Objective　To observe the presence of synergistic efficacy between adenovirus mediated bcl-Xs (Adv-bcl-Xs) gene therapy and chemotherapy on ovarian cancer cell. Methods　NuTu-19 cells were infected by different titers of Adv-bcl-Xs and were treated with topotecan at the same time. Cell proliferation was measured 3 days later by MTT. Graphical representations of the statistical analyses recorded their interaction in tumor cells. Results　The statistical results and graphical representations of the statistical modeling showed that the synergistic antiproliferative activity was present (P＜0.01). Conclusion　There were synergistic efficacies between Adv-bcl-Xs gene therapy and Topotecan on ovarian cancer cell.