BACKGROUND: By varying the collected blood volume and storage temperature of the blood culture bottles prior to entry in an automated blood culture system, growth of organisms will be affected. METHODS: Blood culture bottles with a 20 mL blood volume per set were stored at 37degrees C (1st period) and room temperature (RT, 2nd period) upon arrival at the laboratory after working hours compared to baseline period (10 mL, RT). The time to detection (TTD) for all strains and the number of days until the final report after bottle entry were compared among the three periods. RESULTS: The median TTD for all strains was 13.5 h, 10.6 h, and 11.3 h in the baseline (N=268), 1st (N=454), and 2nd period (N=370), respectively (P<0.001). The final identification report was available within two days of bottle entry for 12.3%, 30.6% and 15.1% of bottles in the three different periods, respectively (P<0.001). CONCLUSION: Collecting an adequate blood volume is critical to reduce TTD. The preincubation of blood culture bottles at 37degrees C during the night shift might enable earlier final reports than storage at RT for samples with the same collected blood volume.
Blood Volume*

No abstract available.
Stroke Volume*

No abstract available.
Blood Volume

OBJECTIVEThe same person's pulmonary venous blood volume, left atrial volume and stroke volume were measured by lung CT scans and cardiac CT angiography (CTA). Then their relationships were analyzed in order to investigate the mechanism of breathing control.

METHODSAs we described before, full pulmonary vascular (-0.6mm) volume was accurately calculated by three-dimensional imaging technology from lung CT scan; left atrial volume and stroke volume of left ventricle were calculated from the CTA data. Then the relationships among them were analyzed for estimation of the lung-artery time.

RESULTSThe total volume of lung and pulmonary vascular blood was 3486 ± 783 (2156-4418) ml, and the pulmonary vascular blood volume was 141 ± 20 (105-163) ml. The estimated pulmonary venous volume was 71 ± 10 (52-81) ml. Left atrial volume at the end diastolic was 97 ± 39 (53-165) ml, Stroke volume of left ventricle was 86 ± 16 (60-106) ml. Pulmonary venous volume and the left atrial volume were double of stroke volume(1.7-2.4).

CONCLUSIONThe estimated lung-artery time was three heart beat.

No abstract available.
Doxorubicin* ; Incidence* ; Stroke Volume*

At the first step of studying about “the volume of cord blood” in Vietnamse, a total of 50 samples have been collected. We obtained some results: - The mean volume of cord blood was: 50.06 +/- 20,73ml. -The mean number of total mononuclear cells was 352.4 +/- 161.6 (x106). -Some cord blood colections had many total MNC as some larger cord blood harvests. So, it is our belief that total MNC numbers are more useful indicator of clinical utility than more sample volume.
Fetal Blood ; Blood Volume

Introduction: There is no single haemodynamic parameters either static central venous pressure (CVP) or dynamic stroke volume variation, inferior vena cava distensibility index (SVV,IVCd) that can be used precisely to assess fluid responsiveness. It must be performed concurrently with clinical assessment. Therefore, this study was conducted to determine the correlation between these 3 parameters. Methods: This was a cross sectional non-interventional study conducted in intensive care unit. Each patient who fulfilled the criteria will have their CVP, SVV and IVCd measured instantaneously. Analysis of correlation was done using bivariate (Pearson) correlation, while agreement between SVV and IVCd was assessed using Cohen’s Kappa analysis. Results: A total of 37 patients were enrolled in this study. 70.3% were males and 29.7% were females. Mean age was 59.7 ± 13.3. Mean APACHE score was 24.1 ± 6.1. IVCd had significant positive correlation with SVV (r = 0.391, p = 0.017). Agreement between IVCd and SVV was 0.329 (0.95 CI = 0.0174 – 0.6412; p = 0.033). There was non-significant negative correlation between IVCd with CVP and SVV with CVP with r = -0.155 (p=0.359) and r = -0.068 (p= 0.691) respectively. Conclusion: There is only fair correlation between IVCd and SVV in determining fluid responsiveness. However, CVP does not correlate to both SVV and IVCd. Neither one of them is a good method in assessing fluid responsiveness during standard care in our centre. Therefore, the usage of above methods needs to combine with clinical parameters to yield better result.
Stroke volume variation

No abstract available.
Stroke Volume* ; Stroke*

Bolld volume in 10 adult rabbits were measured by means of RISA dilution. The values were: eye plasma volume 0.0093 ml/gm eye, eye bolld volume 0.016 ml/gm eye and total blood volume of whole eye was 0.043 +/- 0.013(S.D.) ml. Total body plasma volume obtained simultaneously was 4.0% body weight and total body blood volume 6.9% body weight.
Adult ; Blood Volume* ; Body Weight ; Humans ; Plasma Volume ; Rabbits

BACKGREOUND: Several formulas for estimating allowable pre-transfusion blood loss were used to reduce unnecessary intraoperative blood replacement. The postoperative predicted hematocrit computed by formula was compared with the actual hematocrit and was tested which formula was more accurate in spine surgery. METHOD: Total blood volume was estimated in spine surgery of 34 patients. The target hematocrit (Hct) was suggested on 30% and the allowable blood loss was computed using the formula 1 and 2. For each patients, simultaneous measurement of blood loss and Hct was obtained at the end of operation. The postoperative predicted Hct by the formula 1 and 2 was calculated and compared with the actual Hct, and the difference between the formula 1 and 2 was evaluated. Allowable blood loss=Estimated blood volume (Initial Hct Target Hct)/Initial Hct ... Formula (1) Allowable blood loss=Estimated blood volume (Initial Hct Target Hct)/Average Hct ... Formula (2) RESULTS: 1) The preoperative Hct was 40.7 3.9%. The postoperative predicted Hct by the formula 1 and 2 were 34.3 4.6 and 34.9 4.3% respectively. The postoperative actual Hct was 30.1 4.6%.2) The difference between the predicted Hct by the formula 1 and the actual Hct was 4.2% (P<0.05). The difference between the predicted Hct by the formula 2 and the actual Hct was 4.8% (P<0.05). The difference between the predicted Hct by the formula 1 and 2 was 0.6% (P<0.05). It was thought that the predicted Hct by the formula 1 was more closer to the actual Hct. CONCLUSIONS: The predicted Hct by both formulas is underestimated when the results compare with the actual Hct. But the predicted Hct by the formula 1 provides a closer results to the actual Hct than the predicted Hct by the formula 2.
Blood Volume ; Hematocrit* ; Humans ; Spine