Objective To explore the expressions and significances of sICAM-1 and sCD44v6 in peritoneal lavage fluid after laparoscopically assisted radical gastrectomy,in order to evaluate the risk of laparoscopically assisted radical gastrectomy for the peritoneal plant transfer.Methods Ninety-two patients with gastric cancer were randomly divided into the control group and the experimental group.The control group was treated with open laparotomy and the experimental group was treated with laparoscopic surgery.The concentrations of both sICAM-1 and sCD44v6 were detected by enzyme linked immunosorbent assay.The positive rates of tumor cells in the two groups were compared.Results The expressions of sICAM-1,sCD44v6 in the two groups before operation had no significant difference (t =0.198,P =0.543 ; t =0.897,P =0.429).After operation,sICAM-1 and sCD44v6 levels increased significantly,but there was no significant difference between the two groups (t =0.766,P =0.312 ; t =1.092,P =0.129).The positive rates of tumor cells in the two groups before and after operation have no significant differences (x2 =0.789,P =0.287 ; x2 =0.912,P =0.198).Conclusion Laparoscopically assisted radical gastrectomy does not increase the expression of sICAM-1 and sCD44v6 in peritoneal lavage fluid and the risk of peritoneal metastasis.It is worthy of clinical poptdarization and application.

Objective To investigate the clinical efficacy of cobamamide combined with Longxuejie in the treatment of herpes zoster in the elderly .Methods 104 elderly patients with herpes zoster were randomly divided into the treatment group and the control group .Both the two groups were given orally famciclovir 250mg,3 times a day for 14 days,intramuscularly injected gland glycosides cobalamin 500mg,1 day,14 days a course of treatment ,the treat-ment group was given Longxuejie enteric-coated tablets 4,3 times a day orally for 14 days.The blisters disappeared , pain relief,and recovery time of completely crusted ,treatment efficacy and neuralgia were compared between the two groups.Results In the treatment group,34 cases were cured,effective in 13 cases,total effective rate was 90.4%, which was significantly higher than 73.1% in the control group,the difference was statistically significant (χ2 =5.216,P<0.05).The blisters disappeared,pain relief,and recovery time of completely crusted in the treatment group were significantly shorter than those in the control group (t =3.216,5.432,4.643,3.654,all P <0.05).No postherpetic neuralgia was observed in the treatment group ,the incidence rate of postherpetic neuralgia of the control group was 13.5%,the difference was statistically significant (χ2 =4.875,P<0.05).Conclusion The Longxuejie combined with cobamamide in the treatment of herpes zoster in elderly patients has reliable effect ,fast improvement of clinical symptoms,a low incidence of postherpetic neuralgia ,it is worthy of clinical application .

Objective To observe the auto-limbal epithelium and amniotic membrance transplantation in treatment of Symblepharon.Methods Transplant with auto-limbal epithelium and aminiotie membrance including 23 patients (31eyes) some of them are recurrentcd pterygium,most of them are symblepharons.[ Result ] In the followup period for 3～12 months,Ocular surface reconstruction was successful in 26 of 31 eyes with symb|epharon,but five of them recurrented three months later with lees than pre-transplantation in degree.Conclusion The transplantation with auto-limbal epithelium and membrance transplantation is an effective method for the symblepharon.

10 kinds of normal and immunodifi-cient mice were used and they were found to have different levels ofNK activity.The NK activity toHuman to Human,Human to Mouseand Mouse to Mouse hybridomacells was much lower thanthat to YAC-1 cells in all mice andthere were no significant differencesbetween the two kinds of human Bcell hybridomas in susceptibility toNK cells.The results showed thatNK cells had no obvious effects onxenografts of human B cell hybri-domas.

The red blood cell sorbitol levels of 23 healthy subjects and 66 diabetic patients were measured enzymatically. Results showed that erythrocyte sorbitol levels of both IDDM (17) and NIDDM (38) patients with poor control were significantly higher than that of the controls and positively correlated with the coincident plasma glucose concentration, 24-hour urinary glucose excretion and HbA1c. There was no significant difference between the red cell sorbitol concentration of NIDDM with normal blood glucose level and that of the normal group; moreover, red cell sorbitol levels did not sig-nificantly correlated with FBG in both groups. Although there was no significant difference between the FBG of poorly controlled IDDM and NIDDM groups, red blood cell sorbitol levels were significantly different in both groups. This suggested that red blood cell sorbitol level could be used as an indicator of diabetic control.

