Objective To discusse the expression and clinic opathologic significance of vascular endothelial growth factor(VEGF) and microvessel density(MVD) in primary liver cancer,an immunohistochemical staining.Methods The expressions of VEGF and Ⅷ factor were determined in 60 selected operation specimen from patients with primary liver carcinoma.Results It showed that the positive expression rate of VEGF in primary liver cancer was 63.3%,the MVD was (30.76?11.56).Among them,the MVD in positive VEGF expression was (46.37?15.85),and the MVD in negative expression was (18.97?8.68),there were great statistical difference between them(P0.05).Conclusion This study indicates that VEGF can facilitate the formation of microvessel of the tumor and has correlation with the growth,infiltration and metastasis of primary liver cancer.

Objective To study the relationship between intra-tumor micro vessel density (MVD) clinic opathological features and prognosis of patients with hapatocellular carcinoma(HCC). Methods 30 cases of pathological sections undergone radical surgery for HCC,were evaluated after HE staining and anti-CD 34 monoclonal antibody immuno-histochemical staining. The number of endothelia cells stained by anti-CD34 antibody was counted. The relationship between MVD and survival rate afte roperation was evaluated. Results The surviva Irate was significantly lower in the tumor of larger size, poor stage and higher MVD than that in the other group(P

Objective To investigate the degradation characteristics of the large-scale brain functional networks during aging by functional magnetic resonance imaging measurement and explore its intrinsic mechanism.Methods 40 healthy subjects including 20 elderly persons [mean aged(72.4 ±4.6)years] and 18 young persons [mean aged(23.9± 1.8) years] were enrolled in this study.All subjects underwent functional MRI scanning at blood oxygenation level-dependent contrast resting state.Four canonical resting-state networks,including the default mode network (DMN),dorsal attention network (DAN),executive control network (ECN),salience network,and visual network,were extracted by the seed zone and double regression methods.The functional connectivities in these canonical networks were compared between the young and elderly persons.Results Compared with young persons,the elderly showed the distinct and disruptive alterations in the large-scale aging-related resting brain networks.The impairment of ECN was the most serious,followed by the impairment of DAN.The salience networks and DMN showed relatively limited functional connectivity disruption.The networks associated to higher-order brain functions were impaired,while the visual network,which served as a network related to low-order brain functions,had no significant change.Conclusions The aged brain in healthy subjects is characterized by organized change in networks,and the selective impairments of large-scale brain networks were more significant in the networks associated to higher-order brain functions as compared with the networks related to low-order brain functions.

The clinical characteristics and radiological data of 6 cases of sacral agenesis in one single family were analyzed and a literature review was performed.On magnetic resonance imaging (MRI),all of them presented with a partial absence of sacral vertebra,including associations with lumbar abnormalities (n =2) and sacral agenesis (n =2).One case presented with fourth/fifth lumbar vertebra bone fusion and fifth lumbar/first sacral vertebra bone fusion.On radiology,4 cases had concurrent scoliosis.None of them had tethered cord,diastematomyelia or meningocele.The understanding of sacral agenesis may be improved after reviewing and summarizing clinical features and radiological findings.

Objective To evaluate the value of low-dose spectral CT imaging combined with ASIR recon-struction in differenting peripheral lung cancer from inflammatory mass. Methods We retrospectively analyzed the imaging data of 147 cases with 165 pulmonary nodules or masses underwent enhanced CT scans with spectral imag-ing mode from June 2015 to May 2016. Spectral curves,normalized slope rates,iodine-water concentration were measured on the lesions in arteral phase images. The differences of the spectral characteristic parameters were eval-uated statistically by indepengdent samples t test. Results Among 165 pulmonary nodules or masses,including 68 inflammatory mass and 97 peripheral lung cancer. In the arterial phase,the iodine concentration,water concentra-tion and normalized spectral curves rates of peripheral lung cancer were 10.93 ± 5.12,1033.96 ± 9.74,0.998 ± 0.66,which was obviously higher than those in inflammatory mass in 5.29 ± 0.96,1028.85 ± 9.31,0.620 ± 0.16. There were significant difference between peripheral lung cancer and inflammatory mass(0.000 ,0.008 ,0.001 in P values). Conclusion The iodine concentration,water concentration and normalized spectral curves rates has high value for differential diagnosis of peripheral pung cancer and pneumonia mass in the arterial phase using low-dose spectral CT imaging combined with ASIR reconstruction.

Objective To establish a mouse model of acute liver failure induced by lipopolysaccharide /D-galac-tosamine ( LPS/D-GalN) .Methods The optimum dose of LPS/D-GalN was determined by i .p.injection of eight differ-ent doses of LPS and D-GalN into 40 female C57BL/6 mice and observation of their survival time .Then, 32 female C57BL/6 mice were i.p.injected with the optimal dose of LPS/D-GalN and sacrificed at 0, 1, 4, 8 hours after the injec-tion, 8 mice in each group.The control mice received saline injection .Hepatic changes were observed by pathology and se-rum ALT, IL-6, MCP-1 and TNF-αwere measured by biochemistry or flow cytometry .Results LPS (2.5 mg/kg) and D-GalN (0.3 g/kg) were determined as the optimal dose for the establishment of mouse model of acute liver injury .Com-pared with the control group , the hepatocellular damages were progressing in a positive correlation with the time course after LPS/D-GalN administration .The level of serum ALT was significantly increased after LPS/D-GalN administration ( P <0.001).The levels of inflammatory cytokines IL-6, MCP-1 and TNF-αwere increased and reached a peak at one hour after LPS/D-GalN administration and then decreased almost to that of the control group 8 hours later(P<0.001).Conclusions The mouse model of acute liver injury is successfully established by LPS /D-GalN administration , and provide an effective animal model for the study of pathogenic mechanisms of acute liver failure and evaluation of therapeutic drugs .

