Mongolia has a long history of traditional medicine and it is greatly related with the nomadic culture that has been developed along with lifestyle, diet, and animal-husbandry works. Traditional Mongolian Medicine (TMM) is based on Buddhist philosophy, cosmo-energical teachings in 5 elements. It belongs to the Eastern medicine which originated from Indian and Tibetan traditional medicine. There is wide range of theory and conception source in Mongolian traditional medicine, which needs scientific explanation. Important branches of scientific study might be divided into following topics: theory (to gather information from old literature and study fundamental principles of traditional medicine), phytochemistry (strives for obtaining active principles from natural medicinal materials and standardize new preparations), pharmacology (to study pharmacological activity and mechanisms), exploration and cultivation of medicinal plants. Drug technology research activities have been intended mainly to produce new medicaments based on traditional recipes. During redevelopment period of traditional Mongolian medicine 1 academician of Mongolian Academy of Science, 4 Doctors of Science, and 28 Philosophy Doctors and many researchers and traditional medical doctors were born. Within historical study was done the review of traditional medical ancient treasures (Ts.Khaidav, 1975) and was developed methodology aspects of Mongolian and Tibetan traditional medicine (B.Boldsaikhan) and historical background and Buddhist philosophical complex property (S.Seesregdorj, 2002). New concept of Membrane Structure of Three elements theory (M.Ambaga, 1990) and the foundation of the theory and methodology of Mongolian and Tibetan medicine (B.Dagvatseren, 1995) founded modern scientific interpretation of Traditional medicine. Beside of fundamental and historical research scientific explanations of diagnosis (N.Tumurbaatar, 1998, Sh.Bold, 1998), therapy (scientific explanations of blood letting treatment by Pr. D.Tserendagva, 2000) and prevention were done. Dr. B.Boldsaikhan developed a program supply of diagnostic expert system in 1996.

Introduction: There are over 1500 plants on our planet that have anti-diabetes properties. Research findings suggest that more than 400 plant species showing hypoglycemic activity on experimental diabetes in animals.Healthy diet, regular physical activity, maintaining a normal body weight and avoiding tobacco use can prevent or delay the onset diabetes. Recently, numbers of high level researches were conducted worldwide to study the nature and mechanism to treat diabetes, tens of methods were discovered, and dozens of medical herbs were studies, yet very few herbal hypoglycemic drugs without side effects and at low cost are found. Scientists are still in search for development of new and better oral drugs for diabetes without side effect at relatively low cost. Materials and Methods: The research was conducted at the Scientific Research Center of “Monos” Institute of Traditional Medicine and in biochemical Laboratory of “Khuljborjigon” Clinic. For the experiment, we used 23 perfectly healthy mice of same sex and size which meets standards of laboratory testing. The Prozorovski3 quick method for the determination of LD50 in the water (20%) and ethanol extractions (30%) of Antidiabetes-3 preparation (AD3). The tested animals were the white mice. Following Erne (1963), Kovalev I.E.,(1976), Petrov’s (1980) 4 methodology of studying effects on immune system, we have Antidiabetes-3 preparation (AD3) were given to the 33 mice 2 times a day in 3ml/200gr dose, during 7 days. On third day of the experiment, we injected into vein 2ml of 10 % sheep’s RBC to stimulate the immunity. On the fifth day, we defined weight of pancreas, number of pancreatic cells, pancreatic index, and haemagglutination titre to screen RBC antibodies.Results: The method developed by V.B. Prozorovski for the calculation of average lethal number was used on 40 white mice (18-22g). Water extraction (10%) was per fused in the tail vein of the experience mouse and the lethal dose (LD50) was 88.9g/kg. These facts prove that the toxic effect of the AD is low. The water (10%) extractions of “Antidiabetes-3” (AD3) preparation were given to the mice 2 times a day in 3ml/200g dose, during 8 days. We have studying compared group “Salimon and Immunal mixture” (S&I) to the mice 2ml/200g dose, during 8 days. On third day of the experiment, we injected into vein 2ml of 10 % sheep’s RBC to stimulate the immunity. On the fifth day, we defined weight of pancreas, number of pancreatic cells, pancreatic index, and hemagglutinin to screen RBC antibodies (Table 1). Figure 1 demonstrates increase in mice’s spleen weight on the 5th day after stimulation of immunity with sheep’s RBC antigen. Spleen weight increase in AD3 group was 1.6 times higher compare to control group (AD3 group 0.16±0.08; control group 0.10±0.02; p<0, 05), and AD3 group was 1.0 times level compare to control group (AD3 group 0.16±0.08; S&I group 0.17±0.09; p<0, 05). In figure 2, the spleen index in control group was 1.24 times higher than in normal group (control group 0, 0047±0.001; normal group 0.0038±0.0004; p<0, 3), AD3 group’s index was 1.3 times higher compare to control group (AD3 group 0.0061±0.002, control group 0, 0047±0.001; p< 0.05), and 1.0 times lower compare to S&I group (AD3 group 0.0061±0.002; S&I group 0.0062±0.003; p< 0.05). In figure 3, the number of spleen cells of control group’s was 142.71±55.51*106/ml. this is 1.2 times lower compare to normal group which is 172.67±135.5 *106/ml. AD3 group’s spleen cell number was 329.78±187.78*106/ml and 1.61 times bigger than in control group. In comparison to control group, haemagglutination titre of AD3 group was 1.13 times higher (AD3 group 54.86±19.95%; control group 50±8.83%, p<0,05) and this indicates that BV has immunity stimulating effect.Conclusions:1. Was defined the Antidiabet-3 preparation LD50, 88,9g/kg, its toxicity of classification (Sydorov K.K 1973) was little toxicity.2. Was defined to immunity stimulating effect the Antidiabet-3 preparation

