WHO informed that across world an average of 565 young people aged 10 to 29 die every day through interpersonal violence. Some studies mentioned that anxiety was most frequently occurred as one of the psychological consequences among victims of child abuse. Recent research on effects of adverse early life experiences on central nervous system as stress systems (hypothalamus-pituitary-adrenal axis-HPA) has provided a greater understanding of the link between childhood abuse and susceptibility to anxiety disorder. Therefore, this research was done to study anxiety among abused adolescents, some physical parameters and level of cortisol in saliva. There were selected 20 abused children and 40 non abused children aged between 11-16 years old and lived in Ulaanbaatar city. The Spence Children’s Anxiety Scale (SCAS; Spence, 1997) is a 38-item self-report questionnaire that assesses multiple symptoms of childhood anxiety disorders based on current diagnostic criteria. All participants were measured the cortisol in the saliva by Cortisol ELISA kit, Sigma, Average age of all subjects in the study was 13.52±1.57 and 59% of them were female and 41% were male. All anxiety symptoms of case group was statistically significance higher (p<0.01) than control group. The average amount of salivary cortisol of case group was (18.65±7.08) statistically significantly lower (p<0.01) than average amount of control group (32.15±20.99). Anxiety was more frequently occurred among abused children and blunted cortisol responses might indicate a level of impaired HPA functioning that could constitute a vulnerability to psychopathology with exposure to anxiety.

Introduction: There are over 1500 plants on our planet that have anti-diabetes properties. Research findings suggest that more than 400 plant species showing hypoglycemic activity on experimental diabetes in animals.Healthy diet, regular physical activity, maintaining a normal body weight and avoiding tobacco use can prevent or delay the onset diabetes. Recently, numbers of high level researches were conducted worldwide to study the nature and mechanism to treat diabetes, tens of methods were discovered, and dozens of medical herbs were studies, yet very few herbal hypoglycemic drugs without side effects and at low cost are found. Scientists are still in search for development of new and better oral drugs for diabetes without side effect at relatively low cost. Materials and Methods: The research was conducted at the Scientific Research Center of “Monos” Institute of Traditional Medicine and in biochemical Laboratory of “Khuljborjigon” Clinic. For the experiment, we used 23 perfectly healthy mice of same sex and size which meets standards of laboratory testing. The Prozorovski3 quick method for the determination of LD50 in the water (20%) and ethanol extractions (30%) of Antidiabetes-3 preparation (AD3). The tested animals were the white mice. Following Erne (1963), Kovalev I.E.,(1976), Petrov’s (1980) 4 methodology of studying effects on immune system, we have Antidiabetes-3 preparation (AD3) were given to the 33 mice 2 times a day in 3ml/200gr dose, during 7 days. On third day of the experiment, we injected into vein 2ml of 10 % sheep’s RBC to stimulate the immunity. On the fifth day, we defined weight of pancreas, number of pancreatic cells, pancreatic index, and haemagglutination titre to screen RBC antibodies.Results: The method developed by V.B. Prozorovski for the calculation of average lethal number was used on 40 white mice (18-22g). Water extraction (10%) was per fused in the tail vein of the experience mouse and the lethal dose (LD50) was 88.9g/kg. These facts prove that the toxic effect of the AD is low. The water (10%) extractions of “Antidiabetes-3” (AD3) preparation were given to the mice 2 times a day in 3ml/200g dose, during 8 days. We have studying compared group “Salimon and Immunal mixture” (S&I) to the mice 2ml/200g dose, during 8 days. On third day of the experiment, we injected into vein 2ml of 10 % sheep’s RBC to stimulate the immunity. On the fifth day, we defined weight of pancreas, number of pancreatic cells, pancreatic index, and hemagglutinin to screen RBC antibodies (Table 1). Figure 1 demonstrates increase in mice’s spleen weight on the 5th day after stimulation of immunity with sheep’s RBC antigen. Spleen weight increase in AD3 group was 1.6 times higher compare to control group (AD3 group 0.16±0.08; control group 0.10±0.02; p<0, 05), and AD3 group was 1.0 times level compare to control group (AD3 group 0.16±0.08; S&I group 0.17±0.09; p<0, 05). In figure 2, the spleen index in control group was 1.24 times higher than in normal group (control group 0, 0047±0.001; normal group 0.0038±0.0004; p<0, 3), AD3 group’s index was 1.3 times higher compare to control group (AD3 group 0.0061±0.002, control group 0, 0047±0.001; p< 0.05), and 1.0 times lower compare to S&I group (AD3 group 0.0061±0.002; S&I group 0.0062±0.003; p< 0.05). In figure 3, the number of spleen cells of control group’s was 142.71±55.51*106/ml. this is 1.2 times lower compare to normal group which is 172.67±135.5 *106/ml. AD3 group’s spleen cell number was 329.78±187.78*106/ml and 1.61 times bigger than in control group. In comparison to control group, haemagglutination titre of AD3 group was 1.13 times higher (AD3 group 54.86±19.95%; control group 50±8.83%, p<0,05) and this indicates that BV has immunity stimulating effect.Conclusions:1. Was defined the Antidiabet-3 preparation LD50, 88,9g/kg, its toxicity of classification (Sydorov K.K 1973) was little toxicity.2. Was defined to immunity stimulating effect the Antidiabet-3 preparation