Objective To investigate the clinical and laboratorial characteristics of patients with myelodysplastic syndrome ( MDS) and erythroid hyperplasia.Methods MDS patients whose bone marrow was hypercellular with erythroid lineage more than 50% and blasts account for less than 20% of non-erythroid cells were enrolled in this study.The ratio of mature erythrocytes to nucleated erythrocytes was no more than 20, namely MDS patients with erythroid hyperplasia ( MDS-E ).The retrospective analysis comprised 102 patients with MDS-E from the First Affiliated Hospital of Suzhou University.Clinical characteristics , karyotype , and the prognostic significance of erythroid hyperplasia were evaluated.Results A total of 48 MDS-E patients (47.1%) presented a variety of cytogenetic abnormalities.The most frequently involved chromosomes were chromosome 8 (39.5%of all abnormal karyotypes ), chromosome 7 (22.9%), followed by chromosome 5 ( 18.8%) , chromosome 1 ( 16.7%) and chromosome 20 ( 16.7%) .Hemoglobin ( Hb) level affected the prognosis by survival analysis.The overall survival ( OS) of MDS-E patients with Hb equal or more than 70 g/L was longer than that of patients less than 70 g/L ( P<0.001).Allogeneic hematopoietic stem cell transplantation (HSCT) significantly improved the OS compared with best supportive care (P<0.001) and chemotherapy (P<0.001).The extent of erythroid hyperplasia in bone marrow did not impact on prognosis ( P=0.187 ).Conclusions Compared with previous reports of MDS patients, MDS-E patients have higher level of erythroid hyperplasia , more common erythroid dyshematopoiesis , more frequent 8 and 1 chromosome abnormalities .The degree of erythroid hyperplasia is not correlated with prognosis.Allogeneic hematopoietic stem cell transplantation improves the prognosis.

BACKGROUNDTo investigate the prognostic factors of 355 patients with advanced non-small cell lung cancer for researching an individual treatment module.

METHODSFrom March 1988 to October 2000, after diagnosed by histology or cytology and staged by staging examinations, 355 novel advanced NSCLC patients (stage III, 134; stage IV, 221) were enrolled, who were given at least 2-cycle chemotherapy. Response rate and survival were observed and prognostic factors were analysed.

RESULTSOut of 355 patients, 101 got partial response and 147 had stable disease. Response rate was 28.45%, and tumor control rate was 69.86%. Median survival duration was 16 months. The 1-, 2-, 3- and 5-year survival rates were 58.41%, 29.35%, 14.60% and 8.60%, respectively. In the patients with stage IV, median survival duration was 14 months, and 1-, 2-, 3- and 5-year survival rates were 54.62%, 25.59%, 12.70% and 6.73%, respectively. COX multiple variable analysis showed that improved/stable ECOG score (P=0.044 0) and chemotherapy (after second line failure) combined with γ-IFN (P=0.039 0) had prognostic significance.

CONCLUSIONSImprovement of quality of life is quite important in the treatment of advanced NSCLC. Combination with γ-IFN or TAM and radiotherapy of primary tumor may be helpful to improve the survival of patients with stage IV.

Bone is a load-bearing organ in human body. Fatigue damage occurs readily at the modest loads to which bone is subjected during its habitual physiological usage. Even bone fracture may occur during vigorous activity. The nature of fatigue damage is that in bone there are very fine microcracks which are smaller than typical microcracks, and may occur at the level of hydroxyapatite crystals. But bone can repair microdamage by bone remodeling. Osteocytes play an important role of signaling during bone remodeling. Some researchers attempted to describe the process of bone fatigue damage and repair by mathematic, mechanical models in order to understand it well and to apply it well in clinical practice.
Animals ; Bone Remodeling ; physiology ; Bone Resorption ; pathology ; physiopathology ; Bone and Bones ; cytology ; injuries ; physiology ; Fractures, Stress ; pathology ; physiopathology ; Humans ; Models, Biological ; Stress, Mechanical

OBJECTIVETo detect hFgl2 expression in peripheral blood mononuclear cells in patients with chronic hepatitis B and liver cancer and explore its association with the severity of chronic hepatitis B.

METHODSThe protein expression of hFgl2 in peripheral blood mononuclear cells was detected in 78 patients with chronic hepatitis B (including mild, moderate, or severe cases), chronic severe hepatitis, or liver cancer, with 20 healthy volunteers as controls. The data were analyzed in comparison with the patients' alanine aminotransferase (ALT), aspartate aminotransferase (AST) and total bilirubin (TBiL and) levels.

RESULTShFgl2 protein expression was significantly higher in patients with chronic severe hepatitis and liver cancer than in the healthy volunteers and patients with chronic hepatitis B. The patients with chronic severe hepatitis had significantly higher hFgl2 protein expression than patients with liver cancer. In severe cases of chronic hepatitis B, hFgl2 protein expression was positively correlated with ALT, AST and TBiL, but these correlations were not found in mild or moderate cases.

CONCLUSIONSPeripheral blood mononuclear cells express hFgl2 protein, whose expression level increases with the severity of chronic hepatitis B.

Case-Control Studies ; Fibrinogen ; metabolism ; Hepatitis B, Chronic ; blood ; classification ; Humans ; Leukocytes, Mononuclear ; metabolism ; Liver Neoplasms ; blood ; classification