A study on the effects of Morinda citrifolia (MC) extract on the isolated blood vessels and water-electrolyte excretion by Lipschitz method. Results: MC in both concentrations of 1.25% and 2.5% had a vascular dilating effect of the rabbit ears without change vasoconstriction effect of adrenalin with concentration of 1/10 000. By oral administration, MC in doses of 0.3g, 0.6g and 0.9g/kg body weight increased the elimination of Na(+), K(+), Cl(-), but did not influence the elimination of Ca++ and urine amount at the rest time
Morinda ; Blood Vessels

Study on the extract of Morinda citrifolia (MC) fruit with the ratio of 5:1 (1g extract was prepared from 5g of dried MC fruit) in order to determine the effects of extract on radiation-induced immunosuppressive mice. The results showed that: oral administration of this extract with daily dose of 6 g/kg in 9 consecutive days (3 days before and 6 days during receiving gamma ray) has increased the relative weight of spleen and thymus, total leukocyte count, number of lymphocytes, natural killers and monocytes, rate of immune rosette forming cells, hemolytic halo, and the skin test with OA antigen, compared with the mice group that has been only received gamma ray without drug
Morinda ; Animal Experimentation ; Radiation

Background: Soc Son is mountainous, poorest district of Ha Noi, the rate of malnourished children was highest level (28,2% in 2003). Objectives: Description and assessment on the effectiveness of microcredit program for developing household economy and the impact of microcredit program on care for pregnant women, children and nutrition situation for children in 7 communes of Soc Son district in 3 years (2003-2006). Subjects and method: 167 poor households with malnourished child/children or pregnant women with low weight gain which were provided loans in 7 communes of Soc Son district; 169 children were born after 1/6/2001 and their mother in 167 households above. Method: cross-sectional descriptive method combine comparative analysis, using interview ballot, growth chart of National Institute of nutrition. Data processing by software EPI 6.0 and SPSS/PC 12.0. Results: The microcredit program had helped 95,0% households improve their economy remarkably, 54,5% of the households had improved income and food security. These improvements had influenced positively on practice of maternal and child care and malnutrition situation among children under 5 years old. The rate of pregnant women taking sufficient rest before birth delivery and having appropriate workload during pregnancy increased by 29,7%. Low-birth-weight newborns (<2,500 gram) had decreased by 11,5%. The rate of children with more than 3 complementary meals had increased by 8,5%. Malnutrition prevalence of children under 5 years old in 7 communes had decreased by 6,9%. Conclusion: The program has contributed to lower malnutrition prevalence of children under 5 years old in Soc Son district by 6,6%.
Child Nutrition Disorders/ diet therapy ; epidemiology ;

Background: many studies showed that obesity rate was significantly higher in women than in men and 60-80% of overweight/obesity caused by diet. Hanoi and Ho Chi Minh City were two of 10 provinces that had the highest rates of obesity. Objectives: to determine the actual rate of overweight/obesity and describe some related factors among Hanoians aged 50-59. Subjectives and Method: a cross sectional survey within a case-control study. The study was carried out in 14 wards under 7 districts of urban Hanoi. Body fat percentage was measured by machine Omron (Japan). Overweight/obesity evaluations were based on classification scales of WHO 1998 and IDI&WPRO 2000. Results: the mean rate of overweight/obesity was 23.1% (26.2% in women vs. 21.9% in men). The rate of women with waist to hip ratio >0.85 (39.83%) was higher than that in male (29.38%). Proportions of body fat percentage greater than 30% were 58.40% in female and 40.06% in male. Overweight/obesity was closely related with sedentary lifestyle, such as spending less time for exercise and sports, high food expenditures, etc. It was found that people with overweight or obese family members were 3 times at risk of overweight/obesity higher /than others. That rates of overweight/obese people with elevated level of biomarkers were composed of 48.99% with high blood cholesterol (>5.2mmol/l); 9.69% with blood glucose (\ufffd?.0mmol/l); especially 65.33% with high triglyceride (>1.7mmol/l); 7.22% with low HDL-C (<1.15 mmol/l); and 8.23% with high LDL-C (\ufffd?.00mmol/l). Conclusions: rate of overweight/obesity among Hanoians aged 50-59 was relatively high. Some related factors were described: waist to hip ratio, body fat percentage, sedentary lifestyle, such as spending less time for exercise and sports, high food expenditures, etc.
Overweight/epidemiology ; Obesity/epidemiology ; Risk Factors ; Middle Aged ;

Background: Human Heparansulphate interacting protein (hHip) has been shown to participate in biological processes of cells. Several studies indicated that hHip transcript is up regulated in several of cancer tissues including those of thyroid, colon, breast and prostate. Antibody against hHIP is necessary for methods to evaluate protein level of HIP in cancer tissues. Objectives:The aims of study was to induce anti hHIP antibody in rabbit and purify and conserve purified anti hHIP antibody. Subjects and method: The study included 9 adult and healthy rabbits with the weight 2 - 2.5kg. Immunization hHIP peptide-KLH in rabbit. Purify anti hHIP antibody using affinity chromatography. Results: The results shown synthesize hHIP peptide and conjugate it with carrier protein. Sensitive rabbit better meet with hHIP-KLH antibody. The Ig concentration obtained in sensitive rabbit was rather high and equal. Immunization hHIP-KLH successfully in rabbit. Obtainment valuable amount of anti hHIP antibody. Conclusion: Successfully induce and purify anti hHIP antibody from rabbit. Establish a standard protocol for polyclonal antibody against small peptide in rabbit.\r\n', u'\r\n', u'
Carrier Proteins/ administration & ; dosage ; chemistry ; Rabbits

