Objective:To establish the experimental nude mouse model of endometriosis for human eutopic endometrium.Methods:Female nude mouse aged 6~8 weeks were selected.Nude mouse in experimental group(n=20)were implanted with the endometrium of patients with endometriosis or without endometriosis into pelvic and abdominal cavities.Nude mouse in control group(n=3)were implanted with the greater omentum.Nude mouse were killed at 5th,15th,and 30th day after implantation for observ ation of the growth of endometriotic lesions.The morphological and histological changes of endometriotic lesions from diferent sources and at diferent time points of growth were examined by light microscopy.Results:The achievement ratio of transplantation was 100%.At the 5th day,endometriotic lesions were found in the pelvic and abdominal cavities,and the adhesion to mice tissues was already tight.There were nascent vascular net between endometriotic lesions and mice mesepithelium.Endometrial glands around with stroma cells were found under light microscope.At the 15th day,endometriotic lesions mixed with mice tissues.Endometriotic lesions presented parenchymatous and cystiform.A lot of endometrial glands and stroma cells with rich blood supply were seen under light microscope.At the 30th day,endometriotic lesions became atrophied,and the adhension was serve partly.Endometriotic lesions presented severe fibrosis and some endometrial cells were not in integrity under light microscope.In the control group,some of the greater omentum adhered to the incisal opening of abdominal wall,while the others were free and atrophied or merged gradually.There was no change in the constructed greater omentum except for some inflammatory reaction.Conclusions:Nude mouse model of endometriosis was good for studying the endometria cell proliferation,neovascularization,hormone,immune response and so on,since the implanted endometrium remain the original morphological and histological features.It is a perfect model of endometriosis because of its significant and steady character,low cost,simple operation and short observation cycle.

Objective To research the effect of nimodipine in the prevention and treatment of delayed facial palsy after microvas-cular decompression .Methods A retrospective analysis was taken from January 2009 to March 2012 ,193 cases of patients with fa-cial spasm undergoing MVD in our department .According to whether applied vasodilators after operation ,all cases were divided into two groups :the experimental group(72 cases)with the treatment of nimodipine for two weeks ;the control group(121 cases) ,and not treated with nimodipine .The follow-up time was 6-12 months .Observe and compare the incidence ,level of delayed facial paraly-sis onset time and disease duration time between two groups .Results In the experimental group had 6 cases of late facial paralysis , the incidence was 8 .3% (6/72) ,onset time was (14 .5 ± 5 .2)days ,disease duration was (41 .3 ± 14 .4)days .The incidence of control group was 9 .1% (11/121) ,onset time was (12 .2 ± 7 .4)days ,disease duration was (55 .7 ± 36 .4)days .There was no significant difference between groups(P>0 .05) .According to House-Brackman classification ,there were 4 cases of grade Ⅱ and 2 cases of grade Ⅲ in experimental group .In control group ,there were 2 cases of grade Ⅱ ,6 cases of grade Ⅲ ,3 cases of grade Ⅳ .The differ-ence was statistically significant (P<0 .05) .Conclusion The delayed facial paralysis is influenced by a variety of causes .Vasodila-tor drugs can reduce the incidence of delayed facial paralysis ,and play a certain role in promoting facial paralysis restored .

AIM:To compare the effect of recombinant human epidermal growth factor eye drops and deproteinized calf blood extract eye drops on corneal edema after phacoemulsification.METHODS:Totally 72 cases (72 eyes) of patients undergoing phacoemulsification were selected and divided into the observation group and the control group by random number table method.After surgery,the observation group were treated with deproteinized calf blood extract eye drops while the control group were treated with recombinant human epidermal growth factor eye drops.The degree of corneal edema,subjective symptom score,corneal endothelium count,changes of corneal thickness and postoperative visual acuity recovery were compared between the two groups at different time points after surgery.RESULTS:Corneal edema in the two groups was significantly milder at 1wk after surgery than that on the 1st day after surgery (P<0.001).There was no significant difference in the degree of corneal edema and test results of visual acuity between the two groups at 1wk after surgery (P>0.05).Compared with 1d after surgery,the subjective symptom score and corneal thickness of the two groups significantly decreased on the 7th day after surgery (P<0.05).At 7d after surgery,the above indexes were significantly better in the observation group than the control group(P<0.05).At 1mo after surgery,the corneal endothelium count was significantly larger in the observation group than the control group (P<0.001).CONCLUSION:Both of recombinant human epidermal growth factor eye drops and deproteinized calf blood extract eye drops can significantly relieve corneal edema and improve visual acuity of patients after phacoemulsification.However,the latter has obvious advantages over the former in the repair of corneal endothelial cell injury after surgery.

This paper was to explore the application of case based learning method in medical psychology course for medical undergraduates.According to curriculum standard,nine cases were compiled which matched six articles,forming the complete instructional design.We followed the five steps of scientific teaching stage.The preliminary results showed that the case teaching was helpful to improving the medical students' knowledge acquisition,and their ability to master and solve practical problems.

