BackgroundThese study made us to investigate the drug row material of Mongolian traditional medicine such us Tribulus terrestris, Malva neclecta Wall and Eriocheir sinensis compounded preparation named “Malbus”. Investigated us preparations steel using for treatment of kidney and urinetract deseases, it is one of effective in Mongolian traditional drug preparations.Material and MethodsThe experimental protocol was approved by the Ethics Review Committee at the Ministry Health of Mongolia. In research we used 20 healthy mice and 60 Wistar rats. Investigation was based and implemented at scientific research laboratory of Traditional Medical Science Technology and Producttion Corporation and pathological laboratory of Institute Veterinary medicine. Chemically acting substances is steroid saponin content in Malbus compound was detected by using thin layerchromatography (TLC) and its value was determined by UV-spectrophotometry. The acute oral toxicity study was according to the method Prozorovsky (1978). The toxic nephrosis was produced using Gentamicin (80 mg/kg) rats by using Neugarten’s method (1983).ResultsThe ethanolic extract results of the phytochemical investigations showed that conteined in the “Malbus” determined the presence of bioactive substances such as flavonoids, steroid saponins. The ethanolic extract of Malbus was found to be LD50 of 16.3 g/kg. Toxic nephrosis was induced in Wistar rats administered preparation Malbus dose 80 mg/kg, 160 mg/kg, and 240 mg/kg. Blood levels of creatinine, uric acid, and urea were siginificantly reduced by Malbus treatment compared tocontrol. Histological study revealed that Malbus was effective for treatment of nephritis in rats induced by gentamicin.Conclusions:1. The ethanolic extract of “Malbus” compound determined the presence of bioactive substances such as flavonoids, steroid saponins.2. LD50 of Malbus 16.3 g/kg, the preparation is has nephroprotective effect on experimental Gentamicine induced nephrosis in rats.

BACKGROUND. The Mongolian traditional medicine Anar-5 is excellent for weak digestion and helps with stomach irritation, loss of appetite, and resulting body weakness. Anar-5 blends punicagranatum, cinnamomum cassia presl, piper longum, cardamom and alpiniaofficinarum. We are establish an experimental animal of chronic gastritis to investigate the effect of traditional medicine Anar-5 on rats gastric mucosa. Methods: In this study, the protective effect preparation in sixty five healthy, male wistar rats were treated with intragastric administration of ammonia water 0.1%. To rats in three experiments for 2 week, 4 week, and 6wk, gastric tissues were examined histopathologically for atrophic changes and blood’s gastrin produced by preparation treatment. Results: After the treatment of animals blood’s gastrin was significantly different from that in control group (p<0.05), and the gastric mucosal inflammation was infiltration of inflammatory cells, decreased thickness of lamina propria. Conclusion: Treatment with preparation from Anar-5 protectived by the chronic gastritis and gastric atrophy.

Species of the genus Iris (Iridaceae) comprises more than 1500 species and 14 species are growing in Mongolia. These are used in Mongolian traditional medicine espesially for treatment of cancer and inflammation. Various biologically active substances have been determined in genus Iris family of plants such as flavones and isoflavones, peltogynoids (Irisoid A, B, C, D and E), saponins, coumarins and benzoquinones. Iris ventricosa is one of the genus Iris family plants. The aim of the present study is to evaluate hepatoprotective effects of Iris ventricosa on carbon tetrachloride (CCl4)-induced liver injury in rats. The underground part of Iris ventricosa Pall was collected in September, 2011 in the botanical garden of Botanical Institute of Mongolia. Serum levels of aspartate aminetransferase, alanine aminetransferase, and alkaline phosphatase were determined. Histological study was also carried out. Aqueous and spirituous extracts (100 mg/kg) of Iris ventricosa significantly reduced levels of aspartate aminetransferase, alanine aminetransferase, and alkaline phosphatase in rats administered CCL4. Histopathological examination revealed that CCL4-induced changes in the liver were ameliorated by Iris ventricosa treatment. This is the first study that has shown the hepatoprotective effect of Iris ventricosa Pall in rats administered CCL4. The hepatoprotective effect of Iris ventricosa can be related to its potent antioxidative and anti-inflammatory activities. Neutralizing reactive oxygen species by nonenzymatic mechanisms and enhancing the activities of natural antioxidant enzymes of the liver may be the main mechanisms of the hepatoprotective effect of Iris ventricosa on CCl4-induced liver injury in rats.

Managchenmo is a traditional Mongolian medicine functional liquor comprising twelve crude drugs: Sus scrofa / cinis faecis /, Inula helenium L., Terminalia chebula Retz., Melia toosendan Sieb, Gardenia jasminoides Ellis, Lagotis integrifolia (Willd.)Schischk, Gentiana barbata Froel, Moschus moschiferus, Bezoar Bovis, Calcio sinteris, Carthamus tinctorius L,and Saccharum officinarum. Nowadays the Mongolian traditional herbs are more preferable than the chemical ones; for this reason studies on their use history and new technologies are conducted. Thus we study Managchinmo affects, herb for treatment of liver hot disorders, on trial rats (Handa.S.S, Sharma.A .1990 ) with hepatotoxicity. The levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkalinephosphatase (ALP) were determined through biochemical methods. The bile duct was cannulated with a tube to collect bile for two hours and bile flow was calculated by the weight of each specimen. Our results showed that Managchenmo (150 mg/kg) significantly prevented CCl4-induced changes in bile flow and serum levels of ALT, AST, and ALP. In conclusion, Managchenmo exerted a protective effect on CCl4-induced hepatotoxicity in rats.