Objective:To introduce the use of fluoxetine in neurologic disease,especially in epilepsy.Method:We used fluoxetine as a supplement antiepileptic drug in 25 patients who couldnt be controlled yet by routine antiepileptics. A long term follow-up with these cases was carried out.Results:Most patients got better outcome.Conclusion:Fluoxetine would be an important supplement antiepileptic drug and have value for further clinical and experimental research.

Objective To establish HPLC method for fast, effective, simultaneous determination of Vancomycin and Norvancomycin in serum. Methods The separation was achieved on the Hypersil ODS2(250 mm ×4.6 mm,5μm)with methanol-acetonitrile-a mixture of potassium phosphate (9.5:2.5:88) as the mobile phase.The flow rate was 1.0 mL/min.The UV detecting wavelength was 280 nm.The temperature of the column was 35 ℃. Vancomycin and Norvancomycin were used as the internal standard for each other .Results Vancomycin and Norvancomycin blood concentration were in well linearity in the range of 1.25-100μg/mL,R2 =0.9996.The average method recovery rate of three concentrations were 96.88%,99.50%,105.01%respectively, the average method recovery rate of three concentrations were 94.40%,103.90,103.81%respectively, the intra-day and inter-day RSD were less than 5%.Conclusion The HPLC method is proved to be simple,sensitive, accurate and precise in which little blood sample is taken, thus it is suitable for the therapeutic drug monitoring(TDM) and pharmacokinetic study of Vancomycin and Norvancomycin.

To establish a method for the deter mination of dissolution of amiodarone hydrochloride tablets. Methods: Samples from five different factories were tested by the paddle meth od with the dissolving medium of 1% SLS. Results: The resu lts showed that there were very significant differences(P＜0.01) in dissolut ion parameters among the tested samples. Conclusion: It is necessary for testing the dissolution of the amiodarone product to control its quality and ensure clinical efficacy and safety.

Objective To investigate the marital status of male patients with breast cancer.Method Kansas marital satisfaction scale(KMS)was used in the investigation among 42 male patients with breast cancer.Results KMS score was (15.08±3.38),similar to the scores on normal model.More than 70% of them were satisfied with their spouses and marital relationship.Conclusions Male patients with breast cancer are satisfied with their marital status after surgery.Enhanced education on the disease knowledge to the patients and their families are important for their marital status.

With performance evaluation management as the stepping stone,the hospital joined the DRGs with the attending in-charge method by means of enforcing the attending in charge practice,DRGs knowledge training,and identifying problems with DRGs grouped data of the attending physician group, in an effort to explore new methods of medical quality control.Two years of practice provide tools of quality control to strengthen the hospital’s fine management.At the same time,it should also be noticed that DRGs merely diversify management means,as the content and form of which still need to constantly be improved in the course.

Objective To investigate the effects of iri doid on the spermatogenesis in mice of type-2 diabetes induced by nicotinamide and streptozotocin. Methods Fifty-six male C57BL mice were randomly divided into seven groups (n=8 for each group):control group, diabetic group, diabetic and glibenclamide (0.25 mg/kg) group, diabetic and iridoid (20 and 40 mg/kg) group, and iridoid (20 and 40 mg/kg) group. Diabetic model was induced by intraperitoneal injection of streptozotocin (65 mg/kg) and nicotinamide (120 mg/kg). Glibenclamide and iridoid were orally fed for 28 days. Twenty-four hours after the last treatment, blood samples, testis and the epididymis were collected for detecting blood hormones, testicular biopsy, and the evaluation of sperm parameters. Results The body weights of diabetic mice were recovered from the reduction after treatment with 40 mg/kg of iridoid and glibenclamide(P<0.05). The sperm count and viability were significantly decreased in diabetic group compared with those of control group (P<0.05). The sperm count was significantly increased in diabetic and glibenclamide group than that of diabetic group (P<0.05). The serum levels of lutein hormone (LH), follicle stimulating hormone (FSH) and testosterone (T) were significantly decreased in diabetic group compared with those of control group (P<0.05). The serum levels of LH, FSH and T were increased after treatment with 40 mg/kg of iridoid and glibenclamide (P<0.05). Conclusion These results suggest that iridoid can reduce the level of oxidative damage in
diabetic mice, and effectively improve the fertility of mice.

