With an animal model of acute experimental myocardiac ischemia we observed the change of its myocardiac ultrastructure under the electron microscope. It has proved that clonidin has effects in relieving and postponing the pathogenis courses.

The normal and ischemic dog myocardia were treated with freeze-etching method and observed by transmission electron microscope, with special reference to the ultrastructural changes of myocardial cell membrane.Following 6 hours of coronary occlusion, the myocardial samples were taken from central and borderline ischemic areas as well as from distant normal area. The samples were cut into slices of about 1.5?1.5?3 mm~3 in size and proceeded by the routine freeze etching procedure.The myocardial cell membrane were fractured into the P face and E face. In normal myocardial cell membrane, the number of intramembranous particles of the P face was exceedingly larger than that of the E face. There were numerous caveolae appearing as depressions on the P face and elevated structures on the E face. In central and borderline ischemic areas, intramembranous particles were significantly decreased and aggregated. Following coronary occlusion, the caveolae of the myocardial cell membrane were found to be decreased, deformed or absent. In the severe ischemic area, spherical elevations with "rosette" configuration were occasionally observed as elevation on P face and depressions on E face.

The normal rabbit pancreas was treated with DMSO freeze-cracking method and observed by scanning electron microscope, with special reference to examine the ultrastructure of pancreatic exocrine portion.The pancreatic acinus was composed of five to six pyramidal acinar cells around the lumen. A few short microvilli were present at the apex of each acinar cell. The spherical nucleus was located in the basal region, with one to three nucleoli.Numerous rough surfaced endoplasmic reticula were found in the basal region of acinar cells arranged either in longitudinal arrays, or concentrically. Mitochondria were located between rough surfaced endoplasmic reticula. The rough surfaced endoplasmic reticulum may extend into supranuclear portion where well-developed Golgi apparatus and numerous spherical zymogen granules were situated.

18F-fluorodeoxyglucose (FDG) is the most commonly used broad-spectrum tumor imaging agent, but there are certain limitations in the application of some tumors. Fibroblast activation protein (FAP) is selectively expressed in more than 90% of malignant epithelial tumors, while it is hardly expressed in normal tissues and benign tumors. FAP inhibitors (FAPIs) specifically bind to FAP and selectively target FAP-positive tissues. In recent years, it has been reported that radionuclide-labeled FAPIs, such as 68Ga-FAPIs, are safe and reliable in tumor imaging and have low tissue background. They can also be used to image a variety of solid tumors, which are expected to be new tumor broad-spectrum imaging agents and play a greater role in clinical applications. This article reviews the recent progress of FAPIs in tumor imaging and diagnosis at home and abroad.

Objective To observe the effects of Sanhuangtangshenkang particles on the expression levels of tumor ne?crosis factor (TNF)-α, interleukin (IL)-6 in bone tissue of type 2 diabetic (T2DM) rat model. Methods There were seven groups of rats in this study. Ten female Wistar rats were used as control. Ten rats were established for osteoporosis model, and fifty rats were established for diabetic model. Fifty diabetic model rats were further divided into model control group, tra?ditional Chinese medicine (TCM) prevention group (high-dose TCM group, low-dose TCM group) and Western medicine group, with 10 rats for each group. All rats were given the corresponding intervention for 20 weeks. Automatic biochemistry analyzer was used to determine fasting blood glucose (FBG). ELISA was used to detect serum TNF-αand IL-6. Dual-energy X-ray absorptiometry (DXA) was used to assess bone density. Real-time PCR was used to appraise the mRNA of TNF-αand IL-6 in bone tissue. Western blot assay was used to measure protein expressions of TNF-αand IL-6 in bone tissue. Re?sults Compared with osteoporosis model group, bone density was significantly increased, serum levels of TNF-αand IL-6, the mRNA and protein expression level of IL-6 were significantly decreased in bone tissue of TCM groups and Western medi?cine group (P<0.05). The expression levels of TNF-αmRNA and protein were significantly decreased in bone tissue of in high-dose TCM prevention group and Western medicine group (P<0.05). Compared with the diabetic model group, the se?rum levels of FBG, TNF-αand IL-6 were significantly decreased in TCM groups and Western medicine group (P<0.05). The expression levels of TNF-αmRNA and protein were significantly decreased in high-dose TCM prevention group and Western medicine group (P<0.05). The mRNA and protein expression levels of IL-6 were significantly decreased in low-dose TCM prevention group and Western medicine group (P<0.05). Conclusion The expression levels of TNF-αand IL-6 are increased in serum and bone tissue of diabetic rats. Sanhuangtangshenkang particles can improve the inflammatorystate to play prevention and therapeutic effect on diabetic rats.