WHO informed that across world an average of 565 young people aged 10 to 29 die every day through interpersonal violence. Some studies mentioned that anxiety was most frequently occurred as one of the psychological consequences among victims of child abuse. Recent research on effects of adverse early life experiences on central nervous system as stress systems (hypothalamus-pituitary-adrenal axis-HPA) has provided a greater understanding of the link between childhood abuse and susceptibility to anxiety disorder. Therefore, this research was done to study anxiety among abused adolescents, some physical parameters and level of cortisol in saliva.There were selected 20 abused children and 40 non abused children aged between 11-16 years old and lived in Ulaanbaatar city. The Spence Children’s Anxiety Scale (SCAS; Spence, 1997) is a 38-item self-report questionnaire that assesses multiple symptoms of childhood anxiety disorders based on current diagnostic criteria. All participants were measured the cortisol in the saliva by Cortisol ELISA kit, Sigma, Average age of all subjects in the study was 13.52±1.57 and 59% of them were female and 41% were male. All anxiety symptoms of case group was statistically significance higher (p<0.01) than control group. The average amount of salivary cortisol of case group was (18.65±7.08) statistically significantly lower (p<0.01) than average amount of control group (32.15±20.99).Anxiety was more frequently occurred among abused children and blunted cortisol responses might indicate a level of impaired HPA functioning that could constitute a vulnerability to psychopathology with exposure to anxiety.

BACKGROUND: Nowadays, in economy of Mongolia there are a lot of mining, construction, transportation and powerplant sectors with high risk, top rate of industrial accidents and occupational disease. Moreover, it’sbeen seen that noise, vibration and dust effect on worker’s health have a huge negative effects inthose sector’s workplaces.GOAL: Based on occupational condition assessment between 2010-2014 of Occupational condition andmonitoring department of Occupational Health Research Center, noise, vibration and general dustwere measured at workplace of mining, construction and power plant sectors.MATERIAL AND METHODS: Some issues of occupational hygiene were cross sectional studies. Based on archive of OHRC,information has been collected by fact methods. The results have been processed statistically onSPSS-17 statistic program and have been compared with relevant standards and normative.RESULTS: From 740 workplaces at 89 enterprises in 3 sectors, in 686 noises level has been measured. Theaverage level of noise measurements has been above the maximum allowed amount standards. Thehighest results were in sector of mining and construction.At total of 166 workplaces, the average vibration level was also above of maximum allowed amount.And also the highest results were seen in sector of mining.At total of 447 workplaces, the average general dust level was 3-5 times more than maximum allowedamount and the highest results were seen I sector of mining and construction.From some measurements of occupational hygiene at workplaces that attended in this study, generaldust amounts and vibration levels are straight low related and have a statistical importance.CONCLUTIONS:The amount of general vibration, noise and general dust in this research at sectors of construction,mining and power plant were higher than the standard of allowed amounts.