Xi Jinping,Secretary General has recently called on scientists mastering knowledge and proving in practice,to write papers in the motherland and in the realization of modernization.Up to the beginning of this century,ancestors and predecessors in Keshan disease prevention and control have made great achievements,including therapeutic methods,prevention of Keshan disease by supplementing selenium and compiling health standards,and these achievements have played an important technical supporting role in effectively controlling the epidemic of Keshan disease and guiding the practice of national wide control of Keshan disease.In the past 10 years,we have continued improving the technical norms to guide the work of national prevention and control of Keshan disease,and conducting translational research in response to the challenges of national Keshan disease prevention and control emerged in the new era.To realize the China dream of Keshan disease prevention and control,we must strive to achieve fully and sustainable elimination of Keshan disease,and to summarize the experiences in Keshan disease elimination for generalizing in other disease control and elimination,to continue to write the big article of the China dream of Keshan disease prevention and control.

Ubiquitin-specific proteases (USPs) regulate the deconjugation of ubiquitin tag from substrate proteins. Dysregulation of USPs has been linked to many diseases. Despite extensive efforts by academia and industry,high quality chemical molecules targeting the USPs family of ubiquitin enzymes are still scarce. In this commentary,I propose the criteria of a high quality chemical probe and the strategies to target USPs.

Objective To observe the clinical effect of Zhongfeng-Tengtong decoction combined with tramadol in the treatment of central post-stroke pain (CPSP). Methods A total of 121 patients with stroke were recruited in our hospital. The patients were randomly divided into 2 groups by the ramdom number table method, 61 patients in the treatment group and 60 in the control group. All patients received conventional treatment of stroke secondary prevention, and the control group received oral tramadol hydrochloride sustained release tablets, while the treatment group received Zhongfeng-Tengtong decoction based on the intervention of control group. Both groups were treated for 14 days. The outcomes included Numerical Rating Scale (NRS), Fugl-Meyer Assessment (FMA), Stroke Specific Quality of Life Scale (SS-QOL), and the changes of hemorheology indexes of the 2 groups before and after treatment. Results After treatment, the NRS scores of treatment group were significantly lower than the control group (2.5 ± 0.4 vs. 4.6 ± 0.7;t=20.303, P<0.01). and FMA and SS-QOL scores of treatment group were significantly higher than the control group (FMA 57.1 ± 18.1 vs. 44.2 ± 12.5;t=4.702, P<0.01), (SS-QOL 193.4 ± 25.6 vs. 165.4 ± 22.8;t=6.536, P<0.01). The whole blood viscosity, plasma viscosity, fibrinogen and thrombosis coefficient of treatment group were significantely lower than the control group (whole blood viscosity 4.22 ± 0.13 mPa?s vs. 4.56 ± 0.41 mPa?s, t=14.452, P<0.01;plasma viscosity 1.72 ± 0.31 mPa?s vs. 2.33 ± 0.23 mPa?s, t=14.258, P<0.01; fibrinogen 3.72 ± 0.49 g/L vs. 4.98 ± 0.41 g/L, t=17.783, P<0.01; thrombosis coefficient 0.76 ± 0.30 vs. 1.11 ± 0.20, t=8.178, P<0.01). Conclusions Zhongfeng-Tengtong decoction combined with tramadol could alleviate the pain symptom in patients with CPSP, improve the movement function of limbs and improve the quality of life.

To observe the therapeutic effect of acupuncture plus TDP irradiation on proliferative arthritis, 304 patients with proliferative arthritis were randomly divided into treatment group and control group, and treated by acupuncture plus TDP irradiation and routine Tuina respectively. The clinical cure rate and total effective rate in the treatment group were both superior to those in the control group. This therapy has good therapeutic effect and short course of treatment in treating proliferative arthritis, and is one of better therapeutic means in clinic presently.