BackgroundTraditional Mongolian Medicine has a history of over 5000 years. Scientific development of TM hasstarted in 1959. Since 1999 Mongolia was categorized by WHO as a country having an Integrativesystem of TM- officially recognized and incorporated into all areas of health care provision, TMMresearch has been following key objectives of National R&D programs.AimIn order to assess the situation of TMM development we have conducted this study based on last10 years’ research done.Ìaterial and MethodsDocument study- we have selected key TMM’s R&D project implementers’ archive and humanresources documents.Descriptive and Analytic methods- a survey of 32 questions evaluating participation of TMMprofessionals in R&D work were conducted. Also, to clarify the point of view about TMM’s R&D6 focus group meetings with different level participants, such as professional committee, policymakers and research workers as well as health care providers, were organized.ResultsFrom 2004-2013, there are 28 projects implemented on TMM, 43% accomplished by TMMRTC,32.8% of which is resulting in raw materials standardization and technology study, related clinicalstudies standing 20% out of all studies done on TMM matter. These numbers are confirmed bysurvey and focus group interviews, more than 50% of participants willing to conduct a clinical studyand expressing difficulties such as lack of knowledge of methodology, policy support and revenue.Conclusions:1. TMM R&D has a potential growth due to human resources capacity. Practitioners are leastinvolved in R&D, due to lack of knowledge of methodology and revenue.2. There were 28 projects implemented on TMM matter, most of these are basic studies, fewerclinical studies done, resulting in pharmacopeia monographs and technological guidelines.

Over the last period in the field of theoretical and pathological pharmacology it has been quite typical to do different studies on the traditional medical preparations with the highly sensible methodology at tissue and cell levels in conformity with the accurate mechanism of the modern prescriptions with similar effects and to establish the reasons of using such preparations applied in traditional medical practice in combination with the contemporary, or replace them with the appropriate ones. However, the main difficulty in doing such research is that the full explanation or prediction about specific pharmacological effects of a certain number of preparations used in traditional medicine for the diseases coded with wind, bile, and rlung is limited in term of theory, in a way of explaining them at the cell levels. A type of such interesting diseases is Yam. The problem is that the notion and theory of traditional medicine as related to this disease, as well as the mechanism of preparations like rinchinnida are have not been studied yet or explained.

