Abstracts Traditional drug Lider-5 traditional medicine, rheumatism and arthritis disease which has been used experimental animals we created arthritis disease antioxidant system of the Lider-5 studied the impact of the loss. As a result of the research that you created via a test animal collagen II arthritis pathological articular tissues and blood plasma contained malondialidyegid In addition to reducing the antioxidant Glutathione is provided a system of the body, including supyeroksidismutaza enzymes to increase the amount of influence. As you can see in the traditional drug Lider-5 disorders antioxidant system, loss prevention, and treatment is effective, the effects of its ingredients contained Tinospora sinensis, Gentiana decumbens, such as antioxidant effect might be associated with key plant attendant substances.

Introduction. Monos Group, Drug Research Institute is starting to investigate of Ellipin preparationfrom the mid-1990s, Ellipin has anti cancer activity in liver and several studies were investigated withscientists from Japan and China. Especially Hayashi K., Khurelbaatar L and Ambaga M were determinedanti-cancer action of the preparation and they were explained of mechanism of action, which apoptosisis seduced by influence of unsaturated fatty acids in tumor cells. However, changes of fatty acidscomposition at production stage were did not study yet. Therefore, we studied that composition of fattyacids in different term of production stage and compared of Ellipin dense substance.Materials and Methods. Samples of study were collected from production stage of “Ellipin” series130304, which was tacked in 48th hour, 120th hour of production. Each sample was dried at freezedryer “Labconco freezone12L” in Drug Research Institute. Total lipids of sample were extracted withchloroform: methanol (2: 1 v/v) according to Folch et al. Fatty acid methyl esters were analyzed usingAgilent Packard Gas Chromatograph (GC) (Model HP-6890 Agilent Packard) with mass-spectrumdetector (Model HP MSD 5973N) of Buryat State University, in Ulan-Ude.Results. Ellipin preparation is derived from bovine liver, and which is based on homogenization of bovineliver for isotonic. In this process, unsaturated fatty acids were extracted in organic solution. We studiedchanges which saturated and unsaturated fatty acids of bovine liver in process of homogenization andconsist of each fatty acid contents of end product. Results have shown that unsaturated fatty acidswere decreased by 0.4-44% till 120th hour of homogenization process. While, there were decreasedby 4-12% in the end product, although, ω-6 fatty acids were increased by 13.1-38.4%. Moreover, 25saturated fatty acids and 12 unsaturated fatty acids were detected in the Ellipin dense substance (endproduct). Hence, 67.5% of total fatty acid was saturated fatty acids, 32.5% was unsaturated fatty acidsin the Ellipin dense substance. Resent results and results of previous studies indicated that Ellipindense substance may contains saturated fatty acids on in average 50.34%, unsaturated fatty acids onin average 49,32%, respectively.Conclusion. Proportion of saturated and unsaturated fatty acids in Ellipin production was about 2:1.Saturated fatty acids and unsaturated fatty acids were found 25 and 12, respectively. Saturated fattyacids were gradually decreased and unsaturated fatty acids were slowly increased in production period,which from 48th hour of production-conveyer till end product. Moreover, content of ω-3-6-9 fatty acidswas consist 83,9-87,5% of total unsaturated fatty acid.

Fracture healing starts with a hematoma, continues with bone formation, and ends with bone remodeling. We decided to investigate the effects of Jonsh-5 composition on the serum level of TGF-β1 during fracture healing. A Wistar rats were utilized. K- wire was inserted into the femur in a retrograde fashion, and a mid-diaphyseal closed transverse fracture was created using method of Bonnarens & Einhorn (1984). All rats were randomly in three groups. Group 1 (Control group, n=26) was given normal food without any medicine. Group 2,3 ( each group n=26) were administered 0.625 mg calcium supplement and 1.3 g Jonsh-5 composition with normal food. Serum analysis, histological evaluations were performed at days 1, 7, 14 and 28 and after femur fracture according to the criteria proposed by Huo et al. Serum analysis , there was significant difference treatment effect between Group 3 (Jonsh-5 composition) and Groups 1,2 at days 3,7,14 and 28 days after fracture. The histological results demonstrated a significant stimulation of the fracture healing due to group 3 compared to group 1,2 at days 14 and 28 .Under studied conditions this investigation shows that administration of Jonsh-5 accelerate fracture healing process in rat femur

