BACKGROUND:The repair effects of bone marrow mesenchymal stem celltransplantation on brain injury were not ideal. Combined therapy with medicine and biological engineering materials is needed. OBJECTIVE:To investigate the effects of bone marrow mesenchymal stem celltransplantation and fingolimod immunosuppressants on memory function recovery in rats with brain injury. METHODS:A total of 60 healthy Sprague-Dawley rats were subjected to hydraulic shock with peak value of 253.312 5-303.975 kPa with a hydraulic head injury instrument so as to induce a model of severe hydraulic head injury. They were randomly divided into brain injury group, bone marrow mesenchymal stem celltransplantation group and fingolimod+bone marrow mesenchymal stem celltransplantation group. The Morris water maze test was tested at 21-28 days after PKH-26-labeled bone marrow mesenchymal stem celltransplantation. The PKH-26 immunofluorescence and hematoxylin-eosin staining were conducted in brain tissues at 4 weeks after brain injury. RESULTS AND CONCLUSION:At 4 weeks after transplantation, the average escape latency was gradual y decreased in each group. The average escape latency was shorter in the fingolimod+bone marrow mesenchymal stem celltransplantation group than in the bone marrow mesenchymal stem celltransplantation group (P<0.05), and significantly shorter than in the brain injury group (P<0.01). The number of times of crossing the platform and the percentage of swimming distance to total distance were higher in the fingolimod+bone marrow mesenchymal stem celltransplantation group than in the brain injury group and bone marrow mesenchymal stem celltransplantation group (P<0.05). The number of PKH-26-positive cells was significantly higher in the fingolimod+bone marrow mesenchymal stem celltransplantation group than in the brain injury group and bone marrow mesenchymal stem celltransplantation group (P<0.05). Results confirmed that bone marrow mesenchymal stem celltransplantation could apparently improve memory function of rats with severe brain injury. The combined application of fingolimod immunosuppressants has synergistic effects.

Objective To explore the related high risk factors of hyponatremia after transsphenoidal approach for pituitary adenomectomy and to direct postoperative nursing. Methods The clinical data of seventy-seven patients suffering from pituitary adenomas with the way of treatment by transsphenoidal pituitary adenomectomy from June 2014 to May 2015 were summarized retrospectively. According to the value of postoperative serum natremia, the data were divided into hyponatremia group and non-hyponatremia group. The different data between the 2 groups were compared and the high risk factors related to hyponatremia were analyzed. Results Univariate analysis showed that gender(P=0.254) and high blood pressure(P=0.742) were unrelated to postoperative hyponatremia and there was significant difference between two groups in age (P=0.038), max- diameter of tumors(P=0.004), preoperative pituitary dysfunction (P=0.013), visual deficit (P=0.000), operative procedure duration (P=0.008) and diabetes mellitus rate (P=0.023). While Logistic regression analysis showed that preoperative visual deficit (OR=0.152,P=0.004,95%CI 0.043-0.542) and preoperative pituitary dysfunction (OR=0.069, P=0.046,95%CI 0.005-0.950) were independent factors for postoperative hyponatremia. Conclusions Hyponatremia is a common complication after transsphenoidal pituitary adenomectomy. The optimal treatment can benefit the early recovery and more careful nursing should be focused on these patients who are in high risk of postoperative hyponatremia.

Extracellular vesicles (EV) miRNAs are a class of small non-coding RNAs that are encapsulated in membrane-containing vesicles from a variety of cells, and which can mediate intercellular communication and transfer of functional molecules. EV miRNAs in circulation can be served as novel molecular markers for cancer diagnosis, monitoring and prognosis, and also the most promising liquid biomarkers for clinical translation and utility currently, while optimal research strategy is crucial for identifying valuable EV miRNAs in clinical study. Moreover, EV miRNAs have recently also been implicated in cancer progression as direct regulators of metabolic reprogramming, mesenchymal-to-epithelial transition, angiogenesis, anti-cancer immune response and drug resistance in the tumor microenvironment. Study on some key EV miRNAs involving in those processes may highlight their potential utility as liquid biopsies in early diagnosis, companion diagnosis, metastasis, therapeutic control, prognosis analysis as their therapeutic potential for patients with cancer.