Goal: Research impact of “Sharkh - 2” preparation on pathological model of burning wound which was formed on experimental animal. Material and methods of the research: The experiment and research was made on pathological model of burning wound of 45 rats of Vistar breed on the basis of lab and material base of Institute of Traditional Medicine of School of Medicine, University of Medical Science, University of Medical Science in Huhhot city in Inner Mongolian Autonomous Region, National Corporation of Technology and Production of Traditional Medicine and “Sharkh - 2” preparation was applied by thin layer once on wound. Result: Wound area was decreased by 9.5% in control group in the 14th day and by 38.5% n the 28th day. In comparing group of “Sharkh - 2” with control group, there was not invisible and considerable result in the seventh day of the exerpeiment. But in “Sharkh-2” preparation, wound area decreased by 7.7% in the 14th day and by 62.9% in the 28th day. In comparison with control group, leukocyte of animals which used “Sharkh -2” preparation is less by 12.1% in the 7th day of the experiment, by 28.1% in the 14th day, 38.2% in the 28th day, sedimentation speed of red cell is less by 8.2% in the 7th day, by 12.3% in the 14th day and by 31% in the 28th day and TNF- α is not considerable I the 7th day of the experiment, 10.4% in the 14th day and by 13% in the 28th day. Conclusion: “Sharkh - 2” preparation which was extracted from raw materials of traditional medicine as Pulsatilla flavescens and Rosa aciccularis has impact to improve cure of burning wound of experimental animals.

Background: From the perspective of Mongolian medicine, hemorrhagic disease is a symptom of bleeding from any part of the body. This disease was compared to the immune thrombocytopenia disease of modern medicine. The treatment of this disease using two medical methods and the prevention of complications and relapses are issues facing the healthcare sector. In this regard, we have chosen this topic to clarify and prove the mechanism of action of the Mongolian drug Gurgem-8, which is widely used to treat bleeding disorders. Aim: To evaluate the therapeutic efficacy of Gurgem-8, in haemostatic treatment. Materials and Methods: The study was conducted using a randomized, controlled (active), open label, single centered clinical trial method. The study was conducted in two phases. First, an acute toxicity study of the Gurgem-8, was conducted in accordance with OECD guideline 423 and evaluated according to GHS classification. A chronic toxicity study was also conducted on Wistar rats (n=20) given the Gurgem-8, at doses of 500 mg/kg, 100 mg/kg, and 150 mg/kg daily for 60 days. Second, a clinical study was conducted on a total of 74 patients, who were randomly divided into 2 groups. The treatment group was given 3 grams of the Gurgem-8, daily, and the comparison group was given 4 capsules of Sheng Xue Xiao Ban Jiao Nang 3 times a day. The results were determined by laboratory methods. The study was conducted with the approval of the Research Ethics Committee of Mongolian National University od Medical Sciences (2024.01.19 №2024/3-01). Results: In the acute toxicity study, Turmeric-8 was found to be of low toxicity according to the GHS classification. No mortality was observed in the chronic toxicity test. As a result of the clinical study, there were significant differences in the blood hemoglobin (χ2=73.923, P<0.001), platelet (χ2=62.465, P<0.001), erythrocyte (χ2=77.113, P<0.001) and leukocyte (χ2=14.771, P<0.001) cell counts between the Gurgem-8, drug group and the comparison group. It was also determined that the platelet (χ2=138.3, P<0.001), erythrocyte (χ2=85.405, P<0.001) and leukocyte (χ2=10.961, P=0.027) cell counts were directly related to the treatment period and the group. When determining the effect on immune cells, there was no significant difference in the lymphocyte cell content before and after treatment (CD4+: t=0.233, P=0.817; CD8+: t=-0.264, P=0.793; CD4/CD8:Z=-0.119, P=0.905). However, the CD4/CD8 ratio was statistically significantly increased in each of the Gurgem-8, drug group and the comparison group (P<0.001, P=0.001). Conclusion In immune thrombocytopenia diseases, the Gurgem-8, has the effect of reducing hemoglobin levels in the blood, increasing platelet counts, reducing CD4+ and CD8+ T cell counts, and increasing the CD4/CD8 ratio.