BackgroundTraditional Mongolian Medicine has a history of over 5000 years. Scientific development of TM hasstarted in 1959. Since 1999 Mongolia was categorized by WHO as a country having an Integrativesystem of TM- officially recognized and incorporated into all areas of health care provision, TMMresearch has been following key objectives of National R&D programs.AimIn order to assess the situation of TMM development we have conducted this study based on last10 years’ research done.Ìaterial and MethodsDocument study- we have selected key TMM’s R&D project implementers’ archive and humanresources documents.Descriptive and Analytic methods- a survey of 32 questions evaluating participation of TMMprofessionals in R&D work were conducted. Also, to clarify the point of view about TMM’s R&D6 focus group meetings with different level participants, such as professional committee, policymakers and research workers as well as health care providers, were organized.ResultsFrom 2004-2013, there are 28 projects implemented on TMM, 43% accomplished by TMMRTC,32.8% of which is resulting in raw materials standardization and technology study, related clinicalstudies standing 20% out of all studies done on TMM matter. These numbers are confirmed bysurvey and focus group interviews, more than 50% of participants willing to conduct a clinical studyand expressing difficulties such as lack of knowledge of methodology, policy support and revenue.Conclusions:1. TMM R&D has a potential growth due to human resources capacity. Practitioners are leastinvolved in R&D, due to lack of knowledge of methodology and revenue.2. There were 28 projects implemented on TMM matter, most of these are basic studies, fewerclinical studies done, resulting in pharmacopeia monographs and technological guidelines.

Mongolia has a long history of traditional medicine and it is greatly related with the nomadic culture that has been developed along with lifestyle, diet, and animal-husbandry works. Traditional Mongolian Medicine (TMM) is based on Buddhist philosophy, cosmo-energical teachings in 5 elements. It belongs to the Eastern medicine which originated from Indian and Tibetan traditional medicine. There is wide range of theory and conception source in Mongolian traditional medicine, which needs scientific explanation. Important branches of scientific study might be divided into following topics: theory (to gather information from old literature and study fundamental principles of traditional medicine), phytochemistry (strives for obtaining active principles from natural medicinal materials and standardize new preparations), pharmacology (to study pharmacological activity and mechanisms), exploration and cultivation of medicinal plants. Drug technology research activities have been intended mainly to produce new medicaments based on traditional recipes. During redevelopment period of traditional Mongolian medicine 1 academician of Mongolian Academy of Science, 4 Doctors of Science, and 28 Philosophy Doctors and many researchers and traditional medical doctors were born. Within historical study was done the review of traditional medical ancient treasures (Ts.Khaidav, 1975) and was developed methodology aspects of Mongolian and Tibetan traditional medicine (B.Boldsaikhan) and historical background and Buddhist philosophical complex property (S.Seesregdorj, 2002). New concept of Membrane Structure of Three elements theory (M.Ambaga, 1990) and the foundation of the theory and methodology of Mongolian and Tibetan medicine (B.Dagvatseren, 1995) founded modern scientific interpretation of Traditional medicine. Beside of fundamental and historical research scientific explanations of diagnosis (N.Tumurbaatar, 1998, Sh.Bold, 1998), therapy (scientific explanations of blood letting treatment by Pr. D.Tserendagva, 2000) and prevention were done. Dr. B.Boldsaikhan developed a program supply of diagnostic expert system in 1996.

