Several studies have indicated that retinal pigment epithelial(RPE) cells migrate from their normal location into the vitreous cavity where they then undergo proliferation and membrane formation in proliferative vitreoretinopathy(PVR). Little attention has been given to the role of vitreous on cellular proliferation. Our study is to determine the effect of vitreous on RPE cell proliferation and to examine the morphology of cultured RPE cells on vitreous explants. The vitreous from pigmented rabbit was extracted and added to the cultured media RPE cells proliferated rapidly along the margin of the vitreous as fibrocyte like cells and were less invasive into the virtrous gel. Liquified vitreous with media stimulated the proliferation of RPE cells, but vitreous alone showed the decrease of inoculated RPE cells.
Cell Proliferation ; Membranes ; Retinaldehyde* ; Vitreoretinopathy, Proliferative

We experienced a case of D3 proliferative vitreoretinopathy complicated with a shortened retina in a 31-year-old male which was managed using 360-degrees circumferential retinotomy technique and a case of D1 proliferative vitreoretinopathy complicated with a shortened retina in a 26-year-old female which was managed using 260-degrees circumferential retinotomy technique. We report these cases with a review of the literature
Adult ; Female ; Humans ; Male ; Retina* ; Vitreoretinopathy, Proliferative

Perfluorodecalin, which is one of the perfluorocarbon liquids, is not established safety in use of long-acting intraocular tamponade. Therefore, to determine its safety we injected it alone and combined with silicone oil into the vitreous of vitrectomized eyes. We evaluated the changes of the fundus, electroretinogram, histopathology as light and electron microgragh after lensectomy and vitrectomy in pigmented rabbits periodically. In rabbits replaced with perfluorodecalin alone, fundus showed mild proliferative vitreoretinopathy and micrographs showed the destruction of the inner and outer segments of the photoreceptors. In rabbits replaced with perfluorodecalin and silicone oil, fundus showed more severe proliferative vitreoretinopathy than perfluorodecalin alone and micrographs showed the destruction of the entire retina. In electroretinogram, the amplitude was decreased markedly. So, it is considered that perfluorodecalin was not tolerant in case of longacting intraocular tamponade and also perfluorodecalin combined with silicone oil developed severe proliferative vitreoretinopathy.
Rabbits ; Retina ; Silicone Oils* ; Vitrectomy ; Vitreoretinopathy, Proliferative

The authors performed experimental study to produce proliferative vitreoretinopathy by intravitreal injection of homologous fibroblasts in pigmented rabbits. Twenty four eyes of 12 rabbits were divided into control and experimental groups. In experimental group, the eyes were pretreated with expanding gas, perfluoropropane for the vitrectomy. In control group, no such procedure was preceded. In both groups, 5.0×10⁴, 1.0×10⁵ and 1.5×10⁵ cells of homologous fibroblasts were injected into the vitreous cavity. All the eyes were observed regularly with indirect ophthalmoscope followed by fundus photography. Those eyes were enucleated in 1, 2, 4 weeks for the light and electron microscopic studies. The development and severity of the proliferative vitreoretinopathy were correlated with the numbers of the injected fibroblast. This was more pronounced in the experimental group.
Fibroblasts ; Intravitreal Injections ; Ophthalmoscopes ; Photography ; Rabbits ; Vitrectomy ; Vitreoretinopathy, Proliferative*

We reviewed charts of patients on the clinical characteristics and factors which affect the prognosis of surgical results in the 77 patients(77 eyes) who were diagnosed with rhegmatogenous retinal detachment and operated on and followed for at least 3 months postoperatively at Korea University Guro hospital. We evaluated the characteristics of rhegmatogenous retinal detachment in Korea by comparing with other reports on retinal detachment as well. The factors associated with anatomic success were the duration of retinal detachment and the prescence of proliferative vitreoretinopathy. High success rate was achieved when the duration was less than 1 month and there was no proliferative vitreoretinopathy. The factors associated with functional success included the duration of retinal detachment, proliferative vitreoretinopathy, preoperative refractive error and the degree of retinal detachment(P<0.05).
Humans ; Korea ; Prognosis ; Refractive Errors ; Retinal Detachment* ; Retinaldehyde* ; Vitreoretinopathy, Proliferative