Purpose To evaluate the diagnostic efficiency of spectral CT imaging combined with oral isotonic mannitol gastrointestinal hypotonic radiography in the diagnosis of preoperative T and N staging of rectal carcinoma. Materials and Methods Forty-seven patients with rectal cancer confirmed by surgery and pathology were included in this study, all the subjects underwent oral isotonic mannitol gastrointestinal hypotonic radiography prior to CT scan, then abdominal and pelvic plain and double-phase contrast-enhanced CT scan in the spectral imaging mode was performed, the optimal contrast-to-noise ratio imaging were got by the spectrum analysis software, then T-stage was evaluated and compared with pathological T-stage; N-stage was evaluated using both traditional method and the spectral curve method, and compared with pathological N-stage. Results ① All the 47 patients were able to successfully complete the examination and the rectal filling and lesion display were satisfactory; ② the best diagnostic accuracy of T-stage by the monochromatic images was 83.0% (39/47), which had a general correlation with pathologic results (Kappa=0.713, P<0.001); ③ the accuracy, sensitivity, specificity, positive predictive value and negative predictive value of N-stage by the traditional method were 78.72%, 80.95%, 76.92%, 73.91% and 83.33%, respectively, which had a general correlation with pathologic results (Kappa=0.574, P<0.001); the accuracy, sensitivity, specificity, positive predictive value and negative predictive value of N-stage by the Spectral curve method were 89.13%, 75.00%, 100.00%, 100.00% and 83.87%, respectively, which had a fair correlation with pathologic results (Kappa=0.772, P<0.001). Spectral curve method had higher Youden index for N-stage evaluation than traditional method (0.64 vs. 0.58). Conclusion Oral isotonic mannitol gastrointestinal hypotonic radiography can provide a good rectal background, and it is a safe and reliable method for rectal examination, when combined with spectrum CT imaging, it is able to display the detail of lesions and identity the homology of lymph nodes, which might help to make more accurate preoperative T, N staging of rectal carcinoma.

Autonomic nervous system(ANS) activity plays an important role in the pathogenesis of cardiovascular disease,including organic cardiovascular disease(such as hypertension,coronary artery disease,etc) and functional cardiovascular disease (such as vasovagal syncope,postural orthostatic tachycardia syndrome,etc).Many methods was used to evaluate ANS function.Heart rate variability is a widely accepted method to assess cardiac autonomic nerve function.Autonomic nervous affected cardiac action potential and let electrocardiogram changes.Through the sympathetic and parasympathetic neurotransmitters which acting on the corresponding receptors.Electrocardiogram changes reflects autonomic nervous function.Present researches suggest that electrocardiogram has an important significance in the evaluation of autonomic nerve function in functional cardiovascular disease.

Objective By comparing the prevalence of mental disorders and social demographic characteristics, to analyze the influencing factors of mental disorders in Xiamen city residents. Method Using multi-stage stratified cluster random sampling, 12 071 subjects aged 18 years and older were identified in Xiamen city. Eventually 10 764 subjects completed the survey, including 4 957 males, 5 807 females, 8 588 urban population and 2 176 rural population. The subjects were screened with the expanded version of GHQ-12, the Structured Clinical Interview for DSM-Ⅳ-TR as a diagnostic tool, and made a determination whether they had mental disorder and specific diagnosis. Result The overall prevalence of mental disorders in Xiamen city was lower than that in other provinces and cities; it was only 3.46%(Shandong province 19.48%, Zhejiang province 17.27%, Qinghai province 18.04%, Hebei province 16.24%, Tianshui city 17.90%, Chengde city 17.71%, Baoding city 13.62%). The one of most common mental disorders was the alcohol use disorders in various provinces and cities, except in Xiamen city. The constituent ratio of Xiamen residents surveyed was 79.78%for urban, higher than those of other provinces and cities (Shandong province 24.91%,χ2=6 624.955,P<0.001;Zhejiang province 32.95%,χ2=3 601.752, P<0.001;Qinghai province 23.55%,χ2=4 991.289,P<0.001;Hebei province 15.95%,χ2=11 547.016,P<0.001;Tianshui city 16.05%,χ2=6 484.483,P<0.001;Chengde city 15.60%,χ2=2 930.811,P<0.001;Baoding city 11.80%,χ2=6 973.770,P<0.001). Most residents of Xiamen enjoyed at least one kind of health insurance (83.06%), higher than those of other provinces and cities (Zhejiang province 23.30%,χ2=8 868.617,P<0.001;Hebei province 8.68%,χ2=17 434.576,P<0.001;Tianshui city 11.20%,χ2=10 862.305, P<0.001;Chengde city 8.69%,χ2=5 884.478,P<0.001;Baoding city 9.54%,χ2=10 728.632,P<0.001). Conclusion The overall current prevalence of mental disorders in Xiamen city residents was lower than those of other provinces and cities. The reasons may be that there was higher degree of urbanization, better health care, no heavy drinking habits and other factors. There might be other protective factors which need to be further studied.

