Background: According to statistics from National Center of Public Health Development of Mongolia morbidity rate of cardiovascular diseases was 501.84 cases per 10000 population in 2006, which demonstrates increase of 2 times compare to 1996 and 50% increase compare to 2000. Goal: To study bio-ecological factors influencing on mortality and morbidity rate of prevalent cardiovascular diseases among Mongolians.Materials and Methods: We have chosen arterial hypertension, acute myocardial infarction, and chronic rheumatoid fever according 10th classification of WHO. Indices of morbidity, mortality of diseases were calculated per 1000 population.Result: Average atmospheric temperature t0С (χф2=1494); atmospheric pressure (χф 2=36.07); precipitation (χф 2=704); and mineralization of rivers (χф 2=532) have statistically significant effect on morbidity rate of arterial hypertension among the population of Uvurkhangai, Gobi-Altai, Darkhan-Uul, Gobisumber aimags and UB city. Average atmospheric temperature t0С (χф 2=241); atmospheric pressure (χф2=88.62); precipitation (χф 2=62.18); and mineralization of rivers (χф2=86.28) have statistically significant effect on morbidity rate of chronic rheumatoid fever among the population of Arkhangai, Bayankhongor, Umnugobi, Gobisumber aimags.Conclusions:1. Geographical distribution of arterial hypertension is prevailed in Uvur-Khangai, Gobi-Altai, Darhan-Uul, Gobi-Sumber aimags and UB city, distribution of acute myocardial infarction is prevailed in Hovd, Hubsgul, Arkhangai, Selenge, Gobi-Sumberaimags and geographical distribution of chronic rheumatoid fever is prevailed in Bayankhongor, Arkhangai, Umnugobi, Gobisumber aimags. 2. Ecological factors have statistically significant (χF 2=23.1-161.1) influence on the distribution of morbidity and mortality of arterial hypertension, acute myocardial infarction and chronic rheumatoid fever. Influencing ecological factors in regions covered with this study, such as temperature, atmospheric pressure, wind speed and mineralization of water sources have weaker and reverse correlations each separately (r=-0.1-0.2) but in combination they have medium and reverse correlations (r =-0.45- 0.52).3. The result of the research study established that morbidity and mortality of cardiovascular diseases (arterial hypertension, acute myocardial infarction and chronic rheumatoid fever) distributed in 3 regions on the territory of Mongolia depending on ecological factors.

1.Changes in microorganism’s quantity or activity can precede detectable changes in soil chemical properties, thereby providing an early sign of soil improvement or early warning soil degradation.2.Erdenet mining factory soil has approsintly 0.59*106 cell/g and control soil has 1.17*106 cell/g. Herein bacteria were 57%, actinomycete 31% and fungi 12%. But control soil bacteria were 83%, actinomycete 16% and fungi 1%.3.We have chosen deferent by morphology separate 29 cleanly cultures. In tolerance of heave metal, cultures which extracted from 1415 m level is tolerant against 6 mM of Fe, 5 mM of Pb, 3 mM of Mo and Cu, 1 mM of Cr

Introduction: In recent times, human tend use natural drugs which have low toxicity effect. We found out many drugs used in Mongolian and Tibetan traditional medicine.The genus Polygala (Polygalaceae) is represented in Mongolia by only three species (P.tenuifolia Willd, P.sibirica L, P.hybrida DC). These are well- known traditional Mongolian herbs, especially Tibetan medicine and modern medicine used for treatment of many diseases, such as prevent dementia and brain protection effects, antitumor activity and influenza, pneumonia, pharyngitis, heart disease and dysentery, and used as an antiphlogistic and for detoxification, expectoration. Some researchers have reported [4] phenols and triterpenes from this plant.In Mongolia, P.tenuifolia Willd, P.sibirica L, P.hybrida DC are distributed widely in various areas such as khangai, khentii, khubsgul at al.These plants are substituted by Tibetan lanceta (Lancea tibetica Hook.f.et Thoms. Scrophulariaceae. The Tibetan medicinal plant used for many disease in oriental medicine such as Tibetan, Chinese and Mongolian.Furthermore, this plant grows only in Tibetan autonomic region of China and Mongolia.The flowers, leaves and fruit are used in Tibetan and Mongolian medicine, they are said to have a sweet and bitter taste with a cooling potency. They are used in the treatment of pulmonary disorders. The fruit is used to treat heart disorders and retention of the menses, whilst the leaves are used for healing wounds.General Description of Lancea tibetica; Herbs, 3-10 cm tall, glabrous except for petioles. Rhizomes to 10 cm long, with a pair of membranous scales on each node. Leaves 6-10, rosulate; leaf blade obovate, obovate- ob-long, or spatulate, 2-8 cm long, subleathery, base tapering, margin entire or obscurely and sparsely toothed, apex obtuse and usually apiculate. Flowers in fascicles of 3-5 or in a raceme; bracts subulate- lanceolate. Calyx, leathery; lobes subulate-triangular. Corolla dark blue to purple, 1.5-2.5 cm long, tube 1-1.3 cm long; throat yellowish and/or with purple dots; lower lip middle lobe entire; upper lip erect, deeply 2-lobed. Stamens inserted near middle of tube; filaments glabrous. Fruit red to dark purple, ovoid, included in persistent calyx. Seeds numerous, brownish yellow, oblong.Future our experimental purpose is comparative study in chemical and biological activities these two plants, Polygala Hybrida DC & Lancea tibetica Hook.f.et Thoms which are grow in Mongolia.

