No abstract available.
Encephalitis, Viral*

Introduction: Dengue virus (DENV), the causative agent of dengue disease exists in sylvatic and endemic ecotypes. The cell morphological changes and viral morphogenesis of two dengue ecotypes were examined at the ultrastructural level to identify potential similarities and differences in the surrogate model of enzootic host. Materials and Methods: Vero cells were inoculated with virus at a multiplicity of infection (MOI) of 0.1. Cell cultures were harvested over a time course and processed for transmission electron microscopic imaging. Results: The filopodia protrusions on cell periphery preceded virus entry. Additionally, sylvatic DENV infection was found spreading slower than the endemic DENV. Morphogenesis of both dengue ecotypes was alike but at different level of efficiency in the permissive cells. Conclusions: This is the first ultrastructural study on sylvatic DENV and this comparative study revealed the similarities and differences of cellular responses and morphogenesis of two dengue ecotypes in vitro. The study revealed the weaker infectivity of sylvatic DENV in the surrogate model of enzootic host, which supposed to support better replication of enzootic DENV than endemic DENV.
; viral morphogenesis

Background: Hepatitis C Virus (HCV) is one of thecauses resulting in chronic hepatitis, cirrhosis and primary hepatocarcinoma. In Viet Nam, HCV-infected people are on the increase. The co-existence of HCV and G (HGV) in Viet Nam and their accurate genotyping needs to be clarified. Objective: To detect and molecularly genotype HCV and HGV from 4 serum isolates. Materials: This study consisted of 4 anti-HCV positive [HCV(+)] serum isolates. Method: To extract genomic RNA, perform RT-PCR, 5\u2019UTR fragment of 295 nucleotides for HCV, genomic RNAs from HCV(+) (confirmed by RT-PCR) as samples for RT-PCR for HGV to obtain 260 bp 5\u2019UTR. All of them were cloned and sequenced for analysis. Results: HCV products and HCV + HGV products obtained from samples, respectively; suggested that the co-existence of HCV and HGV could occur in a patient. Base on Gen Back and analysis, we showed that three nucleotide strains of HCV (HCV-H1VN, HCV-H2VN, HCV-H3VN) belongs to genotype 1a of the group 1a/1b, commonly found in East and Southeast Asia. HGV of Viet Nam [HGV-(Han9)VN strain] was identified to belong to type 2; one of five HGV types existing in the world. Conclusions: Three HCV isolates were genotype 1a; one HGV isolate belonged to genotype 2. Detection and genotyping of co-existence of HCV and HGV contributed the development of a multiplex-PCR/RT-PCR for screening blood-transmitted viral hepatitis.
Viral hepatitis ; genotype

In this research, 67 patients with acute viral hepatitis are divided in two groups: one group of 33 patients used Chµm TÝa infusion, the other of 34 patients didn't use it. We studied both their clinical and paraclinical changes and made the comparisons. The results are shown as follows: Good effect has been demonstrated in the group of moderately severe patients with typical acute viral hepatitis at dose of 90g of fresh leaves. Chµm tÝa has decreased more rapidly these symptoms: tiredness, poor appetite, dyspepsia, jaundice. These improvements are significant with p< 0.05. Chµm TÝa has normalized the total bilirubinemia, ASAT, ALAT, more rapidly in comparison with the control group and it has shortened significantly the treatment course (p<0.05).
Hepatitis ; Hepatitis, Viral, Human

No abstract available.
Child* ; Encephalitis, Viral* ; Humans

No abstract available.
Meningitis* ; Meningitis, Viral*

Hemorrhagic Fevers, Viral ; Adrenochrome

Research ; Hemorrhagic Fevers, Viral

At the Department of Communicabe Disease of Ha Noi – Dong Da Hospital 546 patients (52 dengue fever (DF) patients and 44 dengue hemorrhagic fever (DHF) patients were studied. IgM antibody was quantified by ELISA, blood count by 680 plus device. Results: a leucopenia (<4000) 24.2% in DF and 15.9% in DHF. Thrombocytopenia 51.2% in DF. In DF, 81.8% exerted an antibody response; in DHF there were a high rate of stimulating lympho cells (84.1%), there is 100% correlation between stimulating lympho cell and IgM antibody response
Dengue ; Hemorrhagic Septicemia, Viral ; Diseases

The diagnosis of viral hepatitis were based mainly on the determination of inflammation status and the necrosis of liver cells, the ruling out of non-viral causes of inflammation such as INH, acetaminophen, pneumococcus,…, and the examinations of epidemiology, clinics, biochemistry, immunology and micrology. The classification of the causes of viral hepatitis relies mainly on the serological, immunological and microbiological tests
Hepatitis, Viral, Human ; Hepatitis ; Inflammation