Introduction: Endothelial progenitor cells (EPC) have a role in the maintenance and promotion of vascular repair and are negatively correlated with coronary atherosclerosis. Goal: To culture of EPC-CFUs during coronary atherosclerosis, evaluate endothelial nitric oxide synthetase (eNOS) enzyme levels in the culture. Materials and Methods: The 10 ml blood was drawn from the peripheral vein of 12 man patients that stable angina 4, acute myocardial infarction (AMI) 4 and healthy people 4. Peripheral blood mononuclear cells were isolated by Ficoll density-gradient centrifugation and EPC-CFUs was assayed after two platings and a 6 day culture on fibronectin coated, 72 well plates, as described. eNOS enzyme titers were determined by ELISA according to the protocol in the cells culture. Results: The people were 52±2.12 years. The number of EPC-CFUs increases with accordance of patients with stable angina, AMI, healthy people with the statistical significance (F=17.3, p<0.001): stable angina (2.6±0.47 colony/well), AMI (6.7±0.81 colony/well), healthy people (10.5±1.34 colony/well). Furthermore, ANOVA test of eNOS enzyme levels in patients with stable angina (5.2±0.61 pg/ml), AMI (8.7±1.49 pg/ml) and healthy people (13.7±2.48 pg/ml). The significant difference (F=6.2, p=0.003) was observed among the three groups. The number of EPC-CFUs had direct significantly correlation (r=0.621, p<0.001) with the eNOS enzyme levels of this culture. Conclusion: Number of EPC-CFUs and eNOS enzyme levels decrease at patient with stable angina, indicate more than endothelial dysfunction. Ethical approval The ethics committee of Mongolian National University of Medical Sciences (ID: 6/3/201506, approved on Jan 01, 2015)

Introduction: Accurate measurement of multiple cytokines concurrently is essential for comprehensively understanding immune responses in various diseases. Goal: To develop a technological methodology for the simultaneous quantification of multiple cytokines in mouse serum using a flowcytometry Materials and Methods: Utilizing a C57BL/6 mouse model, we induced cytokine production by intraperitoneal injection of alpha-galactosylceramide (α-GalCer) and analyzed cytokine levels 24 hours later using a MACSQuant analyzer 10 equipped with LEGENDplex™ Mouse Th Panel (13-plex) assay. Means between groups were compared using the independent sample t test, and statistically significant differences were considered at p ≤ 0.05. Results: Our results demonstrate that the emitted intensity increased proportionally with standard concentration, with slightly elevated cytokine staining observed for MCP-1 and IL-10.
The concentration of each cytokine was determined using a regression equation based on the mean fluorescence intensity value of the cytokine in each standard. Importantly, we observed significantly highest levels of IFN-γ (p<0.05) in α-GalCer-injected mice, indicating successful induction of cytokine production, particularly from NKT cells. Notably, our investigation revealed that levels of IFN-b, IL-17A and IL-27 in α-GalCer-injected mice increased (p<0.05) those of other cytokines, compared to negative controls. The determination of multiple cytokines simultaneously via flowcytometry, facilitated by the LEGENDplex assay, holds significant importance in enabling comprehensive comparison of cytokine concentrations. Conclusion An experimental methodology for the simultaneous determination of multiple cytokines by flowcytometry was successfully developed.