Background: Peripheral blood stem cells are today the mains source of cells for transplantation. The number of CD34 in peripheral blood of adult healthy volunteers is very low, so we have to mobilize. Objectives: To apply a protocol to collect CD4 cells in peripheral blood of normal donors mobilized by using G-CSF. Subjects and method: G-CSF (Leukokin) at a dose 10 mcg/kg/day was injected subcutaneously in 5 consecutive days for mobilization CD34 from 5 healthy donors. Count the number of CD34 everyday and collect CD34 cells in 5th \ufffd?6th day by automated blood cell separator (COBE-Spectra), observed clinical and hematilogy, biochemistry symtoms spontaneously. Results: The number of CD34 harvested is 215,5 \ufffd?570,2 x 106 CD34/donor. Almost other of parameters of hematology and biochemistry of donors were normal after one week from the last separation. Conclusion: Mobilization with G-CSF and aphaeresis of periphral blood stem cell from normal donors is feasibility and safety. The number of CD34 can be to allograft for an adult patient.
Hematopoietic Stem Cells ; Granulocyte Colony-Stimulating Factor ;

Background: Umbilical cord blood - Stem cell CD34 are attracted to study and apply to cure many serious diseases. But the quantity of the cell is small, so it only used to children. Therefore, studying to find out the high effect collected process is necessary. Objective: studying to find out the high effect process of collecting hematopoietic stem cell from umbilical cord blood. Subjects and methods: the study included 112 umbilical cord blood specimens collected from healthy mother delivering at National Hospital of Obstetric and Gynecology.Results: The condition of the mother was healthy, with no risk factors of infection or genetic disease. The average age of mothers was 28 \xb1 5 years old, average weight: 63 \xb1 5 kg. Age of fetus was from 39-41 weeks, average weight: \ufffd?3000g. Collection technique: cutting the umbilical cord after delivery 6-10 seconds, cutting the umbilical cord far from neonatal 6 cm, blood collection continued after the collection of placenta, all processes must be implemented rapidly in the 4 -5 minutes in aseptic condition. With the above process it can be obtained with high effect blood: volume was 90 - 120ml (48%). CD34 cell count: 1.6 \xb1 0.3x106, the number of eukaryotic cells: 13.53 \xb1 1.7x108/unit of the umbilical cord. Conclusions: The process can take the large volume of umbilical cord blood, which can be used for children and low weight adult. \r\n', u'\r\n', u'\r\n', u'
Fetal Blood

Background: The reduction of erythrocytes from cord blood is very need for long - term storage of C034 cells for transplantation. Reduced erythrocyte will reduces preservative blood volume, preservatives and freely HST when defrosting, so stem cells are better protected. Objectives: To study selection of the best centrifugal procedure to reduce maximal erythrocytes and lose minimal C034 cells from cord blood. Subjects and methods: 20 blood samples selected from 60 cord blood units was used for this study. The study was carried out through two steps. In the first step, the centrifugal speed was fixed and the centrifugal time was changed.In the second step, the centrifugal time was fixed, the centrifugal speed was changed. From collected results the best appropriate procedure to reduce erythrocytes from cord blood have been selected. Results: The procedure of gradient centrifuge with speed of 500g in 6 minutes isolated> 50% of erythrocytes, kept > 84% of CD34 cells and then centrifuge of 1000 g in 10 minutes reduced about 40% of volume of nuclear cell - suspension. Conclusion: The procedure can use for preparation of stem cell suspension from cord blood to storage in nitrogen liquid. \r\n', u'\r\n', u'
Erythrocytes/ pathology ; Fetal Blood/ chemistry ; drug effects ; immunology

