Objective To optimize the extraction procedure for Anmai Granules. Methods With the content of Chlorogenic acid and Cinnamylic acid as indexes, orthogonal table L9(34) was used in the experiment of extracting in alcohol to study the effect of technological parameters (the concentration and dosage of alcohol, extraction times, the time of extraction) on the content of Chlorogenic acid and Cinnamylic acid. Results The optimum process is that 70% alcohol is used as extracting solution adding 9 times amount of solution, extracting 2 times, 1.0 hours for each time. Conclusion The extraction process is stable and suitable for industrial production.

The optimum conditions for the processing of Sparganium stoloniferum Buch-Ham. withvinegar were studied by L9(3)4 orthogonal experimental design, as guided by decrease of writhing rate inanalgesic test and anticoagulant activity. The results showed that the best processing conditions were stirfrying of the raw S. stoloniferum with the addition of 25 kg of vinegar per 100 kg of crude drug at 130℃for 10 min.

Objective To establish a method for determination of puerarin content in Jingning capsule. Methods The column was Kromasil (250 mm?4. 6 mm, 5 ?m). The mobile phase consisted of methanol- 0.2% water H3PO4 (20/80), with flow rate of 1 mL/min and the UV detective wavelength set at 250 nm. Results Puerarin showed a good linear at the range of 6.268～125.360 ?g/mL (r=0.999 8). The average recovery was 101.42%, with RSD of 1.22%. Conclusion This method is accurate, simple, repeatable and suitable for the determination of puerarin content in Jingning capsule.

Objective:To compare the effect of different processing methods on the content of Ginsenoside Rg 1 from Radix Notoginseng. Methods: The HPLC method was used for the determination. Chromatographic condition includes: C 18 column, acetonitrile water (45∶55) as a mobile phase, UV detector wavelength at 203nm, 25 ?C column temperature and 0.7 mL?min -1 of flow rate. Results: The content of Ginsenoside Rg 1 of the Radix Notoginseng powder was the highest among all samples. Its recovery was 95.53% and RSD was 2.11% . The correlation coefficient was 0.9999 . Conclusions: It is better to use Radix Notoginseng in the form of the Powder in clinic.

Objective: To explore the effect of processing on the content of tetrahydropalmatine in Rhizoma Corydalis. Methods: HPLC was used with chromatographic condition included: C 18 column, mobile phase of methanol water phosphate buffer (70∶30∶1.8) and detection wavelength at UV 280nm. Results: The content of tetrahydropalmatine in the Rhizoma Corydalis processed with vinegar is the highest among all processed products. The recovery is 99.07% and RSD =1.81%, respectively. Conclusion: The processing with vinegar can increase tetrahydropalmatine content of Rhizoma Corydalis.

AIM: To research the best processing method of Rhizoma Curcumae with water.METHODS: L 9(3 4) orthogonal table are decided with three effects: moistening time, steaming time, thickness of processed pieces. According to the amount of curcumin and volatile oil, orthogonal Design is applied to water processing Rhizoma Curcumae.RESULTS: The best processing method is: Rhizoma Curcumae is moistened for 20min, then cutting up to 3mm thick after steaming 30min.CONCLUSIONS:The steaming time has no obvious effect on the amount of curcumin in Rhizoma Curcumae, but if the amount of volatile oil is taken as marker. the steaming time has obvious effect.

AIM: To study the blood quickening and stasis dispelling effect of the different processed products of Rhizoma Curcumae by rat's platelet aggregation in vivo and its hemorrheological properties and blood coagulation. METHODS: The platelet aggregation determination, hemorrheological properties determination and mice blood coagulation method were used to observe the blood quickening and stasis dispelling effect of the different processed products of Rhizoma Curcumae. RESULTS: The different processed products of Rhizoma Curcumae all had some inhibition on platelet aggregation, anticoagulation and improvement on the hemorrheological parameters. Among all of them, the processed product with vinegar was the most strong. CONCLUSION: In traditional processes, pharmacological test shows that Rhizoma Curcumae processed with vinegar has the best effects.

Objective: To determine puerarin content in Cuochanglin Granules by HPLC. Methods: The determination was performed on C 18 column with MeOH H 2O(25∶75) as a mobile phase by means of external standard method. Results: There was a linear relationship between puerarin and chromatographical peak area when puerarin concentration was at a range of 0.099?g～0.495?g. Conclusion: The method is simple, accurate and sensible, and can be used for quality control of this preparation.

OBJECTIVE:To establish the quality standards for Fufang ningshen granule. METHODS:TLC was used for the qualitative identification of Radix angelicae and White paenoy;HPLC was adopted for the contents determination of spinosin and verbascoside:the column was Kromasil C18 with the mobile phase of acetonitrile-0.1% acetic acid(16∶84,V/V)at a flow rate of 1.0 ml/min,detection wavelength was 330 nm,column temerpature was 30 ℃. RESULTS:The TLC spots of R. angelicae and W. pae-noy were clear with good separation,negative control without interference. The linear range was 0.055 44-0.277 2μg for spinosin(r=0.999 4)and 0.055 98-0.279 9 μg for verbascoside(r=0.999 5);RSDs of precision,stability and reproducibility tests were lower than 3.0%;recoveries were 95.62%-100.53%(RSD=1.77%,n=9) and 95.63%-102.57%(RSD=2.74%,n=9). CONCLU-SIONS:The standard can be used for the quality control of Fufang ningshen granule.