Lipolytic activity of adipose tissue with age has been widely investigated by many authors. There are differences on the conception of intrinsic lipolytic activity of a cell changed with aging. Since lipolytic activity has been expressed by amount of FFA release per wet weight, per triglyceride or per protein, increase of triglyceride or wet weight seems to be a main factor to affect on it. It is of interest, therefore, to investigate the intrinsic lipolytic activity of adipose tissue with aging from the relationship among FFA release, wet weight, protein and triglyceride content in adipose tissue.
In the present paper, we investigated effect of aging on lipolysis by using epididymal adipose tissue in 29, 35, 43, 57, 105 and 350 days rats. Rats were anesthetized by intraperitoneal injection of pentobarbital sodium (4mg/100g body weight) and after that epididymal adipose tissue were removed and washed out the blood by 25°C Krebs-Ringer solution. The fat pads were placed in glass bottles containing Krebs-Ringer bicarbonate buffer solution (pH 7.2-7.4), bovine serum albumin (4°C/ w/v) and epinephrine (3.9 pM/ litre) . Incubations were carried out in a shaking water bath for 120 min at 37°C under an atmosphere of 95% O₂-5% CO₂. Rate of lipolysis were determined by assaying the production of free fatty acids. Free fatty acids were titrated by Dole's method. Triglyc-eride and protein content in fat pads were assayd by Stern and Shapiro's method and Lowry's method, respectively.
The following results were obtained
1) Triglyceride-to-wet weight ratio and triglyceride-to-protein ratio in the adipose tissue corresponded to age (days) remarkably increased between 35 days and 43 days rats. However, after that, triglyceride-to-wet weight ratio was unchanged up to 350 clays rats.
2) Maximum lipolytic activity of adipose tissue in rats with aging was observed at 29 days in the range studied and after that its lipolytic activity decreased with aging. This decreased phase of lipolytic activity seemed to be divided into two phases : one was fast decrease phase which was observed in the rats before 43 days, and the other was slow decrease in the rats after 57 days.
3) Relationship between lipolytic activity and triglyceride-to-protein ratio in adipose tissue during aging showed inverse curvelinear relation, and it was found that lipolytic activity of adipose tissue was extremely inhibited at triglyceride-to-protein ratio more than ten.
From these results, we assumed that increase of triglyceride content in adipose tissue was related with the decrease of lipolytic activity during aging and that the lipolytic activity of adipose tissue would be enhanced with decrease in triglyceride-to-protein ratio inversely.

34 male rats (13 weeks old) were used in this experiment. 11 rats were at rest and 23 rats were loaded to run on a treadmill with 2 degree slope at a speed of 25m/min. Relation between quantities of blood substances (plasma glycerol, blood lactate and plasma free fatty acids ; FFA) and lipolytic activity of epididymal adipose tissue was studied to clarify a mechanism of lipolysis of the adipose tissue in the exercise.
1) Plasma FFA and glycerol began to increase in parallel at 30 min after the run, but at exhaustion only plasma glycerol enhanced.
2) Blood lactate increased at 10 min after the run and more at exhaustion.
3) Basal lipolytic activity of epididymal adipose tissue in vitro was not influenced by the run, while norepinephrine induced lipolysis of the adipose tissue decreased with lapse of running time. Relation between plasma FFA and lipolytic activity of epididymal adipose tissue was inverse during the run.
4) There was also inverse relation between blood lactate and lipolytic activity of adipose tissue induced with norepinephrine. From these results, it is assumed that the increase of plasma FFA during the exercise was not resulted from the increase of lipolytic activity of the adipose tissue stimulated with only norepinephrine and effects of other hormones (ACTH, Glucagon, Growth hormone and etc.) and their interactions on the lipolysis might be considered.

Triglyceride contents of liver, heart muscle and skeletal muscles (rectus femoris, tibiaris anterior, soleus, plantaris and extensir digitrum longus) were determined on 1, 3.5, 14, 18 and 21 months-old rats, and especially on 14 and 21 months-old rats composition of triglyceride fatty acids was analyzed and interrelation of these triglyceride contents with aging of rats was studied quantitively and qualitatively. Following results were obtained
1) Changs in liver and muscular triglyceride contents appeared on 14 months-old rats and were significant increase at 21 months-old rats.
2) Liver triglyceride content significantly decreased at 21 months-old rats (p<0.05) .
3) lrriglyceride contents of tibiaris anterior and extensor digitrum longus muscles were mostly unchangeable through the aging of rats.
4) But triglyceride contents of soleus, rectus femoris and plantaris muscles in 21 months-old rats significantly increased (p<0.05) and the individual difference was great.
5) In composition of triglyceride fatty acids, increase of C₁₈, C_18-1, C_18-2 and C_18-3 was observed in epididymal adipose tissue, soleus and rectus femoris muscle on 21 months-old rats, but in extensor digitrum longus and tibiaris anterior muscles.
From these results, changes of tissue triglyceride contents with aging of rats are different in each tissue and increase of triglyceride content is present with qualitative changes in composition of triglyceride fatty acids, increase of long chain and unsaturated fatty acids, and organic specificity, which is related to the functional changes with aging of rats is considered.

This study aimed to develop a new method of increasing water content in the cutaneous stratum corneum under a dry skin condition. For this purpose, the experiments were performed using 10 healthy women (age: 20 ± 5 years, height: 158 ± 4 cm, weight: 50 ± 6 kg) in winter to ensure the dry condition. The subjects immersed the right hand into a 42˚C bath for 10 min. Skin surface temperature and water content in the stratum corneum of the left forearm were simultaneously measured during and for 1 h after the hand warming. The skin surface temperature began to increase (P<0.05) 15 min after the hand warming and thereafter remained increased for 1 h. Similarly, the water content in the stratum corneum began to increase immediately after the hand warming and remained increased throughout the experiment. The present results suggest that warming of one hand is effective in enhancing skin moisture in the other forearm and thereby maintaining barrier function of the skin.

