and IL -6 (r =0.213,0.167,0.316,all P >0.05),TNF -αand DAS28,GM-CSF,IL -6 and IL -17 (r =0.761,0.729,0.549,0.421,all P <0.05 ),IL -6 and DAS28 and GM-CSF (r =0.648,0.626,all P <0.05),GM-CSF and DAS28 had good correlation (r =0.563,P <0.05). Conclusion IL -6,GM-CSF and TNF -αlevels were positively correlated with the progression of rheumatoid arthritis,joint detection is of great value in monitoring the disease.

Objective To realize current status of healthcare-associated infection (HAI)in a neonatal intensive care unit(NICU)of a hospital,and evaluate the effect of comprehensive intervention measures.Methods HAI in NICU was monitored,targeted intervention measures were implemented,incidence of HAI before and after inter-vention was compared.Results The implementation rates of intervention measures at intervention stage was higher than that of pre-intervention stage,the difference was significant(all P <0.001 ).Incidence of HAI at intervention stage was lower than that of pre-intervention stage(5.64%[46/816]vs 13.45% [46/342],χ2 =20.12,P <0.001). The main infection site was lower respiratory tract (38.04% [35/92]).Incidence of ventilator-associated pneumonia and catheter-related bloodstream infection after intervention were both lower than pre-intervention stage (0.38‰ vs 3.30‰,0.12‰ vs 1 .18‰,respectively).The main isolated pathogens before and after intervention were both gram-positive bacteria,accounting for 58.09% and 51 .16% respectively.Conclusion Routine HAI monitoring can reflect the weak links in infection control practice,HAI can be controlled effectively by carrying out intervention measures.

OBJECTIVE:To establish an HPLC method for determination of Emodin in Yanyan granules.METHODS:The stationary phase was Diamonsil C18(150mm? 4.6mm,5? m)column and the mobile phase was methanol-0.1% phosphoric acid(68∶ 32),with detection wavelength set at 254nm.RESULTS:Within the range of 0.26～ 3.90? g Emodin had a good linearity,and the average recovery was 96.73%(RSD=1.49%).CONCLUSION:The method is accurate,sensitive,reproducible,simple and can be used for the quality control of Yanyan granules.

Education of the adolescent includes sexual physiological education,sexual psychological education and sexual moral education.of both the sexual psychological education and sexual moral deucation,which are often ignored,are the very important two aspects.

AKI is one of the frequent complications of critically ill patients,which is associated with high mortality.RRT is the main treatment,especially CRRT nowadays.But the optimal dosage of CRRT is unclear.In this article,we will review the evidence of CRRT in critically ill patients with AKI.Compared with traditional dosage of CRRT,high dose CRRT did not improve the clinical outcome.There was no difference in mortality rate,time for renal recovery,ICU hospitalization time,or organ failure in both groups,while there were more complications including hypokalemia and hypophosphatemia in the high dose CRRT group.Treatment with high dose CRRT could alter drug metabolism,cause malnutrition,unbalance the electrolyzes and cause hypothermia.So we conclude that traditional dosage of CRRT[20～35 mL/(kg?h)]is applicable for critically ill patients with AKI,but the determination of optimal dose depends on many clinical situations other than clearance of small solutes.

[Objective] To observe the effect of electroacupuncture (EA) on learning and cerebral heme oxygenase (HO) activity in rats with vascular dementia (VD). [Methods] Sixty rats were randomly allocated to mimic group (Group A), EA group (Group B), nimotone group (Group C) and model group (Group D) . Rat models of VD were established by 4 - vessel occulusion (4 - VO) method. Ten days after modeling, Group B was trested with EA on Baihui (GV20) and Dazhui (GV14), one time per day; Group C with nimotone, 20ml/kg body weight, ig, qd. The treatment course lasted 15 days. And then the learning was estimated with Morris water maze and HO activity in cortex and hippocampus (HC) of rats was also measured. [Results] Mean escape latency within 6 days in EA group was shorter than that in model group ( P 0.05). In EA group, the swimming times in the original platform quadrant were more than that in the right, left or opposite quadrant and also more than that in the original platform quadrant in the model group (P

Objective:To research the effect of total saponins Panax ginseng (TSPG) on the drug resistance of human erythroleukemia drug resistant cell line K562/A02 and its mechanism.Methods:The sensitivity of TSPG-treated K562/A02 cells to anticancer drugs was determined by MTT assay.Immunohistochemistry method was used to measure mdr-1 gene product P-gp and apoptosis regulation gene product bcl-2 expression. RT-PCR technique was used to examine mdr-1 mRNA expression.Results:In non-toxic dosages, TSPG strengthened the toxicity effect of doxorubicin on K562/A02 cells, while the reversing effect of TSPG was related to its concentration. Decrease in P-gp expression in K562/A02 cells was marked ( P O. 05)after 3 days of TSPG(150?g/ml)treatment.Decrease in mdr-1 mRNA expression was significant ( P