Thyroid nodular lesions are the common clinical problem in the world. A variety of tests have been employed to separate benign from malignant thyroid nodules. These tests include isotope scanning and fine needle aspiration cytology (FNAC). Our research was based on the fact that the comparison of FNAC and thyroid isotope scan in thyroid nodule was not researched in Mongolia. Therefore, we want to evaluate the specimen adequacy of FNAC, and compare its result to thyroid isotope scanning in patients with thyroid nodule. The research was conducted on archive materials of FNAC and thyroid isotope scanning of patients with thyroid nodule who were treated in surgical department of The First Central Hospital from 2012 to 2015, and the statistic analyze was done by using SPSS 20 under the auspices of School of Pharmacy and Bio-Medicine,Department of pathology, Mongolian National University of Medical Sciences. We have collected the FNAC of 807 patients, including 34 patients (4.2%) were males and 773 patients (95.8%) were females. FNA cytology results were interpreted as benign in 495 cases (61.3%), follicular lesion of undetermined significance in 31 cases (3.8%), follicular neoplasm in 9 cases (1.1%) suspicious in 17 cases (2.1%), malignant in 3 cases (0.4%), and unsatisfactory in 252 cases (31%). We have got the result of thyroid isotope scanning of 43 (5.32%) patients out of 807 cases. On thyroid scan, 18 patients (41.9%) having cold nodule were labeled as suspicious for malignancy, 25 patients (58.1%) had hot nodule. The FNA diagnosis of 25 patients with a hot nodule following: 1 patient (4%) with neoplasm, 17 patients (68%) with benign results, 6 patients (24%) had non-diagnostic. The FNA diagnosis of 18 patients (41.9%) with cold nodule following: 1 patient (5.5%) with follicular lesion of undetermined significance, 10 patient (55.5%) with benign, 5 patient (27.7%) with non-diagnostic, 2 patient (11.1%) had malignant. The FNAC results were interpreted as benign in 495 cases (61.3%), malignant in 3 cases (0.4%), and unsatisfactory in 252 cases (31%). Two patients out of 18 patients with cold nodule diagnosed as malignancy by FNAC. The sensitivity and specificity of thyroid isotope scanning was 96% and 16.6%, respectively.

Background: Soil pollution in Ulaanbaatar has increased in recent years, causing infectious and non-communicable diseases, both benign and malignant.
Therefore, in order to reduce soil pollution, it is necessary to isolate strains resistant to heavy metals and use these microorganisms capable to degrade or accumulate heavy metals for bioremediation purposes. Methods: Bacterial pure cultures were isolated from soil and water samples and incubated in a Nutrient Broth medium with a lead concentration of 1000 mg/L in a thermostat with a shaker at 37°C. The growth rate was determined at a wavelength of 600 nm using a Spectrophotometer. Results: In total 69 pure cultures were isolated from the soil samples and 65 pure cultures from them were able to grow at the concentration of lead of 1 mmol/L. 4 pure cultures (UBLF1, UBLF3, UBMF2, and SUBZ4) were able tolerate lead concentrations up to 8 mM. These cultures were determined by semi-automatic VITEK®2 analyzers and identified as Bacillus sp. (83%) and UBMF2 identified as Bacillus thuringiensis (93%) respectively. Conclusion The lead content in water samples were found to be acceptable, and isolated pure cultures were not able to withstand the lead concentrations.
Pure culture UBLF3 isolated from the soil capable to reduce lead concentration (500 mg/L) up to 302 mg/L and 230 mg/L in the medium, and pure culture UBMF2 up to 340 mg/L and 279 mg/L respectively

