Objective To establish HPLC method for fast, effective, simultaneous determination of Vancomycin and Norvancomycin in serum. Methods The separation was achieved on the Hypersil ODS2(250 mm ×4.6 mm,5μm)with methanol-acetonitrile-a mixture of potassium phosphate (9.5:2.5:88) as the mobile phase.The flow rate was 1.0 mL/min.The UV detecting wavelength was 280 nm.The temperature of the column was 35 ℃. Vancomycin and Norvancomycin were used as the internal standard for each other .Results Vancomycin and Norvancomycin blood concentration were in well linearity in the range of 1.25-100μg/mL,R2 =0.9996.The average method recovery rate of three concentrations were 96.88%,99.50%,105.01%respectively, the average method recovery rate of three concentrations were 94.40%,103.90,103.81%respectively, the intra-day and inter-day RSD were less than 5%.Conclusion The HPLC method is proved to be simple,sensitive, accurate and precise in which little blood sample is taken, thus it is suitable for the therapeutic drug monitoring(TDM) and pharmacokinetic study of Vancomycin and Norvancomycin.

Objective:To introduce the use of fluoxetine in neurologic disease,especially in epilepsy.Method:We used fluoxetine as a supplement antiepileptic drug in 25 patients who couldnt be controlled yet by routine antiepileptics. A long term follow-up with these cases was carried out.Results:Most patients got better outcome.Conclusion:Fluoxetine would be an important supplement antiepileptic drug and have value for further clinical and experimental research.

Humans ; Lung Neoplasms ; chemically induced ; Proportional Hazards Models ; Radon ; adverse effects

Objective To analyze the value of serum calcitonin level to determine the illness severity of the elderly patients with severe pneumonia within six hours after admission and to dynamically observe the predictive value of serum PCT level change rate in different time points on the prognosis of the patients.Methods A total of one hundred and twenty olderly patients with severe pneumonia were enrolled.Within six hours after admission,the serum PCT level was detected by enzyme linked fluorescence analysis.Acute physiology and chronic health score (APACHEⅡ)were used to assess the severity of the disease.The correlation between serum PCT and APACHEⅡ score was analyzed by Pearson correlation coefficient.The receiver operating characteristic curve (ROC curve)showed the value of serum PCT level for the prognosis.Serum PCT levels were respectively monitored on the 1st,2nd,3rd and 5th day. Based on the prognosis outcome on twenty -first day after admission,the patients were divided into survival group and death group.The changes of serum PCT level in two groups were compared and the rate of change of serum PCT was also compared and the value of the clinical mortality of twenty -first day was assessed.Results Within six hours after admission,the level of serum PCT was positively correlated with APACHE Ⅱ score (r =0.768,P <0.05).The receiver operating characteristic curve (ROC curve)showed that the level of serum PCT was low for the predictive value on the prognosis of the patients,and the area under the curve was 0.502(t =0.915,P =0.153).All patients, there were ninty -four cases in the survival group and twenty -six cases of death group.Dynamic observation of serum PCT levels in the survival group showed a trend of gradual decline with time prolong,the level of PCT change rate was more than or equal to 50 percent within 72 hours.The level of serum PCT change rate in death group showed a gradual upward or downward trend and the change rate of PCT in seventy -two hours was less than 50%.The ROC curve showed that the rate of change of serum PCT level in seventy -two hours was better in predicting the mortality of patients on the twenty -first day,and the area under the ROC curve was 0.892(t =3.895,P =0.001).Conclusion Dynamic monitoring of serum PCT levels can be helped to assessment the prognosis of patients with sepsis and also predict the severity of the illness.The level of serum calcitonin can reflect the severity of severe pneumonia in elderly patients within six hours after admission,but can not accurately determine the prognosis of patients with severe pneu-monia.Dynamic monitoring of changes in serum calcitonin level is helpful to judge the prognosis of the elderly patients with severe pneumonia and the predictive value of serum calcitonin level change rate is higher in seventy -two hours after admission.

Ubiquitin-specific proteases (USPs) regulate the deconjugation of ubiquitin tag from substrate proteins. Dysregulation of USPs has been linked to many diseases. Despite extensive efforts by academia and industry,high quality chemical molecules targeting the USPs family of ubiquitin enzymes are still scarce. In this commentary,I propose the criteria of a high quality chemical probe and the strategies to target USPs.