Background:The progress of the cardiac intervention technique has been changed the doctors' viewpoint in the treatment for congenital cardiac. In the past years, treatment for congenital cardiac needed to surgery but nowadays, the cardiac intervention technique are being applied in many Cardiac Centers with very good results. This technique also helped to prevent the complication in cardiac operation. Objectives:This study aims to report the results for cardiac intervention in National Hospital of Pediatrics. Subjects and method:A prospective study was conducted on 298 children diagnosed with patent ductus arteriosus, atrial septal defect (ASD), ventricular septal defect, coarctation, pulmonary stenosis (PS), aortic stenosis (AS), small patent ductus arteriosus (PDA) infant with pulmonary atresia - ventricular septal defect (VSD) and Dextro- transposition of the great arteries (d- TGA) with intact ventricular septum at National hospital of Pediatric between June 2004and October 2006. Results: Transcatheter closure of PDA, ASD by Amplatzer or Coil was safe and effective. Critically ill children diagnosed with PS, AS and Coarctation may be saved by valvuloplasty and angioplasty procedures. Catheter interventions avoided the needs for surgery without scars. Catheter interventions had low complications. Conclusion: The hospitalized duration was short so the expenditures reduced.
Heart Defects ; Congenital/ epidemiology ; therapy

Purpose: Coronavirus disease 2019 (COVID-19) pandemic has spread worldwide rapidly and patients with cancer have been considered as a vulnerable group for this infection. This study aimed to examine the expressions of angiotensin-converting enzyme 2 (ACE2) and transmembrane serine protease 2 (TMPRSS2) in tumor tissues of six common cancer types. Materials and Methods: The expression levels of ACE2 and TMPRSS2 in tumors and control samples were obtained from online databases. Survival prognosis and biological functions of these genes were investigated for each tumor type. Results: There was the overexpression of ACE2 in colon and stomach adenocarcinomas compared to controls, meanwhile colon and prostate adenocarcinomas showed a significantly higher expression of TMPRSS2. Additionally, survival prognosis analysis has demonstrated that upregulation of ACE2 in liver hepatocellular carcinoma was associated with higher overall survival (hazard ratio, 0.65; p=0.016) and disease-free survival (hazard ratio, 0.66; p=0.007), while overexpression of TMPRSS2 was associated with a 26% reduced risk of death in lung adenocarcinoma (p=0.047) but 50% increased risk of death in breast invasive carcinoma (p=0.015). Conclusion There is a need to take extra precautions for COVID-19 in patients with colorectal cancer, stomach cancer, and lung cancer. Further information on other types of cancer at different stages should be investigated.

Purpose: Coronavirus disease 2019 (COVID-19) pandemic has spread worldwide rapidly and patients with cancer have been considered as a vulnerable group for this infection. This study aimed to examine the expressions of angiotensin-converting enzyme 2 (ACE2) and transmembrane serine protease 2 (TMPRSS2) in tumor tissues of six common cancer types. Materials and Methods: The expression levels of ACE2 and TMPRSS2 in tumors and control samples were obtained from online databases. Survival prognosis and biological functions of these genes were investigated for each tumor type. Results: There was the overexpression of ACE2 in colon and stomach adenocarcinomas compared to controls, meanwhile colon and prostate adenocarcinomas showed a significantly higher expression of TMPRSS2. Additionally, survival prognosis analysis has demonstrated that upregulation of ACE2 in liver hepatocellular carcinoma was associated with higher overall survival (hazard ratio, 0.65; p=0.016) and disease-free survival (hazard ratio, 0.66; p=0.007), while overexpression of TMPRSS2 was associated with a 26% reduced risk of death in lung adenocarcinoma (p=0.047) but 50% increased risk of death in breast invasive carcinoma (p=0.015). Conclusion There is a need to take extra precautions for COVID-19 in patients with colorectal cancer, stomach cancer, and lung cancer. Further information on other types of cancer at different stages should be investigated.

In this study, we report the manganese peroxidase production ability from a Fusarium sp. strain using an inexpensive medium of agriculture residues of either rice straw or wood chips as carbon source. The highest manganese peroxidase activity on rice straw medium and on wood chips was 1.76 U/mL by day 9 and 1.91 U/mL by day 12, respectively.
Agriculture ; Carbon ; Fusarium* ; Manganese* ; Mass Screening* ; Peroxidase* ; Wood