1.Clinical investigation on the correlation between lower urinary tract infection and cystitis glandularis.
Zhiqiang, CHEN ; Zhangqun, YE ; Wei, ZENG
Journal of Huazhong University of Science and Technology (Medical Sciences) 2004;24(3):303-4
In order to study the association between lower urinary tract infection and cystitis glandularis (CG), 120 cases of CG were diagnosed by cystoscopic biopsy in the suspicious foci of the bladder. Among them, 72 cases were subjected to bacterial counting culture of urine and microscopic examination of urinary sediment, and 60 cases to fluorescence quantitative polymerase chain reaction (FQ-PCR) assay to detect HPV, CMV and HSV DNA in urine samples. In the 72 cases of CG, the positive rate of bacterial counting culture of urine was 15.3% (11/72), and gray zone rate was 18.1% (13/72). 31.9% (23/72) patients were positive in bacterioscopy of urinary sediment. There was statistically significant difference as compared with the control group (P<0.01). Only 4 of 60 urine samples were positive by FQ-PCR in detection of the three viruses mentioned above with the positive rate being 6.67%. Compared with the control group, there was no significant difference (P>0.05). It was concluded that the genesis of CG was closely correlated with the chronic lower urinary tract infection, especially caused by Esch coli.
Cystitis/etiology
;
Cystitis/microbiology
;
Cystitis/*pathology
;
*Escherichia coli Infections
;
Metaplasia
;
Urinary Bladder/microbiology
;
Urinary Bladder/*pathology
;
Urinary Tract Infections/complications
;
Urinary Tract Infections/*microbiology
;
Urinary Tract Infections/pathology
2.Related factors of urinary tract infections in inpatients based on real world data.
Chun Hong BIAN ; Yue PAN ; Ya Nan TAN ; Li Min ZHANG ; Rong Qi WANG ; Guo Jun ZHANG
Chinese Journal of Preventive Medicine 2022;56(11):1636-1641
To analyze the risk factors for urinary tract infection (UTI) among inpatients. The case data of 1 875 inpatients receiving urinary bacterial culture in Beijing Haidian Hospital from October 2019 to May 2021 were analyzed retrospectively. According to the etiological diagnostic criteria of UTI, they were divided into infection group and non-infection group. The species and distribution of pathogens in the infection group were analyzed, and the case data and laboratory indexes were subjected to univariate analysis. The variables with statistical significance were selected for binary logistic regression to analyze the risk factors of urinary tract infection and establish a prediction model. The receiver operating characteristic (ROC) curve was drawn for each parameter included in the model, and the area under the curve (AUC) was calculated. The diagnostic and predictive efficacy of each parameter alone and their combination for UTI were evaluated. So, a total of 1 162 patients with non-infection group and 713 patients with UTI were detected. Among the cultured pathogens, the constituent ratio of Gram-negative bacteria, Gram-positive bacteria and fungi was 57.2%(408/713), 35.9%(256/713) and 6.9%(49/713) respectively. Multivariate analysis showed that, Age, duration of urinary catheterization>7 d, stroke and orthopedic surgery were the risk factors of UTI among inpatients. The use of antibiotics is a protective factor for urinary tract infections. The prediction model of UTI was established by the risk factors, age, duration of urinary catheterization>7 d, stroke, orthopedic surgery, urinary leukocyte esterase, urinary nitrite and Coefficient of variability of red blood cell volume distribution width (RDW-CV). The AUC of the combination of the eight parameters in diagnosing and predicting UTI was 0.835 (95%CI: 0.816-0.855), with the sensitivity of 70.7% and the specificity of 82.8%. In conclusion,the combination of the eight parameters can better assist in the diagnosis and prediction of UTI, and provide an experimental basis for clinicians to judge UTI.
Humans
;
Retrospective Studies
;
Inpatients
;
Urinary Tract Infections/microbiology*
;
Urinalysis
;
Stroke
4.Etiological study on cystitis glandularis caused by bacterial infection.
