Objective:To explore the safety profile of telmisartan based on the data from the literatures on its adverse drug reactions(ADRs).Methods:The literatures about clinical data of ADRs of telmisartan from 2004 to 2008 in China, collected by the databases of China Hospital Knowledge Database,Wanfang Data and VIP Information,were reviewed. Results:Of 1197 patients receiving telmisartan,95(7.94%) had ADRs involving six systems such as nervous system, cardiovascular system,gastrointestinal system,musculoskeletal system,and so on.The ADR incidences of oral 40 mg?d~(-1) administration and 80 mg?d~(-1) administration were respectively 5.29%(31/586) and 8.96%(42/469) and only 1 case (0.08%) was forced to stop his or her medication due to serious ADRs.Conclusion:Telmisartan was generally safe,and was generally well tolerated for its low incidence of adverse reactions,and therefore,there was no need to interrupt the treatment.The recommendation dose was locked in 40 mg?d~(-1).In clinical application of telmisartan,special attention should be paid to the ADRs of the nervous system and cardiovascular system.It is necessary to closely observe the serumal potassium level and orthostatic hypotension.

Objective To observe the effects of angiotensin Ⅱ(AngⅡ) AT 1 receptor antagonist,valsartan and mineralocorticoid receptor antagonist,spironolactone on growth factors and type Ⅰ collagen in aorta in spontaneously hypertensive rat(SHR).Methods Six-week male SHRs were divided into three groups at random:SHR control group,valsartan group and spironolactone group;six homogenous Wistar Kyoto(WKY) rats was served as normal control group.Valsartan 30mg?kg -1 ?d -1 ,spironolactone 20mg?kg -1 ?d -1 were respectively administered to rats in valsartan group and spironolactone group.Expressions of transforming growth factor ? 1(TGF ? 1),hepatocyte growth factor(HGF) and type Ⅰ collagen gene mRNA in aorta were determined by RT-PCR.Results Compared with those of untreated SHR group,levels of TGF?1 and type Ⅰ collagen gene mRNA expression in aortas treated with valsartan and spironolactone for 13 weeks were significantly reduced(P

Objective To evaluate performance of the clinical laboratories for detection of TORCH immunoassay. Methods There were 2 times external quality assessment (EQA) for nuclear antibody detection. Each panel consisting of 5 liquid serum samples were distributed. Each participants of the EQA program had to reply the results, the methodological procedure and the kits. All data were analyzed and then provided to all laboratory in our EQA program. Results In 2007, the rate of good response were more than 80% for HSV immunoglobulin-M, Rubella immunoglobulin-M and CMV immunoglobulin-M. Response for Toxo immunoglobulin-M was poor (53.1%). The sensitivities of the commercial kits were quite low (<80%). Some clinical laboratories using the same kits gave quite different S/CO value. Conclusions A lot of clinical laboratories get good score in TORCH detection external quality program. False negative is the major problem.

Objective To investigate the influence of nucleic acid extraction by boiling the serum samples and purifing nucleic acid from serum on HBV DNA detection by PCR. Methods The DNA templates from 2 serum samples with different HBV DNA concentration and 3 serum samples with hemolysis and non hemolysis for PCR were prepared by two kinds of method, i.e. by boiling the serum to release HBV DNA from the virus particles and by purifing the nucleic acid from serum. Then,the difference of reproducibility and HBV DNA quantitative values by PCR using the templates prepared by the two nucleic acid extraction methods was compared.Results HBV DNA quantitative values by PCR using the templates purified from the two samples were more reproducible( CV : 0.2165 and 0.3262, respectively) than those using the templates prepared by boiling the serum ( CV : 0.0699 and 0.1092, respectively). Moreover, when the concentration of hemoglobin in serum samples was higher than 5.89 ?g/L, the HBV DNA quantitative values by PCR using the templates purified from the samples were about one hundred fold more than those using the templates prepared by boiling the serum ( P

Objective To compare the real time flourescent quantitative HCV RNA PCR detective method with automated quantitative PCR (COBAS Amplicor) test. Methods The test panel consisted of 36 samples including one half ten fold dilution series of 7 samples, 20 HCV RNA positive samples, and 9 negative samples. We compared the two quantitive method, with their agreement for test results, intra assay variability, and linearity. Results The regression coefficient of linearity of COBAS Amplicor and real time flourescent quantitative method was 0.999 5 and 0.973 2, respectively. The inter assay coefficient of variation of COBAS Amplicor was lower than that of the real time flourescent quantitative method. A correlation co efficient between the HCV RNA titres as detected by the two methods was 0.845 ( P