In traditional Mongolian medicine, 18 liver disorders are counted and each of them is denominated by specific medical term. However, liver diseases described in traditional Mongolian medicine do not easily match liver diseases as specified by the western medical system. Therefore, it is important to detect active constituents in Mongolian plants which give a rational for an application in the conventional Western treatment of liver disease. Mongolian traditional medicine doesn’t indicate cancer diseases separately. Under the traditional medical term ‘liver disorder’ mentioned non-cancer and cancer diseases of liver. For this reason, medicinal plants and compounds are prescribed in common hepatobiliary ailments. The most important part of Mongolian traditional medicine is medication. Mongolian materia medica consists of plants, minerals, and animal parts or products, however, the plants have always made up the main part (about 80%) of the prescriptions. By the theory of TMM mostly young strength persons with “bile-fire” constitutions, who stayed enough time in warm dry conditions suffers from acute, “hot” quality liver disease, and there is a suitable to use a bitter tested and cool, blunt quality remedies. This principle of therapy explained what mentioned above quality plants might have an antioxidant activity; promote membrane stability and reduce cys-configurations of membrane. This interesting hypothesis was successfully studied on species of Gentiana acuta, Silibum marrianum, Salsola colina. During pharmacological study of Chiazospermum erecthum Bernh. species were identified main biological active substances such as protopine alkaloid. Within the studying of protopine alkaloid property species such as Chiazospermum erecthum Bernh Mongolian scientists interested in a bitter tested, cool, rough, blunt quality plants same with Chiazospermum, and identified high amount of protopine alkaloid in Berberis sibirica, Thalictrum, Chelidonum majus species. Further Chiazospermum erecthum L. was studied on D-galactosamine liver damage model, and determined that dry extract from aerial part of Chiazospermum erecthum L. reduced cytolytic syndromes and cholestasis, promoted the accelerated normalization of its functional condition. Lilium pumilum Delile flowers‘infusion is thought to have a protective effect on liver injures and improve the microvascular circulation disorder known as disseminated intravascular coagulation. Saussurea amara (L.) DC is widely spread medicinal plant in Mongolia and successfully used in Mongolian traditional medicine as a holeretic component. The content of biological active of compounds was examined with thin layer chromatography and apigenin was isolated and identified for the first time. Beneficial effects, such as dose dependent choleretic effects were seen with apigenin from aerial part of Saussurea amara (L.) DC in an isolated rat perfused liver model. Beside of single plant species hepatoprotective compounds were studied by Mongolian scientists. Prof. L.Myagmar (1974) determined that total amount of flavonoid from Lomatogonum caranthiacum wulfen at Br. and Achillea asiatica L.species increaced bile secretion of experimental animals and demonstrated hepatoprotective activity. “Barbad-10” is potent hepatoprotective compound. Traditional compound “Degd-3” used as a remedy for bloodletting preparation influenced on immunosuppressive cells positive location. Also were determined antioxidant and choleretic activity of total flavonoids from Bupleurum scorzonerifoilium, hepatoprotective activity of gammapyrone substances isolated from two species of Gentianaceae family: Gentiana acuta and Lomotogonium rotatum L. 11 types of flavon substances were isolated from Saxifraga hirculus L. and Lomatogonium carinthiacum Wulfen et Br. species, and was determined hepatoprotective action of Saxifraga hirculus L. In other hand, Saxifraga hirculus L. increased the biliary secretion and excretion speed, protected from toxic detergent effect of bile acid, improved detoxification effect of the liver, protected from the necrotic cell lysis, showed antioxidant and membrane stabilizing activity and these actions were compared with the standard medicines such as Legalon and Cholosasum which in some cases, above mentioned effects were higher than these drugs. Hepatoprotective action of Saxifraga hirculus L. may have been related to its contents such as compound flavonoids of the quercetin group, aglycone and bitter hyperoside. From traditional recipes of liver remedy were developed following new formulations which demonstrated hepatoprotective actions and influenced on bile secretion: “Hepamon, “Silodin”, “Saxifragen”, and “Berchessazin”.

Over the last period in the field of theoretical and pathological pharmacology it has been quite typical to do different studies on the traditional medical preparations with the highly sensible methodology at tissue and cell levels in conformity with the accurate mechanism of the modern prescriptions with similar effects and to establish the reasons of using such preparations applied in traditional medical practice in combination with the contemporary, or replace them with the appropriate ones. However, the main difficulty in doing such research is that the full explanation or prediction about specific pharmacological effects of a certain number of preparations used in traditional medicine for the diseases coded with wind, bile, and rlung is limited in term of theory, in a way of explaining them at the cell levels. A type of such interesting diseases is Yam. The problem is that the notion and theory of traditional medicine as related to this disease, as well as the mechanism of preparations like rinchinnida are have not been studied yet or explained.

Goal: Research impact of “Sharkh - 2” preparation on pathological model of burning wound which was formed on experimental animal. Material and methods of the research: The experiment and research was made on pathological model of burning wound of 45 rats of Vistar breed on the basis of lab and material base of Institute of Traditional Medicine of School of Medicine, University of Medical Science, University of Medical Science in Huhhot city in Inner Mongolian Autonomous Region, National Corporation of Technology and Production of Traditional Medicine and “Sharkh - 2” preparation was applied by thin layer once on wound. Result: Wound area was decreased by 9.5% in control group in the 14th day and by 38.5% n the 28th day. In comparing group of “Sharkh - 2” with control group, there was not invisible and considerable result in the seventh day of the exerpeiment. But in “Sharkh-2” preparation, wound area decreased by 7.7% in the 14th day and by 62.9% in the 28th day. In comparison with control group, leukocyte of animals which used “Sharkh -2” preparation is less by 12.1% in the 7th day of the experiment, by 28.1% in the 14th day, 38.2% in the 28th day, sedimentation speed of red cell is less by 8.2% in the 7th day, by 12.3% in the 14th day and by 31% in the 28th day and TNF- α is not considerable I the 7th day of the experiment, 10.4% in the 14th day and by 13% in the 28th day. Conclusion: “Sharkh - 2” preparation which was extracted from raw materials of traditional medicine as Pulsatilla flavescens and Rosa aciccularis has impact to improve cure of burning wound of experimental animals.