Recently, it has been done many phytochemical and pharmacological studies about raw tissues in TSP (Three seed prescription). The purpose of this study was to determine the effect of TSP on lipid peroxidation versus physiological antioxidants including SOD, GSH-Px and CAT in vitro in rat model with blood heat disease associated with “Three hot’. Compare to the control group MDA was significantly increased blood heat model. All three antioxidant ferments are clearly increased by “Three hot’. Also, it was noticed strong correlation between red cell aggregation index and MDA level. These results suggested, that “Three hot’I disturbed oxidant and antioxidant balance in blood heat models, which leads to red cell aggregation. On the basis of these results we conclude TSP could recover lipid peroxidation process by increasing antioxidant ferments