Objective To study the clinical application of laparoscopic operation and the morbidity and their prevention. Methods 21069 cases of laparoscopic surgery were reviewed retrospectively. Results Laparoscopic operation was used in 19 conditions including cholecystectomy (21002),hepatic operation (21), common bile duct calculi (3), spleen trauma (1),kidney cyst (3),gynoplasty (16),chest surgery (2), intestinal adhesion (14),assistant Miles (3),appendectomy (2),mesentery teratoma (1).Major complications (60) (0.29%) included injury of choleduct (18), bile leakage (18),hemorrhage (17),the injury of gastrointestinal tract (7). Conclusions Laparoscopic operation may be in a more extensive seope. The major complications can be reduced by strict procedures of laparoscopic operation.

Internal mammary lymph node irradiation (IMLNI) could reduce local recurrence and distant recurrence and improve survival.The NCCN Guidelines have updated the recommends in IMLNI.However, the relative toxicities of IMLNI to the heart and lungs should be carefully considered by clinicians, so individualized indications for IMLNI are needed.Internal mammary sentinel lymph node biopsy (IM-SLNB) could be an accurate technique to guide IMLNI with minimally invasive staging, and provide more survival benefits to patients.This article reviews the benefits of IMLNI, controls of the side effect, and discussion of IMLNI guided by IM-SLNB.

Objective To investigate the relationship of the intensity of Schistosoma japonicum infection with the variation in individual egg counts. Methods Stool specimens were collected from residents in a village in Jiangxi Province and examined with Kato-Katz thick smear method for seven consecutive days. Two smears were prepared and microscopically examined for each specimen. 570 individuals completed the investigation. Coefficient of variation (CV) was used to assess the intra-individual variation of the egg counts, in relation to the demographic characteristics including age,sex,occupation,and the intensity of infection. Results The proportion of individuals with at least one positive finding increased from 33.0% in a single measurement to 56.5% by seven measurements (P0.05). Conclusion The prevalence and intensity of S. japonicum infection by single Kato-Katz test was obviously lower than the real figures. The intensity of infection may be an essential factor that affects the intra-individual variation of egg counts.

Objective To evaluate the performance of antinuclear antibody (ANA) detection in clinical laboratories. Methods There were 2 external quality assessments ( EQA) for nuclear antibody detection. The panel consisting of 5 liquid serum samples was distributed. Each participants of the EQA program should include in their report the results, the methodological procedure and the kits used . All data were analyzed then distributed to all laboratories in our EQA program. Results During 2003 to 2005, more than 70% laboratories detected antinuclear antibody by using indirect immunofluorescence(IIF) method and the rate of acceptable response list were 94. 1% , 93. 0% and 98. 6% , respectively. We have got better result from signals fluorescence pattern such as homogeneous and speckled than multiplicity samples. A wide dispersion of ANA titers was obtained every year. We neceived different rate from different methods of anti-ENA detection and 90% laboratories used immunoblot method. For anti-dsDNA most participants gave us acceptable performance. Conclusions ANA detection in routine practice is far from being standardized. However, EQA can have an impact on ANA detection performance if it provides advice for participating laboratories to limited laboratory variations related to method, procedure and kits.

Objective: To analyse the composition and content of the fatty acid and unsaponifiable matters in both tartary buckwheat seed oil and buckwheat seed oil. Methods: Fatty acids were obtained by the methods of KOH-EtOH(95%) saponification and treatment of inorganic acid. They were analyzed and determined by gas chromatography and GC/MS chromatography respectively. Results: 4-5 kinds of fatty acid were determined in both oils, and the content of unsaturated fatty acids in tatary buckwheat oil was 83.2% ( oleic acid 47.1%, linoleic acid 36.1%), buckwheat oil was 81.8% ( oleic acid 35.8% , linoleic acid 40.2% and linolenic acid 5.8%). The content of unsaponifiable matters in tartary buckwheat seed oil was 6.56%(?-sitosterol 54.37%), and in buckwheat seed oil was 21.90%(?-sitosterol 57.29%,?-tocopherol 1.41%). Conclusion: Both buckwheat oils were functional vegetable oils.