Object To study the chemical constituents in Bidens bipinnata L Methods Isolation and purifica tion were carried out on silica gel, ODS column and HPLC; the chemical constitue nts were identified by physico-chemical properties and structurally elucidated by spectral analysis Results From EtOAc extract of B bipinnata, eight compounds were obtained and identified as: salicyl i c acid (Ⅰ), 9, 12, 13-trihydroxy-10, 15-octadecadienoic acid (Ⅱ), 9, 12, 13-trihydroxy-10-octadecaenoic acid (Ⅲ), benzyl O-?-D-glucopyranoside (Ⅳ), benzen ethyl O-?-D-glucopyranoside (Ⅴ), (Z)-3-hexenyl O-?-D-glucopyranoside (Ⅵ), eugenyl O-? -D-glucopyranoside (Ⅶ), 3-methyl-2-(2-pentenyl)-4-O-?-D-glucopyranosyl-△ 2-cyclopenten-1-one (Ⅷ) Conclusion All of these compounds, with the exception of Ⅰ, are obtained from the plant for the first time