Many plants have been used for the treatment of diabetes mellitus in traditional system of medicine and in other ancient systems of the world. Out of these only a few have been evaluated as per modern system of medicine. From many such plants only extracts have been prepared and their usefulness evaluated in experimental diabetes in animals. In some plants like extract Antidiabetes-3 (Cynarascolymus L,DasiphorafruticosaRydb. L,Tribulusterrestris) active hypoglycemic principles have been isolated and their mechanism of action studied. Most of them seem to act directly on in vitro assays to glucose uptake effects in normal and disiese human blood. Some have extra pancreatic effect also by acting directly on tissues like liver, muscle etc. and alter favourably the activities of the regulatory enzymes of glycolysis, gluconeogenesis and other pathways. Since the plant products have less side effects, they have the potential as good hypoglycemic drugs. They may also provide clues for the development of new and better oral drugs for diabetes. We have compared the in PBS of normal and disiese human blood, proves the glucose uptake effect of the Antidiabetes-3 preparation.

dicamentous therapy of liver disease there was a necessity to investigate herbal medicine possibility in traditional medicine. In Mongolian traditional medicine more than hundred prescriptions based on animal, mineral raw materials and more than 210 species of plants in various combinations used for liver remedy. But among them the greatest interest has caused us a plant named Chiazospermum erectum L., from the family of Hipecoaceae. In traditional medicine this plant is applied at liver infectious diseases as febrifugal, soothing, at some oncological diseases, diseases of a teeth, and mucous a pharynx. Aim of study was investigation of hepatoprotective effect of Chiazospermum erectum L. (dry extract) on experimental D-galactosamine hepatitis. Methods: Dry extract of Chiazospermum erectum L. was investigated on the experimental D-galactosamine hepatitis. It is well known D-galactosamine caused hepatitis demonstrated very similar pathogenesis and simptoms with viral hepatitis, especially with B-viral infection (Bluger A.F, Maiore A.Ya 1978). The experimental hepatitis was developed in Vistar bred laboratory rats (n=75) by intraperitoneal introduction of D galactosaminein a dose of 0.4 g/kg of animal weight. To animals of the first experimental group, since the beginning of experiment was intragastrically administered extract of Chiazospermum erectum L. in the form of water solution in a dose 25 mg/100gr of animal weights once per day within 14 days. To animals of other experimental group was administered a water solution of comparing (standard) preparation – Cholosasum into volume of 1.0 ml/100 g. In control group under the similar scheme introduced the distilled water in equivalent volume. The interval between administering of D galactosamine, standard preparation and distilled water was 5-6 hours. Results: The hepatoprotective activity of the dry extract of Chiazospermum erectum L. studied on ferment activity ALT, AST in experimental D-galactosamine hepatitis. The AST and ALT levels in the animals of experimental group was 20% and 46% lower than in indices of control group, and the levels of cholesterol, -lipoprotein and bilirubin were 18%, 12% and 14% lower, respectively. Chiazospermum erectum L. demonstrated a hepatoprotective activity on D-galactosamine hepatitis same with a standard remedy – Cholosasum. Conclusion: ThedryextractfromtheaerialpartofChiazospermum erectum L. during intragastric administering to laboratory animals with D- galactosamine liver damage reducing cytolytic syndromes and choleostasis, promoting the accelerated normalization of its functional condition. Chiazospermum erectum L. demonstrated a hepatoprotective activity on D-galactosamine hepatitis same with a standard remedy – Cholosasum. The received results demonstrated a further possibility of the studying of this plant for preventive maintenance and treatments of viral hepatitis and its complication