Objective:To examine postural control between normal and attention deficit hyperactivity disorder(ADHD)boys,and compare the development characters.Methods:In the study,146 ADHD boys and 84 normal boys participated,ages being between 7 and 12 years.Balance Master was used to assess posture control.Posture stabilities were tested under 4 different conditions(firm surface with eyes open,firm surface with eyes closed,foam pad with eyes open,foam pad with eyes closed).We tested three times for one condition,20 s per time.The mean sway velocity was recorded.Results:(1)Normal boys:In firm surface with eyes open,analyses of variance between normal boys revealed no significant differences between different age groups,and the 11-and 12-year-old group did not achieve scores comparable to the adult(t=6.145,P

Objective To summarize the experience on bronchiallavage to cure pulmonary atelectasis and pulmonary infection after thoracotomy.Methods 48 patients were selected and treated by bronchiallavage under monitoring,and antibiotics was used according to sputum culture and drug sensitive test.The out come was analyzed and evaluated.Results All patients of the pulmonary atelectasis were cured and those with pulmonary infection were controled.There was no serious complication.Conclusion Bronchiallavage is applied to treat pulmonary atelectasis and pulmonary infection after thoracotomy,the curative rate is significant.The appliance of antibiotics is guided by drug sensitivity.It is such a safe and feasible therapeutic tool that the bronchofibroscope is applied to cure pulmonary atelectasis and pulmonary infection after thoracotomy.

Objective Covered stents have been widely utilized in aortic dissections and aortic aneurysms since Parieli treated an abdominal aortic aneurysm with a stent-graft in 1991.Covered stents have also been reported for managing peripheral vascular diseases,including large or wide-neck aneurysm,pseudoaneurysm,vascular rupture/perforation and arteriovenous fistula.Encouraging achievements are also bringing in more than one decade,even in China.For transjugular intrahepatic portosystemic shunt creation,the primary patency rate at 1 year after covered stent grafting is statistically longer than that of bare stent.At present,it is worth studying randomly the covered stent versus bare stent in treating long segmental arterial stenosis and/or occlusion.Furthermore observation should be insisted upon for the long term patency of parent artery after implanting with covered stent.Deploying a covered stent into a tortuous vessel is still a challenge and tough problem.There would still be a long way in China for further development in basic research of improving the substantial property of stent-graft and better design of deploying device.The main target for all interventional radiologists in the foreseen future is how to develop new covered stents with independent knowledge property right and to expand new horizon for its application.

Objective To investigate the correlation between interleukin 18 (IL-18) gene promoter -137 G/C polymorphism and plasma levels of IL-18 and cerebral infraction.Methods A total of 218 Han patients with acute cerebral infarction in northern China were recruited and 218 sex- and age-matched health subjects were used as control group. Enzyme-linked irnmunosorbent assay was used to detcet the level of IL-18. Sequence specific primer-polymerase chain reaction was used to detect IL-18 gene promoter - 137 G/C polymorphism. Results The plasma IL-18 levels (1 47. 47 ±29. 79 ng/L vs. 78. 78 ± 16. 42 ng/L; t =29. 816, P ＜0. 01)and the frequency of - 137 GG genotype (79. 8％ vs. 67. 0％;x2 =8. 563, P =0. 032) in the acute cerebral infarction group were significantly higher than those in the control group. The risk of cerebral infarction in individuals with - 137GG genotype was 1.95 times higher than that in individuals without - 137GG genotype (odds ratio 1.95, 95％ confidence interval 1. 263-3. 012,P =0. 002); plasma IL-18 levels in patients with CG genotype were significantly higher than those in patients with CG +CC genotype (150. 00 ±27. 26 ng/L vs. 118. 47 ±21.62 ng/L, t =8. 222, P ＜0. 01). Conclusions The IL-18 gene promoter - 137 G/C polymorphism correlates with cerebral infarction in Han population in north China. The risk of cerebral infarction in individuals with GG genotype is significantly higher, and it may be associated with its influence on plasma level of IL-18.