BACKGROUND: Zirconia/alumina ceramic composite with excellent corrosion resistance, high strength and toughness has wide application prospect.OBJECTIVE: To investigate the effect of zirconia/alumina ceramic composite on proliferation, apoptosis and bone induction activity of human periodontal ligament cells.METHODS: Human periodontal ligament cells at passages 3-5 were cultured routinely as control group and co-cultured with zirconia/alumina ceramic composite as experimental group. After 1-5 days of culture, MTT,TUNEL, immunohistochemical detection, and alkaline phosphatase staining were used to detect cell proliferation,apoptosis, type I collagen expression and osteogenic activity, respectively.RESULTS AND CONCLUSION: Within 1-5 days of culture, there were no differences in cell proliferation, apoptosis and alkaline phosphatase activity between the two groups. At 5 days of culture, the type I collagen expression showed no difference between the two groups. Taken together, the zirconia/alumina ceramic composite has no effect on the proliferation, apoptosis and bone induction activity of human periodontal ligament cells.

Objective Twenty- four case of non- traumatic paralysis of radial nerve were reviewed. Methods Between 1985 and 2000, 24 patients with non- traumatic paralysis of radial nerve were referred to this hospital for treatment. There were 18 men and 6 women with a mean age of 38 years (range, 10 to 65 years). Eleven patients had occupations with unduly stress of the forearm. Tendon transfer was performed in 1 patient, operative neurolysis in 18, nerve- grafting in 3, neurorrhaphy in 2. Results Of the 12 patients with the palsy attributed to entrapment of the fibrous edge of muscles, 8 occurred at the supinator, 1 at the tendinous edge of extensor digitorum communis and 3 at multiple sites.The palsy was caused by marked constrictions of the nerves in 5 patients, by benign tumors or tumorous conditions in 4 (by a ganglion in 2, by lipoma in 1 and by haemangioma in 1), by a neurilemmoma on the nerve in 1. The cause was unknown in 1. The patients who accepted neurolysis were followed up from 6 months to 15 years postoperatively. An excellent or good functional result was documented in 14 patients, fair in 3, and poor in 1. The patients who were treated with neurorrhaphy or nerve- grafting were followed- up from 4 months to 6 years, 3 recovered completely, 2 were in the convalescent stage (1 was treated by nerve- grafting and the other by neurorrhaphy). Conclusion The etiology of non- traumatic paralysis of radial nerve was miscellaneous. The results of surgical treatment were satisfactory.

Objective To study the diagnosis and treatment of the inappropriate antidiuretic hormone secretion(SIADH) syndrome after cerebral injury. Methods A retrospective analysis was conducted on 12 patients suffered from SIADH after cerebral injury. The clinical features were similar to common hyponatremia, no specific manifestation. Most of the hyponatremia were detected by routine examination. The first of all,sodium losing in these patients with hyponatremia was routine supplied according the amont of true salt losing.If natremia was not raised or still more descended 2～3 days after treatment, and amount of supplying salt was correspond to that of natriuresis, SIADH should be considered,using restricting water therapy,substituted for salt supplement.furosemide plus albumin were the first choice for dehydration therapy. Results 24～48h after restricting water and natrium, 12 patient's natremia level was back up in different degree. Except for 2 death whose natremia was not corrected completely, 8 patient's natremia was corrected completely in 1 week, 1 patient's in 14 days, and 1 in 3 months after injury. Conclusions Diagnosis of SIADH is very difficult before treatment, but effective treatment can be obtained if we adopt correcting strategy. In these patients, the diagnosis of SIADH was confirmed with the course of treatment,we call it as therapeutic diagnosis.

