1.Effect of hypoxic precondition on apoptosis of hepatic ischemia reperfusion with the hepatectomy of transplanted hepatic cancer models in SD rants
Xiaofan HAN ; Peijian ZHANG ; Pengfei LI ; Jian WANG ; Tongmin XUE
International Journal of Surgery 2014;41(1):18-23,封3
Objective To investigate the effect of Heat shock protein 70 induced by hypoxic precondition on apoptosis of the remnant livers after the hepatectomy of transplanted hepatic cancer models in SD rants and it's possible mechanisms.Methods Sixty SD rats of transplanted hepatic cancer models were divided into group A (sham operation group),group B (IR + hepatectomy group),and group C (hypoxia precondition + IR + hepatectomy group),with 20 rats in each group.C group was given 10% oxygen atmosphere for 90 minutes before operation,In 15 minutes of hepatic vascular occlusion with pringle method,Left lateral of liver which tumor was located was resected.At 1,8,12,24 hours after operation,Five rats of each group were killed respectively,then the remnant liver tissues were sampled to measured the hepatocytes apoptosis rate with flow cytometry,expression of heat shock protein 70 (HSP70) and Bax were detected by Western blotting.The serum AST and ALT activities were measured by an automatic analyzer.The histological changes of the hepatocytes were also observed by optical microscopy.All the data were statistically analyzed.Results (1) Compared with A and B group,the HSP70 expression increased obviously in C group at each time point (all P < 0.05).The protein showed a weak expression in group A and B group,while the index had no significant differences between two groups (P > 0.05).(2) In B group compared with A group,the expression of Bax and the hepatocytes apoptosis rate were significantly increased (all P < 0.01 or P <0.05).However,amplitudes of the above changes in C group were significantly lower than those of B group (all P < 0.05).(3)The liver function was improved in group HP.The pathological examination showed that the liver tissues in A group were general normal and in C group were showed mild changes,but in B group presented evident pathological changes.Conclusions HP can protect against ischemia-reperfusion injury after the hepatectomy of transplanted hepatic cancer models in SD rants,one of possible mechanisms is that HP can induce HSP70 expression at great intensity,which may decrease expression of Bax protein and the apoptosis of hepatocytes.
2. A cross-sectional study of seroepidemiology of viral hepatitis among Uighurs in Shufu of Xinjiang
Zhoubin ZHANG ; Zhenxiang XUE ; Qiongying YANG ; Tongmin WANG ; Yaohui LI ; Chunying MA ; Xuelian SONG ; Gang WANG ; Dongmei LUO ; Mahat SULAYMAN ; Abdurahman RAYHANGUL ; Caihong ZHAO ; Yuzhong WANG ; Ming WANG
Chinese Journal of Preventive Medicine 2017;51(6):484-489
Objective:
To explore the status of seroepidemiology on hepatitis A, B and C among students and residents aged equal or greater than 18 years in south Xinjiang, and to provide scientific evidence for prevention and control of viral hepatitis.
Methods:
Uyghur students in four towns and villages were selected by cluster random sampling from Feb to May, 2015, and Uyghur residents aged 18 to 69 years were selected by stratified cluster sampling from May to September, 2016. 4 507 middle and primary Uygur students and 4 833 Uyghur resides equal or greater than 18 years attended this survey. Self-designed questionnaire was used to collect the demographic information. And Elisa test was adopted to detect HAV-IgG, HBsAg, HBsAb and HCV-IgG. Chi-square test was used to calculate the difference on antibody positive rate of three types of hepatitis among the participants.
Results:
The overall HAV-IgG positive rate was 99.45% (9 289/9 340). The positive rates were 99.70% (4 006/4 018) in male and 99.27% (5 283/5 322) in female (χ2=7.95,
3.Prevalence of diabetes mellitus and associated risk factors in Uygur population in Kashgar area of Xinjiang Uygur Autonomous Region
Zhoubin ZHANG ; Zhenxiang XUE ; Hui WANG ; Tongmin WANG ; Yaohui LI ; Xuelian SONG ; Xianfeng CHAO ; Gang WANG ; Xueji WU ; Nurmamat NAZIBAM ; Bawudun AYXAMGUL· ; Elyas GULBAHAR ; Ziyan ZHOU ; Baoshan SUN ; Yuzhong WANG ; Ming WANG
Chinese Journal of Epidemiology 2017;38(6):696-701
Objective To investigate the epidemiologic characteristics of diabetes mellitus (DM) in Uygur residents in Kashgar of Xinjiang.Methods The survey was conducted among the Uygur residents aged ≥ 18 years selected through stratified cluster sampling in Kashgar by means of questionnaire survey,physical examination and basic laboratory test.The prevalence of different groups were calculated and risk factors of DM was analyzed by logistic vegression model.Results A total of 4 608 adults were surveyed.The prevalence of DM was 11.31% (standardized prevalence:10.59%) and the prevalence was 13.65% (standardized prevalence:12.34%) in males and 10.04% (standardized prevalence:9.83%) in females.The prevalence increased with age.The prevalence of DM was higher than the prevalence of impaired fasting glucose (IFG) in people aged >60 years,especially in females.The rates of awareness,treatment and control of DM were 28.02%,21.31% and 5.57%,respectively.Multivariate logistic regression analysis indicated that people aged 45-55,55-65 and >65 years had higher risk of DM and the odds ratio were 2.08 (95%CI:1.24-3.48),2.73 (95%CI:1.63-4.56) and 3.90 (95% CI:2.24-6.78) for men and 2.63 (95% CI:1.71-4.02),3.14 (95% CI:2.00-4.94) and 5.56 (95%CI:3.47-8.92) for woman,respectively.Family history of DM (OR=2.88 for men,95%Cl:1.45-5.72;OR=2.52 for women,95%CI:1.49-4.26) and BMI≥28.0 kg/m2 (OR=1.77 for men,95%CI:1.19-2.64,OR.=1.80 for women,95%CI:1.30-2.50) were also risk factors for DM.Conclusion The prevalence of DM was high in Uygur residents in Kashgar,but the rate of awareness,treatment and control of DM were low.It is necessary to improve the detection rate of DM and conduct targeted prevention and control of DM.
