Objective To investigate the clinical characteristics and treatments of early-stage acute pancreatitis following laparoscopic cholecystectomy (LC). Methods From April 1998 to June 2006,totally 18 470 cases of LC were performed in our hospital,16 (0.09%) of them developed early-stage acute pancreatitis (

OBJECTIVETo investigate the transcriptional regulation of pacemaker channel I(f) mediated by vasoactive peptide endothelin-1 (ET-1) in neonatal rat ventricular myocytes and its mechanism.

METHODSNeonatal rat ventricular myocytes were enzymatically isolated. I(f) current was recorded using the whole-cell patch-clamp technique. The expression of hyperpolarization-activated cyclic nucleotide-gated channel (HCN) isoforms HCN2 and HCN4 were measured by quantitative RT-PCR.

RESULTSET-1 increased the expression of HCN2 and HCN4 mRNA in a dose- and time-dependent manner. These effects were blocked by specific ETA receptor antagonist BQ-123 but not the ETB receptor antagonist BQ-788. The effects of ET-1 on HCN2 and HCN4 mRNA expression were not affected by the p38 mitogen-activated protein kinase (MAPK) inhibitor (SB-203580).

CONCLUSIONThese findings indicate that ET-1 stimulates the expression of pacemaker channel I(f) in cardiomyocytes via ETA receptor through a p38 MAPK-independent signaling pathway, which might be linked to the intrinsic arrhythmogenic potential of ET-1.

Animals ; Animals, Newborn ; Cyclic Nucleotide-Gated Cation Channels ; drug effects ; Endothelin-1 ; metabolism ; Imidazoles ; pharmacology ; Myocytes, Cardiac ; drug effects ; metabolism ; Oligopeptides ; pharmacology ; Patch-Clamp Techniques ; Piperidines ; pharmacology ; Pyridines ; pharmacology ; Rats ; Rats, Sprague-Dawley ; Signal Transduction ; drug effects ; p38 Mitogen-Activated Protein Kinases ; metabolism

Objective To investigate the effects of hederagenin (HDG) on proliferation, migration, invasion and apoptosis of glioblastoma (GBM) cells and involved mechanism.Methods Human GBM cell lines U87, U251 and human brain glial cell line (HEB) were selected as the study subjects, and HDG 0μmol/L (or 0 mg/kg) was used as the control group.MTT, EdU staining and cell plate cloning were used to detect the effect of HDG on the proliferation of GBM cells.Trypan blue staining was used to detect GBM cell death affected by HDG.The effects of HDG on migration and invasion of GBM cells were detected by cell scratch and Transwell assay.To analyze the effects of HDG on apoptosis of GBM cells, apoptosis-related proteins Bcl-2, Bax, p53 and cleaved caspase-3 were detected by Western blot.Mitochondrial potential change was detected by JC-10 staining, and apoptotic cell count was displayed by Annexin V-FITC staining.The effect of HDG on tumor bearing in GBM was analyzed by xeno-transplantation in BALB/C mice.Results Compared with the control group (HDG0 μmol/L), HDG significantly inhibited the proliferation, migration and invasion of U87 and U251 cells, and they were dependent on the use dose of HDG.Trypan blue staining showed that HDG obviously increased death number of GBM cells.The mitochondrial potential of GBM cells was remarkedly decreased, the number of apoptotic GBM cells obviously increased, the ex-pressions of apoptosis-related proteins p53, Bax, cleaved-caspase3 were up-regulated and Bcl-2 was down-regulated by HDG in U87 and U251 cells.HDG significantly inhibited the size of subcutaneous GBM, the Ki67 positive rate of GBM cells and caused a large number of GBM cells to die in BALB/C mice.HDG had no obvious toxic effect on human HEB cells and the liver of tumor-bearing mice.Conclusion HDG can significantly inhibit the prolifera-tion, migration and invasion of GBM cells and induce the apoptosis of them.The mechanism of HDG induced apop-tosis of GBM cells may be through mitochondrial damage and regulation of p53 and Bcl-2/Bax expression.

Objective:To apply the best evidence of hypoglycemia management for neonates delivered by mothers with gestational diabetes mellitus (GDM) in clinical practice and evaluate its curative effects.Methods:This study was a historical controlled study. The newborns delivered by GDM mothers who were admitted to the neonatal intensive care unit of a Class A hospital in Hefei from February to December 2019 were selected as the research objects by convenient sampling. Newborns delivered by GDM mothers admitted to the hospital during the application of evidence-based practice were the subjects of the pre-evidence study ( n=53) , and newborns delivered by GDM mothers admitted to the hospital after applying the evidence-based practice from September to December 2019 were used as evidence Post-application research subjects ( n=59) . Evidence-based nursing was used to obtain best evidence. Based on the evidence-based continuous quality improvement model as the theoretical framework, the evidence-based practice plan for neonatal hypoglycemia management of gestational diabetes mothers during delivery was constructed and applied. Before and after the application of evidence-based practice, 63 neonatal nurses and parents of neonates were surveyed with the self-made GDM Mothers' Newborn Hypoglycemia Management Questionnaire and the Chinese version of the Uncertainty of the Disease Parents Scale, respectively. And the changes in the incidence of hypoglycemia within 2 hours after birth was compared before and after the application of evidence-based practice. Results:A total of 7 pieces of evidence were used in the evidence-based practice. After the application of the evidence-based practice, the incidence of hypoglycemia in neonates delivered by mothers with gestational diabetes within 2 hours after birth decreased from 20.8% (11/53) to 3.4% (2/59) , and the difference was statistically significant (χ 2=8.206, P<0.05) . The score of the questionnaire on knowledge of hypoglycemia management of neonates delivered by mothers with gestational diabetes mellitus in 63 department nurses was increased from (7.00±1.43) to (7.84±1.18) , and the difference was statistically significant ( t=-12.253, P<0.05) . Before and after the application of evidence-based practice, scores of Mishel Uncertainty in Illness Scale for newborn parents were respectively 87.00 (65.00, 99.50) and 59.00 (43.00, 74.00) , and the difference was statistically significant ( U=728.500, P<0.01) . Conclusions:Evidence-based practice for blood glucose management of newborns delivered by gestational diabetic mothers can reduce the incidence of neonatal hypoglycemia and the uncertainty of their parents' diseases, improve nurses' neonatal hypoglycemia management level, and promote continuous improvement of nursing quality.

Objective: To study the diagnostic value of a multi-gene molecular testing in cytologically indeterminate thyroid nodules. Methods: From February 2018 to September 2018, patients with thyroid nodules who underwent fine needle aspiration(FNA) at Peking University Cancer Hospital were enrolled. Three hundred and sixty patients were included, consisting of 86 men and 274 women, with a mean age of 45.8 years (between 13 and 89 years old). Among 391 nodules, 141 were cytologically inderminate and 75 were resected. FNA samples underwent prospective testing using a next-generation sequencing (NGS) assay, which included 16 genes for point mutations and 26 types of gene fusions. The testing results of indeterminate nodules were compared with surgical outcomes, to determine the diagnostic performance. The results were compared with the BRAF V600E single gene mutation analysis by χ² test. Results: The multi-gene testing showed a sensitivity of 73.2%, specificity of 96.8%, positive predictive value of 96.8%, and negative predictive value of 73.2%. The diagnostic accuracy of multi-gene testing was significantly higher than the BRAF V600E mutation test (83.3% vs 73.6%, χ²=31.588, P<0.01). Conclusion Multi-gene testing in FNA samples is an effective method to diagnose cytologically indeterminate thyroid nodules, which has a higher accuracy than BRAF V600E mutation detection.