Acoustic Stimulation ; Evoked Potentials, Auditory, Brain Stem ; Humans ; Speech

Objective To evaluate the effects of Lipo prostaglandin E1(Lipo-PGE1) on the lower limb blood vessel in diabetic patients.Methods The study was performed in 36 patients with diabetes related lower limb vascular disease.Before and after Lipo-PGE1 treatment for 28 days,vascular diameter and blood flow velocity of femoral artery,popliteal artery,posterior tibial artery and dorsal pedal artery were measured by Doppler ultrasonography examination.Results After the treatment with Lipo-PGE1,the vascular diameters of femoral artery,popliteal artery,posterior tibial artery and dorsal pedal artery were dilated;and there were significant differences in posterior tibial artery and dorsal pedal artery.After the treatment with Lipo-PGE1,blood flow velocity of femoral artery,popliteal artery,posterior tibial artery and dorsal pedal artery were increased,however,there were no significantly statistical differences.After the treatment with Lipo-PGE1,all the patients feel well.Painless walking distance and maximal walking distance of 18 patients with intermittent claudication were increased.Conclusion Lipo-PGE1 causes a significant improvement of diabetes related lower limb vascular disease.Furthermore,the results demonstrated the major effects on posterior tibial artery and dorsal pedal artery.Lipo-PGE1 can dilate the smaller artery,which is important and helpful for patients to improve symptoms and signs.

Objective To understand hypertension risk factors among adult residents in Hebei province.Methods The adult residents were selected by multistage stratified sampling,and investigated with the same questionnaires,body measurement,blood pressure measurement,blood sample analysis,to analyze risk factors of hypertension by ?2 with simple factor and by logistic regression with mult-factors.Results The prevalence of hypertension in 4200 adult residents(18-69 ages) was 39.6% and age-adjusted rate was 28.5%.The single factors analysis indicated that the prevalence of hypertension was higher in men than in women,in countryside than in city,and increased with age rising.Its prevalence is correlated with education level,marriage status,occupation,income,smoking,passive smoking,manual activity,eating habits,BMI,waist,diabetes,blood fat,status of spirit and health.Multiple logistic regression analysis indicated that city,female,educational level of senior high school and above that,farm working,eating less brined vegetables is protective factors for hypertension,and their OR(95%CI) were 0.777(0.644~0.937),0.674(0.551~0.823),0.654(0.534~0.801),0.826(0.684~0.997),0.844(0.729~0.977) respectively,and more than 35 ages,not usually eating vegetable,bad status of health by self-sensations,high serum TC and TG,overweight and obesity were the main risk factors for hypertension,and their OR(95%CI) were 4.145(3.263~5.266),4.460(1.049~18.969),1.234(1.068~1.425),1.400(1.200~1.634),1.420(1.074~1.878),1.478(1.235~1.768),1.728(1.448~2.061) respectively.Conclusion The prevalence of hypertension in adult residents in Hebei province was correlated with multi-factors,so complicated prevention and control in hypertension should be reinforced.

Objective: To investigate the level and influencing factors of mental health literacy among middle school students in Taizhou City, Zhejiang Province, so as to provide the basis for the development of mental health education and intervention. Methods: Middle school students were selected from six counties (districts) in Taizhou City using the random cluster sampling method. Demographic information, disease history, learning status, and relationship with parents were collected through questionnaire surveys. The level of mental health literacy was investigated using the Structured Mental Health Literacy Questionnaire for Middle School Students. The influencing factors for mental health literacy was analyzed using a multiple linear regression model. Results: A total of 7 173 middle school students were surveyed, including 4 273 junior high school students (59.57%) and 2 900 high school students (40.43%), and had a mean age of (15.52±1.49) years. There were 3 644 male students (50.80%) and 3 529 female students (49.20%). The median score of mental health literacy was 83.00 (interquartile range, 23.00) points. Multiple linear regression analysis revealed that older age (β'=0.074), female gender (β'=0.038), living in urban areas (β'=0.029), good family economic status (β'=0.061), engaging in sports (β'=0.122), having a good relationship with the father (β'=0.128), and having a good relationship with the mother (β'=0.094) were associated with higher mental health literacy scores among middle school students; while having a history of disease (β'=-0.144) and learning difficulties (β'=-0.178) were associated with lower mental health literacy scores. Conclusion The level of mental health literacy among middle school students in Taizhou City is relatively good, and is influenced by demographic characteristics, disease history, sports participation, learning and relationships with parents.