Xiaogang, LIU ; Zhiqiang, CHEN ; Zhangqun, YE
Journal of Huazhong University of Science and Technology (Medical Sciences) 2007;27(6):678-80
To study the relationship between bacterial infection and the etiology of cystitis glandularis, 36 female Wistar rats were divided into 3 groups. No intervention was given to the rats in the blank group. NS was infused into the bladder of the rats of the control group, and solution containing E. coli was injected into the bladder of experimental group. Three months later, tissue samples of bladder were collected and observed visually and under light microscope. The results showed that tissues of the blank group were normal; one sample in the control group showed Brunn's nests and cystitis cystica, and 10 in the experimental group had the change of cystitis glandularis. Compared to the blank and control group, samples in the experimental group showed significant change (P<0.05). There were no significant difference between blank group and control group (P>0.05). It is concluded that bladder instillation of E. coli can induce cystitis glandularis, which confirms that infection is the cause of cystitis glandularis.
Cystitis/etiology
;
Cystitis/*microbiology
;
Cystitis/*pathology
;
Escherichia coli Infections/*pathology
;
Rats, Wistar
;
Urinary Bladder/microbiology
;
Urinary Bladder/*pathology
;
Urinary Tract Infections/complications
;
Urinary Tract Infections/*microbiology
;
Urinary Tract Infections/pathology
7.Clinical investigation on the correlation between lower urinary tract infection and cystitis glandularis.
Zhiqiang CHEN ; Zhangqun YE ; Wei ZENG
Journal of Huazhong University of Science and Technology (Medical Sciences) 2004;24(3):303-304
In order to study the association between lower urinary tract infection and cystitis glandularis (CG), 120 cases of CG were diagnosed by cystoscopic biopsy in the suspicious foci of the bladder. Among them, 72 cases were subjected to bacterial counting culture of urine and microscopic examination of urinary sediment, and 60 cases to fluorescence quantitative polymerase chain reaction (FQ-PCR) assay to detect HPV, CMV and HSV DNA in urine samples. In the 72 cases of CG, the positive rate of bacterial counting culture of urine was 15.3% (11/72), and gray zone rate was 18.1% (13/72). 31.9% (23/72) patients were positive in bacterioscopy of urinary sediment. There was statistically significant difference as compared with the control group (P<0.01). Only 4 of 60 urine samples were positive by FQ-PCR in detection of the three viruses mentioned above with the positive rate being 6.67%. Compared with the control group, there was no significant difference (P>0.05). It was concluded that the genesis of CG was closely correlated with the chronic lower urinary tract infection, especially caused by Esch coli.
Adult
;
Cystitis
;
etiology
;
microbiology
;
pathology
;
Escherichia coli Infections
;
Female
;
Humans
;
Male
;
Metaplasia
;
Middle Aged
;
Urinary Bladder
;
microbiology
;
pathology
;
Urinary Tract Infections
;
complications
;
microbiology
;
pathology
8.Prevalence, associated risk factors and antibiotic resistance pattern of bacterial uropathogens among pregnant women in a tertiary care hospital of Bangladesh
Sohana Al Sanjee ; Masudur Rahman ; Md. Ekramul Karim ; Umme Salma Sigma
Malaysian Journal of Microbiology 2020;16(4):294-301
Aims:
The study was designed to determine the prevalence of urinary tract infection (UTI) in pregnant women
depending on their various clinical and socio-demographic factors, and to assess the antibiotic susceptibility pattern of
the responsible uropathogens in a tertiary care hospital of Dhaka, Bangladesh.