BackgroundIn Mongolia scientists have been doing research about skin disease and process of aging. Howeverthere has not enough study material about skin aging process which is the key indicator of aging andhow that affect to Mongolian people.GoalMain purpose is to study correlations between main index of skin type of the body, cardiovascularsystem, biological age, epidermal growth factor and blood plasma melatonin, within the people of Uvsprovincial center as they are relatively healthy people.Material and MethodsResearch works were done using cross sectional study model in years 2015-2016, within randomlychosen 400 citizens of Ulaangom city, counted to be relatively healthy people. Research model includes41 questionnaires, examination and blood plasma samples.Basic indexes of the body, cardiovascular system and respiratory functions were chosen by biomarkers,biological age is determined by V.P.Voytenko’s 4th version method and adaptive capacity assessment isdetermined by R.M.Baevskii’s multi-repeating regression method.Main skin type parameters such as porosity, wrinkles, pigmentation, elastics and aging is determined bythe “BOOMTECH skin diagnosis” digital equipment made in the Republic of Korea.The blood plasma MLT and EGF were measured by the method of ELISA analysis ELISA made in thelaboratory of the “AVITSENNA Science and Technology center”.The study material analysis carried out an analysis using logistic regression analysis and Pearson’scorrelation coefficient with SPSS 23.0 software.ResultThere’s an inverse weak correlation between blood plasma MLT and EGF with body wealth characteristicsof Uvs province citizens. It shows that when the blood plasma MLT and EGF decreases the body weight,body height and body weight index increase tendency.In women the decrease of blood plasma MLT and EGF also decreases the body height and body weightindex.Research on correlation between cardiovascular system and blood plasma MLT and EGF shows thatdecrease of blood plasma MLT causes in increased arterial pressures in both sexual groups, decreasedEGF in women causes decreased arterial pressure.Correlation study between skin main characteristics and blood plasma MLT and EGF shows thatdecreased blood plasma EGF in men resulted in decreased skin wrinkles, porosity, elasticity and skin aging index, in women decreased skin pigmentation and elasticity, and increased skin wrinkles, porosity and aging index in women.Research study of correlation between biological age and adaptive capacity assessment with the blood plasma MLT and EGF shows that decreased MLT and EGF in men results in increased biological age characteristics. In other words, the early aging is caused.The logistical regression analyses made using blood plasma melatonin, skin porosity, biological age,body height and sex as the risk parameters of Pearson coefficient and chi-squared parameter. Above model resulted in skin porosity and blood plasma MLT’s are becoming independent variable assessing the skin aging.Conclusions:1. The decreased MLT and EGF’s are resulted in increased body weight, body height and body weight index in men and decreased body height, body weight index in women.2. Decreased blood plasma MLT resulted in increased arterial pressure in both sexual groups and decreased EGF resulted in decreased arterial pressure in women.3. The decreased blood plasma MLT and EGF results in decreased skin wrinkles, porosity, elasticityand skin aging parameter with the increased skin pigmentation in men, as it results in decreasedskin pigmentation, elasticity and skin porosity parameters with the increased skin aging degree in women.4. When epidermal growth factor and blood plasma melatonin is decreased in men it results in increased biological age, but depends directly to the adaptive capacity assessment.5. Skin porosity and blood plasma melatonin were becoming independent variable to assess skin aging.

BACKGROUND. Uterine cervical cancer is the fourth leading cause of cancer deaths in women worldwide. In our country, cervical cancer is second most common cancer in women. Uterine cervical smear (Papanicolaou test) remains an effective and widely used method for early detection of precancerous and cancerous lesions. Since 2002, the cervical smear was introduced to the clinical practice of our country. However, there is no study to performed external quality assurance of cervical smear until now. MATERIALS AND METHODS. We selected 20 glass slides of uterine cervical smear, the diagnosis was approved by histopathology. Each chosen slides were evaluated by four cytologists of A, B, C, D hospitals with hidden clinical information, independently. RESULTS. The sensitivity of A, B, C and D hospitals were 87.5%, 93.3%, 93.3%, and 93.3%, respectively. The specificity of A, B, C and D hospitals was 85.7%, 85.7%, 75%, and 66.6%, respectively. The diagnostic concordance of A, B, C and D was 70%, 75%, 50%, and 55%, respectively. The agreement of cytological diagnosis was moderate (kappa = 0.55), moderate (kappa = 0.43), fair (kappa = 0.37), and fair (kappa = 0.33) in A, B, C, and D hospitals, respectively. CONCLUSION: The external quality assurance in cytopathology is needed in Mongolia. The diagnostic concordance method would be applicable in our country to improve diagnostic agreement.

