MicroRNAs ( miRNAs) play an important role in regulating various life activities .It has been confirmed that circulat-ing miRNAs are new biomarkers for the diagnosis of many diseases and closely related with their occurrence and progression .In recent years, miRNAs are found to exist stably in blood and other body fluids in forms of being encapsulated in microvesicle or binding with proteins.Their origins, existence forms and functions have become a hotspot of research in this field .This article reviews the recent studies on the origin , existence form and function of circulating miRNAs associated with cardiovascular disease , aiming to provide some evidence for miRNAs as the biomarker and target of cardiovascular disease .

Acupuncture Therapy ; Adult ; Humans ; Male ; Ossification, Heterotopic ; therapy ; Temporal Bone ; abnormalities

The clinical application of acupoints in Huangdi Neijing was analyze by evidence-based medicine. The results showed that 202 acupoints were recorded,the clinical usage rate was 81.28%,the first 10 acupoints with highest clinical usage rate were Yangming of foot,Taiyin of foot,Shaoyin of foot,Yangming of hand,ST36,Taiyin of hand,Taiyang of foot,shaoyang of foot,BL40,Taiyang of hand. But meridian acupoints with highest clinical usage rate were ST36,BL40,KI2,KI1,ST45,BL10,LR1,ST42,SP3 and PC9.

Objective To study the clinical application of posterior approach microendoscopy in the treatment of lumbar spinal stenosis. Methods One hundred and forty-two cases of degenerate lumbar spinal stenosis were treated by posterior approach limited decompression, total or semi laminectomy and decompression, or fenestration decompression from February 2000 to December 2001. Results Out of the 142 cases, posterior approach microendoscopic limited decompression was completed in 87 cases with full decompression obtained.Follow-up for a mean of 18 months in 82 cases revealed a rate of excellent-and-good of 92.7% (58 cases, excellent; 18 cases, good).No complications occurred. Conclusions Degenerate lumbar spinal stenosis, resulted from simple lumbar intervertebral disc herniation, hypertrophy of ligamentum flavumcan, and facet hypertrophy, can be effectively treated by posterior approach microendoscopic limited decompression. Proper selection of patients, full decompression and experienced operative skills are the key to satisfactory surgical results.

Objective To analyze inhibitions and working memory impairments in children with different subtypes of attention deficit hyperactivity disorder (ADHD) with saccade tasks. Methods Participants,aged 10～ 13 years with ADHD-combined ( n= 19) ,ADHD-inattentive ( n= 11 ) and control subjects ( n= 18) ,were selected according to DSM-Ⅳ diagnosis,Conners teacher rating scale and parent symptom questionnaire from 1500pupils from 2 primary schools in Jinhua city by stratified cluster sampling method. Participants performed 3 different saccade tasks: 1 prosaccade and 2 antisaccades executed in a single-task and a dual-task situation. One of the antisaccade tasks was performed under the verbal secondary task condition to investigate verbal working memory.Saccades were registered by Eyelink Ⅱ Tracker. Errors and correct rate were analyzed. One-way ANOVA and repeated measures ANOVA were used to analyze the data. Results (1) Each group showed more response suppression errors on antisaccade task without load than on prosaccade task, ADHD-combined and ADHD-inattentive exhibited more errors than non-ADHD (P＜0.01) ,and errors was lower in ADHD-inattentive than in ADHD-combined ( ( 70.90 ± 15.78 ) % vs ( 84.04 ± 12.84 ) %, P ＜ 0.05 ). (2) The interactions between group and load were not significant in error rate on antisaccade tasks (P ＞ 0.05 ), but in correct rate (P ＜ 0. 05 ). The two subtypes of ADHD were affected more than non-ADHD, their correct rates tended to decrease under verbal working memory load. Conclusion An integrative view of deficits in inhibition and verbal working memory in two subtypes of ADHD is proposed, and ADHD-inattentive has a better response suppression than ADHD-combined.

