The protective effect of silybin on the micro-model of Coxsackie B5 virus infected new born rat heart cell culture was observed. After inoculation with 400TCID Coxsackie B5 virus, the cardiac enzyme s-lactic acid dehydrogenase (LDH) and glutamic oxaloacctic transaminase (GOT) were much lower in the silybin-treated groups than in those groups of untreated, but the synthesis rate of DNA was higher. Virus titer in the supernatant of cultures was detected 96 h after virus challenge. The virus titer (Lg 3. 95 TCID50) of the silybin-treated was lower than in that of untreated groups (Lg 6. 95 TCID50). These results suggest that silybin may be a useful drug in treatment of acute viral myocarditis.

Objective To investigate the expression levels of E cadherin (E cd) and proliferation related antigen Ki67 and their roles in the mechanism of invasion and metastasis of non small cell lung carcinoma(NSCLC). Methods The expression levels of E cd and Ki67 antigen were detected by immunohistochemistry. Results The positive rates of E cd in the cases of Ⅲ Ⅳ stage and Ⅰ Ⅱ stage were 33 3% and 65 7%, respectively, but those of Ki67 were 96 3% and 68 6%. The expression levels of E cd and Ki67 in cases with metastasis were significantly different from those in cases without metastasis. Conclusion The expression levels of E cd and Ki67 are closely associated with invasion and metastasis of NSCLC. The expression level of E cd is negatively correlated with that of Ki67 in NSCLC.

Taking Coxsackie B3 virus cDNA labelled by Biotin as detecting probes and 25 kinds of standard enteroviruses as detected viusr, a spot nucleic acid hybridization method has been established to detect enterovirus RNA gene. The method has simple operation and its sensitivity is 50-80 TCID50 spot. Reagent is safe and stable, and may be stored for long period. pCBHⅢ/35.51 probe used has wider hibridization spectrum to enteroviruses; pCBⅢ/29 only reacts with RNA of Coxsackie B3 virus in strict condition. Consequently, the hybridization character which these probes possess is suitable to determining enlerovirus in clinical samples.

The protective effect of Astragalus saponins or Astragalus mongholicus bunge on the micro -model of cultured new born rat heart cells infected with Coxsackie B, virus was observed. After inoculation with 1000 TCID Coxsackie B, virus, the cardiac enzyme lactic acid dehydrogenase (LDH) was much lower in the Astragalus saponins or Astragalus mongholiais bunge treated groups than in those untreated groups, but the synthesis rate of DNA was higher. Virus titer in the supernatant of cultures was detected 48 h after virus challenge. The virus titer of the Astragalus saponins (Lg 3.78 TCID) or the Astragalus mongholiais bunge (Lg 4.33 TCID50) treated groups was lower than in those untreated groups (Lg 5.78 TCID50). These results show that the Astragalus saponins may be a useful drug in treatment of acute viral myocarditis.

The present study investigated the effect of BN50739, a new PAF antagonist, on the early reperfusion-induced arrhythmia in anesthetized SD rats, and by this to elucidate the role of PAF in the mechanism of early reperfusion-induced arrhythmia. The results indicated that the BN50739 group had lower incidences of premature ventricular contraction (PVQ, ventricular tachycardia (VT) and ventricular fibrillation (VF) during reperfusion than the control group (58.3% vs 66.7%, 50% vs 77.8%, and 16.7% vs 88.9%). But only VF incidence had a significant difference. Also the BN50739 group had shorter duration of VT+VF and of total arrhythmias than control group (58.8+171.9 vs 296.4+291.4, and 76.4+173.9 vs 361.8 + 286.5, P

The level of interferon (IFN) in serum and homogenized cardiac tissue, and the activity of natural killer cells in Coxsackievirus B3 induced myocarditis in BALB/c mice were determined. NK cell cytolytic activity and IFN titers peaked on day 3~ 7 postinoculation (p.i.) and then declined. Virus titers in heart tissue reached a maximum on day 7 p.i. and then declined. These results suggest that the increase of NK cell activity and IFN titers provides some protection against Cox B, induced myocarditis by limiting virus replication in heart tissue.

Alopeeia areata (AA) has been well recognized with familial tendencies, but the genetic basis of this clinical observation remains unknown. The cytokine interleukin-1 receptor antagonist (ILlra) is a potent anti-inflammatory protein that can prevent immune-mediated inflammatory response in the skin. We characterized a polymorphism within the gene for this cytokine ILlra in this study and tested the gene as a possible marker in patients with alopecia areata. We have determined allele frequencies of the polymorphic cytokine genes in a control population and a group of 72 patients with alopecia areata. The frequency of allele 2 of interleukin-1 receptor antagonist in patients with AA was significantly higher than that of control group. It suggests that interleukin-1 receptor antagonist gene may be a candidate gene or severity factor for alopecia areata.

Objective To observe the role of programmed cell death 5(PDCD5) gene combined with cisplatin for inducing the ap-optosis of human lung adenocarcinoma A549 cells and to investigate its possible mechanism .Methods The PDCD5 recombinant plas-mid was transiently transfected into A549 cells by lipofectamine .Its transfection efficiency was detected by RT-PCR .The expressions of trasfected PDCD5 protein ,Bcl-2 and Survivin protein were examined by Western bolt .The apoptosis of A549 cells by single PDCD5 and its combination with cisplatin was measured by the MTT method and the flow cytometry .Results PDCD5 recombinant plasmid was transfect into A549 cells successfully .The Western blot results showed that the expression of PDCD5 protein in the transfection recombinant plasmid group was higher than that in the blank control group and the transfection empty plasmid group ,while the expres-sion of Bcl-2 and Survivin protein was lower than that in the other two groups ,the difference was statistically significant (P<0 .05) . The MTT and flow cytometry results demonstrated that the cell apoptosis rate in the transfection recombinant plasmid group was higher than that in the blank control group and the transfection empty plasmid group (P<0 .05) .Conclusion PDCD5 gene may pro-mote cisplatin induced A549 cells apoptosis .

lecithin. No dissolution of DEHP was noted in lecithin. In a certain kind of surfactant, the dissolution of DEHP was positively correlated with the concentration of surfactant and the infusion time and the length of the infusion tube. CONCLUSIONS: The results serve as reference for the choice of safe infusion set when surfactants is contained in the injection and the choice of surfactant in the production of injection in pharmaceutical facoties.

Objective:A combined therapy of Aidi and Kanglaite injection was employed to enhance the living quality of patients with tumors.Method: 50ml Aidi was added into 5% glucose 500ml,together with100 to 200 ml Kanglaite was injected per day,a medical treatment course lasts for 15 to 20 days,and the duration includes 1 to 3 courses.Result: 25% of all patients treated show a complete cure of tumor pain,43.8% of all patients show relief to some extent,with a total efficiency of 68.8%,and 72.8% of all treatment group show an increase in living quality.Conclusion: The combined therapy together with humanistic nursing care results in a significant effect in treating patients with terminal tumors.