Objective　To observe the presence of synergistic efficacy between adenovirus mediated bcl-Xs (Adv-bcl-Xs) gene therapy and chemotherapy on ovarian cancer cell. Methods　NuTu-19 cells were infected by different titers of Adv-bcl-Xs and were treated with topotecan at the same time. Cell proliferation was measured 3 days later by MTT. Graphical representations of the statistical analyses recorded their interaction in tumor cells. Results　The statistical results and graphical representations of the statistical modeling showed that the synergistic antiproliferative activity was present (P＜0.01). Conclusion　There were synergistic efficacies between Adv-bcl-Xs gene therapy and Topotecan on ovarian cancer cell.

Objective To discuss the treatment effect of doctor-nurse cooperation in multi-plane operation of obstructive sleep apnea hypopnea syndrome.Methods 73 patients with multi-planar surgery due to OSAHS were divided into the observation group (38 cases) and the control group(35 cases) in our department from January 2008 to December 2012.Health care was strengthened throughout the course of treatment in the observation group and traditional treatment and care were adopted in the control group.Short-term and long-term efficacy,intraoperative and postoperative complications were compared between two groups.Results The operation of short-term effective rate was 100％,and significant differences were found in the long-term efficacy between the two groups.There were significant differences in nasal bleeding during surgery,one-time intubation success rate and the significances were found in postoperative pain,extubation time,lung infection and soft palate regurgitation between two groups.Conclusions The implementation of the multi-plane surgery for patients with OSAHS can effectively improve the short-term efficacy of the patients throughout the course of treatment.Strengthening doctor-nurse cooperation effectively improve the patients' long-term efficacy,reducing the patients' intraoperative and postoperative complications.

Objective To observe the effect of Tongsaimai Tablets on the leptomeningeal collateral circulation established after acute cerebral infarction by TCD.Methods 50 patients with acute cerebral infarction were randomly divided into control group (received conventional treatment) and treatment group (received Tongsainai Tablets three times a day,1.75g each time,on the basis of conventional treatment).The leptomeningeal collateral circulation of the two groups were observed after 6 months.Results The factors which may affect the leptomeningeal collateral circulation,such as age,the prevalence of hypertension and hyperlipidemia,original compensatory status of cerebral artery between the two groups had no statistically significant differences.The open rate of leptomeningeal collateral circulation in the treatment group was 64.00％,which was significantly higher than that of the control group(32.00％)(P =0.024).Conclusion Tongsaimai Tablets can promote the formation of leptomeningeal collateral circulation in patients with acute cerebral infarction.

Phosphatase of regenerating liver-3 (PRL-3) is a novel small molecule protein-tyrosine-phosphatase,which plays an important role in the oncogenesis and deveopment of tumors.Studies show that PRL-3 regulates neoplasm progress through participating in multiple signal pathways.Its high expression can significantly promote neoplasm metastasis in cancer tissue.However,there is no expression of PRL-3 in most normal tissue.PRL-3 is expected to be a potential tumor maker of diagnosis and a new target for the therapy of cancer.

Objective To observe if IL-2 gene can express in hepatic carcinoma cells by double targeting of hepatitis B virus envelope (HBVE) and alpha fetoprotein (AFP) promoter.Methods HepG2 cells,L02 cells,and HepG2.2.15 cells were cultured in vitro.HBVE was obtained by PEG8000 concentration assay,and the acquired HBVE was used to pack recombintional gene.Human AFP promoter-IL-2 recombinational gene was obtained by PCR.Then HepG2 cells and L02 cells were transfected by transient transfection and the expression of IL-2 was detected by RT-PCR and Western blot.Results IL-2 was detected in HepG2 cells by RT-PCR and Western blot but not in L02 cells.Conclusion By using HBVE as a gene transporter,human AFP promoter-IL-2 recombinational gene can express in hepatic carcinoma cells,thereby it can increase the safety of exogenous gene transfection of hepatic carcinoma cells.