Background: Weight at birth is a good indicator of the mother’s health and nutrition status during gestation and a child’s chances for survival, growth, long-term health, and psychosocial development. Low birth weight (defined as less than 2500 grams) poses a range of serious health risks for children. Objective: To assess the prevalence of low birth weights (LBW). Materials and Methods: The NNS V was implemented in 21 provinces (aimags) in 4 economic regions (Central, Eastern, Khangai, Western) and the capital city of Ulaanbaatar. A total of 2250 children aged 0-59 months. Given the regional differences in lifestyle and nutrition status, target populations were stratified into 5 strata based on economic region and Ulaanbaatar with equal samples drawn from each stratum using a cluster-randomized sampling design. For the selection of households in urban areas, the process involved first selecting 30 khoroos (clusters), then khesegs, and then households with a child 0-59 months of age. Ethical considerations : The survey methodology was discussed at the Scientific Committee of the Public Health Institute (recently named by National Center for Public Health) and granted the PHI Directors Order on 28th June, 2016. Ethics approval for conducting the NNS V, was obtained from the Medical Ethics Committee under the Mongolian Ministry of Health. Results: Almost all (99.5%) children in the sample were weighed at birth with 5.0% weighing less than 2500 grams at birth and 12.6% weighing over 4000 grams at birth. The prevalence of low birth weight was slightly higher among girls than boys, however high birth weight was much more common in boys (15.9%) compared to girls (9.5%). Prevalence of low birth weight was highest in Western region (9.6%) and the poorest households (7.8%) while the prevalence of high birth weight was highest in Ulaanbaatar (13.8%) and wealthiest households1 (16.4%). High birth weight was more than double among children of overweight (17.5%) and obese (17.3%) mothers compared to children of normal weight (8.4%) or underweight (7.0%) mothers. Among singleton births, women <29 years of age had higher LBW rates than did those 30-39 years of age in both years, irrespective of birth order. LBW rate was more than double among mothers in kazak ethnic groups (13.8%). Conclusions The younger age of the mother was more likely to develop low birth weight, and it was twice as high among mothers in Kazak ethnic groups. Therefore, adolescents and women of reproductive age needed to improve the health education and to identify the causes and risk factors of low birth weight among mothers in Kazak ethnic groups.

Background: Specifically, stomach cancer ranks as the fifth leading cause of cancer morbidity and mortality worldwide. Early-stage detection significantly improves survival rates, with over 90% of patients diagnosed at stages I and II living beyond five years. To improve the early detection of gastric cancer, it is necessary to complement the conventional method of endoscopic examination with biomarker analysis. We aimed to compare biomarkers such as pepsinogen C (PGC), matrix metalloproteinase 2 (MMP2), matrix metalloproteinase 9 (MMP9), and the cell proliferation marker Ki-67 with immunohistochemical analysis. Purpose: A comparative study and evaluation of biomarkers for the early detection of gastric cancer. Materials and Methods: The study was conducted using a retrospective cohort design. Research ethics issues were discussed at the meeting of the Medical Ethics Control Committee of the Ministry of Health on October 13, 2023, and permission to start the research was obtained (Resolution No. 23/051). The information was gathered based on the criteria for K29.3, K29.4, K31, and C1 diagnoses according to the international ICD 10 classification, and participants were selected accordingly. Proteins such as PGC, MMP2, MMP9, and Ki-67 were examined using a tissue microarray kit and evaluated through immunohistochemical analysis. Results: Negative gastric tumor markers PGC, Ki-67, MMP2 and MMP9 were evaluated by immunohistochemical analysis. The mean PGC protein staining values were 6.20±2.61 for chronic superficial gastritis, 5.45±2.47 for atrophic gastritis, 3.61±2.0 for metaplasia, and 3.31±1.75 for gastric cancer, with statistically significant differences between the groups (P<0.001). The mean Ki-67 protein staining values were 0.1 ± 0.4 for chronic superficial gastritis, 0.33 ± 0.55 for atrophic gastritis, 0.09 ± 0.39 for metaplasia, and 2.62 ± 0.78 for gastric cancer, also showing statistically significant differences (P<0.001). The mean MMP2 and MMP9 protein staining values were 0.2±0.76 and 1.2±2.04, respectively, for chronic superficial gastritis; 0.28±0.52 and 3.28±2.82 for atrophic gastritis; 0.35±1.04 and 1.12±1.45 for metaplasia; and 1.38±2.11 and 5.29±2.51 for gastric cancer, with all differences being statistically significant (P<0.001). Conclusion PGC protein, a negative tumor marker, decreases during the transition from a gastric cancer precursor to cancer. MMP2 protein, a marker of cell migration and metastasis, has little diagnostic value, while the expression of MMP9 and the Ki 67 are highly effective in gastric cancer. Immunohistochemical analysis of endoscopic biopsy tissue to detect the negative tumor marker PGC, the positive marker Ki-67, and MMP9 can be used for early detection of gastric cancer.