Background: In traditional medicine, liquid extract VK2 has good effects in the treatment of osteoarticular pain. This remedy has its origin from \u201cTam ty thang\u201d of the Eastern traditional medicine. Objective: To evaluate the analgesic and acute anti-inflammatory effects of liquid extract VK2. Subjects and method: The trial was performed on white mice (18-22 g body weight) and albino rats (120 +/- 20 g body weight). These animals were healthy. The prescribed amount of VK2 doses were 27.5 g and 55 g per kg body weight of the mice, 19.25 g and 38.5 g per kg body weight of the rats. Results: The liquid extract of VK2 doses had analgesic effect on acetic acide and themal-induced pain in the mice. The liquid extract of VK2 doses had also acute anti-inflammatory effect through inhibiting carrageenine-induced edema in the hind paw. Conclusion: VK2 had analgesic and anti-inflammatory effects on experimental animals.
VK2 ; analgesia ; anti-inflammatory

Background: Pediatric closed abdominal trauma is a common disease in surgical practice, in which spleen is the most vulnerable organ. As adults, diagnosis of pediatric splenic trauma is not difficult but treatment attitude of splenic trauma in children having more important differences compared with in adults, tending to conservative treatment without surgery. Objectives: To assess the results of pediatric splenic trauma treatment in Hue Central Hospital from 2001 to 2005. Subjects and method: To study on 43 pediatric patients (23 male, 20 female), mean age 11.35\xb13.51 years, were diagnosed with splenic trauma due to closed abdominal trauma, treated in Hue Central Hospital from September, 2001 to September, 2006. Results: 72.1% hospitalized patients with maximum blood pressure in normal limitation. There was not a statistic significant association between hypotension and indicated surgery. The rate of patients with splenic trauma due to traffic accidents, living accidents and sport activities were 62.8%, 32.6% and 4.7%, respective. 2 patients with combined traumatic brain injury without surgery (4.65%), 3 patients with combined hand fracture (6.97%). 3 patients in conservative treatment group changed to surgery (8.33%). A total of 76.7% patients treated with successful conservation. Conclusion: More than 60% patients with splenic trauma caused by traffic accidents and having stable hemodynamic index during hospitalization. The difference between position of splenic trauma and rupture degree of spleen both in surgical treatment group and successful conservative treatment group is statistically significant.
Spleen/ injuries ; Child ;

Background: Hypertensive with impaired fasting glucose is the basic expression of metabolic syndrome. Objectives: To study the IR and the correlation between Fasting Plasma Insulin (FPI) and Fasting Plasma Glucose (FPG) concentration in hypertensive patients with Impaired Fasting Glucose (IFG). Subjects and method: The descriptive, cross-sectional study was carried out on 38 hypertensive patients with IFG (IFG group) and 30 hypertensive patients without IFG (control group). The data were collected and analyzed by SPSS 11.5 software. Results:The FPI and IR index in the IFG group were 14.6+/-5.2 micro mol/ml and 3. 94+/- l.40, respectively, higher than in the control group (9.6+/-4.2 micro mol/ml and 2.17+/-0.99/ respectively) with p<0.00l. There was a positive correlation between FPI and FPG systolic blood and diastolic blood pressure/ with correlation coefficients were 0.4/ 0.48 and 0.46/ respectively (p<0.00l). Conclusion: There was an increase of FPI and IR index in the hypertensive patients with IFG compared to those without IFG. \r\n', u'\r\n', u'
Insulin resistance ; hypertensive patients ; impaired fasting glucose.

Spores of Aspergillus sp. SU14 were treated repeatedly and sequentially with Co60 gamma-rays, ultraviolet irradiation, and N-methyl-N'-nitro-N-nitrosoguanidine. One selected mutant strain, Aspergillus sp. SU14-M15, produced cellulase in a yield 2.2-fold exceeding that of the wild type. Optimal conditions for the production of cellulase by the mutant fungal strain using solid-state fermentation were examined. The medium consisted of wheat-bran supplemented with 1% (w/w) urea or NH4Cl, 1% (w/w) rice starch, 2.5 mM MgCl2, and 0.05% (v/w) Tween 80. Optimal moisture content and initial pH was 50% (v/w) and 3.5, respectively, and optimal aeration area was 3/100 (inoculated wheat bran/container). The medium was inoculated with 25% 48 hr seeding culture and fermented at 35degrees C for 3 days. The resulting cellulase yield was 8.5-fold more than that of the wild type strain grown on the basal wheat bran medium.
Aspergillus ; Cellulase ; Dietary Fiber ; Fermentation ; Hydrogen-Ion Concentration ; Magnesium Chloride ; Methylnitronitrosoguanidine ; Polysorbates ; Seeds ; Spores ; Sprains and Strains ; Starch ; Triticum ; Urea

A fungal strain producing a high level of cellulase was selected from 320 fungal isolates and identified as Aspergillus sp. This strain was further improved for cellulase production by sequential treatments by two repeated rounds of gamma-irradiation of Co60, ultraviolet treatment and four repeated rounds of treatment with N-methyl-N'-nitro-N-nitrosoguanidine. The best mutant strain, Aspergillus sp. XTG-4, was selected after screening and the activities of carboxymethyl cellulase, filter paper cellulase and beta-glucosidase of the cellulase were improved by 2.03-, 3.20-, and 1.80-fold, respectively, when compared to the wild type strain. After being subcultured 19 times, the enzyme production of the mutant Aspergillus sp. XTG-4s was stable.
Aspergillus ; beta-Glucosidase ; Cellulase ; Mass Screening ; Methylnitronitrosoguanidine ; Mutagenesis ; Sprains and Strains