Pharmacogenomics aims to identify the effects of genomie variants on drug efficacy and safety. This article summarized the progress of pharmacogenomic study on anti-diabetic drugs.

MicroRNA(miR),a type of 22nt non-coding RNA,modulate gene expression at posttranscriptional level by interacting with targeting mRNA and play an important role in both inflammatory reaction and autoimmune diseases.Especially,the expression of miR-146a in autoimmune diseases has gotten more and more attention in recent studies.This review summarized the correlation between the expression of miR-146a and the pathogenesis of some common autoimmune diseases.What's more,the potential role of miR-146a in the clinical diagnosis of autoimmune diseases are also discussed.

Objective To investigate the effects of intravenous injection of α-crystallin on retinal ganglion cells (RGC) and some important organs of the Long Evans rats. Methods RGC were retrogradelabeled by fluorogold through bilateral superior colliculus and lateral geniculate body for seven days before optic nerve crush injury. Twenty-three Long Evans rats were used for this study, including three rats of normal control group and 20 rats of experimental group. Twenty rats were randomly divided into saline control group and three α-crystallin injection groups, which received tail vein injection of 1.25 ml isotonic saline and three different concentrations (1 × 10-2 , 1 × 10-1 and 1 g/L) of α-crystallin respectively, once every two days and totally seven times. After two weeks, the labeled RGC were counted, and the pathological changes on liver, kidney, brain, spleen and the lungs were investigated. Results Compared with the normal control group, although the number of RGC markedly decreased after two weeks of optic nerve crush injury in every group, the number of RGC in α-crystallin-treated groups was more than those in the saline control group. There were 2074± 150 RGC per mm2 in normal control group, 85 ± 15 RGC per mm2 in saline control group, 124±26 RGC per mm2 in 1 × 10-2 g/L α-crystallin group, 128± 31 RGC per mm2 in 1 × 10-1 g/L α-crystallin group, 164 ± 20 RGC per mm2 in 1 g/L α-crystallin group (F= 18. 660,P＜0. 01). No congestion, swelling, inflammation and other pathological changes were found in liver,kidney, brain, spleen and lung. Conclusions Intravenous injection of α-crystallin protein has protective effects on RGC after the optic nerve crush injury, and no significant effects on important organs.

Psychological problems interrupt the study and life of overseas students, and even lead to a crisis. Based on a study on the results of questionnaires for undergraduate overseas students' on-campus lives in Chongqing Medical Universities,solitude,frustration and depression,the three most popular psychological problems of overseas students in medical universities are analyzed and countermeasures are put forward.

Objective To determine the respiratory depression effect-site concentration (Ce) of remifentanil when used in combination with different target concentrations of propofol in patients undergoing neurosurgical surgery. Method Eighty patients aged 18-64 yr weighing 45-90 kg scheduled for elective neurosurgical surgery were randomly divided into 4 groups (n = 20 each): remifentanil group (group R), remifentanil combined with propefol 1, 1.5, 2 μg/ml group (group RP1, RP1.5, RP2). In group R, RP1, RP1.5 or RP2, the patients received propofol by TCI at target plasma concentration (Cp) of 0, 1, 1.5 or 2 μg/ml respectively, when the preset concentration was reached, received remifentanil via TCI at the initial target Cp of 2 ng/ml, and the concentration was then increased in the increment of 2 ng/ml at 3 min intervals until respiratory depression was achieved. Respiratory depression was defined as one of the following end points: muscle rigidity, RR<6 bpm, MV < 3 L/min, PETCO_2> 55 mm Hg, SpO_2 < 90% or apnea > 15 s. The Ce of remifentanil, amount of remifentanil used and side effects were recorded during respiratory depression. Result The respiratory depression Ce of remifentanil were (5.2±2.1), (3.2±1.0), (2.9 ±1.3) and (2.2±1.0) ng/ml in group R, RP1, RP1,5 and RP2 respectively. Compared with group R, the respiratory depression Ce of remifentanil were significantly decreased in group RP1, RP1.5 and RP2 (P<0.01). Compared with group RP1 and RP1.5, the respiratory depression Ce of remifentanil was significantly decreased in group RP2(P<0.01). There was no significant difference in respiratory depression Ce of remifentanil between group RP1 and RP1.5 (P > 0.05). Conclusion The respiratory depression Ce of remifentanil is (5.2±2.1) μg/ml in patients without sedation. The respiratory depression Ces of remifentanil are (3.2 ±1.0), (2.9 ± 1.3) and (2.2 ±1.0) ng/ml in patients under sedation with propofoi at target Cps of 1, 1.5, 2 μg/ml during neurosurgical surgery.

Being one of the commonly used method of external treatment,fumigate treatment has been exist for thousands of years in China.It has been generally used for its certain therapeutic effect and simple manipulation.But the studies on basic theory of this therapy were few.In this article,the connotation of the fumigate treatment and the theory of meridian skin areas were expounded,and the relation between fumigate treatment and meridian skin areas were also fully discussed.