The incidence of upper limb dysfunction of stroke patients was high, and the recovery was difficult, which seriously impact-ed their activities of daily living and quality of life. This article reviewed the new techniques and methods for hemiplegic patients after stroke. The main techniques and methods includeed bilateral isokine-matic training, mirror neuron system, gymnastics rods training, music therapy, motor imagery, upper limb rehabilitation robot, virtual reality, constraint induced movement therapy, functional electrostimulation, electromyographic biofeedback therapy, transcranial magnetic stimulation, neurophyisological therapy and so on.

Objective To observe the evolution process of cerebral blood flow spectrum in brain death by transcranial doppler ultrasound.Methods To detect the blood flow of bilateral middle cerebral artery in brain dead patients by transcranial doppler ultrasound.Results Twenty brain dead patients were collected in the study.The blood flow velocity of middle cerebral artery ranged s from 6 to 37cm/s,and the mean velocity was (12.13 ± 3.15)cm/s.There were three characteristic spectrums which include oscillatory wave (reverse wave in diastolic),nails wave (tip small wave in systolic),no blood flow sign,and they appeared in turn accoerding to worsen of the disease.The heartbeat irreversibly stopped within 8 days after the appearing of oscillatory wave,and ninty percent of the cases had the regular blood flow spectrums of oscillatory wave-nails wave-no blood flow sign.Conclusion The three regularly characteristic transcranial doppler ultrasound blood flow spectrums,which include oscillatory wave,nails wave and no blood flow sign could facilitate the diagnosis of brain death.

ObjectiveTo explore the scientific and effective management of subsidization for students in poverty and to build a rational, scientific-supporting system of poor students. MethodsAnalyzing the data of the poverty status and funding, of poor students in 2006-2009 in an independent Institute ResultsAmong all the means of subsidization for students in poverty, study aid covers most and the number of students who get this aid is increasing while there are fewer national scholarships and living subsidies. ConclusionSupporting system of poor students is not perfect enough, and institutions of higher learning should find the practical way to help our poor students complete their studies successfully.

OBJECTIVE: To study the classification of common symptoms and the laws of syndrome element combination in 251 cases of stable angina pectoris (SAP) by using cluster analysis and corresponding-correlation analysis. METHODS: A total of 251 SAP cases were selected and their information from four diagnosis in traditional Chinese medicine was recorded. The classification of common symptom and the laws of syndrome element combination were investigated by cluster analysis and corresponding-correlation analysis. RESULTS: Twenty-five symptoms found in 251 SAP cases were divided into four types by cluster analysis: deficiency of heart qi, deficiency of spleen qi, deficiency of qi and yin, and phlegm accumulation and blood stasis. The deficiency of heart qi had the closest relation to phlegm accumulation and blood stasis. By corresponding-correlation analysis, the deficiency of qi had the closest relation to blood stasis, next was turbid phlegm and heat stagnation, and then deficiency of qi and deficiency of yin. CONCLUSION: Blood stasis due to deficient qi is the key factor in pathogenesis of SAP. Deficiency of qi plus blood stasis, deficiency of qi plus deficiency of yin, blood stasis plus turbid phlegm, deficiency of heart qi plus blood stasis plus turbid phlegm are common syndrome element combinations of SAP. It is proved that cluster analysis and corresponding-correlation analysis are the proper methods for studying laws of syndrome element combination.

Objective To assess the predictive value of cerebral arteriosclerosis to acute cerebral infarction evaluated by transcranial doppler ultrasound .Methods 170 cases suspected cerebral arteriosclerosis consecutively enrolled were given transcranial doppler ultrasound and were followed up for 6 months,then we assessed the correla-tion between the levels of cerebral arteriosclerosis and acute cerebral infarction within 6 months.Results By Spearman rank correlation analysis ,there was positive correlation between the severity of cerebral arteriosclerosis de-tected by transcranial doppler ultrasound and acute cerebral infarction within 6 months,the correlation coefficient was 0.575(P<0.05).Conclusion Through the detection of transcranial doppler ultrasound ,the higher levels of cere-bral arteriosclerosis,the greater probability of acute cerebral infarction within 6 months.