The present paper is an analysis of the pattern reversal visual evoked potentials (P-VEP) of macular disease (40 cases, 57 eyes), in contrast to normal persons (40 cases, 80 eyes) of similar age. The group consists of 9 different kinds of disease-central serous chorioretinopathy macular hemorrhage, macular dege-neration, etc. The authors analyzed the latency and amplitude of P-VEP in different macular disease with different course and central vision. The results showed that the latency of P-VEP in the group was relatively prolonged. In contrast to normal person P 100 was prolonged by 9.0, 26, 40 ms at 1?2′, 30′, 16′ visual angles respectively. The amplitude was obviously lowered, especially at the visual angle of 16′ and P-VEP extinguished in all the eases with visual acuity less than 0.1.The latency and the amp-litude were not related to the type and the course of the maeular disease, but were closely related to the central vision.

OBJECTIVE:To enhancement lawconsciousness and ability of self-protect of pharmacist.METHODS:Analysed the problem which occured in their work from the point of view of law and bring forward some advice.RESULTS &CONCLUSION:Pharmacist should do their work strictly according to the code for avoidance law risk.

Objective To study the effect of acid burn on rat corneal endothelial damage and its repair.Methods The model of mild,moderate and severe acid burn in cornea was established in 90 Long-Evans rats(n=30 for each degree of acid burn) with 4 rats served as normal controls.Corneal edema,opacity and the healing of epithelium were observed with a slit lamp microscope.The corneal endothelium from half rats of each degree acid burn was stained with trypan blue and alizarin red,and that from the other half with hematoxylin and eosin(HE) immediately,30 min,1,3,7,14,21,28 d after cautery.The endothelial damage and its repair were observed and the number of the leucocytes in cornea was calculated under light microscope.Results No damage was seen in the corneal endothelial cells of mild,moderate acid burn,only a few leucocytes could be seen in the cornea,and the healing of epithelium,the regression of edema were within 7 d.The endothelial cells necrosed and shedded in the wound area by severe acid burn.The endothelial cells in the margin were deformed,elongated and slided to the damaged area during repair,and a great number of leucocytes infiltrated,the healing of corneal epithelium was delayed,the regression of edema was up to 28 d.Conclusion The corneal endothelial cells were damaged in severe acid burn.The secondary inflammatory reaction took part in the damage of corneal endothelial cells after severe acid burn.The damage of endothelial cells delayed the healing of corneal ulcer and edema.

Objective To compare the sensitivity of body mass index (BMI),waist-hip ratio (WHR)and percentage body fat (PBF)in the evaluation of the obese adolescents population,and find a more sensitive evaluation method.Methods In this study,2 045 adolescents were recruited in the city of Chongqing by using multiple-stage random cluster sampling,and they were identified as overweight or obesity on the base of BMI,WHR and PBF.Results There was significant difference among the detection rate of the three methods (P<0.05),the detection rate of PBF was the highest (12.9%),then BMI (7.2%)and WHR (6.0%).consistency test showed that the concordance of three methods was poor and Kappa (BMI and WHR)=0.199,Kappa (BMI and PBF )=0.441,Kappa (WHR and PBF)=0.187;comprehensive evaluation was chosen as the gold standard and compared with three meth-ods respectively by ROC curve,the analysis showed that area under curve (AVC):PBF(0.954)>BMI(0.918)>WHR (0.694). Conclusion The sensitivity of PBF and BMI was higher.Although the WHR sensitivity was lower,WHR is a good predictor asso-ciated for chronic diseases,which suggests that the development of appropriate adolescent WHR standard is necessary.

AIM: To study the mechanism and protective effect of rosmarinic acid from Rosmarinus officinalis extract on acute hepatic injury induced by carbon tetrachloride in mice. METHODS: Mouse model of acute hepatic injury was set up by giving CCl_4 abdominally.The effects on ALT,AST avtivities,TG level in serum and MDA level in liver tissue were examined.Histological change and pathological change were observed by means of hematoxylin and eosin staining.The body weight,liver weight of each mice were measured. RESULTS: Rosmarinic acid significantly reduced the activities of ALT,AST,level of TG in serum and level of MDA in liver tissue,restrained the enlargement of liver and reduced the necrosis in pathological observation. CONCLUSION: Rosmarinic acid shows significant inhibitory effects on liver injury induced by CCl_4 in mice.The mechanisms may be related to its effects of reducing lipid peroxidation product.