We reviewed 71 cases of silicone oil injection for retinal detachment surgery with advanced proliferative vitreoretinopathy following failed vitrectomy between November 1982 and March 1986 in order to identify the anatomic and functional success rate and its complications. The causes of vitrectomy were as follows: advanced proliferative vitreoretinopathy after retinal detachment surgery and retinal vascular diseases(34 eyes), giant dialysis(10 eyes), severe perforating injuries and intraocular foreign bodies(17 eyes), and traction retinal detachments associated with proliferative diabetic retinopathy(10 eyes). In 71 cases of silicone oil injection, anatomic success rate was 34.8%(23/66) and functional success rate was 16.9%(16/71) and its complications were lens opacities(28.1%, 9/32) in phakia and corneal opacities(20.5%. 8/39) and increased intraocular pressure(17.9%, 7/39) in aphakia.
Aphakia ; Retinal Detachment* ; Retinaldehyde* ; Silicone Oils* ; Traction ; Vitrectomy* ; Vitreoretinopathy, Proliferative*

Perfluorocarbon liquids have been shown to be a useful intraoperative adjunct in managing complicated retinal detachment. To prove the effectiveness of intraoperative use of perfluorocarbon liquid and the improvement of surgical and functional outcomes, we retrospcetively evaluated the clinical results of the patients[13 eyes]who had retinal detachment complicated with proliferative vitreoretinopathy [over PVR Grade C4, C4 :7 eyes, C5 :6 eyes]. We performed standard 3-port pars plana vitrectomy in all eyes included in this study.Perfluorodecalin[DK-line]was used as a shortterm vitreous substitute intraoperatively and removed before the end of the surgery. The mean follow-up period was 15 months.Anatomical success rate was 86%[6 eyes]in Grade C4, 67%[4 eyes]in Grade C5, Visual acuity was over 5/200 in one eye[8%]and there was improvement in 5 eyes[40%] although it was below 5/200. Consequently, we could easily manage the retinal detachment with PVR with help of intraoperative perfluorocarbon liquid and could improve the postoperative surgical and visual outcomes.
Follow-Up Studies ; Retinal Detachment ; Visual Acuity ; Vitrectomy ; Vitreoretinopathy, Proliferative*

In the article, the development of medical treatment for eye injuries in the mainland of China was reviewed. According to the data provided in Eye Injury Vitrectomy Study (EIVS), 27% of 72 eyes with no light perception (NLP) gained recovery in term of antomy and visual function. Vitrectomy initiated at more than 4 weeks after open eye injury is an independent risk factor for developing PVR. Prognosis of anatomy and visual function of the injured eye with PVR is markedly worse than that without PVR. Serious injuries of ciliary body, choroid and retina are three key parts of the eye with NLP. The concept that the treatment of the eye injury gradually focus on the whole globe is embodied. The data from 13575 in patients with traumatic eyes in 14 hospitals revealed that the rate of immediate enucleation was remarkable reduced with comparison of 20 years ago.
Eye Injuries ; therapy ; Humans ; Vitrectomy ; Vitreoretinopathy, Proliferative ; therapy

In vitro PVR(Proliferative vitreoretinopathy) models allow identification of factor which may inhibit porliferation and contraction. In this study we evaluated the contraction of collagen matrix by choroidal fibroblast and the inhibition of contraction by antipoliferative drug. Each antiproliferative drug showed inhibition of collagen matrix contraction : Colchicine (0.1microgram/ml), Cytochalacin (0.05microgram/ml), Puromycin(10microgram/ml). Transmission electron microgram of collagen matrices containing colchicine, cytochalacin or puromycin showed no collagen fiber surrounding choroid fibroblast and showed cell destruction. Scanning electron microgram of collagen matrices containing colchicine, cytochalacin and puromycin showed that collagen fibers were well preserved without distortion. Colchicine, cytochalacin and puromycin are effective inhibitor of cell mediated contraction in additon to it`s potent antiproliferative effect wherease Interfereon has no anticontractile effect. The current study present a model to investigate the effect of antiproliferative drug on fibroblast mediated collagen matrix contraction.
Choroid ; Colchicine ; Collagen* ; Fibroblasts ; Interferons ; Puromycin ; Vitreoretinopathy, Proliferative

Scleral buckling used alone to treat retinal detachment with proliferative vitreoretinopathy(PVR) of grade C resulted in an overall anatomic reattachment rate of 86%(39/45); 96%(27/28) in stage C1, 83%(10/12) in stage C2, and 40%(2/5) in stage C3. The success rate declined with increasing severity of PVR. Stage C3 eyes showed a significantly lower rate of retinal reattachment than stage C1 and C2 eyes(p<.05). We currently recommend scleral buckling alone as the surgery of choice for stage C1 and C2 PVR; we reserve vitrectomy with preretinal membrane removal for the more advanced cases.
Membranes ; Retinal Detachment ; Retinaldehyde ; Scleral Buckling* ; Vitrectomy ; Vitreoretinopathy, Proliferative*