Background: Overweight and obesity are conditions of over nutrition resulting from consumption of more calories than the body requires leading to excess body fat accumulation. The prevalence of both overweight and obesity is increasing globally in all age groups in high, middle, and low-income countries and is largely attributed to the “nutrition transition” with a shift from traditional diets to readily available and inexpensive high-energy and low-nutrient foods [1].
The move away from traditional to “Junk” foods often occurs at the same time as the move of populations away from daily physical activity and increase in sedentary activities such as time spent in front of a phone, computer, or television, furthering increasing the risk of becoming overweight. People who are overweight or obese are at higher risk for serious health problems including hypertension, heart disease, stroke, diabetes, some cancers, and osteoarthritis. The increase in overweight and obesity is accompanied by a dramatic increase in prevalence of these chronic diseases at the global level. While the long-term repercussions of overweight and obesity are severe, they are largely preventable and treatable through healthy diets and healthy lifestyle behaviors [2]. Materials and Methods: The NNS V was implemented in 21 provinces (aimags) in 4 economic regions (Central, Eastern, Khangai, and Western) and the capital city of Ulaanbaatar. Given the regional differences in lifestyle and nutrition status, target populations were stratified into 5 strata based on economic region and Ulaanbaatar with equal samples drawn from each stratum using a cluster-randomized sampling design [3]. Total participated 1944 mothers’ years of age who had a child aged 0-59 months. Ethical considerations : The survey methodology was discussed at the Scientific Committee of the Public Health Institute (recently named by National Center for Public Health) and granted the PHI Directors Order on 28th June, 2016. Ethics approval for conducting the NNS V, including obtaining biological (blood and urine) samples, was obtained from the Medical Ethics Committee under Ministry of Health, Mongolia on 7th July, 2016 with granted approval to conduct the survey and send blood serum samples to the VitMin laboratory in Germany. Participation in the survey was voluntary and oral and written informed consent was obtained from all individuals selected for the survey. Participant confidentiality was maintained during data collection, data entry, data analysis, and in the dissemination of survey findings. Results: Nearly half of mothers (46.2%) were overweight with 16.5% of mothers obese and 4.1% underweight. The prevalence of overweight in mothers was highest in rural areas (50.1%), married status (49.3%), and lowest in Ulaanbaatar (42.3%) compared to other regions (48.6% to 52.6%). The overall prevalence of anaemia among mothers was 16.2% with highest prevalence amongst Kazakh (31.9%) and other ethnic minority mothers (25.1%), mothers with secondary education (20.3%) and unemployed mothers (19.9%). Minimum dietary diversity is a proxy indicator for the nutrient content of a diet based on consumption of at least 5 of 10 food groups.¹ People who consume food items from at least 5 food groups are likely to consume at least 1 animal-source food and items from 2 or more of the fruit and vegetable food groups. In the NNS V, 70.2% of mothers met the minimum dietary diversity requirement with a higher prevalence in urban areas (74.8%) and Ulaanbaatar (77.5%). Conclusion: The prevalence of overweight and obesity in mothers of reproductive age is 46.2%, with the highest percentage of mothers with overweight and obesity being in rural areas (50.1%), which is 5.8 percentage points higher than that of urban mothers. 16.2% of mothers are anemic. Prevalence of Minimum dietary diversity among mothers is 70.2%. Food consumption is inadequate in mothers with the primary and secondary education, and in mothers in rural areas, and in poorest mothers or in mothers with the second and third wealth index. ¹FAO and FHI 360. 2016. Minimum Dietary Diversity for Women: A Guide for Measurement. Rome: FAO.