1.Transbronchial lung biopsy via the fibreoptic bronchoscope \r\n', u'in diagnosis of the lung diseases \r\n', u'
Hanh Thi Chu ; Phuong Thu Phan ; Giap Van Vu ; Chau Quy Ngo ; Tuan Tri Ngo
Journal of Medical Research 2007;53(5):56-60
Background: Bronchoscopy is an essential technique for diagnosis of some lung diseases by taking samples for histological and cytological tests. Objective: To assess the role of transbronchial lung biopsy procedure in diagnosis some parenchymal pulmonary diseases. Subjects and method: A retrospective study conducted in 50 patients with diffuse and localized lung diseases, who were admitted to the Department of Respiratory in Bach Mai Hospital, from January 2003 to December 2005. Results: Transbronchial lung biopsy confirmed the diagnosis in 30 of 50 (60%) cases; with localized malignant lesions (12%), diffuse lung disease (16%), tuberculosis (28%), and cystic fibrosis (4%). Following transbronchial lung biopsy, two patients had pneumothorax (1 patient had chest tube drainage) and five patients had hemoptysis but no of them required further treatment. Conclusions: Transbronchial lung biopsy offer good diagnostic accuracy and low complication rate.
Bronchoscopy/ contraindications
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Lung Diseases/ diagnosis
2.Result of production of standard Albumin from human plasma.
Phan Trung Do ; Duong Tuan Pham ; Hien Thi Do ; Ha Diem Vo ; Thuy Thi Nguyen ; Thin Duy Ngo ; Phuc Hanh Hoang ; Tri Anh Nguyen
Journal of Medical Research 2007;52(5):1-6
Background: Human albumin was produced and used in many countries. Cohn's technique had been used to precipitate albumin from human plasma. This technique was easy and cheap and the quality of the product was good. In Vietnam, human albumin had to import, but the prices was very expensive. Vietnam was having good plasma in large quantity and high quality. That\u2019s why research on production plasma albumin was essential.\r\n", u"Objectives: This study aimed at using Cohn's technique improved by Drohan and Van - Aken to produce standard albumin from human plasma. Subjects and method: Human plasma detected VIII-factor was used for present study. Plasma \ufffd?albumin was precipitated by ethanol at low temperature and pH. The collected albumins have been liophilizated and storage at 40C. The quality and quantity of Albumin was evaluated by quantitative analysis and protein \ufffd?electrophoresis. Results: The 418g of albumin powder was produced from 16 liters of plasma detected F \ufffd?VIII. The quality of this albumin come up to standard (>95%) and quantity of albumin collected from one liter of this plasma was 26g. Conclusion: In the Vietnamese condition, the technique of Cohn can be used to produce standard albumin for treatment.\r\n", u'
Albumins/ standards
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Plasma
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3.Applied research into the production process of gama - Globulin from Human Plasma and Standard gama - Globulin with Rich of Anti \u2013 HBs
Phan Trung Do ; Duong Tuan Pham ; Hien Thi Do ; Thuy Thi Tran ; Thin Duy Ngo ; Phuc Hanh Hoang ; Hai Xuan Le ; Tri Anh Nguyen
Journal of Medical Research 2008;0(1):67-71
Introduction: The need for gama \u2013 globulin, especially gama - globulin - anti \u2013 HBs, is huge in Vietnam. A number of patients cannot to afford use them due to the high price as they are imported. Meanwhile, Vietnam has high quality input sources for producing gama \u2013 globulin. \r\n', u'Objectives: To study the production process of gama - Globulin from Human Plasma and Standard gama - Globulin with Rich of Anti \u2013 HBs. \r\n', u'Subjects and method: 168 samples of human plasma from voluntary blood donors, which had been screened with for transfusion transmittable infections (TTLs), were chosen as plasma with rich of anti - HBs. The plasma with anti - HBsAg was precipitated with ethanol 25%, pH 6,9 to gain gama - globulin with rich anti - HBs, which was dried by Dutch Ly - 3 - TTE machine. Activation of anti - HBs gama - globulin was identified by a standard method of the degree of antibody specific for anti - HBsAg kit of BIORAD, \r\n', u'Results: The purity of the gama - globulin achieved was 93%, which was almost equal with the results of some foreign researchers (7.8), the activation of anti - HBs was 1:128 dilution degree. The productivity of gama - globulin gaining from 1 litter plasma was 6.0 gram. This new issue was first demonstrated in Vietnam. \r\n', u'Conclusion: We can domestically produce anti-Bs gama - globulin with high degree of activeness (1:128) from human plasma by the precipitating method with ethanol, pH and low temperature. \r\n', u'
Human plasma
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gama - globulin - anti - HBs
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Precipitation of ethanol
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pH and low temperature