Introduction. Garidi-5, a traditional medicine composed of 5 herbs including Terminalia chebula Retz., Aconitum Kusnezoffii Reichb., Acorus calamus L., Saussurea lappa L., and musk of Moschus moschiferus, has been used in traditional Mongolian medicine as an analgesic and antibacterial medicine. The present work was undertaken to evaluate the traditional drug Garidi-5 for its analgesic and anti-inflammatory activity.Materials and Methods. The method of Winter et al. was used to study acute inflammation. Rats in groups of five each were treated with vehicle, Garidi-5 (20, 80 and 200 mg/kg, p.o.) and Indometacin (10 mg/kg) one hour prior to Carrageenan injection. 0.1 ml of 1% Carrageenan was injected into the subplantar tissue of left hind paw of each rat. Swelling of carrageenan injected foot was measured at 0, 0.5, 2, 4 h using Plethysmometer (UGO Basile, Italy)). The right hind paw was injected with 0.1 ml of vehicle.Results. The Garidi-5 (20, 80 and 200 mg/kg) significantly (P<0.01) inhibited carrageenan induced rat paw edema as compared to control group. Maximum inhibition of paw edema was observed with Garidi- 5 (80 and 200 mg/kg) at 4 h when compared to the control group (Tab.1). In assay data, the TNF-α, IL-1β and IL-6 secretion in serum were highly elevated by carrageenan induction but administration of Garidi-5 signifi cantly reduced serum secretion of inflammatory mediatorsas compared to vehicle group (Tab. 2).Conclusion. In conclusion, Traditional drug Garidi-5 have anti-in flammatory properties. The potential efficacy of Garidi-5 to treat inflammation is based in a part on the hy pothesis that it will suppress the proinflammatory cytok ines resulting in less oedema.

Introduction. Garidi-5, a traditional medicine composed of 5 herbs including Terminalia chebula Retz. Aconitum Kusnezoffii Reichb., Acorus calamus L., Saussurea lappa L., and musk of Moschus moschiferus, has been used in traditional Mongolian medicine as an analgesic and antibacterial medicine. The present work was undertaken to evaluate the traditional drug Garidi-5 for its analgesic and anti-inflammatory activity.Materials and Methods. The method of Winter et al. was used to study acute inflammation. Rats in groups of five each were treated with vehicle, Garidi-5 (20, 80 and 200 mg/kg, p.o.) and Indometacin (10 mg/kg) one hour prior to Carrageenan injection. 0.1 ml of 1% Carrageenan was injected into the subplantar tissue of left hind paw of each rat. Swelling of carrageenan injected foot was measured at 0, 0.5, 2, 4 h using Plethysmometer (UGO Basile, Italy)). The right hind pawwas injected with 0.1 ml of vehicle.ResultsThe Garidi-5 (20, 80 and 200 mg/kg) significantly (P<0.01) inhibited carrageenan induced rat paw edema as compared to control group. Maximum inhibition of paw edema was observed with Garidi-5 (80 and 200 mg/kg) at 4 h when compared to the control group (Tab.1). In assay data, the TNF-α, IL-1β and IL-6 secretion in serum were highly elevated by carrageenan induction but administration of Garidi-5 signifi cantly reduced serum secretion of inflammatory mediators as compared to vehicle group (Tab. 2). ConclusionIn conclusion, Traditional drug Garidi-5 have anti-in flammatory properties. The potential efficacy of Garidi-5 to treat inflammation is based in a part on the hy pothesis that it will suppress the proinflammatory cytok ines resulting in less oedema.

Aim of the study Aim of the study: The aim of our work intends to define of peripheral analgesic effect of the traditional drug Garidi 5 in experimental animal models. Objectives of the study: 1. To determinate the LD50 of traditional drug Garidi-5 2. To study of analgesic effect of Garidi-5 on experimental animal models Material and Methods: Mongolian traditional drug Garidi 5 was produced from the traditional drug manufacture of TMSTPCorporation of Mongolia. - The LD50 of Garidi-5 determined by Chan Chi (2005) - Analgesic effect was assessed by glacial acetic acid-induced writhing test (for peripheral action). All the animals used in the study were taken care of under ethical consideration, with approval from Ethical Committee Corporation and Minister of Health of Mongolia. Analgesia by peripheral action: The peripheral analgesic effect was tested by glacial acetic acid–induced writhing test in Albinomice (Wilkin et al,1961). Healthy mice of either sex weighing 20-30 g were fasted overnight and divided into three groups with six animals in each group. One hour after administration of drugs, induction of writhing was done in mice by giving intra peritoneal injection of acetic acid at a dose of 10 ml/kg body weight. The number of writhing responses were counted and recorded for 20 min. Traditional drug of Garidi 5 (20 mg/kg , 80 mg/kg, 200 mg/kg) was used as the test drug. Aspirin was taken as standard drug at a dose of 100 mg/kg p.o. (Ghosh, 2008) Results: Traditional drug Garidi-5 is LD50=2,28±0,15, it’s means that drug has low toxicity. Aqueous extract of Garidi 5 had significant peripheral analgesic effect as compared to control (p<0.05).