We have developed the new dynamometer to measure twist strength of upper limbs that was not investigated sufficiently before. This dynamometer can measure both twisting hold strength and torque at a time. Therefore, it can be applied to motion analysis at demonstrating muscle strength. Both development curve of the age group's average of the average hold strength of left and right hand { (LH⋅HS+RH⋅HS) /2} and the average twisting hold strength of left and right hand { (LF⋅HS+RF⋅HS) /2} showed the peak of development in man's thirties and in woman's twenties-thirties and then marked depreciation gradually with ageing. The average torque of forward twisting of left hand and that of right hand { (LF⋅T+RF⋅T) /2} showed the same tendency in respect of the peak of development too, but woman's curve showed the peak in twenties different from that in man and then didn't mark depreciation remarkably. From the analysis with respect to twisting motion by the subjects of right handedness, we have recognized that the twisting hold strength of right hand by the action of left or right forward twisting (LF·RH·HS, RF·RH·HS) is greater than that of left hand (LF·LH·HS, RF·LH·HS), the torque of forward twisting of left hand (LF·T) is greater than that of right hand (RF·T) and that the hand of back twisting supports the grip-torque dynamometer from the result of EMG. Therefore, we can understand that the stronger torque is generated by the motion of forward twisting of left hand which can support stronger than by that of right hand. But we have recognized too that the average hold strength and the average torque correlate to one another closely. Therefor, we can understand that it requires the stronger hold strength to generate the higher torque.

1) Children's data (10-18 years old) of back strength, height, grip strength and running long jump from data book of Japan Education Ministry (1964-1981) were analyzed. Back strength was mainly studied and was compared with other data (grip strength etc.) . The groups with high average values for back strength at 10-14 years old did not necessarily show high values for back strength at 17 years old, or vice versa. Values for back strength at 10-14 years old were not significantly correlated with those at 17 years old.
2) Using the data from data book mentioned above, tension of deep back muscle (FMUS) was calculated based on kinetic models (FMUS. I, II, III) . Calculated FMUS values were 3-5 times larger than measured back strength values at each age.
3) Relative change of back strength and FMUS in 1964-1981 were compared. All values for back strength were lower than those for FMUS except in 1967. During the late 1960th and the 1970th, average back strength values gradully decreased, but the decrease of FMUS was less obvious than that of back strength.
4) Based on the data of 422 children (7-12 years old, male and femele), values of diaphragm area were approximated with some assumptions, and then FMUS values were calculated. FMUS values calculated by approximate diaphragm area were significantly larger than those calculated by constant diaphragm area (465 cm2) .
The figure for getting FMUS values easily was offered to avoid troublesome calculation. This consists of two graphs, and one can read FMUS values with reasonable precision. Parameters needed for getting FMUS values are sexuality, height, body weight and back strength.

Introduction: We investigated the improvement in and the priorities of patient education training in community pharmacy from the student’s point of view to improve long-term practical training.
Method: We conducted a questionnaire survey to assess student satisfaction and the acquisition of behavioral objectives and analyzed the results with customer satisfaction analysis. Questionnaires were distributed to 32 students who had taken part in long-term practical training in the first year.
Results: Customer satisfaction analysis showed that 4 factors-“the frequency of patient education,” “the period of patient education,” “the aggressiveness of the student,” and “the facilitation by pharmacists on question-asking by the student”-were preferentially improved. In addition, we found that the level of acquisition varied according to the specific behavioral objectives.
Discussion: These results suggest that universities and community pharmacies must work together to organize the training system to increase the frequency of patient education and to improve students’ attitudes about patient education in community pharmacy.

We successfully performed one-stage definitive repair for 3 infants weighing 4.2, 6.1 and 5.2kg with complex coarctation without homologous blood transfusion. The priming volume of the bypass circuits was 195ml, and their lower hematocrit values during cardiopulmonary bypass were 15, 16 and 13%, respectively. In order to diminish the aortic cross clamp time, the aortic arch was repaired with the heart beating, using isolated cerebral and myocardial perfusion methods. The base excess in each patient decreased to -9.4, -8.0 and -4.9mEq/l during the rewarming phase, however, their postoperative hemodynamic and respiratory conditions were satisfactory. They have grown without any sequelae for at least 2 months.

　　From 2008 to 2014, we experienced 40 respiratory failure cases which required long-term ventilation in the post-ICU in Kakeyu Hospital. They had been referred to our hospital from acute care hospitals and most of them had been transferred on our regional medical cooperation system. Initially, we used long-term care beds for them, but as the number of serious cases increased, we moved them to acute care beds. As regards main causes of respiratory failure, post cardiac arrest syndrome topped the list with 12 cases, followed by chronic obstructive pulmonary disease, intractable neurological diseases and cervical cord injury. All these cases combined, the number came to 30 cases, accounting for 75% of all. The shortest stay in hospital was made by a patient with brain stem lymphoma. It was only 12 days, but the longest was made by a patient with post meningoencephalitis 6 years. Our care consisted not only ventilation and medical care but also giving a bath, walking with a type of wheelchair, and sunbathing at the rooftop of our hospital. In May 2014, we renovated the post-ICU from an acute care division to a physical disability patient ward. As the elderly population will increase, it is expected that the need for acute care will augment in parallel with an increase to the number of post cardiac arrest syndrome and the demand of long-term ventilation. Therefore, the community care system will need the post-ICU for cooperation with acute care hospitals.