4.Prevalence of dyslipidemia and normal blood lipids level in Uygur population in Kashgar area of Xinjiang Uygur Autonomous Region
Zhoubin ZHANG ; Zhenxiang XUE ; Xueji WU ; Tongmin WANG ; Yaohui LI ; Xuelian SONG ; Xianfeng CHAO ; Gang WANG ; Nurmamat NAZIBAM ; Bawudun AYXAMGUL ; Gulbahar ELYAS ; Ziyan ZHOU ; Baoshan SUN ; Yuzhong WANG ; Ming WANG
Chinese Journal of Epidemiology 2017;38(6):702-708
Objective To understand the prevalence of dyslipidemia and normal blood lipids level in Uygur diabetes patients in Kashgar prefecture in southern area of Xinjiang.Methods A total of 5 078 local residents aged ≥ 18 years (42.56% were men) selected through cluster random sampling in Kashgar were surveyed by means of questionnaire survey,physical examination and laboratory test,and 521 diabetes patients were screened.Results The overall prevalence of dyslipidemia in diabetes patients was 59.50% (310/521) with adjusted rate as 49.39%.Age ≥65 years,overweight,obesity and abdominal obesity increased the risk for dyslipidemia by 0.771 times (95% CI:1.015-3.088),1.132 times (95%CI:1.290-3.523),1.688 times (95%CI:1.573-4.592) and 0.801 times (95%CI:1.028-3.155) respectively.Compared with males,female was a protective factor for dyslipidemia (OR=0.507,95%CI:0.334-0.769).The overall normal rate of blood lipids level including total cholesterol (TC),triglycerides (TG),high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) for type 2 diabetes patients was 11.13%.Female,higher BMI and abdominal obesity were the factors influencing the overall normal blood lipids level.The normal rate of LDL-C level decreased with increase of age,BMI and waist circumferences (trend test x2=18.049,P< 0.001;trend testx2=10.582,P=0.001;x2=19.081,P<0.001),but increased with educational level (trend testx2=9.764,P=0.002).Conclusion The prevalence of dyslipidemia in Uygur diabetes patients in Kashgar was high,however,the overall normal rate of blood lipid level was relatively low.Obesity was the most important risk factor for dyslipidemia in this area.More attention should be paid to dyslipidemia prevention in women.
5.Prevalence of hypertension and risk factors in Uygur population in Kashgar area of Xinjiang Uygur Autonomous Region
Zhoubin ZHANG ; Zhenxiang XUE ; Haiyan CHEN ; Tongmin WANG ; Yaohui LI ; Xianfeng CHAO ; Gang WANG ; Dongmei LUO ; Xueji WU ; Nurmamat NAZIBAM ; Ayxamgul BAWUDUN ; Elyas GULBAHAR· ; Ziyan ZHOU ; Baoshan SUN ; Yuzhong WANG ; Ming WANG
Chinese Journal of Epidemiology 2017;38(6):709-714
Objective To investigate the prevalence of hypertension and risk factors in Uygur adults in Kashgar of Xinjiang.Methods A total of 4 748 Uygur adults aged ≥ 18 years selected through cluster random sampling in Shufu county of Kashgar prefecture were surveyed by means of questionnaire survey,physical examination and laboratory test.The prevalence,awareness,treatment and control rate of hypertension of different groups were calculated and risk factors of hypertension was analyzed by using non-conditional logistic regression model.Results The overall prevalence of hypertension was 15.73% (age-adjusted prevalence was 13.75%).The prevalence rates of hypertension in men and women were 16.36% (age-adjusted prevalence was 12.96%),15.39% (age-adjusted prevalence was 14.34%),respectively.The rates of awareness,treatment and control of hypertension were 59.57%,52.74%,21.29%,respectively.The prevalence of hypertension increased with age.Compared with age group 18-34 years,the age groups 55-64,≥65 years had higher risk of hypertension and the OR values were 10.53,20.96 for men and 16.27,33.20 for women.The overweight (OR=1.47 for men,OR =1.82 for women,P<0.05) and obesity (OR =1.88 for men,OR=2.66 for women,P<0.05) also increased the risk of hypertension.The groups with family history of hypertension (OR=3.85 for men,OR=2.34 for women,P<0.05) also had higher risk of hypertension.Hypertriglyceridemia was positively correlated with the prevalence of hypertension in men (OR=1.62,95%CI:1.09-2.41).Conclusions The prevalence of hypertension in Uygur adults in Kashgar area of Xinjiang was at relatively low level.The related risk factors were age,overweight,obesity,family history and hypertriglyceridemia in men and the risk factors were similar in women except hypertriglyceridemia.