Breakthroughs in the immunotherapy of acute leukemia have been achieved in recent years. The paper summarizes the clinical results, experience, and prospect in this area. One of the most significant advancements is the finding that anti-CD19 chimeric anti-gen receptor T cells (CAR-T) or CD3/CD19 bispecific antibody increases the rate of complete remission in patients with refractory re-lapse acute B lymphocytic leukemia. The disease-free survival of patients with low or intermediate risk was dramatically improved by combining chemotherapy and adoptive cytokine-induced killer or natural killer cells. Many immunotherapy methods, such as those fo-cusing on other targets of CAR-T, T-cell antigen receptor-modified T cell, and other targets of bispecific antibodies, are currently being examined. Combined methods can further increase cure rate and improve patients' quality of life, decreasing application of allogeneic hematopoietic cell transplantation which increases risks and reduces the quality of life.

Objective To observe the clinical effect of Zhongfeng-Tengtong decoction combined with tramadol in the treatment of central post-stroke pain (CPSP). Methods A total of 121 patients with stroke were recruited in our hospital. The patients were randomly divided into 2 groups by the ramdom number table method, 61 patients in the treatment group and 60 in the control group. All patients received conventional treatment of stroke secondary prevention, and the control group received oral tramadol hydrochloride sustained release tablets, while the treatment group received Zhongfeng-Tengtong decoction based on the intervention of control group. Both groups were treated for 14 days. The outcomes included Numerical Rating Scale (NRS), Fugl-Meyer Assessment (FMA), Stroke Specific Quality of Life Scale (SS-QOL), and the changes of hemorheology indexes of the 2 groups before and after treatment. Results After treatment, the NRS scores of treatment group were significantly lower than the control group (2.5 ± 0.4 vs. 4.6 ± 0.7;t=20.303, P<0.01). and FMA and SS-QOL scores of treatment group were significantly higher than the control group (FMA 57.1 ± 18.1 vs. 44.2 ± 12.5;t=4.702, P<0.01), (SS-QOL 193.4 ± 25.6 vs. 165.4 ± 22.8;t=6.536, P<0.01). The whole blood viscosity, plasma viscosity, fibrinogen and thrombosis coefficient of treatment group were significantely lower than the control group (whole blood viscosity 4.22 ± 0.13 mPa?s vs. 4.56 ± 0.41 mPa?s, t=14.452, P<0.01;plasma viscosity 1.72 ± 0.31 mPa?s vs. 2.33 ± 0.23 mPa?s, t=14.258, P<0.01; fibrinogen 3.72 ± 0.49 g/L vs. 4.98 ± 0.41 g/L, t=17.783, P<0.01; thrombosis coefficient 0.76 ± 0.30 vs. 1.11 ± 0.20, t=8.178, P<0.01). Conclusions Zhongfeng-Tengtong decoction combined with tramadol could alleviate the pain symptom in patients with CPSP, improve the movement function of limbs and improve the quality of life.

Objective To observe the effect of the method of clearing the upper for treating the lower for treatment of chronic glomerulonephritis (CGN) complicated with tonsillitis and pharyngitis. Methods The changes of indices of 120 patients after treatment of herbal decoction were observed and analyzed. Results The Complete remission was 25 cases (20.8%), fundamental remission 42 cases (35%), improvement 38 cases (31.7%), and inefficacy 15 cases (12.5%). The total effective rate was 87.5%. The shorter the course of disease, the milder the pathological condition, the better the effect. The indices of qualitative examination of urinary protein and urinary RBC and WBC were significantly improved (P0.05). SCr and BUN of those patients with abnormal renal functions were all improved significantly after herbal treatment (P

OBJECTIVE:To establish a HPLC method for the determination of theophylline in pulsatile controlled-release pellets capsule of theophylline. METHODS: The determination was performed on a Diamonsil C18 column with the mobile phase consisted of methanol -water (35∶65) at a flow rate of 1.0 mL?min-1. The detection wavelength was set at 272 nm and the injection volume was 20 ?L. RESULTS: The linear range of theophylline was 5～40 ?g?mL -1 (r=0.999 9) with an average recovery rate of 100.55%. Both the intra-day RSD and the inter-day RSD were less than 1.2%. CONCLUSION: The method is accurate,reliable,simple and feasible,and it is applicable for the content determination of this preparation.

OBJECTIVE: To observe the clinical efficacy of nimodipine on cerebral infarction. METHODS: 120 patients with cerebral infarction were randomly selected into treatment group and control group(n=60). Both groups were given routine therapy, i.e. dehydration, neurotrophy, activating blood and dissolving stasis, lowering blood pressure, regulating blood lipid, lowering blood sugar and symptomatic treatment. Treatment group were additionally given oral dose of nimodipine 30 mg t.i.d. for 15 days. Clinical efficacies of 2 groups were observed and neurological impairment score before treatment and 15 days after treatment were evaluated. The change of hemorheology was detected by transcranial Doppler ultrasonography. RESULTS: The total effective rate of treatment group was 86.7%, which was significantly higher than that of control group(60.0%), there was significant difference between 2 groups(P