BACKGROUND:It is necessary to carry out multiple operations to remove the scar in patients with large area of scar, and whether the scar tissue can be recycled has become the focus of the study. OBJECTIVE:To compare the tissue structure, biomechanical properties and biocompatibility of the acel ular dermal matrix of mature scar tissue and normal skin. METHODS:The acel ular dermal matrix was prepared from the human mature scar tissue and the normal skin around the scar. Subsequently, histological and scanning electron microscope observations were performed, and biomechanical properties were detected using universal tensile testing machine. Then, the acel ular dermal matrix from mature scar tissue and normal skin was co-cultured with fibroblasts for 10 days, respectively, and the cel growth curve was drawn. Additional y, the acel ular dermal matrix from mature scar tissue and normal skin was subcutaneously implanted into the dorsal tissue of Sprague-Dawley rats, respectively and histological observation was conducted at 4, 8 and 12 weeks after implantation. RESULTS AND CONCLUSION:There were many gaps but no cel ular components in the acel ular dermal matrix, in both two groups. Col agen fibers of the acel ular dermal matrix derived from mature scar were looser than that of the normal skin, and arranged slightly irregularly;the biomechanical properties of the acel ular dermal matrix derived from mature scar were similar to that of the normal skin, which exhibited appropriate flexibility and strength. There was no significant difference in the growth state of the two kinds of acel ular dermal matrix, and the growth curve was basical y consistent. After 4 weeks of implantation, more inflammatory cel s infiltration could be found in the mature scar group, and in contrast, only a few inflammatory cel s infiltration appeared in the normal skin group, These inflammatory reactions disappeared with time in both two groups. Besides, col agen fibers arranged in neat, and smal vessels grew into the implants in both two groups. In conclusion, the tissue structure, biomechanical properties and biocompatibility of the acel ular dermal matrix derived from scar tissue are almost consistent with those of the human normal skin.

Objective To study the relationship between the degree of intracranial and extracranial arterial stenosis and the plasma level of homocysteine ( Hcy ) in patients with ischemic cerebrovascular disease .Methods 96 patients with ischemic cerebrovascular disease were checked by DSA .The distribution of stenosis in the studied cerebral arteries was analyzed .According to the degree of stenosis ,all 96 patients were divided into some groups ,at the same time Hcy level was detected ,and the correlation between Hcy level and intracranial and extracranial artefial stenosis were analyzed .Results 35 of the 96 patients had normal findings in angiography ,and 61 cases had occlusive lesions of different degrees .The mean plasma Hcy concentration in patients with intracranial and extracranial arterial stenosis was 19.26 (14.79~26.13)μmol/L,which was significantly higher than 11.60(8.8~15.11)μmol/L in the patients with no arterial stenosis (P<0.05).There was a significant positive correiation between Hcy level and cerebral artery stenosis degree(r=4.126,P<0.05).Conclusion The plasma Hcy level was positively correlated with intracranial and extracranial arterial stenosis in patients with ischemic cerebrovascular disease ,incidence and nar-row-degree of cerebral artery stenosis are all along with the increasing of Hcy level .

Objective To study the effects of mild hypothemia therapy on coagulatic-fibrinolytic functions of the severe head injury patients.Methods 35 severe head injured patients from Jan 1998 to May 2000 were treated by mild hypothermia therapy and compared with past 30 patients.All patients's coagulate and fibrinolytic index was measured.Results All patients have coagulate- fibrinolytic disorders, mild hypothermia therapy can reduce consume of coagulate factors and inhibit secondary fibrinolytic function.Conclusion Mild hypothermia therapy is important to the severe head injury patients.

Objective: We have studied the role of lymphoproliferative tumors in the pathogenesis of autoimmune and the origin of the autoantibodies in paraneoplastic pemphigus (PNP) in recent years. A Castleman’s tumor from a patient was identified to produce autoantibody. To identify the relationship between the tumor and pathogenesis of the disease, we analyzed the rearrangement of immunoglobulin variable region gene and its hypermutation in B cells of Castleman’s tumor from a patient who was diagnosed of paraneoplastic pemphigus. Methods: The surface-markers of cultured tumor lymphocytes were assessed with immunochemistry staining. After total RNA of the tumor cells were isolated, the mRNA was reversely transcribed into cDNA. V H and V L genes were cloned and their sequences were analyzed. Results: Immunochemistry staining and flow cytometer analysis showed that the tumor cells were CD20, HLA-DR, smIgM, and smIgG positive. The cloned IgV H and IGHV3-9*01 germ-line gene are homologous and so are the Ig V L and the IGKV4-1*01 germ-line gene. More nucleotide changes in the V H or V L occurred in CDRs than those in FRs. Conclusion: In this reported case, a clone of specific B-lymphocyte in the Castleman’s tumor carrying functional rearranged immunoglobulin heavy and light chain genes was found to have experienced switch recombination and was possible to produce IgG autoantibody.

Objective To assess the influence of different methods of blood sampling on the results detected by instant glucose monitor.Methods We searched the following electronic databases CNKI and VIP(from 1995 to 2007).Five quasi-RCTs involving 305 participants were included,RevMan 4.2.10 was used for statistical analysis.Results There was significance in results between instant glucose monitor and auto-analyzer by finger massage and extrusion(WMD=-1.32,95%CI:-2.05 to-0.59,WMD-2.11,95%CI:-2.95 to-1.27,respectively),but no difference by natural outflow(WMD=-0.17,95%CI:-0.53 to 0.19).Conclusion There are influence on the blood glucose results by different methods of blood sampling,the natural blood outflow of fingers is the better way.