Objective:To explore the relationship between friendship quality and loneliness and its influent factors like the measuring tool of friendship quality,the participants groups and cultural background. Methods:The studies on the association between the friendship quality and loneliness were collected through the databases of Chi-na National Knowledge Infrastructure,Wan Fang,Wiley Online library,Web of Science,Google Scholar and Duxiu from 1984 to 2014. Meta-analysis was conducted with the use of the CMA2. 0. Estimated value (r)and their 95%confidence interval (95%CI)was calculated to analyze the association between the friendship quality and loneliness from normal subjects except physiological disease (such as autism,etc. ). Results:Totally 46 literatures were met the requirements of meta-analysis with sample size of 19422,including 24 researches With 11846 participants who were from eastern countries,and 22 studies with 7676 western people. There was significant negative correlation of friendship quality and loneliness (r=-0. 28,95%CI:-0. 23--0. 32). The friendship quality measuring tool was able to adjust the relations of friendship quality and loneliness (Q=11. 95,P<0. 01). The participants groups had a regulatory role on the relationship between the friendship quality and loneliness (Q=19. 54,P<0. 001). Under dif-ferent cultural background of the subjects,the relationship between their friendship quality and loneliness was notsignificant difference (Q=0. 05 ,P>0. 05 ). Conclusion:It suggests that friendship quality has an influence on lone-liness. Measuring tools and subjects groups effect the relationship friendship quality and loneliness except cultural background.

Objective To evaluate the safety and effectiveness of circumferential pulmonary vein(PV) isolation in cases with persistent/permanent atrial fibrillation(AF) guided by the EnSite-NavX system.Methods Sixty cases(43 males,mean age 59.2?13.6 years) with persistent/permanent AF were enrolled from September 2004 to August 2005.The mean duration of AF was 4?6 years(6 months-24 years).The mean left atrium diameter was 43.2?6.4(42-58)mm.Circumferential PV isolation was performed guided by the EnSite-NavX system,combined with linear ablation of the superior-posterior wall of left atrium and mitral isthmus.Results All 60 cases underwent the procedure successfully,with the mean procedural time 170?34(150-240)min and the mean fluoroscopic time 23?10(12-45)min.The pulmonary vein isolation rate for the left ipsilateral PVs was 83.3% and was 78.3% for the right PVs.Segmental ostial ablation was applied for the rest cases.AF was terminated in 7 cases and was converted to atrial tachycardia(AT) or atrial flutter(AFL) in 5 cases.Sinus rhythm was restored by cardioversion in 48 cases.Post-ablation AT/AFL was documented in 10 cases (16.7%) which diminished automatically in 8 cases,and was abolished by re-ablation in 2 cases.After a mean of 6.5?3.2(4-11)months of follow-up,43 cases (71.7%) were free of AF(18 cases with anti-arrhythmic drugs).Complications: pseudo-aneurysm of femoral artery was found in 1 case and was cured after proper treatment.Conclusion Circumferential PV isolation and combination with left atrial linear ablation guided by the EnSite-NavX system was safe and effective for eliminating persistent/permanent AF.

Objective To study the diagnosis and treatment for the syndrome of inappropriate antidiuretic hormone secretion in neurosurgery.Methods Retrospective analyze clinical situation, treatment, and process of final diagnosis in 6 SIADH cases.Result Diagnosis of SIADH and differential diagnosis with CSWS is very difficult.In clinic the experimental therapy of restricting water and natrium is a important method of differential diagnosis,but also a effective therapy.Conclusion Speciality of SIADH is (1)hyponatremia(Na +≤128 mmol/L),natriuresis(Na +≥80 mmol/24h),(2)ADH assay is no significant for SIADH and CSWS,(3)no hypervolemia and edema exhibition,(4)restrict water is effective in treatment.

Aim To investage the regulatory effect of alizarin on renal interlobar arterial smooth muscle cells. Method The effect of alizarin on BKCa channels was observed by whole-cell patch clamp recording tech-nique. Results Selective BKCa channel blocker ibTX inhibited alizarin-induced outward current(P<0. 05);single smooth muscle cells were incubated in extracel-lular fluid with no Ca2+ 30 minutes, selective L-Ca2+channel blocker nimodipine, selective calcium ion che-lating agent BAPTA-AM and ryanodine inhibited the a-lizarin-induced BKCa channels current, too ( P <0. 05 ) . Conclusion Alizarin relaxes renales interlobar arterial smooth muscle via activation of L-Ca2+ channel firstly, which lead to Ca2+ flowed into cytoplam, and rising of Ca2+ in cytoplam ryanodine receptor indirect-ly, and BKCa is activated at last.