Methodology and results:
A total of 100 midstream urine samples were collected from pregnant women and different
clinical and socio-demographic variables viz. age, gestational weeks, living conditions, and level of education associated
with UTI were determined. Bacterial isolation was carried out using blood and MacConkey agar and identified according
to their phenotypic characteristics. Antibiogram profiling of the isolates was done by disc diffusion method. From 48% of
positive UTI samples, the highest bacteriuria was recorded within the age group of 26-30 years (n=19; 59.38%) and in
both, 1st and 3rd trimester period (50%). There was no significant association between the studied risk factors and
bacteriuria, except for the age of the pregnant women. Most predominantly isolated bacteria was Escherichia coli (n=39;
81.25%), followed by Klebsiella pneumoniae (n=9; 18.75%). In E. coli, the highest resistance was recorded against
ceftriaxone (87.18%), followed by cephalexin (84.61%) and ceftazidime (79.49%); whereas K. pneumoniae showed
100% resistance to ceftriaxone and cephalexin. Netilmicin was found as the only effective antibiotic against E. coli
showing 100% sensitivity. For K. pneumoniae, azithromycin, imipenem, chloramphenicol, gentamicin, ciprofloxacin,
amikacin and nitrofurantoin were found as the most efficacious drugs.
Conclusion, significance and impact of study
As the emergence of drug resistance is ever increasing, the study
necessitates the continuous surveillance of antibiotic susceptibility of uropathogens to ensure safety and better
treatment to the mother and fetus.
Urinary Tract Infections--microbiology
;
Drug Resistance, Microbial
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Pregnant Women
;
Bangladesh (Daka)
9.Gene detection of Neisseria spp. isolated from male patients with genitourinary tract infections.
He WANG ; Zhen-hua LUO ; Xu YI ; Xiao-ling SHE ; Dan-ni WANG ; Yan WANG ; Chang-yun YE
National Journal of Andrology 2012;18(1):39-43
OBJECTIVETo detect the genes of Neisseria spp. isolated from patients with male genitourinary tract infections, and to study the pathogenicity of non-gonococcal strains of Neisseria and the laboratory diagnosis for the infections caused by Neisseria spp.
METHODSUsing polymerase chain reaction and nucleotide sequencing, we amplified and sequenced 4 genes of Neisseria spp. isolated from patients with male genitourinary tract infections, including 16S rRNA, orfl, cppB and nspA.
RESULTSFourteen Neisseria strains were identified through analysis of the 16S rRNA gene, including 3 N. mucosa strains, 3 N. cinerea strains, 2 N. gonorrhoea strains, 2 N. sicca strains, 2 N. subflava strains, 1 N. lactamica strain, and 1 N. polysaccharea strain. Among them, 9 showed positive results in gonococcal fluorescence-labeled multiplex-PCR detection, 1 in cppB gene reaction, 5 in orfl gene reaction, and 3 in nspA gene reaction. The consistency rate was 85.7% between the above results from our gene detection and those from the routine bacteriological methods.
CONCLUSIONThe cppB gene is absent in the non-gonococcal strains of Neisseria spp. that can cause male genitourinary tract infection. Most of the strains not only lack virulence-associated orfl and nspA genes, but also show positive results in gonococcal fluorescence-labeled multiplex-PCR detection, which is one of the important reasons for the misdiagnosis and missed diagnosis of gonorrhea infection. The combination of routine bacteriological methods and gene detection in laboratory examinations may help improve the accuracy rates of Neisseria species identification and clinical diagnosis of the infections caused by Neisseria spp.
Genes, Bacterial ; Gonorrhea ; diagnosis ; microbiology ; Humans ; Male ; Neisseria gonorrhoeae ; classification ; genetics ; Polymerase Chain Reaction ; Urinary Tract Infections ; microbiology
10.Progress in TcpC research.
Journal of Zhejiang University. Medical sciences 2013;42(5):481-485
TcpC is a homolog of the Toll/interleukin-1 receptor (TIR) domain and is secreted by uropathogenic E. coli strain CFT073. TcpC can bind to MyD88, hereby exerting inhibitory effects on macrophages. TcpC represents an important virulence factor that promotes bacterial survival and pathogenicity. TcpC plays a critical role in urinary tract infection, particularly in the pathogenesis of pyelonephritis. In this review,the progress and prospects in TcpC research are discussed.
Animals
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Escherichia coli
;
pathogenicity
;
Escherichia coli Proteins
;
physiology
;
Humans
;
Mice
;
Pyelonephritis
;
microbiology
;
Urinary Tract Infections
;
microbiology
;
Virulence Factors
;
physiology