Background and Aim: The traditional herbal formulation of “Sendeng- 4”have been used to treat ulcers. The wound healing activity of traditional herbal formulations (Sendeng-4) containing Acacia catechu Willd (FamilyFabaceae), Terminalia chebula Retz, Terminalia bellarice (Family Combretaceae), Gardenia jasminoides (family Rubiaceae). We were prepared lotion of “Sendeng-4” was evaluated for its wound healing potential of incision wound models in mice’s. Material and Methods: Healthy inbred white mice’s were weighing 20-22 used for the study. The animals were randomly divided into two groups(n= 10) in all the models. The mice’swere anesthetized ketamine (12mg kg–1bodyweight) of the experimental wounds. We used comparable to that of lotion of thestandard drug and standard solution used for comparison in this present investigation. The results were also comparable of wound contracting ability, epithelization period, regeneration of tissues at the wound area. On Day 14-16th lotion of “Sendeng-4” treated animals exhibited 100% reductionin the wound area when compared to controls. The increased rate of woundcontraction, skin breaking strength and of “Sendeng-4” in wound healing. Conclution:The lotion of traditional herbal formulation of “Sendeng-4” have facilitateswound healing in the experimental animal model.

Traditional drug Jonlon-5 have been widely used in the clinical practice for recent years in traditional medicine. The study was conducted on the experimental animal wistar rats by the carrageenan induced mice paw edema (Winter et al. 1962, Vogel, 2002) methods. We performed chemical and pharmacological investigation was based and implemented scientific research laboratory of Institute of Traditional Medicine and Technology. The Jonlon-5 significantly (P<0.05) inhibited carrageenan induced rat paw edema as compared to control group. TNF-α secretion was magnified by carrageenan injec¬tion (387.2 pg/ml). TNF-α secretion was reduced, com¬parable to carrageenan group, as a respond to extract administration. Administration of Jonlon-5 significantly reduced inflammatory mediator secretion TNF-α by 20.8%, IL-1 β – 41.7%, to be close to inhibition level of Indo¬methacin administration. Jonlon-5 significantly decreased (P<0.05) of serum malondialdehyde (MDA) 16.5% in animal model.

Objectives: To study of the effects on traditional drug Sendeng 4 for papain-induced osteoarthritis in rats. Methods: The rats were divided into three equal groups in number and were sacrificed under high-dose ether anesthesia after one, two, and four weeks of the last papain injection, respectively. Osteoarthritis was experimented in Wistar rats. Total 21 rats were injecting an admixture of 4% papain and its activator 0.03 M cycteine into the right knee joints on the first, fourth, and seventh days. The same volume of sterile saline solution was injected into the left knees as controls. Result. Pre sacrifice, measured with the artritis scoring evalution and X ray analysis, after cartilage from femorel condyles and tibial plateaus was macroscopically and histophatologically evaluated. The artritis scoring evalution were significantly lower at Sendeng 4 groups (2.2±0.16) compared to the control (3.7±0.25) groups. (p

Jonsh-5 is composed of 5 components including calcite, Cardius crispus, Forsythia suspense, Terminalia Chebula, and Inula helenium. Aim of this study is to examine the effect of traditional drug Jonsh-5 on experimental femur fracture in rats. Jonsh-5 was given to rats for 28 days. Calcium D3-Nycomed (2.5 g/kg) was used for comparison. 2, 14, and 28 days after fracture, serum levels of alkaline phosphatase were determined and fracture healing processes were evaluated by X-ray. Serum level of alkaline phosphatase was 197 u/l before fracture. After 7 days of fracture, alkaline phosphatase level was significantly increased by Jonsh-5 (728 u/l) and there were no statistical differences observed in rats with no treatment (control) and Calcium D3-Nycomed-treated animals. Similar patterns were observed 14 and 21 days after fracture. X-ray images showed that healing process of the bone was significantly increased by Jonsh-5 compare to control and Calcium D3-Nycomed treatment. Jonsh-5 may promote bone healing in rat model of fracture.