Objective To investigate the correlation between plasma D-dimer with delayed encephalopathy (DE) in patients with acute carbon monoxide poisioning(ACOP).Methods According to the severity of disease,100 patients with ACOP were divided into mild group (27 cases),moderate group (31 cases),severe group (42cases).And depending on whether DE occured,all patients were divided into DE group(20 cases) and no DE group (80 cases).4 venous blood samples were obtained separately from each subject in 1,7,14 days after their admission to hospital by using latex immunoturbidimetry.The levels of blood plasma D-dimer of patients in each group were detected by immune turbidimetry and analyzed,1th,7th and 14th day after admission.Results The plasma D-dimer level of the mild group at different time points was in the normal range,and the difference was not statistically significant(all P ＞ 0.05) ;The plasma D-dimer level of the severe group and moderate group was significantly higher than that of the mild group(all P ＜0.01),and it reached a peak in one week(7 days),and showed a downward trend after two weeks(14 days).The DE all occurred in the moderate and severe group.The plasma D-dimer levels of the DE group were higher than those of no DE group from admission to the 7 th and 14 th day (all P ＜ 0.01).Conclusion The dynamic monitoring plasma D-dimer is helpful in assessing to the severity and prognosis of ACOP,and could surpport positive clinical significance for the prevention and treatment of DE.

Objective To study the clinical value of 18F-FDG PET/CT in childhood neuroblastoma (NB). Methods 18F-FDG PET/CT was performed in 31 children diagnosed with NB. According to the treatment conditions, patients were divided into pre-therapy group, radiation and chemotherapy group, postoperative group, respectively. The positive rate, sites, and im-age characteristics of the primary lesion and metastasis lesion were analyzed through qualitative and quantitative analysis of the image. Results Twenty-one (67.7%) and fourteen (45.2%) patients were found positive in primary sites by CT and PET respectively. All cases (9/9, 100%) in pre-therapy group were found positive in primary lesions by PET, 3 positive cases (75.0%) in radiation and chemotherapy group and 2 positive cases (11.1%) in postoperative group. Twenty-one patients showed metas-tases (67.7%). Lymph nodes (16 cases) and bone (bone marrow) (13 cases) were the most common sites of metastasis followed by pleura, meninges, liver and retrobulbar inifltration, all of which showed increased FDG uptake. Two patients were found lesions in lungs by CT, but had no FDG uptake. SUVmax of primary lesions was signiifcantly different among pre-therapy, chemotherapy and postoperative group (H=13.89, P=0.001), and pre-therapy group had the highest value. Metastases (lymph nodes, bone and bone marrow, pleura, liver and meninges) in pre-therapy group had high SUVmax. Conclusions NB primary tumors are characterized by the increased FDG metabolism. PET can fully detect the distribution of NB metastases in whole body. Except the pulmonary metastasis, metastases in other positions show increased FDG uptake. PET has potential role in evaluating the efifcacy of radiation and chemotherapy, and identifying postoperative residual or recurrence.

Objective To compare the early analgesic effects of continuous femoral nerve block analgesia (CFNB) and patient controlled intravenous analgesia (PCIA) and their effects on functional recovery after total knee arthroplasty.Methods Retrospective analysis was conducted on 60 ASA Ⅰ and ASA Ⅱ patients with unilateral total knee arthroplasty in our hospital from January 2014 to January 2015.The patients were divided into two groups,CFBN group (treatment group) and PCIA group (control group),with 30 patients in each group.VAS scores of postoperative ipsilateral knee resting and movement pain,range of postoperative knee joint motion (ROM),muscle strength of femoral quadriceps,incidence rates of adverse reaction within 7 days after operation,HSS score 3 months before and after the operation,and discharge time were observed.Results Postoperative VIS score in CFNB group was lower than that in PCIA group.ROM and muscle strength of femoral quadriceps in CFBN group were better than those in PCIA group on day 1,2,3,5,and 7 after the operation.Postoperative adverse reaction rate within 7 days in PCIA group was significantly higher than that in CFNB group.HSS score three months after the operation in CFNB group was higher than that in PCIA group.Discharge time of CFNB group was significantly earlier than that of PCIA group (P＜0.05).Conclusion Compared with PCIA analgesia,CFNB analgesia shows better analgesic effect.It can alleviate early knee joint pain after TKA,increase joint motion,significantly shorten the hospitalization time,promote joint exercises which are effective in functional rehabilitation,and decrease the incidence of adverse reactions like nausea and vomiting.